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Citations for Thermo Fisher :
5201 - 5250 of 10000+ citations for Monkey Caveolin 1 CAV1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Supernatants were collected and measured for protein concentration using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2024Quote: Protein concentration in the samples was determined with a BCA assay (Pierce BCA Protein Assay Kit; Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... 6000 rpm 4°C and protein concentration was estimated by BCA protein assay kits (Thermo-Fisher Scientific, Cat #23225).
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bioRxiv - Microbiology 2024Quote: ... The concentrations of the protein extracts were determined using a BCA assay (Pierce BCA Protein Assay, Thermo Fisher Scientific) and a verification of the protein extracts was carried out on a 12.5% acrylamide SDS-PAGE gel stained with Coomassie brilliant blue R-250 ...
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bioRxiv - Cancer Biology 2024Quote: ... The total protein concentration of each cell lysate was determined with a BCA protein assay kit (Thermo Fisher, # 23225). For each sample ...
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bioRxiv - Microbiology 2024Quote: ... The concentration of the protein was estimated using the BCA kit (Pierce BCA Protein Assay Kit - Thermo Fisher Scientific) according to the supplier’s recommendations as well as by comparing the protein on a 12.5% acrylamide Coomassie-stained SDS-PAGE gel with BSA standards ...
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bioRxiv - Synthetic Biology 2024Quote: ... Total protein content was quantified using PierceTM BCA protein assay kit as per the supplier’s protocol (Thermo Scientific, USA). For albumin ...
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bioRxiv - Molecular Biology 2024Quote: ... 15 µl (for ChIP) or 100 μl (for ChIP-seq) Dynabeads Protein A and Protein G (Thermo Fisher Scientific) were pre-incubated overnight with rotation in the presence of 5 mg/ml BSA and 3 µg (for ChIP ...
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bioRxiv - Bioengineering 2024Quote: Protein samples were diluted in PBS to 5 μM and mixed with 5000× SYPRO Orange Protein Gel Stain (Invitrogen) to final 1.25x concentration ...
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bioRxiv - Molecular Biology 2024Quote: ... and the resulting supernatant was assayed for protein concentration with a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) and then subjected to SDS-polyacrylamide gel electrophoresis on a 10% acrylamide/4% bisacrylamide gel ...
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bioRxiv - Cell Biology 2024Quote: ... Peak measurements were normalized to protein content of duplicate samples as determined by BCA protein assay (Thermo Fisher Scientific). All experiments were performed independently at least three times and a representative experiment is shown.
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bioRxiv - Neuroscience 2024Quote: ... Supernatants were collected and the total amount of proteins was calculated by the BCA Protein Assay kit (ThermoFisher Scientific) using a BSA standard curve ...
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bioRxiv - Neuroscience 2024Quote: ... the samples underwent normalization based on protein content using the BCA protein assay kit (#23225, Thermo Scientific Pierce, US) per the manufacturer’s instructions.
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bioRxiv - Biochemistry 2024Quote: ... and total protein concentrations of cleared cell lysates were measured with the PierceTM BCA Protein Assay Kit (ThermoFisher Scientific) using BSA as a standard ...
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bioRxiv - Biochemistry 2024Quote: Protein in mouse and human wound fluid samples were quantified with the BCA Protein Assay kit (Thermo Fisher Pierce) using the 96-well plate protocol recommended by the manufacturer ...
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bioRxiv - Neuroscience 2024Quote: ... Total protein levels in all lysates were quantified using PierceTM BCA Protein Assay Reagents (Thermo Fisher: #23222 and #23224). After the incubation step for the poly-GP immunoassay ...
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bioRxiv - Microbiology 2024Quote: ... The protein concentration in each sample was determined using the BCA protein assay kit (Thermo Fisher Scientific, Waltham, USA) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... and total protein concentrations were quantified using a Pierce™ BCA Protein Assay Kit (ThermoFisher Scientific, Waltham, MA, USA). Samples for gel electrophoresis were prepared by boiling in loading buffer for 10 min ...
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bioRxiv - Systems Biology 2024Quote: ... The protein concentrations in both supernatants (SOL and SDS) were determined with Pierce BCA Protein Assay Kit (Thermo Scientific).
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bioRxiv - Molecular Biology 2024Quote: ... Lysate was clarified by centrifugation and total protein concentration was measured with the BCA Protein Assay (Thermo Fisher Scientific). Samples were electrophoresed and transferred to a 0.45 μm nitrocellulose membrane (Bio-Rad) ...
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bioRxiv - Cell Biology 2024Quote: ... Equal amounts of protein were supplemented with 150mM NaCl and incubated with Protein A/G Magnetic Beads (ThermoFisher #88802) preloaded either or not with Golgin-97 antibody ...
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bioRxiv - Molecular Biology 2024Quote: ... Lysate was clarified by centrifugation and total protein concentration was measured with the BCA Protein Assay (Thermo Fisher Scientific). Samples were electrophoresed and transferred to a 0.45-μm nitrocellulose membrane (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: ... and TAP-tagged proteins (TAP-Csm4, TAP-Mps2, and TAP-Mps3) were detected by an anti-TAP rabbit antibody (1:10,000, Thermo Fisher Scientific, cat#CAB1001). The level of Pgk1 was detected by a Pgk1 antibody (1:10,000 ...
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bioRxiv - Molecular Biology 2021Quote: ... The diluted protein was coated in an increasing gradient (25-200 ng well-1) on a 96-microtiter ELISA plate (Nunc, Thermo Fisher Scientific) overnight at 4°C ...
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bioRxiv - Immunology 2021Quote: ... then permeabilized and stained with antibodies for intracellular protein for 1 h using the Foxp3 Staining Buffer Set (Thermo Fisher Scientific, Waltham, MA). Data were collected in a FACSCalibur flow cytometer (BD Biosciences ...
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bioRxiv - Systems Biology 2020Quote: Peptides from brain digests used for the first 3 batches of the untargeted UPenn cohort analysis (equal to 1 µg protein digestion) were used for targeted analysis on an Orbitrap Lumos™ Tribrid™ Mass Spectrometer (ThermoFisher Scientific) fitted with a Nanospray Flex ion source and coupled to a NanoAcuity liquid chromatography system (Waters) ...
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bioRxiv - Cell Biology 2019Quote: ... Following washes by 0.1% Tween-20 in TBS the detection of the target proteins was carried out using Super Signal West Femto (Thermo Fisher Scientifics, 34095) and ChemiDoc imaging system (BioRad).
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bioRxiv - Cell Biology 2019Quote: ... Following washes by 0.1% Tween-20-1X TBS the detection of the target proteins was carried out using Super Signal West Femto (Thermo Fisher Scientifics, 34095) and ChemiDoc imaging system (BioRad).
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bioRxiv - Bioengineering 2020Quote: ... the gel was washed three times for 10 min in water followed by a 1 h staining period with PageBlue® Protein Staining Solution (Thermo Fisher Scientific). For MS analysis gel bands corresponding to high molecular weight range proteins were excised ...
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bioRxiv - Biophysics 2022Quote: ... The 7 mm wells so created were coated with a 0.1 mg.mL−1 solution of GIBCOTM Fibronectin Bovine Protein (Thermo Fisher Scientific, Boston, MA, USA) in PBS (pH 7.4 ...
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bioRxiv - Immunology 2022Quote: ... These cells were cocultured with A549-ACE2-A2402 cells expressing each spike protein an E:T ratio of 2:1 and incubated with RPMI 1640 medium (Thermo Fisher Scientific, Cat# 11875101) containing 10% FBS at 37°C for 6 h ...
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bioRxiv - Genomics 2021Quote: ... Membranes with liver and muscle mitochondrial protein samples were incubated overnight with COX4 antibody (1:1.000 COX4 Polyclonal Antibody, cat no. PA5-17511, Thermo Fisher Scientific, Rockford, USA) and loading control protein ATP5A antibody (1:1.000 anti-ATP5A antibody ...
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bioRxiv - Cell Biology 2020Quote: ... Following washes by 0.1% Tween-20-1X TBS the detection of the target proteins was carried out using Super Signal West Femto (Thermo Fisher Scientifics, 34095) and ChemiDoc imaging system (BioRad).
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bioRxiv - Neuroscience 2020Quote: Nuclear fractionation from hippocampus of 30-week old wild-type and R6/1 mice was performed using a Subcellular Protein Fractionation Kit for Tissues (Thermo Fisher Scientific #87790) following manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Protein was isolated from tissues by sonication (40% amp) in lysis buffer (100:1 T-PER Tissue Protein Extraction Reagent and Halt Protease and Phosphatase Inhibitor Cocktail; Thermo Scientific, Waltham, MA). Homogenized samples were then incubated for 30 min at 4°C ...
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bioRxiv - Neuroscience 2020Quote: Brain cortex samples were lysed in 1 mL lysis buffer (20 mM HEPES pH 7.9, 1% SDS, and protease inhibitor) with protein concentrations determined by BCA assay (Thermo Fisher Scientific, Cat # 23225). Reduction and alkylation were performed as previously described50 ...
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bioRxiv - Cell Biology 2022Quote: ... Breg adenovirus-transduced brown adipocytes were cultured in serum-free medium with 1× protein transport inhibitor cocktail (10.6 µM Brefeldin A and 2 µM Monensin) (Fisher scientific, #50-930-9), 10 µM oligomycin ...
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bioRxiv - Genetics 2024Quote: ... 1:10 dilution of lysates in LP2 buffer were used to quantify protein content using Pierce BCA Assay Kit (Thermo Fisher Scientific #23227). The resulting 2′3′-cGAMP levels were normalized to protein content in each sample.
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bioRxiv - Cell Biology 2023Quote: ... Click-iT reaction buffer additive 1 and Click-iT reaction buffer additive 2 from the Click-iT™ Protein Reaction Buffer Kit (Thermo Fisher Scientific) were added to the sample according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The membranes were probed with an anti-SARS-CoV-2 spike protein S1/S2 polyclonal antibody (1:1,000, Thermo Fisher Scientific PA5-112048), and an anti-p24 monoclonal antibody (1:1,000) ...
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bioRxiv - Molecular Biology 2023Quote: Total protein lysates were immunoblotted onto nitrocellulose membranes and incubated with the following primary antibodies: anti-ELP3 (ThermoFisher, Cat.702669, 1:200 dilution), anti-eIF2α (Cell Signalling Cat.9722 ...
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bioRxiv - Genetics 2023Quote: ... One-cell stage embryos were injected with ∼1 nl solution containing 100 pg of gRNA and 200 pg of GeneArt Platinum Cas9 protein (Thermo Fisher Scientific, USA). Injected embryos were raised into adults ...
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bioRxiv - Neuroscience 2023Quote: ... Brain extracts (6μg of total protein from the PBS-soluble fraction) were then incubated with Lipofectamine 2000 (Invitrogen, final concentration 1% vol/vol) in opti-MEM for 10 min at room temperature before being added to the cells ...
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bioRxiv - Biophysics 2024Quote: ... protein fractions were mixed with SDS-free loading buffer in a 3:1 ratio and loaded on a Novex™ 4–20% Tris-Glycine Gels using NativeMark™ Unstained Protein Standards (Invitrogen™) (range from 20 to 1236 kDa ...
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bioRxiv - Plant Biology 2020Quote: ... The protein content of the supernatant was quantified using Ready-to-use Coomassie Blue G-250 Protein Assay Reagent (ThermoFisher) and Albumin Standard 23209 (ThermoFisher).
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bioRxiv - Plant Biology 2019Quote: ... GTPase activity of native and mutant Gα proteins were detected with or without an RGS protein using the ENZchek phosphate assay kit (Invitrogen) as previously reported (Roy Choudhury et al. ...
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bioRxiv - Cancer Biology 2021Quote: The cell membrane protein samples were collected using Mem-PER eukaryotic membrane protein extraction reagent kit (Thermo Scientific, 89826, MA) according to the protocol instruction ...
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bioRxiv - Biophysics 2021Quote: ... The crude lysates were cleared by centrifugation at 13,000 rpm for 15 min and protein content was analyzed by Pierce BCA protein assay (Thermo Scientific).
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bioRxiv - Cancer Biology 2021Quote: ... After clearing by centrifugation at 13,300□rpm for 10□min at 4°C the protein concentration was determined using Coomassie Plus Protein Assay (ThermoFisher Scientific). Protein concentrations were adjusted to a final concentration of 2mg/ml followed by denaturation upon addition of 4X sample buffer containing 10% Beta-mercaptoethanol and heating to 95oC for 5 minutes ...
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bioRxiv - Cancer Biology 2021Quote: Subcellular protein fractionation was performed either by using Subcellular Protein Fractionation Kit for Cultured Cells (Thermo Scientific; Cat. no. 78840) according to the manufacturer protocol or following the protocol below.