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Citations for Thermo Fisher :
5051 - 5100 of 10000+ citations for C Reactive Protein Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Purity and integrity of all proteins were checked and protein concentrations estimated using quantitative densitometry on GelCode Blue-stained protein gels (Thermo Fisher Scientific) containing bovine serum albumin (BSA ...
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bioRxiv - Biochemistry 2022Quote: ... Supernatants were kept and protein concentration was estimated using the micro-BCA Protein Assay Kit (Pierce BCA Protein Assay Kit (Thermo Scientific: 23225)) ...
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bioRxiv - Microbiology 2023Quote: ... Protein concentration was measured using the Pierce 660nm Protein Assay Kit or the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific). For each sample ...
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bioRxiv - Immunology 2021Quote: ... and Fc receptors were blocked with Fc block mAb clone 2.4G2 for 15 minutes at 4°C prior to surface staining for 30 minutes at 4°C with anti-CD4 (clone GK1.5 EF450, Invitrogen) and anti-Vβ3 TCR clone (clone KJ25 ...
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bioRxiv - Microbiology 2021Quote: ... Radiolabeled RNA was incubated 5 min at 90°C with alkaline buffer or 5 min at 37°C with ribonuclease T1 (0.1 U; Ambion) to generate the Alkaline (OH ...
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bioRxiv - Genomics 2020Quote: ... 58°C for 30 sec and 72°C for 1.5 min using using AmpliTaq Gold® Polymerase (Applied Biosystems).
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bioRxiv - Biochemistry 2019Quote: ... the pDyn3 plasmid containing the human dynein genes or the pFastBac plasmid containing full-length Lis1 or dynein monomer was transformed into DH10EmBacY chemically competent cells with heat shock at 42°C for 15 seconds followed by incubation at 37°C for 5 hours in S.O.C media (Thermofisher scientific). The cells were then plated on LB-agar plates containing kanamycin (50 μg/ml) ...
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bioRxiv - Microbiology 2021Quote: ... followed by 40 cycles at 95 °C (15 s) alternating with 60 °C (1 min) (Applied Biosystems, Carlsbad, CA). Gene-specific products were normalized to a group of 5 housekeeping genes (ACTB ...
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bioRxiv - Microbiology 2021Quote: ... and 1 min at 60 °C cycle of 50 °C with a 7500 Real-Time PCR System (Applied Biosystems). The following Taqman primer and internal probes were used:
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bioRxiv - Developmental Biology 2022Quote: ... 5,000 control or 125,000 mitomycin C treated cells were suspended in a 37 °C warmed 20 µL 0.5% low melting point agarose (16520, Invitrogen) droplet at the bottom of the µ-slide 8 well dish ...
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bioRxiv - Bioengineering 2019Quote: ... The montaged image in Figure 5 C ii and 5 C iii were generated by stitching together individual images using MAPS 1.1 software (ThermoFisher).
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bioRxiv - Cell Biology 2021Quote: ... the pDyn3 plasmid containing the human dynein genes or the pFastBac plasmid containing full-length Lis1 was transformed into DH10EmBacY chemically competent cells with heat shock at 42°C for 15 seconds followed by incubation at 37°C for 5 hours in S.O.C media (Thermofisher scientific). The cells were then plated on LB-agar plates containing kanamycin (50 μg/ml) ...
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bioRxiv - Molecular Biology 2022Quote: ... reverse transcription was performed with Superscript III (50°C for 60 min, 70°C for 15 min; Invitrogen 18080085) and a primer binding within the poly-A site of candidate mRNAs (reverse_transcription_rv ...
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bioRxiv - Immunology 2023Quote: ... and Fc receptors were blocked with Fc block mAb clone 2.4G2 for 15 minutes at 4°C prior to surface staining for 30 minutes at 4°C with anti-CD4 (clone GK1.5 EF450, Invitrogen) and anti-Vβ3 TCR clone (clone KJ25 ...
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bioRxiv - Physiology 2024Quote: ... and stored at 4 °C overnight prior to storage at −80 °C following RNAlaterTM best practices (Life Technologies, 2011). Following this ...
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bioRxiv - Immunology 2024Quote: Isolated T cells were re-suspended at 1 × 10^6/mL in 37°C pre-warmed PBS and incubated for 20 min at 37°C with 2μM Cell Trace Violet (CTV) (Invitrogen). Cells were washed in RPMI-Glutamax (10% FCS ...
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bioRxiv - Microbiology 2020Quote: 3 ⨯106 cells were washed with FACS buffer (0.2% BSA, 1 mM in PBS) before blocking with Fc block (Invitrogen) and incubation with eF780 live/dead viability stain (eBioscience ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were then incubated in the blocking/0.5 % BSA solution containing 1:500 of a secondary antibody (goat anti-chicken, Alexa Fluor 488, Invitrogen, # A-11039 ...
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bioRxiv - Neuroscience 2021Quote: ... then washing them with 0.1M PBS and a blocking solution composed of 10% Fetal Bovine Serum (FBS; 10500064, Invitrogen) and 0.1M PBS with Triton X-100 (X100 ...
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bioRxiv - Neuroscience 2019Quote: ... all diluted 1:15,000 or 1:30,000 in blocking buffer containing 0.1% Tween 20 and 0.01% SDS: Alexa 680 donkey anti-rabbit (Life Technologies, A10043), Alexa 680 donkey anti-mouse (Life Technologies ...
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bioRxiv - Neuroscience 2019Quote: ... sections were incubated in blocking solution with goat anti-chicken secondary antibodies (Thermo Fisher, Alexa Fluor 488, A-11039) at 1:1000 for 1 hour at room temperature ...
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bioRxiv - Immunology 2021Quote: ... prior to washing in PBS/Tween (0.05% v/v) and blocking with Blocker Casein in PBS (Thermo Fisher Scientific) for 1 hour at room temperature (RT) ...
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bioRxiv - Neuroscience 2020Quote: ... cells were incubated for 1 hour at room temperature in secondary antibodies (1:500 in blocking solution; Molecular Probes). Before mounting ...
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bioRxiv - Neuroscience 2020Quote: ... the sections were incubated three times (5 min each) in permeabilization solution (0.3 % Triton X-100) before blocking for 2 h either with Normal Goat Serum (Invitrogen) in Tris-NaCl blocking buffer (1:5 ...
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bioRxiv - Biophysics 2021Quote: ... Embryos were then washed in blocking solution overnight and incubated with an anti-rabbit Alexa 647 secondary antibody (ThermoFisher) using a 1:250 dilution ...
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bioRxiv - Microbiology 2020Quote: ... Cells were then rinsed for 5 min at RT (3X) with PBS and incubated with secondary antibody (Alexa Fluor 647 diluted 1:2,000 in blocking buffer; Invitrogen) for 45 min at RT ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was subsequently incubated in blocking buffer (0.2% I-Block™, 0.1% Tween) (Thermo Fisher Scientific, MA, USA) at room temperature for 2 h ...
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bioRxiv - Neuroscience 2022Quote: ... Blots were washed successively with blocking buffer, TBST (50mM Tris-Cl, 150mM NaCl, 0.1% Tween™20 (Thermo Fisher)) ...
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bioRxiv - Neuroscience 2022Quote: ... After washing with the blocking solution and PBS the slices were mounted on Superfrost Plus Slides (Thermo Fisher Scientific) and sealed with a Vectashield (VE-H-1000 - Vector Labs ...
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bioRxiv - Microbiology 2022Quote: ... Non-specific staining was minimized by pre-incubating cells with 0.5 μg of Fc blocking mAbs (i.e., anti-CD16/CD32 antibodies, Invitrogen/eBioscience Thermo Fisher) in 100 μL of 1×PBS with 2% FCS for 15 min at 4 °C ...
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bioRxiv - Biophysics 2022Quote: ... Blocking was performed for 2 hours at room temperature with 1X PBS supplemented with 5% Fetal Bovine Serum (Invitrogen) and 0.25% Tween-20 (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... Blocking buffer was replaced after one hour with 7 µg/mL mouse anti-occludin antibody (cat# 33-1500, Invitrogen) in PBS with 5% goat serum ...
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bioRxiv - Neuroscience 2020Quote: ... brains were transferred through 4 wells containing a blocking solution (5% Goat Serum in PBT; Life Technologies #16210-064), and sitting in the last well for 30 minutes ...
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bioRxiv - Neuroscience 2019Quote: ... the tissue was then placed in blocking solution containing secondary antibodies for 24 hr (streptavidin AlexaFluor conjugate from Invitrogen, 1:250 goat anti-mouse AlexaFluor conjugate from Invitrogen) ...
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bioRxiv - Neuroscience 2021Quote: ... After blocking with 3% bovine serum albumin (BSA) in Tris-buffered saline with 0.1% Tween 20 (TBST; Thermo Fisher) for 1 hour ...
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bioRxiv - Developmental Biology 2021Quote: ... followed by incubation with secondary antibodies and conjugated antibodies in blocking buffer (SOX2-GFP, 1:500, eBioscience, cat.no.53-9811-82; Donkey anti-mouse, AF647, ThermoFisher, cat ...
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bioRxiv - Molecular Biology 2022Quote: ... nuclei were washed with blocking buffer and then incubated with anti-rabbit antibody conjugated to Alexa Fluorophore 568 (ThermoFisher) diluted 1:500 for two hours at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... Plates were washed with TBS twice before incubating with serially diluted mAbs in casein blocking buffer (Thermo Fisher Scientific) for 90 min ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The liganded beads were blocked with excess biotin and washed with SEA BLOCK Blocking Buffer (Thermo Fisher, Rockford, IL), 1 % BSA ...
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bioRxiv - Immunology 2021Quote: ... prior to washing in PBS/Tween (0.05% v/v) and blocking with Blocker Casein in PBS (Thermo Fisher Scientific) for 1 h at room temperature (RT) ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were then incubated with Alexa Fluor 647-conjugated donkey anti-rabbit antibody in blocking solution (1:500, Invitrogen) for 1 h in darkness and washed with PBS as described above.
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bioRxiv - Developmental Biology 2021Quote: ... and the sperm was permeabilized in 0.25% Tween in 1x PBS for 30 minutes before blocking with 10% normal goat serum (Invitrogen) and 40 μg/ml BSA in PBST for at least 1 hour ...
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bioRxiv - Cell Biology 2020Quote: ... permeabilized with 0.5% Triton in PBS for 10 min and then blocked in Superblock T20 blocking buffer (Thermo Scientific). Primary and secondary antibodies and ...
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bioRxiv - Immunology 2020Quote: ... prior to washing in PBS/Tween (0.05% v/v) and blocking with Blocker Casein in PBS (Thermo Fisher Scientific) for 1 hour at room temperature (RT) ...
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bioRxiv - Microbiology 2021Quote: ... the coverslips were then stained with an Alexafluor488 goat anti-rabbit antibody (Invitrogen, diluted 1:1000 in blocking buffer).
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bioRxiv - Immunology 2020Quote: ... prior to washing in PBS/Tween (0.05% v/v) and blocking with Blocker Casein in PBS (Thermo Fisher Scientific) for 1 h at room temperature (RT) ...
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bioRxiv - Neuroscience 2021Quote: ... which were then blocked during 1h at RT with Superblock™ in PBS blocking buffer (Thermo fisher Scientific #37515) or PBS containing 0.1% Tween 20 and 3% BSA ...
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bioRxiv - Neuroscience 2022Quote: ... interference from endogenous biotin and/or streptavidin-binding activity was moreover reduced by Avidin/Biotin Blocking kit (Thermo Fisher). Tissue sections were incubated overnight in a humidified chamber with following primary antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were blocked in blocking solution consisting of 10% normal goat serum (New Zealand origin, #16210064, Gibco, Waltham, MA) with 1% bovine serum albumin (Fisher Bioreagents ...
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bioRxiv - Cancer Biology 2022Quote: ... were diluted 1:5000 in 5 % BSA in TBST or in blocking buffer (Cat. no. 37538, Thermo Fisher Scientific) in PBS (1:1 ...