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Citations for Thermo Fisher :
5051 - 5100 of 5129 citations for SPFH1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... followed by a peptide clean-up on SPE spin columns before TMT labeling with the TMTpro™ 16plex Label Reagent Set (Thermo Fisher, Cat# A44520). Peptides from each sample were labeled with a distinct TMTpro™ isobaric tag before mixing ...
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bioRxiv - Bioengineering 2023Quote: ... the peptides were separated and analyzed using a Dionex Ultimate 3000 RSLCnano system coupled to a Q Exactive instrument (Thermo Fisher Scientific, MA, USA). Separation was performed on a Dionex EASY-Spray 75 μm × 10 cm column packed with PepMap C18 3 μm ...
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bioRxiv - Cancer Biology 2024Quote: ... Tryptic peptides extracted from each IP product were separated by reverse-phased nano-flow liquid-chromatography (EASY-nLC II, Thermo Scientific, San Jose, CA) and analyzed on an LTQ/Orbitrap Velos Elite hybrid mass spectrometer (Thermo-Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... The peptide amount of tumor cell samples was estimated using a fluorescence based protein assay (Qubit Protein assay, Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Cancer Biology 2023Quote: ... Eluted peptides were subjected to electrospray ionization and then entered into an LTQ Orbitrap Velos Elite ion-trap mass spectrometer (Thermo Fisher Scientific, Waltham, MA). Peptides were detected ...
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bioRxiv - Cell Biology 2023Quote: Peptide concentrations of whole cell S-trap extracts in 100 mM TEAB were quantified using the Pierce Quantitative Fluorometric Peptide Assay (Thermo Scientific, Waltham, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... each peptide sample (approximately 2 mg) was tapplied to a Pierce High-Select™ TiO2 Phosphopeptide Enrichment Kit (Thermo Fisher Scientific, Cat No: A32993). After preparing spin tips as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... peptides were reconstituted in 0.1% trifluoroacetic acid and fractionated on Sep-Pak® Vac cartridges using methodology and reagents from Pierce™ High pH reversed-phase peptide fractionation kit (8 fractions for global proteomics and 4 for phosphoproteomics skipping every other; Thermo Fisher Cat No: 84868). Samples were run (1/8th of each global and 1/5th of each phosphopeptide fraction ...
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bioRxiv - Cancer Biology 2023Quote: ... the peptides were analyzed by online nanoflow liquid chromatography-tandem mass spectrometry performed on an EASY-nanoLC 1200 system (Thermo Fisher Scientific, MA, USA) connected to a Q Exactive™ Plusmass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... and washed for 3 min at 30 μL/min before peptides were eluted onto a C18 Acclaim™ PepMap™ column (100 Å, 5 μm × 0.75 mm × 150 mm, Thermo Fisher Scientific) at a flow rate of 0.3 μL/min ...
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bioRxiv - Biochemistry 2023Quote: ... as three full experimental technical replicates (n=54) with ∼100 ng of peptides injected for each chromatographic run using a Dionex UltiMate 3000 uHPLC system (Thermo Fisher Scientific, Bremen, Germany). Solvent A was 1% CH3CN in 0.1% (v/v ...
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bioRxiv - Cell Biology 2023Quote: For the identification of the peptides a Dionex Ultimate 3000 RSLCnano System coupled to an Orbitrap Eclipse Tribrid Mass Spectrometer (both Thermo Fisher Scientific, Vienna, Austria) was used.
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bioRxiv - Immunology 2023Quote: The inhibition of kinase activity mediated by phospholipids was determined using the following Z’-LYTE Kinase Assay Kits: Z’-LYTE Kinase Assay Kit - Ser/Thr 6 Peptide (Thermo Fisher Scientific, Cat# PV3179) used to measure AKT activity and the Z’-LYTE Kinase Assay Kit - Ser/Thr 7 Peptide (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... Direct pepscan analysis was undertaken by binding 200 ng of each overlapping peptide to Nunc MaxiSorp 96 well ELISA plates (Thermo Fisher Scientific, Waltham, MA) overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... Peptides were loaded on an Acclaim PepMap 100 μ-precolumn cartridge (5 μm, 100 Å, 300 μm ID x 5 mm, Thermo Fisher Scientific) and separated at 40°C on a PicoTip emitter (noncoated ...
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bioRxiv - Biophysics 2024Quote: ... Degraded ProteaseMAXTM was removed via centrifugation (max. speed, 10 minutes) and the peptide solid phase extracted using 10 µl volume C18 tips (Pierce○R (Thermo Fisher Scientific, Waltham, MA) according to manufacturer protocol ...
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bioRxiv - Neuroscience 2024Quote: ... the peptide mixture was split into two equal aliquots of 1 mg each and 350 fmol of standard AQUA QuantPro peptides (Thermo Fisher Scientific, Waltham, USA) were added to each aliquot before K-ε-GG peptide enrichment ...
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bioRxiv - Synthetic Biology 2024Quote: ... Peptides were loaded onto a C18 Acclaim PepMap NanoLC column (25 cm length, 75 µm inner diameter, Thermo Fisher Scientific, San Jose, CA, USA) packed with 2 µm particles and a 100 Å pore size ...
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bioRxiv - Cell Biology 2022Quote: ... then incubated at room temperature for 1 hour in a dark humid chamber with goat anti- mouse IgG-Alexa488 (Invitrogen, A11001, 1:500 in blocking buffer). Cells were washed 4 more times with blocking buffer ...
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bioRxiv - Cell Biology 2020Quote: ... sections were washed 2 times for 8 minutes in DPBS containing 0.3% Triton X100 and then incubated for 1 hour in blocking solution containing the secondary antibody (1/200, Invitrogen P10994 Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated in secondary antibodies diluted with the blocking solution (1:100 for goat anti-mouse Alexa 633 (Thermo Fisher Scientific #A-21052, RRID: AB_2535719), 1:100 for goat anti-chicken Alexa 488 (Thermo Fisher Scientific #A-11039 ...
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bioRxiv - Immunology 2022Quote: ... The cells were washed four times in PBS and incubated with goat anti-rabbit antibody conjugated with AlexaFluor594 for 1 hour at room temperature (Invitrogen; 1:200 dilution in blocking reagent). 4’,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Neuroscience 2019Quote: ... the cells were incubated with goat anti-rabbit or anti-mouse IgG antibody conjugated with Alexa Fluor 488 dye or Alexa Fluor 568 dye (Life Technologies; 1000x dilution in blocking solution) for 60 min at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Triton-X-100 and 5% (v/v) normal goat sera) for 30 min prior to staining with a rabbit anti-HA polyclonal antibody (Invitrogen, diluted 1:100 in blocking buffer) for 1 h ...
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bioRxiv - Microbiology 2022Quote: ... the cells were washed three times with the blocking buffer and incubated at room temperature with fluorophore-conjugated secondary antibodies (goat anti-mouse IgG Alexa 488, Thermo Fisher, Cat #: A11001, 1:500 dilution) (goat anti-rabbit IgG Alexa 555 ...
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bioRxiv - Cancer Biology 2024Quote: ... the mesenteries were incubated with a secondary antibody consisting of a blocking buffer solution and Alexa Fluor 488 goat anti-rabbit IgG (Thermo Fisher Scientific, A-11008, 1:1000) for 1 hour in a darkroom then washed 3 times with PBS for 10 minutes and mounted on microscopic slides ...
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bioRxiv - Bioengineering 2024Quote: ... Blotting took place with the LI-COR system (LI-COR 927-66003 [TBS-based blocking and diluent buffers] and ThermoFisher 28360 TBS-Tween 20 wash buffer) for imaging on a LI-COR Odyssey instrument ...
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Neuron-astrocyte metabolic coupling facilitates spinal plasticity and maintenance of persistent painbioRxiv - Neuroscience 2022Quote: ... before secondary antibody (Donkey α-Rabbit, Alexa Fluor 488, Invitrogen #A21206, 1:2000; Goat α- mouse, Alexa Fluor 488, Invitrogen #A11001, 1:2000 in blocking buffer) was applied to the sections and incubated for 1 h at RT ...
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bioRxiv - Microbiology 2023Quote: ... Resulting biofilms were first washed with 1x PBS to remove loosely-bound cells and incubated with BSA blocking buffer at room temperature for 0.5 h (Thermo Scientific, 3% BSA is PBS; J61655.AK). Biofilms were then probed with two murine monoclonal antibodies against B-DNA (Anti-dsDNA ...
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bioRxiv - Neuroscience 2023Quote: ... blocked in 3% normal donkey serum/0.25% Triton X100 in PBS for 1 h at room temperature and then incubated overnight at room temperature in blocking solution containing primary antiserum (rabbit anti-GFP, 488-conjugated, Novus #NB600-308, 1:5000; rabbit anti-TH, Thermo Fisher Scientific #OPA1-04050, 1:5000). The next morning ...
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bioRxiv - Cell Biology 2023Quote: ... slides were incubated in the dark for 30 min at room temperature with secondary antibodies diluted 1:800 in blocking buffer: donkey anti-rabbit AlexaFluor 488 (Thermo Fisher Scientific, Catalog No. A-21206) and donkey anti-goat AlexaFluor 555 (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... treated with a 10% fetal bovine serum (FBS)/PBS blocking solution (Fisher bioreagents, catalog #BP9703-100) and stained for eIF5A1/2 polyclonal antibody (Thermo Fisher Sci. catalog #PA529204, 1:200) or SMAD3 (Cell Signaling Technology catalog #9523S ...
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bioRxiv - Bioengineering 2023Quote: ... cells were washed three times at 5 minutes each with DPBS before incubation for 1 hour at room temperature in the dark with secondary antibodies in blocking buffer (goat anti-rabbit IgG (H + L) Cross-Adsorbed Secondary Antibody conjugated to Alexa Fluor 488 (ThermoFisher, 1:200 dilution, Cat # A-11008) or goat anti-mouse IgG1 Cross-Adsorbed Secondary Antibody conjugated to Alexa Fluor 488 (ThermoFisher ...
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bioRxiv - Cell Biology 2023Quote: ... sections were washed two times for 8 min in DPBS containing 0.3% Triton X100 and then incubated for 1 hr in blocking solution containing the secondary antibody (1/200, Invitrogen P10994 Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody ...
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bioRxiv - Developmental Biology 2021Quote: ... peptide samples were purified via Stage tips using punched out cookies of C18 3M™ Empore™ extraction disc material (Fisher Scientific #14-386-2) as matrix ...
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bioRxiv - Cell Biology 2022Quote: Protein identification by LC-MS/MS analysis of peptides was performed using an Orbitrap Fusion Lumos Tribid mass spectrometer (Thermo Fisher Scientific, San Jose, CA) interfaced with an Ultimate 3000 Nano-HPLC apparatus (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2019Quote: Nanoflow LC–MS/MS analysis of tryptic peptides was conducted on a quadrupole Orbitrap mass spectrometer (Q Exactive HF-X, Thermo Fisher Scientific, Rockford, IL, USA) [48] coupled to an EASY nLC 1200 ultra-high-pressure system (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... MS spectra and MS/MS spectra were acquired from eluting peptides ionized with electrospray ionization (ESI) and analyzed in a LTQ Orbitrap Velos hybrid mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA), as described previously (Verberkmoes et al. ...
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bioRxiv - Neuroscience 2020Quote: ... The tryptic peptides were separated and analyzed using a Dionex Ultimate 3000 RSLCnano system coupled to a Q Exactive instrument (Thermo Fisher Scientific, San Jose, CA) as previously described (Gallart-Palau et al. ...
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bioRxiv - Cancer Biology 2021Quote: Peptides from all experiments were separated by nanoscale C18 reverse-phase liquid chromatography using an EASY-nLC II 1200 (Thermo Fisher Scientific, Waltham, MA, USA), using a binary gradient with buffer A (2% acetonitrile ...
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bioRxiv - Molecular Biology 2020Quote: ... The tryptic peptides were loaded at 5 μL/min onto a C18 trap column (Thermo Scientific, 100 µm ID×2 cm, 5 μm, 120 Å). Peptides were separated with PicoFrit columns (New Objective ...
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bioRxiv - Biophysics 2020Quote: ... The eluted peptides were analyzed in a mass spectrometer with an orbitrap mass analyser (Orbitrap Fusion™ mass spectrometer, Thermo Scientific, San Jose, California, USA).
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bioRxiv - Neuroscience 2021Quote: ... Briefly, peptides were loaded onto a Thermo NanoViper trap column (75 µm x 20 mm, 3 µm C18, 100 Å) (Thermo Fisher Scientific, Bremen, Germany) using an Agilent 1260 Infinity nanoLC system (Agilent Technologies ...
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bioRxiv - Microbiology 2021Quote: ... using an EASY-nLC 1200 system (peptides were loaded and separated on a 30 cm long home-made C18 column; Thermo Fisher Scientific, Waltham, MA, USA) coupled to a Q Exactive Plus system (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2021Quote: ... Four μl of the different peptide mixtures (800 ng for yeast peptides and 949 ng for bulks) were analyzed using an Orbitrap Fusion™ Lumos™ Tribrid™ mass spectrometer (Thermo Fisher Scientific).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... these peptides were loaded onto the Xbridge BEH300 C18 column (Waters Milford, MA, USA) for HPLC (UltiMate 3000 UHPLC; Thermo Fisher Scientific, Waltham, MA, USA) separation ...
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bioRxiv - Biochemistry 2021Quote: ... For the FAIMS CV scanning of synthetic peptides and glycopeptides, each sample (Table S1, Supporting Information) was directly injected to the Orbitrap Lumos (Thermo Fisher Scientific, San Jose, CA). The CV was scanned from 0-100 V in 1 V steps ...
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bioRxiv - Cancer Biology 2021Quote: ... The eluted peptides were analyzed in a mass spectrometer with an orbitrap mass analyser (Orbitrap Fusion™ mass spectrometer, Thermo Scientific, San Jose, California, USA).
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bioRxiv - Microbiology 2019Quote: ... Samples were resuspended in 20 µL 0.1 % formic acid (FA) and peptides were quantified by measuring the absorbance at 280 nm using a Nanodrop 1000 device (Thermo Fisher Scientific, Rockford, IL, USA).
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bioRxiv - Immunology 2021Quote: ... with an N-terminal IL-2 signal peptide for secretion and a C-terminal 6×His tag for purification was inserted into pFUSE-vector (Invitrogen, Thermo Fischer Scientific, MA, USA). The human ACE2 was expressed by essentially the same protocol used for the COVID-19 RBD ...