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Citations for Thermo Fisher :
5051 - 5100 of 10000+ citations for Hydrogen Peroxide Fluorescent Detection Kit 2 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... The detection and measurement of SARS-CoV-2 viral load were performed using COVID-19 RT-PCR diagnostic kit with specific Taqman probes (ThermoFisher) of SARS-CoV-2-specific genes encoded by the RNA-dependent RNA polymerase (RdRp/ORF1ab) ...
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bioRxiv - Genetics 2020Quote: ... and then 2 μg was used in to prepare cDNA using a High Capacity cDNA Reverse Transcription Kit (Applied Biosystems). After using a nanodrop to determine the concentration of cDNA in the sample ...
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bioRxiv - Microbiology 2021Quote: ... assay using the OPTI Medical SARS-CoV-2 RT-PCR kit following the manufacturer’s instructions on an ABI 7500 Fast instrument (ThermoFisher Scientific). The OPTI Medical SARS-CoV-2 RT-PCR assay detects two different targets in the gene encoding viral nucleocapsid (N ...
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bioRxiv - Physiology 2020Quote: ... We used 2 µg RNA for reverse transcription with the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, CA, USA). The cDNA was diluted (1:10 ...
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bioRxiv - Developmental Biology 2021Quote: ... We injected pregnant mice intraperitoneally with 100 μL of 5-ethynyl-2’-deoxyuridine (EdU, Click-iT EdU Alexa Fluor 647 Imaging Kit, Invitrogen) at 1mg/mL ...
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bioRxiv - Cell Biology 2021Quote: ... HeLa and BJ 1-2 μg of total cell RNA was reverse transcribed into cDNA using SuperScript VILO cDNA Synthesis Kit (11754050, ThermoFisher). A volume corresponding to 25-50 ng of cDNA was used for each RT-qPCR reaction using Roche LightCycler 480 SYBR Green I Master (04707516001 ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA (2 µg) was reverse-transcribed to cDNA template using RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific, K1621). Q-PCR was analyzed in the Applied Biosystems QuantStudio 3 ...
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bioRxiv - Developmental Biology 2021Quote: ... Approximately 2 μg of RNA from each sample were DNAse treated with the DNA-free™ DNA Removal Kit (Invitrogen) in the presence of RiboLock RNase Inhibitor (Thermo Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... The enrichment phase was performed by using the One-Touch 2 device with the Ion Sequencing 400 Kit according to the manufacturer’s instruction (Life Technologies) (Moitinho et al ...
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bioRxiv - Microbiology 2020Quote: ... One-step RT-qPCR was performed with primers targeting the SARS-CoV-2 N region (BEI) using the EXPRESS One-Step Superscript qRT-PCR Kit (ThermoFisher) on an Applied Biosystems QuantStudio3 instrument ...
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bioRxiv - Immunology 2020Quote: ... 2 and 3 cDNA were amplified by real-time PCR using the SYBR Green PCR kit (Thermo Scientific, Braunschweig, Germany). The primer sequences used for the real-time PCR were listed in Table 1.
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bioRxiv - Physiology 2020Quote: ... 2 μg of RNA was used to prepare cDNA with the High Capacity cDNA Reverse Transcription kit (Applied Biosystems, 4368813) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: 5-ethynyl-2-deoxyuridine (EdU) pulse labeling was performed with the Click-IT EdU imaging kit (Life Technologies; Cat#:C10340) as described in (Perillo et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell viability was measured by MTT assay using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide kit (Thermo-Fisher Scientific M6494). For the isobologram assays ...
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bioRxiv - Genomics 2022Quote: ... DNA quality was examined on a 1% TAE agarose gel and DNA concentration was determined using the dsDNA BR Assay Kit on a Qubit 2 fluorometer (Invitrogen).
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bioRxiv - Plant Biology 2022Quote: ... The purified RNA (2 μg) was used for cDNA synthesis using the RevertAid RT Reverse Transcription Kit (Thermo Scientific, USA) in the presence of 100 μM random hexamers (Thermo Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Fab were generated from monoclonal antibodies using the Pierce Mouse IgG1 Fab and F(ab’)2 Preparation Kit (Thermo Fisher). Antibodies were first digested into Fab in a Zeba Desalt Spin Column (Thermo Fisher ...
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bioRxiv - Neuroscience 2022Quote: ... cDNA was generated from 2 μg RNA in 20 μl reactions using High-Capacity Reverse Transcriptase kit (ABI, Thermo Fisher). Real-time quantitative PCR (RT-qPCR ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 µg total RNA was converted to cDNA using Superscript First-stand Synthesis kit (Thermo Fisher Scientific, Waltham, MA, #11904018). qPCR was performed using TaqMan gene expression Master Mix (Applied Biosystems ...
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bioRxiv - Neuroscience 2024Quote: ... 2 µg of total RNA was used for reverse transcription using the high Capacity cDNA Reverse Transcription Kit (Applied Biosystems) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... Eluted RNA (30 µl) was treated with 2 U of DNase for 30 min using the TURBO DNA-free kit (Invitrogen). RNA was cleaned up using the RNA Clean & Concentrator-25 kit (Zymo ...
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bioRxiv - Immunology 2024Quote: ... naïve CD4+ T cells were enriched from homozygous TCR-transgenic OT-II (CD45.1/.2) donors using the Dynabeads Untouched™ Mouse CD4 Cells kit (Invitrogen). 1 x 105 OT-II cells were transferred intravenously (i.v. ...
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bioRxiv - Biophysics 2024Quote: ... mRNA was transcribed for 2-2.5 h with mMESSAGE mMACHINE kits (ThermoFisher, T7 for pcDNA3, SP6 for pSP64-poly(A)) following supplier’s protocols including DNAse treatment and purified with RNA Clean & Concentrator-5 (Zymo Research ...
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bioRxiv - Molecular Biology 2023Quote: Colonies were scraped from the Agar plates and transferred to liquid growth medium (Luria Broth) and grown at 37C for 2 h before plasmid DNA isolation (Maxiprep kit Invitrogen).
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bioRxiv - Evolutionary Biology 2022Quote: The concentration of the gel excised Round 2 Multiplex-Nested-PCR product was quantified by using the Qubit dsDNA HS Assay kit (Invitrogen) according to manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... 2 µg DNAseI digested RNA were used for reverse transcription using the TaqManTM Reverse Transcription kit (N8080234, Thermo Fisher Scientific) with random hexamer primers ...
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bioRxiv - Neuroscience 2023Quote: ... Gene expression values were then set equal to 2−ΔΔCt as described by the manufacturer of the kit (Applied Biosystems). All PCR primers were synthesized by Invitrogen with PPIA as the housekeeping gene (for primer sequences see Supplementary Table 2).
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bioRxiv - Cell Biology 2023Quote: Cell proliferation was assessed using the 5-Ethynyl-2-deoxyuridine (EdU) incorporation assay (Click-iT EdU Alexa Fluor 488 Imaging Kit, Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... AIW002-2 was reprogrammed from peripheral blood mononuclear cells of a healthy donor with the Cytotune reprogramming kit (Thermo Fisher). 3450 was also generated from peripheral blood mononuclear cells ...
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bioRxiv - Genomics 2023Quote: ... cDNA was synthesized from 1-2 μg total RNA with the Multiscribe High-Capacity cDNA Reverse Transcription Kit (ThermoFisher 4368814).
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bioRxiv - Immunology 2023Quote: ... 2 μg of total RNA was used to synthesise cDNA using the High-Capacity RNA to cDNA Kit (Applied Biosystems). The primer sequences were generated using the primer BLAST software (Basic Local Alignment Search Tool)
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bioRxiv - Developmental Biology 2023Quote: ... The mRNA from plasmid #1 and #2 was transcribed using the mMESSAGE mMACHINE SP6 Transcription Kit (Thermo Fisher Scientific, AM1340) and purified with the NucleoSpin RNA Clean-up XS kit (Macherey-Nagel ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA was prepared according to manufacturer’s instructions from 2 ug total RNA using a high-capacity cDNA reverse transcription kit (Applied Biosystems). qPCR reactions were performed in a 384-well format using GoTaq qPCR Master Mix (Promega ...
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bioRxiv - Genomics 2023Quote: ... 2 μg of total RNA reverse transcribed using High-Capacity cDNA Reverse Transcription kit (Applied Biosystems, USA, Cat No. 4368814) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... cells were stained for 10 min in 0.5 µL/mL calcein-AM and 2 µL/mL ethidium homodimer-1 from the Live/Dead assay kit (L3224, Invitrogen). Then ...
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bioRxiv - Immunology 2023Quote: ... 2% SDS and 10% Glycerol) and protein concentration was measured using Pierce™ BCA Protein Assay kit (Thermo-Fisher Scientific). 20µg of protein was resuspended with SDS-loading dye (50% β-Mercaptoethanol and 0.02% Bromphenolblue) ...
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bioRxiv - Plant Biology 2023Quote: RNA in situ hybridization was conducted using Invitrogen ViewRNA ISH Tissue 1-Plex and 2-Plex Assay kits (ThermoFisher Scientific) using the manufacture’s protocol optimized for Medicago root and nodules sections (Kulikova et al. ...
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bioRxiv - Neuroscience 2023Quote: ... 2 μl of extracellular RNA were used with the TaqMan™ Advanced miRNA cDNA Synthesis Kit (Thermo Fisher Scientific, A28007). The miRNAs with the least expression variability between the 48 sequenced samples were identified using geNorm (Vandesompele et al. ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids encoding heavy and light chains were transfected into Expi293F cells in a 2:1 mass ratio using an ExpiFectamine 293 transfection kit (Gibco). Six days post-transfection ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNA was generated using 2 μg of template RNA and a High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, 4368813). cDNA was diluted 1:100 and qPCR was performed using PowerUp SYBY Green Master Mix (Applied Biosytems ...
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bioRxiv - Immunology 2024Quote: Cell culture supernatants collected at various time intervals from in vitro stimulated T cells of mice or humans were analyzed for IL-2 by ELISA using kits purchased from ThermoFisher Scientific (mouse IL-2 ELISA kit ...
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bioRxiv - Cancer Biology 2024Quote: ... Gene expression values were then set equal to 2−ΔΔCt as described by the manufacturer of the kit (Applied Biosystems). All PCR primers were synthesized by Invitrogen and used with PPIA as housekeeping gene (for primer sequences see Suppl ...
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bioRxiv - Molecular Biology 2024Quote: ... At least 2 μg of purified RNA was reverse-transcribed using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems). Quantitative PCRs were carried out on ABI Prism 7900 HT Fast Real-Time PCR System with Taqman 5′-nuclease probe method and SensiFAST Probe Hi-ROX Mix ...
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bioRxiv - Cancer Biology 2024Quote: ... were mixed with 10 pmol Cas9 protein (Integrated DNA Technologies) in 2 µL of Resuspension Buffer R (Neon Electroporation Kit, Cat# MPK1096, ThermoFisher) and incubated at room temperature for 20 minutes to form the CRISPR RNP complex in vitro ...
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bioRxiv - Immunology 2024Quote: ... Cell culture supernatants were collected at the end of culture and analyzed for IL-2 by ELISA using a kit (cat # 88-7025-76) purchased from Thermofisher Scientific.
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bioRxiv - Biochemistry 2024Quote: ... RNA transcripts of viral and host genes were measured by quantitative PCR using primers listed in Table 2 and Power SYBR Green kit (ThermoFisher), on a QuantStudio7 Pro Real-time PCR System (ThermoFisher).
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bioRxiv - Biochemistry 2024Quote: ... Total RNA (2 µg) was then reverse transcribed using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, catalog no. 4374966), according to manufacturer’s instructions.
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Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: ... ExpiCHO cells were transiently transfected with the vector containing the gene encoding soluble spike glycoprotein ectodomain or CR3022 (using a heavy chain:light chain ratio of 1:2) using the Expifectamine 293 Transfection Kit (ThermoFisher #A29129), following the user manual ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Single-stranded cDNA was synthesized from 2 µg of total RNA using a High-Capacity cDNA Archive Kit (Applied Biosystems) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2022Quote: RNA extractions of swab material were performed in 96-well plates using Mag Max-96 AI/ND Viral RNA Isolation Kit (Applied Biosystems, Foster City, California) following the manufacturer’s instructions ...