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Citations for Thermo Fisher :
5001 - 5050 of 10000+ citations for RFamide Related Peptide 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Slices were washed again in PBS and incubated for 2 h at room temperature in secondary antibody in carrier solution (donkey anti-goat 647, 1:1000, ThermoFisher; donkey anti-rabbit 405, 1:1000, Abcam; donkey anti-rabbit 555, 1:1000, ThermoFisher). After four final washes ...
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bioRxiv - Cancer Biology 2021Quote: ... blocked with 3% BSA in PBS with 0.1% Tween20 for 1 hour and immunostained with rabbit anti-nestin (1:200, PA-1-86334; Invitrogen, USA), rabbit SOX2 (1:200 ...
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bioRxiv - Physiology 2021Quote: ... We performed the assay using a modified 1-plex protocol for Fast Blue Detection using Type 1 Mouse AGER probe and Type 1 Mouse GAPDH probe (Affymetrix). The sections were counterstained with Nuclear Fast Red ...
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bioRxiv - Developmental Biology 2019Quote: The following primary antibodies were used: anti-ZO-1 (Zymed, #40-2200, 1:100 or Invitrogen, #33-9100, 1:100); anti-N-cadherin (BD Biosciences ...
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bioRxiv - Genomics 2021Quote: ... these droplets were transferred at a density of 6 droplets per 1 well of a 6-well plate into STEP3 medium containing a 1:1 mixture of DMEM/F12 (Invitrogen) and Neurobasal with 0.5X N2 supplement (Invitrogen) ...
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bioRxiv - Microbiology 2021Quote: ... Ten-fold serial dilutions (1×10−1 to 1×10−6) of virus stocks were prepared in MEM (supplemented with 25 mM HEPES (Gibco), 2mM L-Glutamine (Gibco) ...
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bioRxiv - Cell Biology 2020Quote: ... at 1:1000, goat α-rat (AF488, RRID:AB_2534074) at 1:2000, goat α-mouse (AF594, RRID:AB_141372) from Invitrogen (1:2000). Images were quantified using FIJI software (National Institutes of Health ...
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bioRxiv - Cell Biology 2020Quote: ... media was changed gradually into a gradient of (1/4) Neurobasal media (NB: based medium contained 1% Lglutamine, 1% N2 and 2% B27 (all from Invitrogen)) plus reduced gradient of (3/4 ...
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bioRxiv - Microbiology 2020Quote: ... PBMCs were counted and cryopreserved at a concentration of 1×108 cells/ml in 1:1 RPMI and PBMC freezing medium of 85% fetal bovine serum (Gibco) and 15% dimethyl sulfoxide (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2021Quote: ... Primary antibodies were incubated overnight at 40C in 5% NDS/PBS (Nkx2-1 [abcam Cat. Ab227652] 1:500, St18, 1:1000, Ki67 [Invitrogen Cat ...
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bioRxiv - Microbiology 2022Quote: ... mice were given a mixture of antibiotics (ampicillin 1 mg/mL, vancomycin 0.5 mg/mL, neomycin 1 mg/mL, metronidazole 1 mg/mL; Fisher Scientific) in drinking water for 3 consecutive days (Figure 2G).
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bioRxiv - Biochemistry 2022Quote: ... 1:100 dilution of mouse anti- alpha 1 Spectrin (ab11751) and 1:500 dilution of rabbit anti-KCNN4 (76647, Invitrogen). For co- immunostaining experiments with rabbit primary antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 10% Fetal Bovine Serum (EuroBio) and 1% Pen/Strep + 1% Sodium Pyruvate + 1% Non-Essential Amino Acids solution (Gibco) at 37°C in 5% CO2 humidified incubators ...
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bioRxiv - Cell Biology 2022Quote: ... anti-HA(C29F4, Cell Signalling Technology,WB,1:1000, IF,1:500),anti-His (MA1-21315, Invitrogen; WB,1:10,000), anti-EEA1 antibody was a kind gift from Prof ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed with 1 x PBS with 1% FBS and incubated with 1:2000 Alexa Fluor 488-streptavidin (S32354, Invitrogen) in 1 x PBS with 1% FBS for 30 min at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... The 40 mg/ml 4-HT stock solution was diluted 1:1 in Kolliphor EL (MilliporeSigma) 59.Before injection the solution was further diluted 1:3 in PBS (Gibco) and warmed to 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... cultures were incubated in rabbit anti-RIM 1/2 antibody (1:200) followed by donkey anti-rabbit AF647 antibody (1:500; Invitrogen).
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bioRxiv - Neuroscience 2022Quote: ... On day 1, medium was switched to N2-medium (DMEM/F12 without L-glutamine, 1%, Glutamax, 1% N2 (all from Gibco), 0.3% glucose ...
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bioRxiv - Neuroscience 2024Quote: ... and anti-Iba-1 (1:1000, 019-19741, Wako) were visualized using goat-anti-rabbit Alexa Fluor 555 (1:500, A21428, Invitrogen). Wisteria floribunda lectin (1:1000 ...
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bioRxiv - Pathology 2024Quote: ... Antibodies to TOM70 (14528-1-AP, 1:200, Proteintech; Alexa Fluor 488-conjugated secondary antibody: A-11034, 1:500, Invitrogen), and troponin T (1:100 ...
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bioRxiv - Pathology 2024Quote: ... were stained in LV cryosections of 10 µm using a rabbit antibody to TOM70 (14528-1-AP, 1:200, Proteintech; Alexa Fluor 488-conjugated secondary antibody: A-11034, 1:500, Invitrogen) and a mouse Alexa Fluor 647-conjugated antibody to TnT (#565744 ...
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bioRxiv - Bioengineering 2024Quote: ... Tissue constructs were cultured for three weeks in chondrogenic medium (DMEM supplemented with 1% penicillin-streptomycin-glutamine, 1% HEPES (1M), 1% sodium pyruvate (100 mM), 1% ITS (100x) (Insulin, Transferrin, Selenium) (all from Gibco), 0,47 mg/ml linoleic acid (Sigma) ...
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mTORC1-dependent SOCE activity regulates synaptic gene expression and muscle response to denervationbioRxiv - Physiology 2024Quote: ... 1/250 for IF, 1/1000 for WB), SARAF (PA5-24237, 1/250 for IF, 1/1000 for WB) from Invitrogen; Naked2 (#2073 ...
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Regulation of Diseases-Associated Microglia in the Optic Nerve by Lipoxin B4 and Ocular HypertensionbioRxiv - Molecular Biology 2024Quote: ... Sections were then incubated in primary antibodies (anti-CD74, 1:100, BioLegend; anti-Iba1, 1:100; anti-p-PI3K, 1:100, Invitrogen) dissolved in blocking buffer overnight at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... resuspended in 700 μL complete RPMI media (cRPMI; 10% FBS [Gibco], 1% penicillin/streptomycin [Gibco], 1% sodium pyruvate [Gibco], 1% non-essential amino acids [Gibco] ...
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bioRxiv - Cell Biology 2024Quote: ... plates were prepared with 2 % agar in YPD (1 % yeast extract, 1 % peptone, and 1 % glucose) supplemented with 100 μg/mL zeocin (Invitrogen), 300 μg/mL hygromycin B (Invitrogen ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were transfected at a concentration of 106 cells ml-1 with plasmid DNA (1 mg l−1) and 25 kDa linear polyethyleneimine (2 mg l−1) in OptiPRO SFM (Gibco). After 6 d of culture ...
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bioRxiv - Molecular Biology 2023Quote: ... 150 mM NaCl, 1% NP-40, 10% glycerol) and protease inhibitors (1: 1,000 DTT, 1: 100 Halt protease inhibitors (ThermoFisher Scientific), 1 mM PMSF ...
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bioRxiv - Cell Biology 2023Quote: ... Trypsin activity was stopped by diluting 1+1 with a 1 mg/mL solution of soy bean trypsin inhibitor (#10684033 Gibco) in PBS ...
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bioRxiv - Developmental Biology 2023Quote: ... N2B27 (DMEM/F12 [Gibco 11320-074], and Neurobasal in 1:1 ratio [Gibco 21103-049], with 1:200 N2 Supplement [Gibco 17502-048] ...
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bioRxiv - Microbiology 2023Quote: ... membranes were incubated overnight at 4 °C with primary antibodies diluted in 1% NGS in 1% BSA/PBS at 1:50 for rabbit anti-Cytokeratin-20 (CK20) polyclonal antibody (Invitrogen), or 1:30 for mouse anti-uroplakin-III (UPIII ...
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bioRxiv - Bioengineering 2023Quote: ... Cryosections were blocked with 1% bovine serum albumin in PBS for 1 h at room temperature and incubated with primary antibodies (anti-perilipin 1, PA5-72921, 1:200, ThermoFisher, Waltham ...
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bioRxiv - Cell Biology 2023Quote: ... HCT116 cells were transfected with 2μg of total DNA of px330 cas9 and homology arms plasmids (mAID-mClover-HygR and mAID-mClover-NeoR for AID cell line and APEX2-mClover-HygR and APEX2-mClover-NeoR for APEX cell line) in 1:1:1 ratio and selected with 100 μg/mL hygromycin B (Invitrogen) and 700μg/ml of Geneticin (Gibco) ...
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bioRxiv - Cell Biology 2023Quote: ... were grown in high-glucose DMEM with supplements (1% penicillin/streptomycin, 15% FBS, 1% NEAA, 1 mM sodium pyruvate [Gibco] ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were incubated with anti-CD16/32 (FC receptor block) at 1:100 and LIVE/DEAD Fixable (1:400-1:800; cat no., 15519340; Invitrogen) in 20 μl PBS for 10 min ...
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bioRxiv - Cell Biology 2023Quote: ... The following primary antibodies were used for immunofluorescence: anti-ACA (1:300; 15-235; Antibodies Inc.) and anti-CENP-A (1:100, MA 1-20832, Thermofisher). The secondary antibodies used were Alexa Fluor® 488 AffiniPure donkey anti-human IgG ...
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bioRxiv - Neuroscience 2023Quote: ... 47,5% DMEM/F12, 2% B27 w/o Vitamin A, 1% N2 supplement, 1% GlutaMAX, 1% Penicillin/Streptomycin; all from Gibco) supplemented with 10 µM SB-431542 (Torics Bioscience) ...
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bioRxiv - Neuroscience 2023Quote: ... six-well plates containing prewarmed neural differentiation medium (NDM; Neurobasal A, 2% B27, 1% N2, 1% Glut Amax, 1% P/S; all from Gibco) supplemented with 20 ng/mL of EGF and FGF2 (both PeproTech ...
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bioRxiv - Neuroscience 2023Quote: ... 47,5% DMEM/F12, 2% B27 w/o Vitamin A, 1% N2 supplement, 1% GlutaMAX, 1% Penicillin/Streptomycin; all from Gibco) supplemented with 10 µM SB-431542 (Tocris Bioscience) ...
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bioRxiv - Neuroscience 2023Quote: ... the spheroids were transferred to non-adhesive 100 mm dishes containing Neural differentiation medium (NDM; Neurobasal A, 2% B27, 1% N2, 1% GlutaMAX, 1% P/S all from Gibco) supplemented with 20 ng/mL of EGF and FGF2 (both PeproTech ...
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bioRxiv - Cell Biology 2023Quote: ... Selection of positive transformants was performed on YPD plates (1% Yeast extract, 1% Peptone, and 1% Dextrose) containing 100 μg/ml zeocin (Invitrogen) and/or nourseothricin (Werner Bioagents) ...
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bioRxiv - Cell Biology 2023Quote: ... Shinkai) were grown in high-glucose DMEM with supplements (1% penicillin/streptomycin, 10% FBS, 1% NEAA, 1% L-glutamine [Gibco] ...
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bioRxiv - Developmental Biology 2023Quote: ... Samples were labeled with fluorescently-conjugated antibodies for 30 min at 4C-Sca1-APCCy7 (1:1500, eBioscience), α6-PE (1:1000, eBioscience) and α6-FITC (1:200, Invitrogen), β1-Alexa647 (1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... and plated on Matrigel-coated dishes in Induction Media (DMEM/F12 supplemented with 1% N2-supplement [GIBCO], 1% NEAA [GIBCO], and 1% GlutaMAX [GIBCO] ...
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bioRxiv - Developmental Biology 2024Quote: ... fluorescent secondaries were added overnight at 4°C (goat-anti rabbit AlexaFluor 647 1:500, goat anti-mouse AlexaFluor 568 1:500, goat anti-guinea pig AlexaFluor 594 1:500; all ThermoFisher). Ovarioles were hand-dissected from whole fixed ovaries prior to imaging.
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bioRxiv - Physiology 2024Quote: ... and incubated overnight at 4°C in primary antibodies (1:500 anti-NALCN, Alomone labs; 1:4000 anti-SLO2.1, Absolute Antibody; 1:50 alpha-Smooth muscle actin, Invitrogen). Slides were incubated in biotinylated secondary antibodies in 10% GS for 30 min at room temperature ...
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bioRxiv - Immunology 2024Quote: ... Cells were plated in complete (c)RPMI (RPMI supplemented with 10% FBS, 1% sodium pyruvate, 1% HEPES and 1% L-glutamine, all GIBCO) supplemented with Penicillin-Streptomycin (100 U mL-1 ...
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bioRxiv - Neuroscience 2024Quote: ... or anti-mouse IgG (1:300; A21202) or anti-rabbit IgG (1:500; A21206) or anti-rat IgG (1:500; A21208, both Invitrogen), Alexa Fluor 594-labeled donkey anti-goat IgG (1:300 ...
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bioRxiv - Cancer Biology 2021Quote: ... L-WRN CM was diluted 1:1 in Advanced DMEM/F12 (Gibco) and supplemented with additional RSPO-1 conditioned media (10% v/v) ...
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bioRxiv - Cell Biology 2020Quote: ... In more details: rabbit anti-ZO-1 (1/500, 61-7300, Invitrogen), mouse anti-occludin (1/500 ...