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Citations for Thermo Fisher :
5101 - 5150 of 10000+ citations for Coatomer Subunit Epsilon COPE Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... Antibody-stained larvae were then transferred to a 1:250 secondary antibody cocktail containing an AlexaFluor Plus 488 goat anti-mouse secondary antibody (Thermo Fisher Scientific, #A-11001) in PBDT with 5% goat serum ...
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bioRxiv - Neuroscience 2024Quote: ... samples were washed 3x 5min with PBST and counter stained for 1h with nuclear stain (Hoechst 33258) and secondary antibodies: rabbit antibodies - goat anti-rabbit conjugated with Alexa Fluor 647 (ThermoFisher, A27040, 1/1000 dilution); goat antibody - rabbit anti-goat conjugated with Alexa Fluor 647 (ThermoFisher ...
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bioRxiv - Biophysics 2023Quote: ... Total tubulin was labeled using a primary antibody against alpha-tubulin and a Cy3-tagged secondary antibody (Thermo Fisher Scientific Inc., Waltham, MA, USA).
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bioRxiv - Cell Biology 2023Quote: ... and blotted with anti-6xHis antibody (1:5000 dilution) followed by HRP-labeled secondary antibody and ECL reagents from the Pierce Fast Western kit (Thermo Scientific, Waltham, MA, USA). The bottom half was stained with SimplyBlue Safe stain (Life Technology ...
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bioRxiv - Microbiology 2023Quote: ... and primary antibody staining was carried out overnight at 4°C (polyclonal anti-PBP2a, custom antibody from Thermo Fisher, 1:1000 or polyclonal anti-Sortase A, custom antibody from Thermo Fisher 1:1000). Secondary antibody staining was performed using an anti-rabbit antibody (Azure anti-rabbit NIR700 or Azure anti-rabbit NIR800 at 1:20000 dilution) ...
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bioRxiv - Neuroscience 2023Quote: ... we incubated the tissue with the primary antibodies against ChAT and PV described above with a blue secondary antibody (donkey anti-rabbit AF350, Cat#A10039, Thermo Fisher Scientific; 1:200) or (donkey anti-sheep AF350 ...
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bioRxiv - Neuroscience 2023Quote: ... The following antibodies were diluted in 1 % non-fat dry milk in TBS-T: mouse anti-TFR1 antibody (1:1,000, Thermo Fisher Scientific, 13-6800, RRID:AB_2533029), rabbit anti-FPN antibody (1:2,000 ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were incubated with the following secondary antibodies diluted 1/200 : Goat anti-Mouse IgG secondary antibody (Alexa 488, Invitrogen-Molecular Probes : A-A11001), Goat anti-Mouse IgG secondary antibody (Alexa 568 ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed 3 times with IF buffer and incubated with the following secondary antibody: Goat anti-Rabbit IgG secondary antibody (Alexa 568, Invitrogen-Molecular Probes : A-11011) for 1 hour at room temperature in the dark ...
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bioRxiv - Neuroscience 2022Quote: ... A subset of sections from brains with FLEX-SomArchon expression were stained with a guinea pig anti-PV antibody (GP72, Swant, 1:1000) following by Alexa Fluor 568 goat anti-guinea pig secondary antibody (No: A-11075, Thermo Fisher Scientific, 1:500). All antibodies were diluted in 2% normal goat serum and 0.5% Triton-X (No ...
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bioRxiv - Biophysics 2022Quote: ... The acini were then rinsed with PBS and incubated with secondary antibody Alexa Fluor 647 goat anti-mouse antibody (Invitrogen, working dilution 1:200), or Alexa Fluor 488 donkey anti-mouse antibody ...
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bioRxiv - Bioengineering 2022Quote: ... The other half was stained with the type II collagen antibody (prior to fixing with 4% PFA) by incubating the primary antibody (Invitrogen, MA5-13026, 1:100) for 2 hours followed by fixing for 30 minutes and a secondary stain for 2 hours (Abcam ...
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bioRxiv - Neuroscience 2023Quote: Sections were washed in PBS three times for 5 min each and incubated in secondary antibodies in PBS (goat or donkey anti-rabbit, anti-mouse, or anti-rat fluorescent antibodies; Invitrogen Alexa Fluor; 1:500) for 1 h at room temperature in the dark ...
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bioRxiv - Neuroscience 2022Quote: ... Then slices were treated with blocking buffer (PBT with 10% donkey serum) for 30 minutes and then underwent incubation by anti-GFP antibody (GFP Polyclonal Antibody, Alexa Fluor™ 488, Invitrogen A21311, Lot #2017366) in PBT with 1% donkey serum at 4℃for 12-24 hours ...
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bioRxiv - Neuroscience 2022Quote: ... Detection of primary antibodies was performed with the following secondary antibodies that were visualized with a LI-COR Odyssey imager (Thermo Fisher; RRID:AB_1965956 and RRID:AB_2556622). GNE-3511 and JNK inhibitor VIII were from Cayman Chemicals.
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bioRxiv - Neuroscience 2023Quote: ... for 2 h RT and incubated o/n with a primary antibody cocktail containing anti-GFP antibody (polyclonal chicken anti-GFP, 1:2000; #A10262, AB_2534023; Thermo Fisher Scientific, Rockford, IL, USA), anti-TH antibody (TH polyclonal rabbit anti-mouse ...
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bioRxiv - Biochemistry 2023Quote: ... the mice bodies were perfused in the immunostaining buffer at room temperature with the Alexa fluorescent dye-conjugated secondary antibodies: Alexa Fluor 647 goat anti-rabbit IgG antibody (Thermo Fisher Scientific, A-21245) or Alexa Fluor 647 goat anti-rat IgG antibody (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... or detected with a monoclonal mouse antibody (SantaCruz, sc-245, 1:250) and visualised with a donkey anti-mouse Alexa Fluor 647 secondary antibody (Molecular Probes, A31571, 1:1000). SCC1/ RAD21 was detected with a monoclonal mouse antibody (Millipore ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... NK-CD16 cells with positive SmBiT expression were selected via cell sorting by probing HA tag (anti-HA antibody [Invitrogen #26183, 1:500]; Anti-mouse secondary antibody Alexa Fluor 647 [Invitrogen #A-21235, 1:200]), probing CD16 (anti-CD16 antibody [Abcam #246222 ...
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bioRxiv - Neuroscience 2023Quote: ... The blot was probed with the antibody and an HRP conjugated donkey polyclonal anti-mouse IgG secondary antibody (PA1-28664; Invitrogen; Lot # WI3375112, Clone: polyclonal) and a band was observed between 50-37 kDa ...
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bioRxiv - Neuroscience 2023Quote: ... The blot was probed with the antibody and an HRP conjugated donkey polyclonal anti-mouse IgG secondary antibody (PA1-28664; Invitrogen; Lot # WI3375112, Clone: polyclonal) and doublet bands were observed between 75-50 kDa ...
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bioRxiv - Neuroscience 2023Quote: ... The blot was probed with the unconjugated parental antibody and an HRP conjugated donkey polyclonal anti-mouse IgG secondary antibody (PA1-28664; Invitrogen; Lot # WI3375112, Clone: polyclonal) and a band was observed between 100-150 kDa ...
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bioRxiv - Neuroscience 2023Quote: ... The blot was probed with the antibody and an HRP conjugated donkey polyclonal anti-mouse IgG secondary antibody (PA1-28664; Invitrogen; Lot # WI3375112, Clone: polyclonal) and a band was observed between 250-150 kDa ...
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bioRxiv - Neuroscience 2023Quote: ... The blot was probed with the antibody and an HRP conjugated donkey polyclonal anti-mouse IgG secondary antibody (PA1-28664; Invitrogen; Lot # WI3375112, Clone: polyclonal) and a band was observed at between 20 kDa-15 kDa ...
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bioRxiv - Neuroscience 2023Quote: ... The blots were probed with the antibody and an HRP conjugated donkey polyclonal anti-goat IgG secondary antibody (PA1-28664; Invitrogen; Lot # WI3375112, Clone: polyclonal) and a single band was observed between 100 kDa-75 kDa ...
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bioRxiv - Microbiology 2023Quote: ... Secondary antibodies used were Alexa 488- and 594- conjugated donkey α-mouse or α-rabbit IgG antibodies (Invitrogen Life Technologies; #A-21202, #A-21207), and goat α-mouse or α-rabbit IgG AP-conjugate antibodies (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2023Quote: ... Secondary antibodies used were Alexa 488- and 594- conjugated donkey α-mouse or α-rabbit IgG antibodies (Invitrogen Life Technologies; #A-21202, #A-21207), and goat α-mouse or α-rabbit IgG AP-conjugate antibodies (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2023Quote: ... Alexa Fluor™ 488-tagged anti-SUMO2/3 antibody was labelled using an Alexa Fluor® 488 Antibody Labeling Kit (Invitrogen-ThermoFisher, Waltham, MA). Unless otherwise specified ...
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bioRxiv - Cell Biology 2023Quote: ... Alexa Fluor™ 488-tagged anti-SUMO2/3 antibody was labelled using an Alexa Fluor® 488 Antibody Labeling Kit (Invitrogen-ThermoFisher, Waltham, MA). Unless otherwise specified ...
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bioRxiv - Developmental Biology 2024Quote: ... CMs were fixed with 4% paraformaldehyde and stained with Ki67 or p-H3 antibody followed by Alexa 594-conjugated secondary antibody (Thermo Fisher Scientific, Waltham, MA). The immunostaining was visualized and imaged on a Keyence BZ-X800 microscope ...
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bioRxiv - Developmental Biology 2024Quote: ... Primary antibodies (Major Resources Table) were incubated at 4°C overnight and secondary antibodies (Alexa 488 or Alexa 555, Thermo Fisher Scientific, Waltham, MA) were incubated at room temperature for one hour ...
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bioRxiv - Molecular Biology 2024Quote: ... The primary and the secondary antibodies were anti-Squ antibody (in this study, 1:500) and Alexa Fluor 488-conjugated anti-rat IgG (Thermo Fisher Scientific, 1:200). Images were taken by ZEISS LSM 900 with Airy Scan 2 using 63X oil NA 1.4 objectives and processed by ZEISS ZEN 3.0 and ImageJ48.
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bioRxiv - Neuroscience 2024Quote: ... and incubated for 2 hours with secondary antibodies diluted in blocking buffer (secondary antibodies: AlexaFluor 555-goat anti Rabbit IgG, (ThermoFisher A-21428, 1:500), AlexaFluor 647 goat anti Rat IgG(ThermoFisher A-21247 ...
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bioRxiv - Microbiology 2024Quote: ... followed by a secondary antibody directed against mouse antibodies coupled to the Alexa-488 fluorochrome (1/1000, A-11001, Invitrogen by Life Technologies, Thermo Fisher Scientific, France). Primary antibodies were incubated for 2.5 hours at 37°C ...
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Single-nucleus multi-omics identifies shared and distinct pathways in Pick’s and Alzheimer’s diseasebioRxiv - Neuroscience 2024Quote: ... at 4°C overnight (IBA1 antibody; Cat #NC9288364; 1:1000; Fisher Scientific, GFAP Polyclonal Antibody; Cat #PA3-16727; 1:500; ThermoFisher, p-tau (AT8) Cat #MN1020 ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were rewashed with PBS and incubated with the corresponding secondary antibodies: (i) AlexaFluor 488-conjugated secondary anti-rabbit antibody (Invitrogen, Cat. No. A-11008) used at 1:200 for GFAP and IBA-1 (2h at 37°C) ...
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bioRxiv - Microbiology 2024Quote: ... cells were probed with Alexa Fluor 568-conjugated goat anti-rat antibody or 488-conjugated goat anti-rabbit antibody (Invitrogen-Molecular Probes, Paisley, U.K.), diluted 1:200 or 1:1000 in PBS ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were exposed to a secondary antibody solution containing the secondary antibody of interest (1:500; #ab150173, abcam; #A21206, Invitrogen; #A31572, Invitrogen; #A21207, Thermo Fisher Scientific) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated with primary antibody (see Table S7) in blocking solution overnight at 4°C and for one hour with secondary antibodies Alexafluor (Thermo Fisher Scientific, Waltham MA) at a concentration of 1:1000.
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bioRxiv - Biochemistry 2024Quote: ... Cells were then chilled on ice and stained with monoclonal anti-TfR antibodies (DSHB, #G1/221/12) at a final concentration of 0.1 μg/mL and APC-conjugated secondary antibodies (Thermo Fisher Scientific, #17-4015- 82) at a final concentration of 0.8 μg/mL 53 ...
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bioRxiv - Biophysics 2024Quote: ... Samples were rinsed three times with PBS and then exchanged with the secondary antibodies mixture comprising antibody goat anti-mouse IgG (H+L) Alexa Fluor 546 (1:400, 10002502, Fisher Scientific GmbH, Schwerte, Germany) and goat anti-rabbit IgG (H+L ...
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bioRxiv - Bioengineering 2024Quote: ... PCs and ACs were stained with neural/glial antigen 2 (NG-2) antibody (eBioscience, 14-6504-80, 1:100) and glial fibrillary acidic protein (GFAP) antibody (Invitrogen, PA1-10019, 1:1000), respectively ...
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bioRxiv - Cell Biology 2024Quote: ... Primary antibodies were recognized by secondary antibodies either anti-mouse or anti-rabbit IgG HRP-conjugated diluted 1/10000 (Thermo Fisher A11005 and A31556). Blots were developed using the ECL plus western blotting system (GE Healthcare ...
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bioRxiv - Cancer Biology 2024Quote: ... the cells were washed three times with PBS and incubated for 1 h at room temperature with the secondary antibodies: donkey anti-mouse Alexa Fluor 488 antibody (3553, ThermoFisher Scientific, 1:500 dilution) and/or goat anti-rabbit Alexa Fluor 568 antibody (A11036 ...
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bioRxiv - Cell Biology 2024Quote: ... followed by confirmation of antibody recognition a protein of the correct molecular weight that was also recognized by α-V5 antibody (Invitrogen; R96025; 1:5000) or α-myc antibody (Cell Signaling Technology ...
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bioRxiv - Plant Biology 2020Quote: ... and Alexa Fluor 555-labeled anti-rabbit antibody (#A20739: Invitrogen). The secondary antibody solution was diluted 200 times with the same blocking buffer used in the primary reaction ...
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bioRxiv - Plant Biology 2020Quote: ... Secondary antibodies used were always HRP-conjugated anti-rabbit (Invitrogen). HRP-peroxidase chemiluminescent substrate (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... All antibodies were also incubated with Dynabeads Protein G (Invitrogen) that served as reference binding for all antibodies ...
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bioRxiv - Molecular Biology 2020Quote: ... using ChIP-grade antibody against V5 (Thermo Fisher Scientific, R960CUS), IgG (Cell signaling technology ...
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bioRxiv - Cancer Biology 2021Quote: ... Compensation was performed using single antibody stained OneComp eBeads (ThermoFisher). Total cell number was determined by multiplying the percentage of live fluorophore positive cells by the total number of live cells isolated per sample and this was normalised to tissue weight.