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Citations for Thermo Fisher :
5151 - 5200 of 9391 citations for Mumps Virus Nucleoprotein Strain L Zagreb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... and DMEM/F12 (1:1) 1x (+) L-Glutamine (11320-033, Gibco) was added ...
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bioRxiv - Biochemistry 2024Quote: ... or supplemented with only 3 mM L-glutamine (Gibco, 25030-024) was added to the cells ...
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bioRxiv - Cell Biology 2023Quote: Cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM 1X, containing pyruvate and 4.5 g/L D-glucose, L-glutamine from Gibco, Life Technologies Billings, MT, USA, cat. no. 41966-029) supplemented with 10% heat-inactivated foetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2022Quote: ... secondary antibodies Alexa Fluor® 488 Donkey Anti-Mouse IgG (H+L) and/or Alexa Fluor® 647 Donkey Anti- Mouse IgG (H+L) (Life Technologies A21202 and A31571, respectively) were added diluted in PFS (1:1,000 ...
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bioRxiv - Microbiology 2020Quote: ... Pyrene-labeled virus was produced in BHK-21 cells cultured beforehand in the presence of 15 μg/ml 1-pyrenehexadecanoic acid (Thermo Fisher Scientific, Waltham, MA, USA). The supernatant was harvested at 48 hpi ...
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bioRxiv - Microbiology 2022Quote: ... plasmid encoding myc- and His-tagged full-length EBOV GP (Zaire ebolavirus isolate Ebola virus/H.sapiens-wt/SLE/2014/Makona-G3838) using Lipofectamine™ 3000 (Thermo Fisher Scientific Cat. Nr. L3000) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... Infiltrated leaves were collected 5 days after infiltration and homogenised with virus inoculation buffer (10 mM monopotassium phosphate containing 1% Celite (Thermo Fisher Scientific, 68855-54-9)) ...
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bioRxiv - Plant Biology 2024Quote: Relative virus accumulation was measured on five biological replicates using AgPath-IDTM One-step RT-PCR Kit for TaqMan chemistry (Applied Biosystems by Thermo Fisher Scientific). The third upper systemically infected leaf from each source plant was collected at 21 dpi ...
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bioRxiv - Neuroscience 2024Quote: ... A total of 50 µg of proviral plasmid was transfected into 107 HEK293T/17 cells for virus production using Lipofectamine 2000 (Thermo Fisher Scientific, Cat# 11668-027). The next day ...
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bioRxiv - Microbiology 2020Quote: ... burgdorferi strains grown under mammalian-like conditions were used for reverse transcription into cDNA using random primers (Thermo Fisher Scientific, Waltham, MA) and SuperScript III (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... burgdorferi strains grown under mammalian-like conditions were used for reverse transcription into cDNA using random primers (Thermo Fisher Scientific, Waltham, MA) and SuperScript III (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... non-exposed and Dongola strain mosquitoes were used to synthesize cDNA using the High-Capacity cDNA Reverse Transcription kit (Applied Biosystems, USA) with oligo-dT20 (New England Biolabs ...
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bioRxiv - Developmental Biology 2019Quote: ... Total RNA was extracted from pooled embryos from heterozygote intercrosses or AB strain WT (n=20-30) using Trizol (Life Technologies, 15596). cDNA was prepared with SuperScript III/IV Reverse Transcription Kit (Life Technologies ...
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bioRxiv - Microbiology 2019Quote: ... 5 μg of total RNA (harvested at late-exponential phase, biological triplicates) from each strain was reverse transcribed with Superscript IV reverse transcriptase (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... N-terminally acetylated and phosphorylated α-Syn strains were cleared from endotoxins using one run of Detoxi-Gel Endotoxin Removing Gel (Thermo Scientific) or until endotoxins were below detection level ...
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bioRxiv - Microbiology 2021Quote: ... The mutations for Alpha and Beta (Fig. 4A) were added in silico to the codon-optimized Wuhan strain and ordered as synthetic genes (GeneArt, Thermo Fisher Scientific) and cloned into the same backbone (Planas et al. ...
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bioRxiv - Microbiology 2020Quote: ... sequences of the tprL and tp0574 genes were amplified from Nichols DNA using primers conserved across all strains and cloned into a pCRII-TOPO vector (Thermo Fisher Scientific). For tprL ...
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bioRxiv - Microbiology 2021Quote: OmRV-fragment1/pMK and OmRV-fragment2/pMA plasmids designed on the basis of the full genome sequence of the OmRV AK4 strain (GenBank accession number: AB555544) were ordered from GeneArt Custom Gene Synthesis Service (Thermo Fisher Scientific). OmRV-fragment1/pMK (kanamycin resistant) ...
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bioRxiv - Microbiology 2022Quote: ... The strain was streaked on Luria-Bertani plates and cultured in non-cation adjusted Mueller-Hinton Broth (MHB, BD DifcoTM, Thermo Fisher Scientific), with aeration at 37°C and 220 rpm shaking.
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bioRxiv - Microbiology 2022Quote: ... The mutations for Alpha and Delta were added in silico to the codon-optimized Wuhan strain and ordered as synthetic genes (GeneArt, Thermo Fisher Scientific) and cloned into the same backbone ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant bacmid DNA that carried the NA ectodomain from the strain of interest was generated using the Bac-to-Bac system (Thermo Fisher Scientific). Baculovirus was generated by transfecting purified bacmid DNA into adherent Sf9 cells using Cellfectin reagent (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... Cells were transfected with 2 μg of phCMV3 plasmids encoding the C-terminal HA-tagged NA from the strain of interest using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... of the SARS coronavirus strain Tor2 (NC_004718.3) was ordered as a synthetic DNA string fragment (GeneArt synthesis; Thermo Fisher Scientific, Darmstadt, Germany) and cloned into the pEXPR103 expression vector (IBA Lifesciences ...
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bioRxiv - Microbiology 2019Quote: ... trophozoites were the starting strains used in these studies.10 These and all the newly transfected strains were grown in TYI-S-33 medium supplemented with 10% bovine serum and 0.05 mg/mL bovine bile without (wild type WBC6) or with (transfected strains) antibiotic selection pressure of 32 µg/mL puromycin dihydrochloride (Gibco, Dublin, Ireland).10 ...
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bioRxiv - Immunology 2022Quote: ... aegypti (Liverpool strain) female mosquitoes were intrathoracically injected with 69 nl of LPS (1 mg/ml) [Sigma Aldrich] or dPBS (Thermo Fisher Scientific) using a Nanoject III injector (Drummond Scientific Company ...
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bioRxiv - Immunology 2022Quote: ... fumigatus strains Af293 and CEA10 were grown at 37℃ for 5 days on Sabouraud’s Dextrose Agar (SBD; Difco, Fisher Scientific, Rockford, IL) or for 3 days on glucose minimal media slants (GMM) ...
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bioRxiv - Microbiology 2022Quote: ... The viral genome between 27,895-29,534 nucleotides based on the SARS-CoV-2 WA-1 strain was RT-PCR amplified using Super Script II Reverse transcriptase (Thermo Fisher Scientific) and Expanded High Fidelity PCR system (Sigma Aldrich) ...
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bioRxiv - Synthetic Biology 2020Quote: ... Amplified constructs were then transformed into the reconstructed EC strain and plated on Yeast Extract Peptone Dextrose (YPD) with Geneticin (200 μg / mL; Gibco™ 10131035). Independent colonies were screened via PCR using primers that annealed 200 bp outside of the PCR-generated homology region ...
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bioRxiv - Microbiology 2020Quote: ... FA at 250 nL min-1 over 260 min (samples from single strain cultures) or 140 min (SCX fractionated samples from co cultures) and analysed on a Q-Exactive instrument (Thermo Fisher Scientific) run in a data-dependent manner using a “Top 10” method ...
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bioRxiv - Synthetic Biology 2021Quote: ... the library and query strains were arrayed in 384 colony format on YES agar containing 250 µg/ml G418 (Geneticin; Life Technologies, 10131027) or 100 µg/ml ClonNat (Nourseothricin ...
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bioRxiv - Immunology 2020Quote: SARS-CoV-2 strain NMC-nCoV02 (reference, Cell host & Microbe) was propagated in Vero cells in Dulbecco’s Modified Eagle Medium (DMEM; Gibco, Grand Island, NY) supplemented with 1% penicillin/streptomycin (GIBCO ...
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bioRxiv - Microbiology 2019Quote: ... and SLKP cells (a pool of long-term infected single cell clones derived from SLK cells infected with the BCBL1 KSHV strain) [4] were grown in DMEM High Glucose (Gibco, Darmstadt, Germany) supplemented with 10% FCS ...
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bioRxiv - Immunology 2021Quote: ... Spike mutations were added in silico into the Wuhan codon optimized reference strain and were ordered as synthetic genes (GeneArt - Thermo Fisher Scientific) and transferred into the phCMV backbone ...
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bioRxiv - Immunology 2020Quote: Wild-type Phl p 6 and its mutants were expressed from pET17b constructs in Escherichia coli strain BL21 Star (DE3; Invitrogen/Thermo Fisher Scientific), as described in the supplement.
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bioRxiv - Systems Biology 2021Quote: ... the DNA of the Rps22B-GFP and Rps9A-GFP strains from the library was extracted using Yeast DNA Extraction Kit (Thermo Scientific, 78870). PCR Amplification of the GFP label with 40 base pairs flanking sequences was done using the corresponding F2 and R1 pair of primers from Huh et al (https://yeastgfp.yeastgenome.org/yeastGFPOligoSequence.txt ...
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bioRxiv - Microbiology 2020Quote: ... and from original strains (C2865, C2853, C2854, C2855) were digested with 1 Unit of S1 enzyme (1 min at 37°C) (Thermo Fisher Scientific) for plasmid linearization ...
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Guidelines for accurate genotyping of SARS-CoV-2 using amplicon-based sequencing of clinical samplesbioRxiv - Genomics 2020Quote: ... The number of viral genome copies for the strain specified in the experiment was spiked into 50 ng of Universal Human Reference RNA (ThermoFisher Scientific #QS0639) to the final volume of 11 μl ...
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bioRxiv - Biochemistry 2022Quote: ... The raw data was searched against the Mycolicibacterium smegmatis (strain ATCC 700084 / MC2 155) UniProt Reference Proteome (Proteome ID UP000000757) using Sequest HT (University of Washington and Thermo Fisher Scientific). Iodoacetamide-mediated cysteine carbamidomethylation was set as a static modification ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were strained through a 35 µm strainer and incubated at 37°C for 30 minutes with 0.5 µL Vybrant™ Dyecycle™ Ruby strain (Invitrogen, V10309) per mL of FANS buffer.
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bioRxiv - Molecular Biology 2024Quote: Wild type genomic DNA of Mycobacterium tuberculosis strain H37Ra was obtained from ATCC (Cat#25177DQ) and quantified by Nanodrop spectrophotometer (Thermo Fisher Scientific). DNA fragments with wild type or mutation MTB DNA sequences were synthesized and cloned into pUC19 plasmid by GenScript (Figure 2) ...
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bioRxiv - Genomics 2024Quote: ... The resulting 6x-MBP-hBPTF (701-1073) fusion protein was expressed in the Escherichia coli host strain BL21(DE3) (Thermo Fisher Scientific), and the soluble protein was purified on a HisTrap FF column (Cytiva) ...
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bioRxiv - Microbiology 2023Quote: ... with the resulting plasmid confirmed as correct by Sanger sequencing (Genewiz). The Escherichia coli (E. coli) DH5-α strain (Thermo Fisher Scientific) was used for plasmid amplification ...
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bioRxiv - Molecular Biology 2023Quote: ... coli EPI300 strains were cultured at 37 °C and at 250 rpm in the Luria broth medium (Fisher Scientific, Pittsburgh, PA, USA).
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bioRxiv - Cell Biology 2023Quote: ... Procyclic cells (PCF) derived from Trypanosoma brucei subspecies brucei strain Lister 427 were grown in SDM-79 JRH 57453 media (Life Technologies UK) supplemented with 7.5 mg/l of hemin (Sigma-Aldrich ...
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bioRxiv - Genetics 2023Quote: ... Drosophila MAGs were prepared by dissecting accessory glands from 50 fly abdomens (Canton-S strain) into 50 ul of PBS (Gibco, pH 7.2) and homogenized with a pellet pestle motor (Kimble) ...
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bioRxiv - Biochemistry 2023Quote: ... Digested scFv inserts were subcloned in pET22b(+) vector (EMD Biosciences) and transformed into BL21(DE3) E-coli strain (Thermo Fisher Scientific, USA). Amber stop codon in CDR regions of scFvs were mutated to glutamine by site-directed mutagenesis kit (Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2022Quote: ... aureus (200 ng/mL each) were added into the mixed culture containing three DNA sensor strains in separate 14 mL Falcon tubes (Thermo Fisher Scientific). The DNA sensor strains were incubated at 37°C with shaking (250 rpm ...
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bioRxiv - Genomics 2023Quote: ... Genomic DNA samples used for loading comprised around 50–150 ng per strain (measured by Qubit 4 Fluorometer; ThermoFisher Scientific, Waltham, USA). The sequencing ran for 48 hrs using the MinKNOW software versions 2.2 ...
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bioRxiv - Plant Biology 2023Quote: ... Triplicates of 200 uL for each strain cell culture were transferred into Nunc 96-well black/clear bottom plates (Thermo Scientific, 165305). mNG detection was performed using Ex ...
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bioRxiv - Microbiology 2023Quote: ... A 10 µl volume of phage lysate was added to a log-phase culture of the Klebsiella host strain in LB supplemented with 5 mM CaCl2 (Thermo Fisher Scientific) and 5 mM MgCl2 (Thermo Fisher Scientific ...