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Citations for Thermo Fisher :
5051 - 5100 of 5196 citations for Tryptase gamma Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... the fractionated peptides spiked with iRT were loaded onto a pre-column (3 μm, 100 Å, 20 mm × 75 mm i.d., Thermo Fisher Scientific, USA) using a Thermo Scientific UltiMateTM 3000 RSLCnano LC a U3000 LC system ...
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bioRxiv - Plant Biology 2024Quote: ... The resulting peptides were desalted and subsequently analysed on UltiMate™ 3000 RSLCnano System connected to LTQ Orbitrap Velos mass spectrometer (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2024Quote: ... The Eluting peptides from the column were analyzed on a quadrupole-ion trap-Orbitrap QExactive Plus mass spectrometer (Thermo Scientific, San Jose, CA). Orbitrap survey MS1 scans from 375 m/z to 1500 m/z were performed at a resolving power of 70,000 at 200 m/z with an AGC target of 3×106 ions and maximum injection time set to 100 ms ...
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bioRxiv - Molecular Biology 2024Quote: ... Peptides were analyzed by liquid chromatography–tandem mass spectrometry (LC-MS/MS) on an Orbitrap Fusion Lumos mass spectrometer (ThermoFisher Scientific, MA, USA) 54 ...
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bioRxiv - Biochemistry 2024Quote: ... 1 μg of peptide from each sample was taken and analyzed by mass spectrometry using an EASY-nLC1200 chromatography system (Thermo Fisher Scientific, USA) and an Orbitrap Fusion Lumos Tribrid mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... A total of 1 µg of peptides was loaded onto the Acclaim PepMap nano-trap column (C18, 100 Å, 75 μm × 2 cm, Thermo Fisher Scientific) with an isocratic flow of 6 µL/min of 2% ACN containing 0.05% TFA for 6 min ...
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bioRxiv - Cell Biology 2024Quote: ... Peptides were trapped on a C18 Acclaim PepMap 100 (5 μm, 300 μm x 5 mm) trap column (ThermoFisher Scientific, San Jose, USA) and eluted onto a C18 Acclaim PepMap100 3 μm ...
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bioRxiv - Synthetic Biology 2024Quote: ... Peptides were then desalted using SDB-XC Empore disk membranes in StageTips (GL Sciences, Tokyo, Japan) and measured with the Pierce™ Quantitative Colorimetric Peptide Assay (Thermo Fisher Scientific). Equal quantities of purified peptides from each sample were then dried and prepared for TMT labeling ...
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bioRxiv - Synthetic Biology 2024Quote: ... Peptide analysis was conducted using an Orbitrap Fusion Lumos Tribrid mass spectrometer (quadrupole-ion trap-Orbitrap, Thermo Fisher Scientific, San Jose, CA, USA), coupled with an Ultimate 3000 nanoLC system (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... and 3 µg peptides of each sample was injected for LC-MS/MS analysis on an Ultimate 3000 RSLC nanoLC (Thermo Scientific, Bremen, Germany) in-line connected to an LTQ-Orbitrap Elite (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... Then embryos were incubated in the blocking solution for at least 4 hours and incubated overnight at 4°C with secondary antibodies anti-chicken-AF488 (Invitrogen, A11039, used at 1/800), anti-rabbit-HRP (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Coverslips were incubated in blocking buffer with fluorescent secondary antibodies (Donkey α-Rabbit 488 or Donkey α-Rabbit 546, ThermoFisher A-21206 or A-10040) for 4 hours at room temperature in the dark without agitation ...
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bioRxiv - Cancer Biology 2022Quote: ... and stained with secondary antibodies (donkey anti-rat-AF488 [A212008, Invitrogen] and donkey anti-goat AF647 [A21447, Invitrogen] diluted at 1:400 in blocking buffer) for 2 days at 4°C with shaking ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed with phosphate buffered Tween (PBS-T) and non-specific binding was blocked with TBS Startblock blocking buffer (Thermo Fisher Scientific, Rochester, NY, USA). Serum samples were serially diluted 3-fold starting with a 1:100 dilution and BAL and nasal wash samples were serially diluted 2-fold starting with a 1:2 dilution ...
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bioRxiv - Neuroscience 2019Quote: ... the neurons were incubated with goat anti-rabbit IgG antibody conjugated with Alexa Fluor 488 dye (A-11070, Life Technologies; 1000x dilution in blocking solution), and anti-mouse IgG antibody conjugated with Alexa Fluor 568 dye (A-11019 ...
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Evolutionarily divergent mTOR remodels the translatome to drive rapid wound closure and regenerationbioRxiv - Developmental Biology 2021Quote: ... Slides were washed three times 15 min in blocking buffer and incubated with secondary antibody conjugated with Alexa Fluor 488 or Alexa Fluor 568 (Thermo Fisher, A-11017 or A10037) at 1:500 dilution and DAPI at 1:1000 dilution in blocking buffer for 1h in the dark at RT ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were washed 3 times with blocking solution followed by incubation with Alexa 488-conjugated goat anti-rabbit IgG (H+L) (Life Technologies:# A-11034; 1:5000) for 2.5 h ...
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bioRxiv - Physiology 2019Quote: ... Cells or tissue were rinsed in TX/PBS and incubated in blocking solution containing secondary antibody (Alexa conjugated, Life Technologies, 1:1000, 2 hours, RT). After staining ...
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bioRxiv - Physiology 2021Quote: ... After primary incubations the RBCs were washed three times in PBS and incubated for three hours on a rotator at room temperature in blocking buffer containing secondary antibodies: 1:500 goat anti-mouse (AlexaFlour 568; Thermo Fisher Life Technologies A-11031), 1:500 goat anti-rabbit (AlexaFlour 488 ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were subjected to blocking in 0.1M phosphate buffer with 0.3% Triton X-100 (PBTx) containing 10% horse serum (Thermo Fisher Scientific, Cat. No. 26-050-088) and 0.5% bovine serum albumin (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated for 1.5 hours at RT with the corresponding secondary fluorescent antibodies diluted 1:1000 in blocking solution: goat anti-chicken Alexa FluorTM 568 nm (Invitrogen ref. A-11041, RRID AB_2534098), goat anti-rabbit Alexa FluorTM 647 nm (Invitrogen ref ...
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bioRxiv - Immunology 2024Quote: ... The membranes were placed in an opaque gel box and incubated with blocking buffer (PBST + 10% Fish gelatin (PBS 10X, pH 7.2, Thermo Fisher Scientific, Cat-No. 70-013-032; Biotium 10X Fish Gelatin Blocking Agent, Thermo Fisher Scientific, Cat-No. NC0382999) at room temperature for 1 hour ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... followed by the addition of secondary antibodies in blocking buffer (goat-anti-rabbit-IgG-AlexaFluor488 [ThermoFisher Scientific #A11034] and goat-anti-mouse-IgG-AlexaFluor568 [ThermoFisher Scientific #A11031] both 1:200) and Hoechst 33342 (2 ug/mL from 10 mg/mL stock ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cells were then stained with mouse monoclonal anti-CFTR antibody 570 (Cystic Fibrosis Foundation; 1:200 in above blocking buffer) and rabbit polyclonal anti-ZO-1 antibody (ThermoFisher Scientific #40-2200 1:200) for 2 h at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... with a monoclonal antibody against B5 (19C2, rat, 1:1000; (Schmelz et al. 1994)) followed by an Alexa Fluor 647 anti-rat secondary antibody (Invitrogen; 1:1000 in blocking buffer) prior to permeabilization of the cells with detergent ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were then again transferred to new wells containing the blocking solution plus secondary antibodies (goat anti-mouse Alexa Fluor 594 (Thermo Fisher Scientific Cat. #A-11032) at 1:1000 ...
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bioRxiv - Biochemistry 2024Quote: ... the cells were stained with a monoclonal antibody against B5 (19C2, rat, 1:1000 (Schmelz et al, 1994)) followed by an Alexa Fluor 647 anti-rat secondary antibody (Invitrogen; 1:1000 in blocking buffer). Cells were then permeabilized with 0.1% Triton-X/PBS for 3 min ...
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bioRxiv - Biochemistry 2022Quote: ... peptides were loaded on an Acclaim PepMap 100 μ-precolumn cartridge (5 μm, 100 Å, 300 μm ID x 5 mm, Thermo Fisher Scientific). Then ...
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bioRxiv - Neuroscience 2021Quote: The remainder of the light-labeled samples were mixed with the mixture of heavy labeled spike peptides and applied to High-Select™ Fe-NTA Phosphopeptide Enrichment Kit (Thermo fisher Scientific, U.S.A.) to enrich the phosphorylated peptides ...
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bioRxiv - Plant Biology 2019Quote: ... ~100 pmoles) of the peptide samples were separated online using a Dionex Ulimate 3000 RSLCnano pump and autosampler (Thermo Fisher Scientific, Waltham, MA, USA) and a column packed in-house utilizing ProntoSIL C18AQ ...
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bioRxiv - Microbiology 2019Quote: ... Eluted peptides were analysed by liquid chromatography tandem mass spectrometry (LC-MS/MS) on a Dionex UltiMate 3000 RSLCnano System (Thermo Scientific, Waltham, MA, USA) coupled to an Orbitrap Q Exactive mass spectrometer (Thermo Scientific ...
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bioRxiv - Cancer Biology 2021Quote: Tryptic digestion of proteins from samples were followed by a nanoflow LC/MS-MS analysis of peptides using the quadrupole Orbitrap mass spectrometer (Q Exactive HF-X, Thermo Fisher Scientific, Bremen, Germany) (Kelstrup et al ...
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bioRxiv - Neuroscience 2020Quote: ... LC-MS/MS analyses of the generated peptides were performed on a hybrid dual-pressure linear ion trap/orbitrap mass spectrometer (LTQ Orbitrap Velos Pro, Thermo Scientific, San Jose, CA) equipped with an EASY-nLC Ultra HPLC (Thermo Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... The digested peptides were desalted using Oasis HLB cartridges (Waters, Milford, MA, USA) and then dried by vacuum evaporation (Thermo Fisher Scientific, Bremen, Germany).
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bioRxiv - Microbiology 2020Quote: ... Analytical separation of peptides was done on an Acclaim® PepMap RSLC column (75 µm × 50 cm, C18, 3 µm, 100 Å; Thermo Fisher Scientific) running a water-acetonitrile gradient at a flow rate of 300 nl/min ...
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bioRxiv - Microbiology 2020Quote: ... The eluted peptides were electrosprayed at 2 kV into a dual linear quadrupole ion trap Orbitrap Velos Pro mass spectrometer (MS) (Thermo Fisher, San Jose, CA). The MS was set to switch between a full scan (400 < m/z > 2000 ...
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bioRxiv - Microbiology 2020Quote: The fractionated peptides were separated and analyzed using a Dionex Ultimate 3000 RSLCnano system coupled to Q Exactive tandem mass spectrometry (Thermo Fisher Scientific, MA, USA). Separation was performed on a Dionex EASY-Spray 75 μm × 10 cm column packed with PepMap C18 3 μm ...
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bioRxiv - Microbiology 2020Quote: ... A total of 100 μg protein from each condition was subjected to in-solution trypsin digestion before labelling the resultant tryptic peptides using the TMT-6plex Isobaric Label Reagent Set (Thermo Scientific, Rockford, IL, USA) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... The digested peptides were desalted using Oasis HLB cartridges (Waters, Milford, MA, USA) and then dried by vacuum evaporation (Thermo Fisher Scientific, Bremen, Germany).
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bioRxiv - Evolutionary Biology 2022Quote: A 2-μg aliquot of the peptide mixture was analyzed using a LTQ-Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific, San Jose, CA) coupled to an EASY-nLC 1000 (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2022Quote: ... approximately 1.5 μg of peptide per sample was injected into Ultra-High-Performance Liquid Chromatography (UHPLC) system (Ultimate™ 3000 RSLCnano, (Thermo Fisher Scientific, USA) coupled online with Impact HD™ UHR-QqToF (Bruker Daltonics ...
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bioRxiv - Plant Biology 2022Quote: Coding sequences of mature proteins without signal peptide were amplified with primers listed in Table S1 and cloned into expression vector pETSUMO (Thermo Fisher Scientific, Massachusetts, USA). Correctness of the resulting constructs pETSUMO-ChElp2 and pETSUMO-Vd2LysM were confirmed by DNA sequencing and introduced into E ...
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bioRxiv - Plant Biology 2021Quote: A small fraction of all samples were pooled and fractionated using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Pierce, Thermo Fisher Scientific, San Jose). Briefly ...
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bioRxiv - Neuroscience 2020Quote: ... The digested peptides were desalted using Oasis HLB cartridges (Waters, Milford, MA, USA) and then dried by vacuum evaporation (Thermo Fisher Scientific, Bremen, Germany).
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bioRxiv - Immunology 2020Quote: ... The pool of peptides generated in the degradation assay was assessed by mass spectrometry using a Q-Exactive Orbitrap Mass Spectrometer (Thermo Fisher Scientific, Bremen, Germany) and nano-HPLC (Dionex Ultimate 3000 ...
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bioRxiv - Systems Biology 2022Quote: The resulting peptides were dried to 12 µL and analyzed by nanoLC-MS/MS using a Dionex Ultimate 3000 (Thermo Fisher Scientific, Bremen, Germany) coupled to an Orbitrap Eclipse Tribrid mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... and the QI9 peptide (QYIKWPWYI, residues 1208-1216 of the SARS-CoV-2 spike protein) maintained in RPMI 1640 medium (Thermo Fisher Scientific, Cat# 11875101) containing 10% FBS and 30 U/ml recombinant human IL-2 (Peprotec ...
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bioRxiv - Immunology 2022Quote: ... 2 μg of peptide material of each sample was injected for LC-MS/MS analysis on an Ultimate 3000 RSLC nano-LC (Thermo Fisher Scientific, Bremen, Germany) in-line connected to a Q Exactive HF mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... brain-derived tryptic peptides were separated using a NanoAcquity UHPLC (Waters, Milford, FA) and monitored on a Q-Exactive Plus mass spectrometer (ThermoFisher Scientific, San Jose, CA). Raw data were analyzed using MaxQuant v1.5.2.8 with Thermo Foundation 2.0 for RAW file reading capability17 ...
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bioRxiv - Biochemistry 2020Quote: ... The peptides were collected and desalted on Oasis HLB cartridges (Waters, Milford, MA) and then dried by vacuum evaporation (Thermo Fisher Scientific, Bremen, Germany) and stored at −80°C.