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Citations for Thermo Fisher :
5001 - 5050 of 10000+ citations for 3 Cyclohex 1 enyl acrylic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... slides were incubated for 24 minutes in a mixture of 3% 3-Aminopropyltriethoxysilane (ACROS Organics, 430941000) and 5% acetic acid in HPLC EtOH ...
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bioRxiv - Immunology 2020Quote: ... 3 μL RT reaction mix consisting of 3 μL of 50 μM random hexamers (Thermo Fisher), 0.8 μL of 25 mM deoxyribonucleotide triphosphates (dNTPs ...
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bioRxiv - Molecular Biology 2022Quote: ... siM1-4: 5’ -ACTGGTAGCTTATTAAAGATT- 3’) and the HOXA1 siRNA (5’ - AGAACTTCAGTGCGCCTTATT- 3’) were purchased from Invitrogen™ (Silencer® Select siRNA ...
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bioRxiv - Microbiology 2024Quote: ... This was followed by adding TMB (3, 3, 5, 5′-tetramethylbenzidine) peroxidase substrate (Thermo Fisher Scientific) for about 10 min ...
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bioRxiv - Immunology 2023Quote: ... To assess active caspase 3 activity the CaspGLOW™ Fluorescein Active Caspase-3 Staining Kit (Invitrogen) was used ...
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bioRxiv - Cell Biology 2023Quote: ... Target mtDNA gene (Forward primer: 5′-CACCCAAGAACAGGGTTTGT-3′, Reverse primer: 5′-TGGCCATGGGTATGTTGTTAA-3′, Invitrogen custom primers) and reference 18S ribosomal RNA gene (Forward primer ...
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bioRxiv - Cancer Biology 2023Quote: ... slides were incubated for 24 min in a mixture of 3% 3-aminopropyltriethoxysilane (ACROS Organics, 430941000) and 5% acetic acid in HPLC ethanol ...
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bioRxiv - Biophysics 2024Quote: ... cells were stained with DiIC12(3) (1,1’-Didodecyl-3,3,3’,3’-Tetramethylindocarbocyanine Perchlorate) (DiI, Invitrogen cat # D383) for 10 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... 50 μg/mL streptomycin and 2 ng/mL mouse IL-3 (mIL-3, Gibco, Thermo Fisher). 32D cells were maintained in identical culture media with the exception of being supplemented with 15% WEHI-3B cell-conditioned media as a source of IL-3.
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bioRxiv - Biochemistry 2024Quote: ... 50 μg/mL streptomycin and 2 ng/mL mouse IL-3 (mIL-3, Gibco, Thermo Fisher). 32D cells were maintained in identical culture media with the exception of being supplemented with 15% WEHI-3B cell-conditioned media as a source of IL-3.
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bioRxiv - Cell Biology 2024Quote: ... and biotinylated at the 3’end with the Pierce RNA 3’end biotinylation kit (Thermo Fisher). Both antisense and sense strands of the DNA sequence were amplified to obtain a DNA template for in-vitro transcription (Supplementary Figure ...
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bioRxiv - Microbiology 2021Quote: ... then 40 cycles of 94°C for 1 minute and 60°C for 1 minute on a QuantStudio 3 real-time thermocycler (Thermo Fisher Scientific, Waltham, MA, USA). KPPR1 genomic DNA was used as positive control and 100% reference for calculating Klebsiella relative abundances ...
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bioRxiv - Cell Biology 2020Quote: ... using a 45 min gradient from 5-60% acetonitrile with 0.1% formic acid and directly sprayed via a nano-electrospray source in a quadrupole Orbitrap mass spectrometer (Q ExactiveTM, Thermo Fisher Scientific™) [81] ...
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bioRxiv - Cell Biology 2020Quote: Fluorescence ratios of pHLARE were first acquired in cell culture medium and then cells were sequentially incubated in a potassium-phosphate buffer (50 mM potassium phosphate, 80 mM potassium chloride, 1 mM magnesium chloride) containing 20 μM nigericin free acid (Thermo Fisher Scientific; N1495) at pH 6.5 and 5.0 ...
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bioRxiv - Molecular Biology 2020Quote: ... Zebrafish larvae were incubated in 1x E3-medium buffered with 4mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) (Life Technologies, Carlsbad, CA, USA). Seahorse XF Cell Mito Stress kit (Agilent Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... At the end with SYBR green (solution 1:10000 in PBS 1X, SYBRTM green I Nucleic Acid Gel Stain, INVITROGEN, Eugene, Oregon, USA) DNA was stained ...
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bioRxiv - Microbiology 2021Quote: ... The gel was stained for 30 min in 0.5× TBE buffer containing 1× SYBR Gold nucleic acid staining solution (Life Technologies, Grand Island, NY), and then ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were then incubated overnight with primary antibodies at 4°C in B/P buffer containing either 1:50 rabbit anti-fatty acid synthetase (MA5-14887; Invitrogen, Waltham, MA, USA) or 1:500 rabbit anti-peroxisome proliferator-activated receptor gamma (ab45036 ...
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bioRxiv - Biochemistry 2020Quote: ... strains were negatively selected against the URA3 marker gene using 1 mg/mL of 5-Fluoroorotic Acid (5-FOA) (Fisher Scientific, F10501-5.0) in SD-plates ...
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bioRxiv - Developmental Biology 2022Quote: ... Proteins on the beads were eluted with 300 μl DLB containing 2 mM biotin and precipitated by adding 1/10 volume of 8.74 M Trichloroacetic acid solution (TCA, Fisher Scientific, Cat# BP555-500). Precipitated proteins were washed once with 500 μl of 0.874 M TCA and four times with ice-cold acetone ...
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bioRxiv - Genetics 2020Quote: ... and in some cases day 5 (120 hours) by staining with SYBR Green 1 nucleic acid stain (Invitrogen, ThermoFisher Scientific, Eugene, OR, USA) at 1:2000 dilution in PBS/0.3% BSA for 20 minutes ...
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bioRxiv - Microbiology 2020Quote: ... The quality was assessed by running 5 μL of DNA template combined with 1 μL loading dye on a 0.3% agarose gel with 1 μL of 10,000x SYBR™ Gold nucleic acid gel stain (Invitrogen™, Cat. No. S11494) per 10 mL of gel at 3 V cm-1 for 90 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... Hydrogels with varying PEG-αMA wt% and DTT:MMP2-degradable crosslinker ratios (Table 1) were prepared with pH 8.0 N-2-hydroxyethylpiperaine-N-2-ethane sulfonic acid (HEPES; Thermo Fisher Scientific, Waltham, MA) as a solvent to allow base-catalyzed polymerization ...
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bioRxiv - Cell Biology 2022Quote: ... Rat2 fibroblasts were cultured in the same medium with an added 1 % (v/v) of 100X MEM Non-Essential Amino Acids Solution (Thermo Fisher Scientific, # 11140068). Cells were incubated at 37 °C and 5 % CO2 ...
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bioRxiv - Systems Biology 2023Quote: ... Followed by a quick rinse in distilled water and a 2-minute rinse in 1% acetic acid solution (Fisher Scientific, cat# A38S-500). After dehydration with Xylene ...
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bioRxiv - Genomics 2022Quote: ... 15 μL of DNA extract with 1 μL of loading dye was run on a 0.3% agarose gel with 1 μL of 10,000x SYBR™ Gold nucleic acid gel stain (Invitrogen™, Cat. No. S11494) per 10 mL of gel at 3 V cm-1 for 90 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... treated with 100 μl stain mix (PBS, 1mg/ml RNase, 1/1000 SYBR™ Green I Nucleic Acid Gel Stain, Thermo Fisher Scientific) at 37°C for 30 min ...
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bioRxiv - Microbiology 2023Quote: The wild-type ISG20 full coding sequence (amino acids 1–181) was cloned into the pPROEX HTa vector (Thermo Fisher Scientific, cat. 10711018) by standard molecular biology techniques ...
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bioRxiv - Cell Biology 2023Quote: ... Gels were fixed in 100 mL of fixing solution (50% methanol, 10% acetic acid in Optima™ LC/MS grade water [W6-1, Thermo Fisher Scientific]) at room temperature ...
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bioRxiv - Cell Biology 2023Quote: For fixed cell immunofluorescence experiments cells were seeded on acid-treated 12mm glass coverslips in a 24-well dish and fixed in -20oC methanol (Fisher Scientific, A412-1) for 3 minutes ...
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bioRxiv - Physiology 2023Quote: Fractions were resuspended in 0.1% (v/v) formic acid (solvent A) and the peptides were separated and analyzed using an EASY-nLC system (Thermo Fisher Scientific, USA) with a two-column system setup coupled to a Q-Exactive HF mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2023Quote: 10µL of the PCR product was used to visualise amplification via gel electrophoresis on a 1% Agarose gel containing SYBR™ Green I Nucleic Acid Gel Stain (Thermo Fisher Scientific). The rest of the PCR product was cleaned using Monarch PCR & DNA cleanup kit (Cat ...
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bioRxiv - Molecular Biology 2024Quote: ... MEM Non-Essential Amino Acids Solution (1/100) and 5% (v/v) heat inactivated Fetal Bovine Serum (FBS) (all supplements were from Invitrogen, (Paisley, Scotland, UK)) ...
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bioRxiv - Neuroscience 2024Quote: ... mitotic SH-SY5Y cells were differentiated into non-mitotic neuronal cells by the addition of 20 µM all-trans retinoic acid on day 1 (Molecular Probes/Invitrogen, #207340010). From day 1 onwards ...
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bioRxiv - Plant Biology 2024Quote: ... One tank was treated with a mock solution while the other tank was treated with a 1 M solution of 2-aminoisobutyric acid (AIB) (Fisher Scientific, Cat. AAA1302114) to a final concentration of 10 mM ...
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bioRxiv - Molecular Biology 2024Quote: ... 1% MEM vitamin solution (100x) (GibcoTM, 11120037), 1% MEM non-essential amino acids (100x) (GibcoTM, 11140035) and 1mM sodium pyruvate (Thermo Scientific™, 11360039) in a humidified incubator at 37°C containing 5% CO2.
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bioRxiv - Biophysics 2024Quote: ... 30 μL of the lysate was diluted in 500 μL of PBS-TM containing 1× protease inhibitor and aurintricarboxylic acid (250 μg/mL, Thermo Fisher Scientific, AC103060250). The mixture was pre-cleared with 40 μL of poly-T magnetic beads (NEB ...
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bioRxiv - Physiology 2024Quote: ... were aspirated using a 21-gauge needle attached to a 5 mL disposable syringe in the presence of 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES)-buffered TCM-199 medium (Gibco, Life Technologies, Milan, Italy) and 0.005% (w:v ...
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bioRxiv - Developmental Biology 2024Quote: ... The IVM medium consisted of 25 mM 4-(2-hydroxyethyl) piperazine-1-ethanesulfonic acid-buffered tissue culture medium 199 (M199; Gibco, Paisley, Scotland, UK) supplemented with 5% calf serum (CS ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 uM FluoZin-3 AM (Invitrogen) was add to dishes to stain cells for 1 hour and then was washed twice with PBS ...
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bioRxiv - Cell Biology 2020Quote: ... 3 washes of 5X SSCT (ThermoFisher Scientific 15557044 ...
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bioRxiv - Immunology 2021Quote: ... 3 mM MgCl2 (Thermo Fisher AM9530G), 20 mM Tris-HCl pH 7.5 (Thermo Fisher ...
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bioRxiv - Neuroscience 2020Quote: ... TO-PRO-3 (Thermo Fisher Scientific), or Hoechst 33258 in the blocking buffer for 60 min.
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bioRxiv - Neuroscience 2020Quote: ... 3 µl of Liptoectamine RNAimax (Invitrogen) was diluted in 150 µl RPMI media and mixed with 150 pmol of siRNA in 150 µl RPMI media and left for 5 minutes ...
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bioRxiv - Microbiology 2021Quote: ... 3% fetal bovine serum (Life Technologies) and 1% Penicillin/Streptomycin/L-glutamine (Life Technologies) ...
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bioRxiv - Neuroscience 2021Quote: ... and laminin (3 µg/ml; Invitrogen). Cells were allowed to adhere in humidified tissue culture incubator (5% CO2 ...
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bioRxiv - Neuroscience 2021Quote: ... and laminin (3 μg/ml; Invitrogen). For axotomy ...
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bioRxiv - Neuroscience 2021Quote: ... with 3% B27 (Invitrogen, 17504-001) and 1% Glutamax (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... Di-3-ANEPPDHQ (D36801 ThermoFisher Scientific): 4.0 mM in pure DMSO diluted 100 times for use ...
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bioRxiv - Neuroscience 2020Quote: ... TNF-α (BMS607-3, Thermofisher Scientific), C1q (LS-F55223-1 ...