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Citations for Thermo Fisher :
5001 - 5050 of 5129 citations for JIP3 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2019Quote: ... Cells or tissue were rinsed in TX/PBS and incubated in blocking solution containing secondary antibody (Alexa conjugated, Life Technologies, 1:1000, 2 hours, RT). After staining ...
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bioRxiv - Physiology 2021Quote: ... After primary incubations the RBCs were washed three times in PBS and incubated for three hours on a rotator at room temperature in blocking buffer containing secondary antibodies: 1:500 goat anti-mouse (AlexaFlour 568; Thermo Fisher Life Technologies A-11031), 1:500 goat anti-rabbit (AlexaFlour 488 ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were subjected to blocking in 0.1M phosphate buffer with 0.3% Triton X-100 (PBTx) containing 10% horse serum (Thermo Fisher Scientific, Cat. No. 26-050-088) and 0.5% bovine serum albumin (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated for 1.5 hours at RT with the corresponding secondary fluorescent antibodies diluted 1:1000 in blocking solution: goat anti-chicken Alexa FluorTM 568 nm (Invitrogen ref. A-11041, RRID AB_2534098), goat anti-rabbit Alexa FluorTM 647 nm (Invitrogen ref ...
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bioRxiv - Immunology 2024Quote: ... The membranes were placed in an opaque gel box and incubated with blocking buffer (PBST + 10% Fish gelatin (PBS 10X, pH 7.2, Thermo Fisher Scientific, Cat-No. 70-013-032; Biotium 10X Fish Gelatin Blocking Agent, Thermo Fisher Scientific, Cat-No. NC0382999) at room temperature for 1 hour ...
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bioRxiv - Cell Biology 2023Quote: ... with a monoclonal antibody against B5 (19C2, rat, 1:1000; (Schmelz et al. 1994)) followed by an Alexa Fluor 647 anti-rat secondary antibody (Invitrogen; 1:1000 in blocking buffer) prior to permeabilization of the cells with detergent ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cells were then stained with mouse monoclonal anti-CFTR antibody 570 (Cystic Fibrosis Foundation; 1:200 in above blocking buffer) and rabbit polyclonal anti-ZO-1 antibody (ThermoFisher Scientific #40-2200 1:200) for 2 h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were then again transferred to new wells containing the blocking solution plus secondary antibodies (goat anti-mouse Alexa Fluor 594 (Thermo Fisher Scientific Cat. #A-11032) at 1:1000 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... followed by the addition of secondary antibodies in blocking buffer (goat-anti-rabbit-IgG-AlexaFluor488 [ThermoFisher Scientific #A11034] and goat-anti-mouse-IgG-AlexaFluor568 [ThermoFisher Scientific #A11031] both 1:200) and Hoechst 33342 (2 ug/mL from 10 mg/mL stock ...
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bioRxiv - Biochemistry 2024Quote: ... the cells were stained with a monoclonal antibody against B5 (19C2, rat, 1:1000 (Schmelz et al, 1994)) followed by an Alexa Fluor 647 anti-rat secondary antibody (Invitrogen; 1:1000 in blocking buffer). Cells were then permeabilized with 0.1% Triton-X/PBS for 3 min ...
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bioRxiv - Biochemistry 2022Quote: ... peptides were loaded on an Acclaim PepMap 100 μ-precolumn cartridge (5 μm, 100 Å, 300 μm ID x 5 mm, Thermo Fisher Scientific). Then ...
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bioRxiv - Neuroscience 2021Quote: The remainder of the light-labeled samples were mixed with the mixture of heavy labeled spike peptides and applied to High-Select™ Fe-NTA Phosphopeptide Enrichment Kit (Thermo fisher Scientific, U.S.A.) to enrich the phosphorylated peptides ...
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bioRxiv - Plant Biology 2019Quote: ... ~100 pmoles) of the peptide samples were separated online using a Dionex Ulimate 3000 RSLCnano pump and autosampler (Thermo Fisher Scientific, Waltham, MA, USA) and a column packed in-house utilizing ProntoSIL C18AQ ...
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bioRxiv - Microbiology 2019Quote: ... Eluted peptides were analysed by liquid chromatography tandem mass spectrometry (LC-MS/MS) on a Dionex UltiMate 3000 RSLCnano System (Thermo Scientific, Waltham, MA, USA) coupled to an Orbitrap Q Exactive mass spectrometer (Thermo Scientific ...
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bioRxiv - Cancer Biology 2021Quote: Tryptic digestion of proteins from samples were followed by a nanoflow LC/MS-MS analysis of peptides using the quadrupole Orbitrap mass spectrometer (Q Exactive HF-X, Thermo Fisher Scientific, Bremen, Germany) (Kelstrup et al ...
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bioRxiv - Neuroscience 2020Quote: ... LC-MS/MS analyses of the generated peptides were performed on a hybrid dual-pressure linear ion trap/orbitrap mass spectrometer (LTQ Orbitrap Velos Pro, Thermo Scientific, San Jose, CA) equipped with an EASY-nLC Ultra HPLC (Thermo Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... The digested peptides were desalted using Oasis HLB cartridges (Waters, Milford, MA, USA) and then dried by vacuum evaporation (Thermo Fisher Scientific, Bremen, Germany).
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bioRxiv - Microbiology 2020Quote: ... Analytical separation of peptides was done on an Acclaim® PepMap RSLC column (75 µm × 50 cm, C18, 3 µm, 100 Å; Thermo Fisher Scientific) running a water-acetonitrile gradient at a flow rate of 300 nl/min ...
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bioRxiv - Microbiology 2020Quote: ... The eluted peptides were electrosprayed at 2 kV into a dual linear quadrupole ion trap Orbitrap Velos Pro mass spectrometer (MS) (Thermo Fisher, San Jose, CA). The MS was set to switch between a full scan (400 < m/z > 2000 ...
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bioRxiv - Microbiology 2020Quote: The fractionated peptides were separated and analyzed using a Dionex Ultimate 3000 RSLCnano system coupled to Q Exactive tandem mass spectrometry (Thermo Fisher Scientific, MA, USA). Separation was performed on a Dionex EASY-Spray 75 μm × 10 cm column packed with PepMap C18 3 μm ...
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bioRxiv - Microbiology 2020Quote: ... A total of 100 μg protein from each condition was subjected to in-solution trypsin digestion before labelling the resultant tryptic peptides using the TMT-6plex Isobaric Label Reagent Set (Thermo Scientific, Rockford, IL, USA) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... The digested peptides were desalted using Oasis HLB cartridges (Waters, Milford, MA, USA) and then dried by vacuum evaporation (Thermo Fisher Scientific, Bremen, Germany).
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bioRxiv - Evolutionary Biology 2022Quote: A 2-μg aliquot of the peptide mixture was analyzed using a LTQ-Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific, San Jose, CA) coupled to an EASY-nLC 1000 (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2022Quote: ... approximately 1.5 μg of peptide per sample was injected into Ultra-High-Performance Liquid Chromatography (UHPLC) system (Ultimate™ 3000 RSLCnano, (Thermo Fisher Scientific, USA) coupled online with Impact HD™ UHR-QqToF (Bruker Daltonics ...
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bioRxiv - Plant Biology 2022Quote: Coding sequences of mature proteins without signal peptide were amplified with primers listed in Table S1 and cloned into expression vector pETSUMO (Thermo Fisher Scientific, Massachusetts, USA). Correctness of the resulting constructs pETSUMO-ChElp2 and pETSUMO-Vd2LysM were confirmed by DNA sequencing and introduced into E ...
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bioRxiv - Plant Biology 2021Quote: A small fraction of all samples were pooled and fractionated using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Pierce, Thermo Fisher Scientific, San Jose). Briefly ...
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bioRxiv - Neuroscience 2020Quote: ... The digested peptides were desalted using Oasis HLB cartridges (Waters, Milford, MA, USA) and then dried by vacuum evaporation (Thermo Fisher Scientific, Bremen, Germany).
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bioRxiv - Immunology 2020Quote: ... The pool of peptides generated in the degradation assay was assessed by mass spectrometry using a Q-Exactive Orbitrap Mass Spectrometer (Thermo Fisher Scientific, Bremen, Germany) and nano-HPLC (Dionex Ultimate 3000 ...
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bioRxiv - Systems Biology 2022Quote: The resulting peptides were dried to 12 µL and analyzed by nanoLC-MS/MS using a Dionex Ultimate 3000 (Thermo Fisher Scientific, Bremen, Germany) coupled to an Orbitrap Eclipse Tribrid mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... and the QI9 peptide (QYIKWPWYI, residues 1208-1216 of the SARS-CoV-2 spike protein) maintained in RPMI 1640 medium (Thermo Fisher Scientific, Cat# 11875101) containing 10% FBS and 30 U/ml recombinant human IL-2 (Peprotec ...
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bioRxiv - Immunology 2022Quote: ... 2 μg of peptide material of each sample was injected for LC-MS/MS analysis on an Ultimate 3000 RSLC nano-LC (Thermo Fisher Scientific, Bremen, Germany) in-line connected to a Q Exactive HF mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... brain-derived tryptic peptides were separated using a NanoAcquity UHPLC (Waters, Milford, FA) and monitored on a Q-Exactive Plus mass spectrometer (ThermoFisher Scientific, San Jose, CA). Raw data were analyzed using MaxQuant v1.5.2.8 with Thermo Foundation 2.0 for RAW file reading capability17 ...
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bioRxiv - Biochemistry 2020Quote: ... The peptides were collected and desalted on Oasis HLB cartridges (Waters, Milford, MA) and then dried by vacuum evaporation (Thermo Fisher Scientific, Bremen, Germany) and stored at −80°C.
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bioRxiv - Microbiology 2020Quote: ... Peptides were extracted from the gels and analysed by LC-MS/MS on an Orbitrap-Fusion™ mass spectrometer (Thermo Fisher, Hemel Hempstead, UK) equipped with an UltiMate™ 3000 RSLCnano System using an Acclaim PepMap C18 column (2 µm ...
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bioRxiv - Microbiology 2020Quote: ... The extracted peptides from OE and OB groups were labeled with TMT10 reagents according to the manufacturer’s instructions (Thermo Scientific, San Jose, CA, USA), respectively ...
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bioRxiv - Cancer Biology 2019Quote: ... The peptides were desalted by an HLB column (Waters, Milford, MA) and were then drained by a vacuum pump (Thermo Fisher Scientific, Bremen, Germany). The peptides were acidified with 0.1% formic acid and were loaded onto a reversed-phase microcapillary column using a Thermal EASY-n LC1200 chromatography system ...
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bioRxiv - Cell Biology 2020Quote: Nanoflow LC–MS/MS analysis of tryptic peptides was conducted on a quadrupole Orbitrap mass spectrometer (Q Exactive HF-X, Thermo Fisher Scientific, Bremen, Germany)14 coupled to an EASY nLC 1200 ultra-high-pressure system (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Digestion was stopped by the addition of 2% (v/v) formic acid before peptides were cleaned using Pierce C18 spin columns (Thermo Fisher Scientific, cat#89870) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Peptides were then directly labeled as follows: acetonitrile was added to 30% (v/v) concentration and peptides were labeled with TMT 10-plex reagent (Thermo Fisher Scientific, Catalog #90406) for 1 hour ...
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bioRxiv - Physiology 2020Quote: ... Antibodies to the antigen peptide were purified from the final bleed antiserum by affinity-purification using the AminoLink Plus Immobilization Kit (ThermoFisher Scientific, Waltham, MA, USA), with bound antibodies eluted using glycine elution buffer [6.3 ml of 100 mM glycine (VWR Chemicals ...
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bioRxiv - Genetics 2021Quote: ... The separated peptides were loaded onto a C18 column (50 cm × 75 μm) connected to an EASY-nLC™ 1200 system (Thermo Fisher Scientific, USA) and separated with a linear gradient of buffer B (80% acetonitrile and 0.1% formic acid ...
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bioRxiv - Biochemistry 2021Quote: Separation of digested histone peptides was performed by using nanoliter flow liquid chromatography using an EASY-nLC nanoHPLC (Thermo Scientific, San Jose, CA, USA) equipped with an analytical weak cation exchange–hydrophilic interaction liquid chromatographic resin (PolycatA ...
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bioRxiv - Developmental Biology 2021Quote: ... One microgram of peptide was injected into an Ultimate 3000 RSLC chromatography system connected to a Q Exactive HF-X mass spectrometer (Thermo Scientific, Waltham, MA, USA). Samples were transferred to a PepMap 100 C18 trap column (100 µm x 2 cm ...
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bioRxiv - Biochemistry 2020Quote: Peptides and cross-linked peptides were analyzed using an UltiMate3000 RSLCnano (Dionex, Sunnyvale, CA) coupled to an Orbitrap Fusion (Thermo-Fisher Scientific, San Jose, CA) mass spectrometer equipped with a nanospray Flex Ion Source ...
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bioRxiv - Developmental Biology 2022Quote: ... The digested peptides were desalted using Oasis HLB cartridges (Waters, Milford, MA, USA) and then dried by vacuum evaporation (Thermo Fisher Scientific, Bremen, Germany).
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bioRxiv - Molecular Biology 2022Quote: ... and fragmented to produce a tandem mass-spectrum of specific fragment ions for each peptide with LTQ Orbitrap Velos Pro ion-trap mass spectrometer (Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Immunology 2022Quote: ... the RBD was inserted into pcDNA3.4 with a streptavidin binding peptide (SBP) tag at the C-terminus and were produced using the Expi293 Expression System (Thermo Fisher Scientific, Waltham, MA) according to manufacturer’s instruction ...
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bioRxiv - Neuroscience 2024Quote: ... Peptides were loaded onto a C18 pre-column (3 μm, 75 mm i.d. × 2 cm, 100 Å, Thermo Fisher Scientific Cat# 16-494-6) then separated on a reverse-phase nano-spray column (2 μm ...
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bioRxiv - Neuroscience 2024Quote: LC-MS/MS analysis for DDA Peptides from each pooled fraction were first spiked with iRT standard peptides (Biognosys AG, Switzerland) and separated using reverse-phase high-performance liquid chromatography (RP-HPLC) on EASY-nLC system (Thermo Fisher Scientific, Bremen, Germany) with a column (75 μm × 150 mm ...
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bioRxiv - Cancer Biology 2024Quote: Tandem Mass Tag (TMT)-10 labeling of peptides was conducted as follows: The TMT-10 plex Isobaric Label Reagent (Catalog No. 90110, Thermo Fisher Scientific, Waltham, MA) was dissolved in anhydrous acetonitrile (ACN ...