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Citations for Thermo Fisher :
5101 - 5150 of 7943 citations for Recombinant Human Leptin Receptor His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Human foreskin fibroblast (HFF-1) cells (ATCC SCRC-1041) were maintained in DMEM (Gibco) with 20% FBS ...
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bioRxiv - Microbiology 2024Quote: Human Foreskin Fibroblasts (HFFs) were maintained in Dulbecco’s minimal essential medium (DMEM; Fisher Scientific) and BS-C-1 cells (ATCC CCL-26 ...
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bioRxiv - Developmental Biology 2024Quote: ... Human iPSCs were cultured in feeder-free conditions on GelTrex-coated plates (ThermoFisher Scientific) in mTeSR-1 media (Stem Cell Technologies ...
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bioRxiv - Neuroscience 2024Quote: The human DBT cDNA plasmid was obtained from the Ultimate ORF collection (Thermo Fisher). Synonymous mutations in the DBT cDNA were generated with Q5-site direct mutagenesis (NEB ...
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bioRxiv - Cell Biology 2024Quote: ... Alexa Fluor 488- and Alexa Fluor 594-conjugated human transferrin were obtained from Invitrogen. Brefeldin A (BFA) ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were quantified by an ELISA kit for total human tau (ThermoFisher Cat# KHB0041) as per manufacturer’s protocol to determine brain ISF tau levels before ...
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bioRxiv - Molecular Biology 2024Quote: ... RRID:CVCL_0291) and Flp-In-T-REx-293 (human, female, embryonic kidney, epithelial; Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... were cultured as standard in Human Large Vessel Endothelial Cell Basal Medium (Gibco, M200500) supplemented with low serum growth supplement (Gibco ...
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bioRxiv - Systems Biology 2024Quote: ... The resulting suspension of human tumor cells was adjusted in DMEM medium (Gibco, #11965084) to a concentration appropriate for loading into a 10X genomics Chromium controller (3’ gene expression kit ...
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bioRxiv - Immunology 2024Quote: ... Isolated T cells were immediately activated with DynaBeads Human T-Activator CD3/CD28 (ThermoFisher), using a 1:1 bead:cell ratio ...
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bioRxiv - Physiology 2024Quote: ... Human THP-1 monocytes were cultured in RPMI 1640 medium (Gibco, cat no. 11875093) supplemented with 10% FCS ...
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bioRxiv - Immunology 2024Quote: ... an Alexa Fluor 647-labeled goat anti-human IgG [H+L] secondary antibody (Invitrogen) was used to detect bound IgG1 to the bacterial surface ...
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bioRxiv - Cell Biology 2020Quote: ... Clarified lysates were then incubated with 20 μg of recombinant glutathione S-transferase (GST)-RBD-MICAL-L1 and Pierce™ Glutathione magnetic agarose beads (Life Technologies-Invitrogen, cat# 78601) for 2 hrs at 4°C ...
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bioRxiv - Immunology 2021Quote: ... were transfected to HEK293 cells (ATCC® CRL-1573™) and each recombinant protein was purified using HisPur™ Ni-NTA column (Invitrogen, Cat# 88226). The specificity of recombinant protein was further validated by SDS-PAGE and Western blot evaluations.
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bioRxiv - Immunology 2024Quote: All CoMiX were generated from HEK293T cells transfected with four μg of recombinant plasmid DNA using Lipofectamine-3000 (Invitrogen, Thermo Fisher Scientific BVBA, Merelbeke, Belgium) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: The VHH M13 phage-display library was subjected to two rounds of affinity enrichment (panning) against immobilized recombinant OspA on Nunc-Immunotubes™ (Thermo Fisher Scientific, Waltham, MA) using protocols described elsewhere (56) ...
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bioRxiv - Biochemistry 2023Quote: ... and the bound target-Fab complexes were incubated with an HRP-conjugated Pierce recombinant protein L (cat: 32420, Thermofisher, 1:5000 dilution in ELISA buffer) for 30 min ...
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bioRxiv - Molecular Biology 2023Quote: ... this latter was performed on 1 ng of genomic DNA (gDNA) of each organism listen in Table 2 by using the Taq DNA polymerase recombinant (Invitrogen™, Carlsbad, 254 CA, USA) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... with formulations calling for L-WRN conditioned medium instead using 500 pM Gibco™ Wnt Surrogate-Fc Fusion Recombinant Protein (Thermo Fisher Scientific, cat# PHG0402), 500 ng/mL recombinant RSPO1 (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... platform and 80 samples were analyzed using GPL570 (Affymetrix Human Genome U133 Plus 2.0 Array) platform ...
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bioRxiv - Cancer Biology 2021Quote: ... anti-human CD117 known also as cKIT (12-1178-42, Thermo Fisher Scientific, 1:100).
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bioRxiv - Cell Biology 2020Quote: ... Human iPSC lines were maintained in 5% CO2 environment in Essential 8 Medium (Thermo Fisher). For cardiomyocyte differentiation ...
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bioRxiv - Immunology 2021Quote: The IL-2 ELISA was performed using the IL-2 Human ELISA Kit by ThermoFisher scientific according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2020Quote: ... T cells were stimulated for 2 days with anti-human CD3/CD28 magnetic Dynabeads (ThermoFisher) at a bead to cell concentration of 1:1 ...
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bioRxiv - Molecular Biology 2022Quote: ... Primary human and mouse fibroblasts were cultured in Minimum Essential Media (MEM, Thermo Fisher Scientific) supplemented with 15% FBS ...
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bioRxiv - Microbiology 2022Quote: ... Epidermal sheets were cultured separately in RPMI with 10% human AB serum (Thermo Fisher Scientific) and 1% Penicillin/Streptomycin/Amphotericin B solution for 48 h ...
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bioRxiv - Neuroscience 2022Quote: Human α-syn fibrils were labelled with Alexa-488 succinimidyl esther (Thermo Fisher Scientific, A20000) following manufacturer’s instructions and the unbound fluorophore was removed with multiple dialysis steps in sterile PBS ...
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bioRxiv - Neuroscience 2019Quote: ... human iPSC-derived astrocytes were incubated with the calcium indicator Fluo-4 AM (1μM; ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... human fetal lung tissues were dissociated with Dispase (8 U/ml Thermo Fisher Scientific, 17105041) at room temperature (RT ...
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bioRxiv - Immunology 2021Quote: ... Anti-human TIGIT PE Cy 7 (1:50, clone MBSA43, Invitrogen, cat # 25-9500-42), Anti-human CD94 APC (1:100 ...
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bioRxiv - Immunology 2021Quote: ... Secreted IL-1β was measured with the Human IL-1β cytoset ELISA (Invitrogen; Cat.# CHC1213) as per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: Human embryonic kidney (HEK) 293T cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco) and supplemented with 10% fetal bovine serum ...
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bioRxiv - Immunology 2019Quote: ... and then stained with Alexa 647-conjugated anti-human IgG (H+L) antibody (Thermo Fisher) for 30 min at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: The human keratinocytes N/TERT-1 cells (27) were cultured in keratinocyte SFM media (Thermofisher) at 37°C under 5% CO2 atmosphere ...
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bioRxiv - Immunology 2019Quote: ... and human/mouse TGF beta 1 ELISA Ready-SET-Go! Kit (2nd Generation; Affymetrix, eBioscience), respectively ...
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bioRxiv - Cell Biology 2019Quote: ... GSE41258 was generated using the GPL96 platform for transcriptome analysis (Affymetrix Human Genome U133A array), while the other datasets were generated using the GPL570 platform (Affymetrix Human Genome U133Plus 2.0 arrays) ...
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bioRxiv - Cell Biology 2019Quote: The human dynein complex was expressed and purified from insect cells (ExpiSf9 cells; Life Technologies) as previously described with minor modifications72 ...
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bioRxiv - Systems Biology 2019Quote: ... Biotinylated probes were hybridized to GeneChip Human Gene 1.0 ST Arrays (Affymetrix, Santa Clara, CA) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... human foetal lung tissues were dissociated using Dispase (8 U/ml Thermo Fisher Scientific, 17105041) at room temperature (RT ...
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bioRxiv - Cell Biology 2020Quote: ... was obtained from Abcam (Cat.# ab9498) (Cambridge, MA, USA) and anti-human ZO-1 antibody was obtained from Invitrogen / ThermoFisher (Carlsbad ...
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bioRxiv - Developmental Biology 2021Quote: ... Mouse and human sperm were loaded with Cal-520-AM (5 μM, Molecular Probes, USA) in the presence of Pluronic F-127 (0.02% v/v ...
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bioRxiv - Immunology 2021Quote: ... human cGAS was labelled with AlexaFluor-488 (AF488) carboxylic acid (succinimidyl ester) (Thermo Fisher Scientific) according to manufacturer’s manuals using a molar ratio of 1:10 at 4°C for 4 h ...
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bioRxiv - Cancer Biology 2020Quote: Lentiviral particles were produced in human 293T cultured in in Dulbecco’s Modified Eagle’s Medium (ThermoFisher), supplemented with 10% Fetal Bovine Serum (EuroBio ...
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bioRxiv - Bioengineering 2021Quote: ... We then stimulated T cells with Dynabeads™ Human T-Activator CD3/CD28 from ThermoFisher overnight with a ratio of beads to cells as 1:3 ...
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bioRxiv - Bioengineering 2020Quote: ... Human cell lines PC-3 (ATCC® CRL-1435™) were grown in RPMI1640 (Gibco) supplemented with 10% (v/v ...
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bioRxiv - Neuroscience 2020Quote: Relative quantitation of secreted Aβ40 was performed with commercial human Aβ40 ELISA kit (Invitrogen, #KHB3481) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... The Silencer™ Select Pre-Designed siRNA for human BAHD1 (Thermo Fisher 4392422, siRNA # s22604) was ordered and used per vendor’s guideline.
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bioRxiv - Cell Biology 2021Quote: ... human chimeric B2 were digested with immobilized papain using Pierce™ Fab Preparation kit (ThermoFisher) following the instructions.
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bioRxiv - Cell Biology 2021Quote: ... Bound B2-IgG was stained with goat anti-human IgG-Alexa 568 (1:1,000; Invitrogen) for 30 min and analyzed by FACS ...
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bioRxiv - Cell Biology 2021Quote: ... Human colon carcinoma HCT116 cells were cultured in McCoy’s 5A Modified Medium (Gibco #16600-082) supplemented with 10% (v/v ...