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Citations for Thermo Fisher :
5201 - 5250 of 10000+ citations for Glutathione Fluorescent Detection Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... proteins were revealed by detection of HRP with the SuperSignal West Pico PLUS chemiluminescent substrate (ThermoFisher). The images were detected by the Syngene PXi image analysis system and analyzed using ImageJ software (NIH).
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bioRxiv - Cell Biology 2023Quote: ... and 10 ng of reverse-transcribed cDNA in the PRISM 7500 Sequence Detection System (Applied Biosystems). The thermal-cycler protocol was ...
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bioRxiv - Developmental Biology 2023Quote: ... Secondary detection was performed with antibodies conjugated to Alexa Fluor488 (Invitrogen A-11039 or Abcam ab150129), Alexa Fluor594 (Jackson ImmunoResearch 711-585-152) ...
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bioRxiv - Cancer Biology 2023Quote: ... and real-time detection and quantification of cDNAs was performed on the Viia7 Cycler (Applied Biosystems) with 40 cycles of amplification ...
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bioRxiv - Microbiology 2023Quote: ... with slit width of 10 e-V and Falcon 4 direct detection camera (Thermo Fisher Scientific) at ×165,000 magnification ...
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bioRxiv - Genetics 2024Quote: ... Genotype data were automatically acquired and analyzed using sequence detection software (SDS v1.3.1; Applied Biosystems, Inc.).
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bioRxiv - Microbiology 2024Quote: ... MS detection was conducted using a Q-Exactive Orbitrap mass spectrometer (Thermo Fisher Scientific, Waltham, MA) equipped with a Nano Electrospray Ionization probe ...
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bioRxiv - Bioengineering 2024Quote: ... DNA samples designated for mycoplasma detection were diluted with nuclease free water (Gibco; cat# 10977-015) to obtain 15ng DNA per reaction well ...
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bioRxiv - Cell Biology 2024Quote: ... Detection was done using HRP-conjugated secondary antibodies and SuperSignal West Pico Chemiluminescent substrate (Thermo Fisher), or DyLight conjugated antibodies (ThermoFisher ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was then diluted 1:5 in 1x Assay buffer and processed according to the kit instructions (DNA Damage Competitive ELISA Kit, Invitrogen). The initial DNA concentration used in the assay was optimized to ensure the resulting values fall within the optimum range of kit detection limits (between Std3 and Std6) ...
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bioRxiv - Microbiology 2022Quote: IL-8 and IL-1β cytokines were quantified by ELISA using the Rabbit IL-1 beta ELISA Kit and Rabbit IL-8 ELISA Kit (Invitrogen). Aliquots of 2 mL of lung homogenate shred in saline solution were centrifuged during 30 min at 10 000 rpm and 4°C ...
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bioRxiv - Molecular Biology 2023Quote: The TaqMan assays were performed using a one-step TaqMan probe kit (TaqManTM RNA-to CTTM 1-Step Kit, Invitrogen). As the TaqMan probe we used 12➔7 Probe FAM–ACCATCAGCCCCCTTTTTATTTCCT-MGBNFQ ...
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bioRxiv - Cell Biology 2020Quote: Calibration Optical Transfer Functions (OTFs) were created by imaging single isolated 175 nm fluorescent beads (Thermo Fisher PS-Speck), with no other bead within 30 µm ...
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bioRxiv - Cell Biology 2020Quote: ... the Fibrinogen anchor in the above protocol was replaced by the protein of interest at a concentration of 50 μg/ml in 0.1 M sodium bicarbonate pH 8.3 (except for fluorescent EGF, where biotinylated EGF complexed to streptavidin-Alexa555 (Invitrogen, E35350) was pattered at 1 μg/ml in 0.1 M sodium bicarbonate ...
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bioRxiv - Developmental Biology 2021Quote: ... The ORF of red fluorescent protein gene (RFP)-fused Antp was inserted into a pIZ/V5-His vector (Invitrogen) driven by the OpIE2 promoter ...
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bioRxiv - Neuroscience 2022Quote: ... Fluorescent indicators (fluo-4 AM, fura-2 AM, fura-FF AM) were purchased from Molecular Probes (Thermo Fisher Scientific). NMDA was from Merck Millipore ...
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bioRxiv - Neuroscience 2021Quote: ... Viral infusion into the OFC or DLS was visualized using an EVOS FL tabletop fluorescent microscope (ThermoFisher; Waltham, MA).
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bioRxiv - Molecular Biology 2021Quote: ... cells were infected with baculovirus expressing red fluorescent protein targeted to mitochondria (CellLight Mitochondria-RFP, BacMam 2.0, Life Technologies). To visualize the nucleus ...
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bioRxiv - Biophysics 2019Quote: ... the neurons were incubated with anti-mouse IgG conjugated to fluorescent Alexa Fluor 568 (A-11004, Thermo Fisher Scientific) or Alexa Fluor 488 (A-11001 ...
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bioRxiv - Cell Biology 2019Quote: ... For the injection at high concentration (to have aggregates formation) of latex fluorescent beads (0.1 µm; Life Technologies, F8803), the beads were rinsed several times in nuclease-free water before use to remove traces of sodium azide and diluted 10 times before injection.
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bioRxiv - Biophysics 2019Quote: ... Splitter calibration was implemented by using a field of dense 200 nm fluorescent beads (ThermoFisher, dark red FluoSpheres F8807) scanning across the entire region of interest ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then washed three times in PBS before imaging with fluorescent microscope (EVOS FL, Life Technologies, Carlsbad, USA).
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bioRxiv - Bioengineering 2021Quote: ... The latter was obtained by scanning the line illumination over stationary 100 nm fluorescent beads (Thermo Fisher Scientific, F8803) sparsely deposited on a coverslip ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were loaded with culture medium containing 3 μM of the fluorescent calcium indicator Fluo-4/AM (Life Technologies) and Pluronic F-127 (0.2% w/v ...
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bioRxiv - Cell Biology 2020Quote: ... Glucose uptake assay was measured as previously described using the fluorescent D-glucose analog 2NBDG (200 μM; Molecular Probes)35 ...
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bioRxiv - Biophysics 2021Quote: ... 2 μl (0.4%) Carboxylate-modified fluorescent beads (FluoSpheres™ Carboxylate-Modified, 0.2 μm, 580/605; F8810, Thermo Fisher Scientific), 2.5 μl Ammonium Persulfate (0.05% ...
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bioRxiv - Biochemistry 2020Quote: In vivo Δψm was measured using the cell-permeant red-fluorescent dye TMRE (tetramethylrhodamine ethyl ester, Thermo Fisher Scientific). For each time point ...
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bioRxiv - Bioengineering 2021Quote: ... liposomes and EVs were labeled with fluorescent DiR (Invitrogen™ Molecular Probes™, Thermo Fisher Scientific, Waltham, MA, USA). To label PMSC-EVs ...
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bioRxiv - Biophysics 2021Quote: Endogenous reactive oxygen species (ROS) were quantified with the fluorescent stain CellROX Orange (Thermofisher, excitation/emission 545/565 nm) to determine impact of nutrient limitation on ROS production ...
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bioRxiv - Biophysics 2021Quote: ... as specified by the commercial protocol) and a fluorescent tag (Alexa Fluor® succinimidyl esters, Invitrogen™, Molecular Probes® ...
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bioRxiv - Cancer Biology 2020Quote: ... washed and stained with the vital cell tracker red fluorescent CM-Dil (4 ng/ul final concentration, Invitrogen, #C7001), following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... 2011) was applied using 15 nM control siRNA (BLOCK-iT Alexa Fluor red or green fluorescent oligo, Invitrogen, USA) and 30 nM Dnmt1 siRNA ...
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bioRxiv - Immunology 2021Quote: ... Biotinylated recombinant HAΔTM H1N1 A/Michigan/45/2015 and H3ΔTM H3N2 A/Hong Kong/4801/2014 were coupled to red fluorescent NeutrAvidin microspheres (ThermoFisher), incubated with sera and added to single wells as described above ...
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bioRxiv - Systems Biology 2021Quote: Yeast strains were stained with the red fluorescent dye FM4-64 (excitation/emission, 515/640 nm) (Thermo Fisher Scientific). Exponentially growing cells were incubated at an OD of 0.5-1 in YPD with 2 µM FM4-64 in the dark for 30 minutes at 30°C ...
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bioRxiv - Microbiology 2020Quote: ... EVs were resuspended in D-MEM (0 % FCS) + fluorescent Bodipy Phosphocholine analogue at 2 μg/mL (D3793, Thermofisher Scientific) and incubated overnight at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... The fluorescent labeling of PlyCBWT and PlyCBR66E was performed using the manufacturer’s recommended guidelines (Thermo Fisher, cat. No. A20174). Analytical gel filtration was used to determine the multimeric nature of PlyCB and PlyCBR66E ...
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bioRxiv - Microbiology 2021Quote: ... for 12 hours was measured using a fluorescent membrane potential sensitive dye called bis-(1,3-dibutyl barbituric acid)-trimethylene oxonol (Invitrogen). Briefly ...
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bioRxiv - Physiology 2021Quote: ... diluted in 98μl 0.9% NaCl and stained with 0.25μl SYTO-16 Green Fluorescent Nucleic Acid Stain (ThermoFisher Scientific, catalog # S7578) within 4 hours of collection ...
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bioRxiv - Microbiology 2021Quote: ... images were corrected for chromatic aberration as previously described57 using a sample of 0.1 µm TetraSpeck multi-colour fluorescent beads (ThermoFisher) adhered to glass as a reference sample ...
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Mitochondrial and metabolic remodeling in human skin fibroblasts in response to glucose availabilitybioRxiv - Cell Biology 2021Quote: General intracellular oxidative stress was determined by following the oxidation of the CM-H2DCFDA fluorescent dye (Molecular Probes, Invitrogen). NHDF cells grown in HGm ...
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Mitochondrial and metabolic remodeling in human skin fibroblasts in response to glucose availabilitybioRxiv - Cell Biology 2021Quote: General intracellular oxidative stress was determined by following the oxidation of the CM-H2DCFDA fluorescent dye (Molecular Probes, Invitrogen). NHDF cells grown in HGm ...
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bioRxiv - Biophysics 2020Quote: ... Gels that were indicated for traction force microscopy were supplemented with additional 0.2 μm yellow-green fluorescent (505/515) microspheres (~1.5 × 1010/ml final concentration, Invitrogen, F8811), which were sonicated for 3 min prior to use ...
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bioRxiv - Biophysics 2020Quote: ... 0.5 kPa and 22 kPa acrylamide prepolymer solutions were prepared and 0.1 μm fluorescent (505/515) microspheres (~1.2 × 1011/ml final concentration, Invitrogen, F8803) were added to the 22 kPa solution ...
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bioRxiv - Biophysics 2020Quote: A1-LCD was fluorescently labeled on the N-terminus using Alexa Fluor® 488 NHS ester fluorescent dye (ThermoFisher) to label the primary amines of proteins ...
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bioRxiv - Biophysics 2020Quote: ... The grids were subsequently coated with 100 nm and 500 nm fluorescent microspheres (TetraSpeck Microspheres, Invitrogen T7279, T7281, USA) at a 500x or 1000x dilution for 5 min ...
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bioRxiv - Biochemistry 2022Quote: ... Cells remained in the dark with all fluorescent indicators followed by trypsinization and one wash with 1X PBS (Gibco). The indicators employed and fluorescence excitation/emission wavelengths were as follow ...
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Dissociation of β2m from MHC Class I Triggers Formation of Noncovalent, Transient Heavy Chain DimersbioRxiv - Cell Biology 2022Quote: ... After aligning ATTO 643 and ATTO Rho11 channels with sub-pixel precision through spatial transformation based on a calibration measurement with multicolor fluorescent beads (TetraSpeck microspheres 0.1 μm, Invitrogen), individual molecules detected in both spectral channels of the same frame within a distance threshold of 100 nm were considered to be co-localized ...
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bioRxiv - Immunology 2022Quote: ... and soluble anti-CD28 (1µg/ml) for the indicated time before harvesting and staining with near-IR fluorescent reactive dye (Invitrogen).
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bioRxiv - Systems Biology 2022Quote: ... and histone 2B-green fluorescent protein (H2B-GFP) were cultured with Dulbecco’s Modified Eagle Medium – High Glucose (DMEM; Gibco) supplemented with 10% fetal bovine serum (Omega Scientific) ...
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bioRxiv - Biophysics 2022Quote: ... Samples were incubated for 1h with a filtered and sonicated solution of red fluorescent carboxylate-modified beads (FluoSpheres, Invitrogen) of 100nm (220nm filter ...