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Citations for Thermo Fisher :
5001 - 5050 of 5189 citations for CD40L Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The cells stably expressing human recombinant LOXL1 were maintained in culture (37°C, 5% CO2) at log-phase growth in advanced DMEM (Thermo Fisher Scientific, 12491015) with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2023Quote: ... SH-SY5Y cells were transfected with either an empty vector or with a vector expressing His-tagged human p35 (gift from Dr. Harish Pant) using Lipofectamine 3000 (Life Technologies, Carlsbad, CA). Two days after the transfection ...
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bioRxiv - Microbiology 2023Quote: ... The level of IL-8 in cell culture supernatants was determined using a Human IL-8 Uncoated ELISA kit (Invitrogen, ThermoFisher Scientific Inc.).
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bioRxiv - Microbiology 2023Quote: ... Binding of VHH-Fcs or antibodies was detected using an AF633 donkey goat anti-human IgG antibody (Iinvitrogen, A21091, 1/1000) and dead cells were stained using Live/Dead stain (Invitrogen, 15560607, 1/1000). Following 3 washes with PBS containing 0.5% BSA ...
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bioRxiv - Immunology 2023Quote: ... Quantitative gene expression for mouse and human genes was performed using TaqMan probes and TaqMan Fast Advanced Master Mix (Applied Biosystems/Thermo Fisher, 4444554) with a QuantStudio 6 Pro (Applied Biosystems) ...
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bioRxiv - Immunology 2023Quote: ... Freshly isolated CD8+ T cells were differentiated in vitro to CTLs by incubation for 48 h with anti-CD3/CD28 coated magnetic beads (Dynabeads Human T-activator CD3/CD28, Gibco, Waltham, Massachusetts, USA) for cell expansion and activation and 50 U/mL of IL-2 (human Interleukin-2 ...
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bioRxiv - Immunology 2023Quote: The IgG antibodies were extracted from a pool of 100 healthy human serum samples using the Melon™ Gel IgG Spin Purification Kit (Thermo Fisher Scientific), with a tandem purification (one after another ...
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bioRxiv - Immunology 2023Quote: ... cells at the end of 1-day culture were pulsed for 20 minutes with 40 ng/ml of recombinant human IFN-γ (Gibco/Thermo Fisher), 100 IU/ml of recombinant human IL-2 (Roche Diagnostics ...
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bioRxiv - Biophysics 2023Quote: Full-length human MRS2 (Uniprot Q9HD23) conjugated with a FLAG tag at the C-terminus was expressed in Expi293F cells (Thermo Fisher Scientific, A14527). Cells at a density of 2.0-3.0 × 106 cells/ml were transfected with the MRS2-encoding pCMV plasmid using polyethylenimine (PEI ...
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bioRxiv - Genomics 2023Quote: ... Cells were rinsed once in staining buffer and incubated with 2 µg/ml anti-human CD326 conjugated with AlexaFluor 700 (ThermoFisher 56-9326-42) at 4C in the dark for 1h ...
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bioRxiv - Microbiology 2023Quote: Isolates from wastewater and isolates provided from collaborators (8 animal/food, 6 environment, 1 human skin, 137 UTI) were grown in tryptic soy broth (TSB; Thermo Scientific; cat. CM0129B) for 24 – 48 hours at 37°C to an OD600 of ∼1 ...
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bioRxiv - Biochemistry 2023Quote: Human embryonic kidney cells (HEK293T, ATCC, Cat#CRL-3216) were cultured in Dulbecco’s modified eagle medium (DMEM, Thermo Fisher Scientific, Invitrogen, Cat#11965092) supplemented with 10% (v/v ...
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bioRxiv - Biochemistry 2023Quote: ApoE was immunoprecipitated from matched participant samples of 20 μL CSF and 2 μL plasma via a monoclonal antibody to human apoE (HJ15.3) (provided by the Holtzman Lab) conjugated to M-270 Epoxy Dynabeads (Life Technologies/Invitrogen, Carlsbad, CA). The CSF samples were prepared by combining 20 μL of neat CSF with 100 μL of the apoE3*/apoE4* astrocyte media internal standard ...
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bioRxiv - Immunology 2023Quote: ... the mice received an intravitreal injection of either human monocytes or hMdɸs that were labeled with the Vybrant DiO tracer (Invitrogen-Molecular Probes, Carlsbad, CA), and then returned to the standard light/dark cycle ...
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bioRxiv - Cell Biology 2023Quote: SEES2 ESCs24 were cultured on irradiated MEFs (irrMEFs) with medium for human ESCs: Knockout-Dulbecco’s modified Eagle’s medium (KO-DMEM: 10829-018, Life Technologies; Thermo Fisher Scientific, MA, USA) supplemented with 20% Knockout-Serum Replacement (KO-SR ...
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bioRxiv - Microbiology 2022Quote: ... cells were washed once by Hanks’ Balanced Salt Solution (HBSS) and then incubated with 2 μg/mL of Alexa Fluor 488-conjugated goat anti-human IgG (Thermo Fisher Scientific; A11013) or DyLight 594-conjugated goat anti-human IgG (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: Primary human CD4+ T cells expressing CBh-ZIM3-dCas9-Bsd (transduced and selected as described above) were activated with Dynabeads Human T-Activator CD3/CD28 (Thermo Fisher Scientific, 11131D) at 1:1 cell:bead ratio and ...
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bioRxiv - Biophysics 2023Quote: The nucleotide sequence encoding the human ADORA2A (1-316 aa) (UniProt ID C9JQD8) was codon optimized for Leishmania tarentolae expression by the GeneOptimizer software (Thermo Fisher Scientific, USA) and synthesized de novo (Eurofins ...
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bioRxiv - Physiology 2023Quote: ... HEK293 cells stably expressing the human hERG channel (Bioprojet, France) were cultured in the same medium with 400 µg/mL G418 (Thermo Fisher Scientific, France), at 5% CO2 ...
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bioRxiv - Immunology 2023Quote: ... or VHHASC with a C-terminal HA tag or C-terminal EGFP fusion under the control of the human elongation factor-1 α promoter (pEF1α) were generated using lentiviral vectors constructed by Gateway cloning (Thermo Fisher Scientific) using vectors modified from pRLL (a kind gift of Susan Lindquist ...
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bioRxiv - Cell Biology 2023Quote: ... The human nasal septum epithelial cell line RPMI 2650 (ATCC CCL-30) cells was cultured in Advanced MEM culture medium (Gibco™ 12492-013) supplemented with 2.5% FCS and 4 mM L-glutamine ...
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bioRxiv - Cell Biology 2023Quote: ... Descemet’s membrane with adherent endothelial cells were stripped from the corneoscleral buttons and incubated overnight at 37°C in minimal medium (Human Endothelial SFM, Gibco, cat# 11111-044); 2% charcoal stripped fetal bovine serum (FBS) ...
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bioRxiv - Physiology 2023Quote: Confluent HUVECs were stimulated by 50 ng/ml human VEGF-A for indicated lengths of time and lysed in M-PER buffer (Thermo Fisher Scientific, USA) supplemented with protease and phosphatase inhibitors (Mini Complete/PhosphoStop ...
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bioRxiv - Zoology 2023Quote: ... Supernatants were collected 24 hours post-treatment and then IL-8 secretion was quantified using commercial human IL-8 ELISA kits (Invitrogen, cat. #88-8086) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... were determined by matching the UniProt human protein database (release 2023_01) with the acquired fragmentation pattern using Sequest (Thermo Fisher Scientific, Waltham, MA) (Eng et al. ...
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bioRxiv - Neuroscience 2024Quote: ... The GFP plasmid and GPRC6A-Myc plasmid were transfected into the HEK293T that stably expresses a tetracycline-inducible wild-type human tau (4R0N) (iHEK-Tau) using Lipofectamine™ 2000 Transfection Reagent (Invitrogen™, #11668019). Doxycycline was added to the cells to induce tau production on the first day of transfection ...
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bioRxiv - Microbiology 2023Quote: Human pluripotent stem cells (hPSCs) lines, IMR90 (IMR90-4, WiCell), H1 (WAe001-A, WiCell) and Gibco (Gibco® Episomal hiPSC Line, Gibco®) were routinely cultured on treated 6-well tissue culture plates (CoStar® ...
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bioRxiv - Molecular Biology 2023Quote: ... RT-PCR samples were prepared using Taqman 1-Step qRT-PCR master mix and Taqman gene expression assays for human TBP (Invitrogen, cat no. Hs00427620_m1). Custom primers and probes were ordered from Genscript to quantify the different splice variants (see Table S1 for sequences and Figure 2 for alignment on the minigene sequence) ...
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bioRxiv - Molecular Biology 2023Quote: A total of 3×105 CD45Δ T cells (from 2 healthy donors) and T cells (from 2 healthy donors) (effector cells) were mixed with Dynabeads Human T Activator CD3/CD28 (Thermo Fisher Scientific, USA) on the 8th-16th days after knockout of CD45 (E:beads ratio was 1:1 ...
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bioRxiv - Cell Biology 2023Quote: ... Ovine (Fig. 5) and human Pcells (supplement Fig.5S) were treated with monoclonal mouse SERCA2 antibody (clone 2A7-A1; Thermo Fisher; see supplement) and secondary fluorescent antibody Alexa Fluor® 568 Goat Anti-Mouse IgG (H+L ...
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bioRxiv - Microbiology 2024Quote: ... The human mAb 860-55D against intact capsids was purchased from Mikrogen (Neuried, Germany) and detected using the pAb Alexa Fluor® 488 conjugated goat anti-human (A11013, Thermo Fisher Scientific). The mouse mAb 3113-81C against viral capsid proteins was obtained from US Biological (Boston ...
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bioRxiv - Cell Biology 2024Quote: ... The hTERT RPE-1 human epithelial cell line (ATCC-CRL4000) was obtained from ATCC and grown in DMEM/F12 (ThermoFisher Scientific, Carlsbad, CA) with 10% fetal bovine serum (FBS) ...
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bioRxiv - Developmental Biology 2024Quote: ... 5% CO2, and 95% humidity. GFP labeled Human Brain Vascular Pericytes (HBVP) (ScienceCell™, Cat. #: 1200) were grown in DMEM (Gibco, Cat. # 11995065) supplemented with 10% FBS and 1X Antibiotic-Antimycotic under the cell culture conditions indicated above ...
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bioRxiv - Cell Biology 2024Quote: ... Human embryonic kidney (HEK) 293T cells were from ATCC (cat. #CRL-3216) and were cultured in high-glucose DMEM (Gibco, cat. #41966-052) supplemented with 10% FBS and 1% Penicillin-Streptomycin.
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bioRxiv - Cancer Biology 2024Quote: ... Samples were then handled by the University of Colorado Anschutz Genomics and Microarray Core for microarray analysis using a Human Clariom D chip (902922; Applied Biosystems via ThermoFisher). This array has over 540,000 transcripts on it including mRNAs ...
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bioRxiv - Microbiology 2024Quote: ... cells were washed once with Hanks’ Balanced Salt Solution (HBSS) and incubated with 1 μg/mL of Alexa Fluor 488-conjugated goat anti-human IgG (Thermo Fisher Scientific; A11013) diluted in HBSS/1% BSA for 1 hour at 37℃ ...
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bioRxiv - Neuroscience 2024Quote: ... Peptide arrays were washed 3 times for 5 min at RT with TBST and incubated with the antibody Rabbit anti-Human IgG DyLight™ 800 (Cat# SA5-10116, Thermofisher, 1/1000) diluted in TBST ...
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bioRxiv - Biochemistry 2024Quote: ... cells were incubated for 1 hour sat room temperature with the following secondary antibodies: anti-goat anti-human Alexa Fluor 647 (Invitrogen, Carlsbad, California, USA). All washing steps were performed with PHEM-T buffer ...
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bioRxiv - Immunology 2024Quote: ... Goat anti-human IgG conjugated with horseradish peroxidase (HRP) was used as the secondary antibody at a dilution of 1:2000 (Life technologies, Ref H10307) in PBS-T-milk for 1 hour at RT ...
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bioRxiv - Molecular Biology 2024Quote: Human osteosarcoma cell line U2OS (ATCC-HTB-96) and human colorectal carcinoma cell line HCT116 (ATCC-CCL-247) were cultured in DMEM (Gibco, 11-965-092) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2021Quote: ... in CD-1 mouse, Sprague−Dawley rat, and human plasma (Bioreclamation, Inc., Hicksville, NY) by equilibrium dialysis using the RED Device (Thermo Fisher Scientific, Rockford, IL). Compounds were added to pooled plasma (n ≥ 3 ...
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bioRxiv - Developmental Biology 2021Quote: ... the causal relation that a target gene is up- or downregulated depending on the transcription complex being active or inactive and (ii) the causal relation that a signal from a gene expression microarray (e.g. Affymetrix Human Genome U133 Plus 2.0) probeset is high or low depending on the target gene being up or down ...
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bioRxiv - Cell Biology 2020Quote: ... α-SC35 (described previously98, Mouse 1:1000), α-NIN (Institut curie, Human 1:200), anti-Arl13b (proteintech, Rabbit, 1:4500) α-Rabbit Alexa594 (ThermoFisher, 1:400), α-Mouse IgG1 Alexa488 (ThermoFisher ...
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bioRxiv - Cell Biology 2020Quote: ... In a second set of experiments Human HK-2 cells were cultured in DMEM and Keratinocyte-SFM (1X) medium (Life Technologies Green Island NY). When the cells reached 70% confluence ...
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bioRxiv - Cell Biology 2020Quote: Total RNA extracted from EndoC-βH1 and collagenase-isolated human pancreatic islets samples was quantified using Qubit 3000 Fluorometer (ThermoFisher Scientific, Waltham, MA, USA), while those extracted from LCM-islets were quantified using 2100 Bioanalyzer-RNA 6000 Pico Kit (cat ...
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bioRxiv - Developmental Biology 2021Quote: ... For cells freshly purifed from human lung tissues were directly lysed using 100 μl lysis buffer from PicoPure™ RNA Isolation Kit (Thermo Fisher Scientific, KIT0204). First Strand cDNA synthesis was performed using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Molecular Biology 2022Quote: ... according to manufacturer’s protocol Following ChIP-valuated antibodies were used for precipitation: mouse-anti-human H3K9/14/18/23/27ac (Thermo Fisher Scientific, Carlsbad, CA, USA) and mouse-anti-IgG (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... Blots were probed with primary antibodies: human anti-SARS-CoV-2 Spike (S-ECD/RBD) (cat# bcb03, Thermo Fisher, 1:1000, non-reducing conditions), anti-Salmonella typhimurium LPS (cat# ab8274 ...
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bioRxiv - Molecular Biology 2020Quote: ... falciparum asexual blood stages were cultured in human erythrocytes (National Blood Transfusion Service, London, United Kingdom) and complete medium (CM) consisting of RPMI-1640 medium (Life Technologies, California, United States) supplemented with 0.5% AlbuMAX type II (Gibco) ...
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bioRxiv - Genomics 2020Quote: ... Human DNA was removed using DNase I and RNA concentration was measured using Qubit RNA HS Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA). DNA-depleted and purified RNA was used to construct double-stranded DNA library using MGIEasy RNA Library preparation reagent set (MGI ...