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Citations for Thermo Fisher :
5001 - 5050 of 10000+ citations for 6 Bromo 3 N ethylamino 1 2 4 triazolo 4 3 a pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Peptide mixtures were injected in 0.1% TFA on a C18 Acclaim PepMap100 trap-column (75 μm ID x 2 cm, 3 μm, 100Å, Thermo Fisher Scientific) for 3 min at 6 μL/min with 2% ACN ...
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bioRxiv - Neuroscience 2022Quote: OE sections from mice injected with retrograde tracer were stained with Alexa Fluor 555- conjugated streptavidin to detect biotin-labeled dextran (1:500 in phosphate-buffered saline containing 3 % normal donkey serum and 0.3 % Triton-X100 for 2 h at room temperature, S32355, Thermo Fisher Scientific), or GAP43 (see above ...
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bioRxiv - Neuroscience 2024Quote: ... Control 1 (C1) and 2 (C2) were derived by StemBANCC from an unaffected volunteer and control 3 (C3) was purchased from Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... Fractionated samples and bead-enriched samples were individually loaded onto a C18 trap column (3 μm, 75μm × 2 cm, Thermo Fisher Scientific) connected in-line to a C18 analytical column (2 μm ...
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bioRxiv - Immunology 2024Quote: ... We electroporated 3 μg of ODNs or gDNA into 2 × 106 HEK293 and 293A cells by Neon Transfection System (Thermo Fisher) based on the manufacturer’s introductions.
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bioRxiv - Neuroscience 2024Quote: ... Each sample was loaded onto an Acclaim PepMap trap column (75 µm × 2 cm, 3 µm, C18, 100 Å, Thermo Scientific) and then separated on a nanoEase BEH C18 column (150 µm × 100 mm ...
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bioRxiv - Neuroscience 2024Quote: ... either for 2-3 cm or ran completely and stained with colloidal coomassie dye G-250 (Gel Code Blue Stain Reagent, Thermo Scientific). The lane was cut into bands ...
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bioRxiv - Molecular Biology 2024Quote: Protein concentrations from fractions 2 and 3 of fed human milk and digesta were determined using a Micro BCATM kit (Pierce, Thermo Fisher), following manufacturer instructions.
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bioRxiv - Biochemistry 2023Quote: ... cells were passaged every 2–3 days by washing twice with phosphate-buffered saline (PBS) and dissociation with 0.25% Trypsin (Life Technologies, 25200056).
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bioRxiv - Cancer Biology 2024Quote: ... Recipient cells were incubated with either PKH67 dye-labelled or fluorescent-labelled sEVs for 2-3 days in media supplemented with 5% exosome-depleted FBS (Gibco, USA). Cells were imaged with time-lapse microscopy or washed with PBS ...
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bioRxiv - Cancer Biology 2024Quote: For acute versus chronic proliferation measurements total cell number for acute and chronic was counted at day 0 and every 2-3 days until day 10 using Countess automated cell counter (Thermo Fisher). For Incucyte® proliferation assay plastic flat bottom 96 well plates were coated with 5 μg/mL human fibronectin in PBS for 1h in 37°C ...
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bioRxiv - Microbiology 2023Quote: ... Samples were injected and concentrated on a trap column (PepMap100 C18, 3 μm, 100 Å, 75 μm i.d.×2 cm, Thermo Scientific) equilibrated with 0.05% trifluoroacetic acid in water ...
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bioRxiv - Cell Biology 2022Quote: ... Single-axis tilts were collected from −60° to +60° at 2° increments at 7 □µm defocus on a Falcon 3 camera (Thermo Fisher) operated in linear mode at 22,000X magnification ...
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bioRxiv - Molecular Biology 2023Quote: ... Peptides were injected onto an Acclaim PepMap100 C18 trap column (75 μm i.d., 2 cm long, 3 μm, 100 Å, Thermo Scientific) and further separated on an Acclaim PepMap100 C18 analytical column (75 μm i.d. ...
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bioRxiv - Molecular Biology 2022Quote: ... C18, 3 μm, 100A), then separated using analytical column Acclaim PepMap RSLC (75um x25cm, nanoViper, C18, 2 μm, 100A) (Thermo Fisher). The flow rate was 300 nL/min ...
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bioRxiv - Plant Biology 2023Quote: Qdot-labeled Cel7A was prepared by mixing 3 nM biotinylated Cel7A with 2 nM Qdot 655 (Thermo Scientific; catalog number: Q10123MP) in 50 mM sodium acetate ...
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bioRxiv - Molecular Biology 2023Quote: ... Peptide mixtures were injected in 0.1% TFA into a C18 Acclaim PepMap100 trap-column (75 μm ID × 2 cm, 3 μm, 100Å, Thermo Fisher Scientific) for 3 minutes at 5 μL/min with 2% ACN ...
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bioRxiv - Neuroscience 2023Quote: ... the cell pellet was then resuspended in enzyme assay buffer again and sonicated at 3-second intervals for 2 minutes at 50% amplitude (Cat# FB120220, Fisher Scientific). The cell lysate was centrifuged at 1000 x g at 4°C for 10 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were typically passaged every 2-3 days by treating the cultures with 0.05% Trypsin-EDTA (Thermo Fisher Scientific, cat# 15400054) for 8-10 min at 37°C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Peptides were loaded first on a Trap column (Acclaim PepMap 100 3 µm C18 column of 2 cm length and 75 µm inner diameter, ThermoFisher Scientific) and then injected on an analytical column (EasySpray 15 cm column 2 µm 100 Å C18 column with 75 µm inner diameter ...
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bioRxiv - Biochemistry 2023Quote: ... High-resolution micrographs for single-particle reconstructions (Figure 2 and 3) were collected on a Titan Krios G4 (Thermo Fisher Scientific), which was operated at 300 kV acceleration voltage ...
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bioRxiv - Cancer Biology 2023Quote: ... The tumors were placed into a chilled conical tube containing 2-3 mL of TrypLE Express Enzyme (Gibco; cat. no. 1260413) and transported on ice to a biosafety hood ...
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Pan-tissue mitochondrial phenotyping reveals lower OXPHOS expression and function across tumor typesbioRxiv - Biochemistry 2023Quote: ... of 3 to 30% acetonitrile using a 2□μm EASY-Spray PepMap RSLC C18 75□μm × 250□mm column (Thermo Fisher) with a column temperature of 55□°C ...
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bioRxiv - Immunology 2023Quote: ... Serum starved PANC-1 and MiaPaCa-2 cells were incubated with human properdin (20 µg/ml) and CellEvent™ Caspase-3/7 Green Detection Reagent (Invitrogen) (5 μM ...
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bioRxiv - Cell Biology 2023Quote: ... slides were rehydrated with 25mM HEPES (Sytox Green) or dPBS (Hoechst, TOTO-3) for 2 minutes followed by incubation in 1µM Sytox Green (Invitrogen, Waltham, MA) in 25mM HEPES ...
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bioRxiv - Microbiology 2024Quote: ... 4 µl of peptide mixtures (1µg) were loaded onto an Acclaim PepMap precolumn (75 µm x 2 cm, 3 µm, 100 Å; Thermo Scientific) equilibrated in solvent A and separated at a constant flow rate of 250 nl/min on a PepMap RSLC C18 Easy-Spray column (75 µm x 50 cm ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were grown in cell culture dishes (Falcon) and passaged every 2-3 days via trypsinization with 0.0S% Trypsin-EDTA (2S300-0S4, Thermo Fisher Scientific) at 80-90% confluency ...
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bioRxiv - Biochemistry 2024Quote: ... which was equipped with a 75 μm x 2 cm trap column packed with C18 3 μm bulk resins (Acclaim PepMap 100, Thermo Scientific) and a 75 μm x 15 cm analytical column with C18 2 μm resins (Acclaim PepMap RSLC ...
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bioRxiv - Microbiology 2024Quote: ... the samples were stained with 80 μL CellEvent Caspase 3/7 Green detection Reagent 2 µM in PBS with 5% FBS (Invitrogen, C10423) and then incubated for 30 minutes at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... in 0.05 M TBS and then incubated in the dark for 2 h at room temperature with secondary antibodies: donkey anti-mouse Alexa Fluor 488 (3 µl/ml; Life Technologies) and donkey anti-rabbit Alexa Fluor 594 (5 µl/ml ...
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bioRxiv - Biochemistry 2024Quote: ... onto a nanotrap column (75 μm i.d. x 2 cm precolumn, packed with Acclaim PepMap100 C18, 3 μm, 100 Å; Thermo Fisher) using an EASY-nLC 1200 system (Thermo Fisher) ...
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bioRxiv - Genetics 2024Quote: 100,000 HEK293 cells in a 6-well plate were transfected with Antisense oligonucleotide harbouring 2’-O Me phosphorothioate modifications at each nucleotide position (Integrated technologies) using 3 ul of Lipofectamine 2000 (11668027, ThermoFisher Scientific) in one ml OPTIMEM I Reduced Serum Medium with no phenol red (11058021 ...
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bioRxiv - Synthetic Biology 2024Quote: ... Libraries were aliquoted in 10 mL (OD600 ∼ 0.2) and re-grown for 2-3 h to extract plasmids using the GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific) for sequencing and for subcloning into the expression strain ...
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bioRxiv - Molecular Biology 2022Quote: ... We ligated both with an insert:vector ratio of 1:4 using Ligase (Thermo Scientific; 1 h, 22 C). The ligation mix was purified (Zymo Research ...
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bioRxiv - Cell Biology 2020Quote: ... Vacuolar membranes were stained with 1 μL of 1 mg/mL solution of FM 4-64 (T3166; Invitrogen) in DMSO added at the beginning of incubation of cells in YPD medium ...
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bioRxiv - Cell Biology 2024Quote: ... AlexaFluor 488 anti-rabbit (1:1000, green) and 568 anti-mouse (1:1000, red) (both Invitrogen; Table 4) secondary antibodies were also diluted in 1% BSA and 10 % goat serum in TBS and incubated in the dark for 45 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... 4 μL Tris pH 6.5 1 M and 1 μL Proteinase K (20 mg/ mL, Thermo Fisher Scientific) and incubated shaking for 3 hours at 45°C ...
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bioRxiv - Bioengineering 2024Quote: ... Primary antibodies were incubated at 4°C overnight (Abcam, ab138501, 1:1,000; Thermo Fisher Scientific, AM4302, 1:5,000). Horseradish peroxidase-conjugated secondary antibodies were incubated for 1 hr at room temperature (Abcam ...
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bioRxiv - Neuroscience 2020Quote: ... Caspase-3 (1:5000 anti-Rbt; CST; cat # 9662S) ERO1-L (1:1000 anti-Rbt; Thermo Fisher Scientific; cat # 702709) and β-Actin (1:3000 anti-ms ...
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bioRxiv - Cancer Biology 2020Quote: Plasmids (1 μg of sense and antisense guide RNAs + 3 μg donor for knockins) were diluted in 1 ml OptiMem (Gibco) and 20 μl of polyethylenimine (PEI ...
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bioRxiv - Developmental Biology 2021Quote: Donor embryos were injected at the 1-cell stage with 3-5nL of 1% Dextran-FITC 10,000 MW (Molecular Probes) and 0.05% Phenol Red (to see the solution ...
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bioRxiv - Cancer Biology 2022Quote: ... AF6-88.5.5.3, #17-5958-82), CD119 (1:100, 2E2, #13-1191-82), Armenian Hamster IgG isotype (1:100, eBio299Arm, #13488881) (Invitrogen); CD45 (1:400 ...
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bioRxiv - Developmental Biology 2022Quote: Organoids were treated immediately after PEG embedding for 3 days with neural differentiation medium comprised of a 1:1 mixture of neurobasal medium (GIBCO) and DMEM/F12 (GIBCO) ...
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bioRxiv - Pathology 2020Quote: ... 6-dihydroxy-2,4,5,7-tetraio-dospiro (isobenzofuran-1(3H),9[9H] xanthan)-3:1 dipotassium salt (50 mg/kg in 0.9% saline, Fisher Scientific) was injected retro-orbitally before catalyzing vessel injury with a 540 nm laser ...
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bioRxiv - Molecular Biology 2020Quote: ... To 1/3 of the sample 1/10 of the recommended amount of spike-in (ERCC RNA spike-in mix, Ambion) was added ...
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bioRxiv - Microbiology 2020Quote: ... 1 μL (10μM) reverse primer (ldh4_R, 5’-AATCACAGCAGCCCCTTG-3’) and 1 μL (1 U/μL) Platinum Taq DNA polymerase (Invitrogen, 10966-018) in a 50 μL total reaction volume ...
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bioRxiv - Cell Biology 2022Quote: ... The secondary antibody in PBS supplemented with 3% BSA (Thermofisher, Mouse 800, 1:20000 and Thermofisher, Rabbit 680, 1:20000) was incubated in the dark for 1 hour on a rolling platform at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... Tenfold dilutions were used to infect confluent Vero E6 cells in a 96-well plate in MEM (5% FBS, 1% penicillin-streptomycin, 1% kanamycin, 3% amphotericin B (Gibco)) at 37°C and 5% CO2 ...
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bioRxiv - Molecular Biology 2024Quote: ... 1:3) (60) followed by secondary Alexa Fluor 488 goat α-mouse IgG antibody (Invitrogen / Thermo Fisher Scientific – 1:1,000).
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bioRxiv - Neuroscience 2023Quote: ... the brains were placed in the secondary antibody solution containing 1xPBS/0.1% Triton X-100/3% donkey serum and a 1:1000 dilution of donkey anti-sheep 647 (A-21448, Thermofisher) for 10 days.