Labshake search
Citations for Thermo Fisher :
4951 - 5000 of 5129 citations for TAGAP Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... in-solution tryptic digestion was performed as described38 followed by desalting with a Pierce Peptide Desalting Spin Columns per the manufacturer’s protocol (ThermoFisher Scientific, cat no. 89852) and the peptides were dried by vacuum centrifugation ...
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bioRxiv - Molecular Biology 2022Quote: ... polyclonal antibody (1:100) was raised against a synthetic peptide sequence and prepared for our laboratory commercially by ResGen (Invitrogen, Carlsbad, CA, USA), as described61 ...
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bioRxiv - Neuroscience 2024Quote: ... Attached to the UPLC was a peptide trap (100 μm × 200 mm, 100 Å pore size, 5 μm particle size, C18, Thermo Fisher Scientific) for online desalting and purification followed by a 25 cm C18 reversed-phase column (75 μm × 250 mm ...
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bioRxiv - Microbiology 2024Quote: ... For LC-MS/MS measurements 800 ng of peptides were analyzed on a Q- Exactive Plus mass spectrometer (Thermo Scientific, San Jose, CA) coupled to an EASY-nLCTM 1000 UHPLC system (Thermo Scientific) ...
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bioRxiv - Systems Biology 2024Quote: ... Peptide separation was achieved using a reversed-phase analytical EasySpray column (Acclaim PepMap RSLC C18, 0.075mm × 250mm, Thermo Fisher Scientific, Waltham, United States) with a 120 minute linear gradient of 4%–32% solvent B (0.1% TFA in 80% ACN ...
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bioRxiv - Pathology 2024Quote: ... Tandem mass tag (TMT)-based labeling of peptides was performed using the TMT10plex™ Isobaric Label Reagent Set (Thermo Fisher Scientific, Cat#90110) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... Peptides were loaded on an Acclaim PepMap 100 pre-column (100 µm x 2 cm, C18, 5 µm, 100 Å; Thermo Fisher Scientific) with 0.07% trifluoroacetic acid at a flow rate of 20 µL/min for 3 min ...
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bioRxiv - Plant Biology 2024Quote: ... Analytical separation of peptides was accomplished on an Acclaim PepMap RSLC column (75 µm x 50 cm, C18, 2 µm, 100 Å; Thermo Fisher Scientific) at a flow rate of 300 nL/min ...
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bioRxiv - Biochemistry 2023Quote: ... the generated peptides were analyzed either using Easy-nanoLC-1000 system coupled to a Q-Exactive Plus mass spectrometer (Thermo Fisher Scientific, Germany) with 160-minutes gradients (blood stages ...
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bioRxiv - Cell Biology 2024Quote: ... by purification from the hybridoma supernatant using custom FLAG peptide column (DYKDDDDK-GGSC (custom GL Biochem) coupled to SulfoLink resin (ThermoFisher Scientific, cat# 20402)) and confirmed by gel electrophoresis ...
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bioRxiv - Pathology 2023Quote: ... and the digested peptides were subjected to Nano-HPLC/ESI-ion trap-MS/MS analysis with a Q Exactive mass spectrometer (Thermo Fisher Scientific, US). Raw MS data files were processed and analyzed using Mascot2.2 (Matrix Science ...
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bioRxiv - Microbiology 2023Quote: LC-MS/MS Analysis was performed by injection of peptides into an Easy-nLC HPLC system coupled to an Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific, Bremen, Germany). Peptide samples were loaded onto a 75 µm x 2 cm Acclaim PepMap 100 C18 trap column (Thermo Scientific ...
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bioRxiv - Systems Biology 2023Quote: ... peptide concentration was determined by measuring absorption at 280 nm and 260nm using Nanodrop 1000 ND-1000 spectrophotometer (Thermo Fisher Scientific Inc, USA) and the Warburg-Christian method51 and adjusted to final concentration of 0.12 µg/µL ...
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bioRxiv - Plant Biology 2023Quote: ... Peptides were resolved on an EASY-Spray PepMap RSLC C18 column (Thermo Scientific, 25 cm x 150 μm ID, 2 μm, 55°C) with the following gradient profile delivered at 300 nl min-1 by a Dionex RSLCnano chromatography system (Thermo Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... The peptides were separated on a nanoElute nanoHPLC system (Bruker, Bremen, Germany) using a 5 mm trapping column (Thermofisher Scientific, Waltham, MA, USA) and an in-house packed C18 analytical column (15 cm × 75 µm ID ...
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bioRxiv - Cell Biology 2022Quote: ... Peptides were separated on a 15 cm column packed with Accucore150 resin (150 Å, 2.6mm C18 beads Thermo Fisher Scientific, San Jose, CA) using an 80 min acetonitrile gradient ...
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bioRxiv - Microbiology 2023Quote: ... For LC-MS analysis ∼ 200ng (measured by a NanoDrop at a wavelength of 215 nm) of the peptide was injected by an Ultimate 3000 RSLCnano UHPLC system (Thermo Scientific, Germering, Germany).
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bioRxiv - Microbiology 2023Quote: Peptides were dissolved in 6 μL water containing 0.1% FA and 3% ACN and then 200ng/μL (measured by a NanoDrop at a wavelength of 215 nm) of the peptide was injected by an Ultimate 3000 RSLCnano UHPLC system (Thermo Scientific, Germeringen, Germany). Following injection ...
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bioRxiv - Cell Biology 2023Quote: ... C-terminal peptides of α-tubulin were analyzed by liquid chromatography (LC)-MS/MS (PROXEON coupled to a QExactive mass spectrometer, Thermo Fisher Scientific). Samples were searched against the UniProtKB Drosophila database using Pipeline ...
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bioRxiv - Biochemistry 2023Quote: ... The digested peptides were desalted using Oasis HLB cartridges (Waters, Milford, MA, USA) and dried by vacuum evaporation (Thermo Fisher Scientific, Bremen, Germany). The peptide concentration was measured using the BCA method.
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bioRxiv - Biochemistry 2023Quote: ... Peptides were separated on a PepMap RSLC C18 easy spray column (2 μm, 100 Å, 75 μm x 50 cm, Thermo Fisher Scientific) with an inner diameter of 75 μm and a column temperature of 40 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Mass spectrometric analysis of the peptide mixtures was performed on Ultimate 3000 RSLC nano system coupled with a Tribrid Orbitrap Eclipse (Thermo Fisher Scientific, USA). 1μg of the sample was loaded on Acclaim PepMap 75 μm x 2 cm C18 guard column (3μm particle size) ...
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bioRxiv - Immunology 2023Quote: ... The resulting peptides were extracted and analyzed by high-sensitivity LC-MS/MS on an Orbitrap Fusion mass spectrometer (Thermo Scientific, Waltham MA). Proteins were identified by database searching of the fragment spectra against the SwissProt (EBI ...
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bioRxiv - Biochemistry 2023Quote: ... Separated peptides were analyzed on-line via nanospray ionization into an Orbitrap Fusion™ Lumos™ Tribid™ mass spectrometer (Thermo Fisher Scientific) using the following parameters for MS1 data acquisition ...
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bioRxiv - Biochemistry 2023Quote: ... Peptides were separated on a PepMap RSLC C18 easy spray column (2 µm, 100 Å, 75 µm x 50 cm, Thermo Fisher Scientific) with an inner diameter of 75 µm ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting peptides were extracted from the gel pieces and analyzed using an Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific, Waltham, MA) in combination with an UltiMate 3000 RSLC nanoflow HPLC (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... The peptides were then resolubilized in 30 μL of 50 mM TEAB pH 8.5 and assayed using the PierceTM Quantitative Fluorometric Peptide Assay (Thermo Fisher Scientific, Waltham, MA). Fifty micrograms of peptides/sample were labeled with TMT labels (16-plex ...
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bioRxiv - Physiology 2023Quote: Fractions were resuspended in 0.1% (v/v) formic acid (solvent A) and the peptides were separated and analyzed using an EASY-nLC system (Thermo Fisher Scientific, USA) with a two-column system setup coupled to a Q-Exactive HF mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... the fractionated peptides spiked with iRT were loaded onto a pre-column (3 μm, 100 Å, 20 mm × 75 mm i.d., Thermo Fisher Scientific, USA) using a Thermo Scientific UltiMateTM 3000 RSLCnano LC a U3000 LC system ...
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bioRxiv - Cell Biology 2023Quote: ... Tryptic peptides were loaded onto a trap column (Acclaim™ PepMap™ 100 C18, 3μm, 5 × 0.3 mm, Fisher Scientific, San Jose, CA) at a flow rate of 10 μL/min using 2% acetonitrile in 0.1% TFA as loading buffer ...
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bioRxiv - Biochemistry 2023Quote: ... at ∼1ug/uL peptide concentration for liquid chromatography-tandem mass spectrometry (LC-MS) analysis on Dionex Ultimate 3000 RSLCnano system (Thermo Fisher Scientific, Inc) and Orbitrap Eclipse Tribrid MS (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Concentrations of proteins and peptides were calculated from the UV absorption at 280 nm using a Nanodrop spectrophotometer (Thermo Fisher Scientific, DE; USA).
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bioRxiv - Cancer Biology 2023Quote: ... Eluting peptides were ionized at a potential of +2 kV and introduced into a Q Exactive HF mass spectrometer (Thermo Fisher, Bremen, Germany). Intact masses were measured in the orbitrap with a resolution of 120,000 (at m/z 200 ...
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bioRxiv - Microbiology 2023Quote: Peptides were dissolved in 6 μL water containing 0.1% FA and 3% ACN and then 200ng/μl (measured by a NanoDrop at a wavelength of 215 nm) of the peptide was injected by an Ultimate 3000 RSLCnano UHPLC system (Thermo Scientific, Germeringen, Germany). Following injection ...
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bioRxiv - Molecular Biology 2024Quote: ... Peptides were analyzed by liquid chromatography–tandem mass spectrometry (LC-MS/MS) on an Orbitrap Fusion Lumos mass spectrometer (ThermoFisher Scientific, MA, USA) 54 ...
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bioRxiv - Biochemistry 2024Quote: ... 1 μg of peptide from each sample was taken and analyzed by mass spectrometry using an EASY-nLC1200 chromatography system (Thermo Fisher Scientific, USA) and an Orbitrap Fusion Lumos Tribrid mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... The Eluting peptides from the column were analyzed on a quadrupole-ion trap-Orbitrap QExactive Plus mass spectrometer (Thermo Scientific, San Jose, CA). Orbitrap survey MS1 scans from 375 m/z to 1500 m/z were performed at a resolving power of 70,000 at 200 m/z with an AGC target of 3×106 ions and maximum injection time set to 100 ms ...
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bioRxiv - Biochemistry 2024Quote: ... A total of 1 µg of peptides was loaded onto the Acclaim PepMap nano-trap column (C18, 100 Å, 75 μm × 2 cm, Thermo Fisher Scientific) with an isocratic flow of 6 µL/min of 2% ACN containing 0.05% TFA for 6 min ...
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bioRxiv - Cell Biology 2024Quote: ... Peptides were trapped on a C18 Acclaim PepMap 100 (5 μm, 300 μm x 5 mm) trap column (ThermoFisher Scientific, San Jose, USA) and eluted onto a C18 Acclaim PepMap100 3 μm ...
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bioRxiv - Molecular Biology 2024Quote: ... and 3 µg peptides of each sample was injected for LC-MS/MS analysis on an Ultimate 3000 RSLC nanoLC (Thermo Scientific, Bremen, Germany) in-line connected to an LTQ-Orbitrap Elite (Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2024Quote: ... Peptides were then desalted using SDB-XC Empore disk membranes in StageTips (GL Sciences, Tokyo, Japan) and measured with the Pierce™ Quantitative Colorimetric Peptide Assay (Thermo Fisher Scientific). Equal quantities of purified peptides from each sample were then dried and prepared for TMT labeling ...
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bioRxiv - Synthetic Biology 2024Quote: ... Peptide analysis was conducted using an Orbitrap Fusion Lumos Tribrid mass spectrometer (quadrupole-ion trap-Orbitrap, Thermo Fisher Scientific, San Jose, CA, USA), coupled with an Ultimate 3000 nanoLC system (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... The resulting peptides were desalted and subsequently analysed on UltiMate™ 3000 RSLCnano System connected to LTQ Orbitrap Velos mass spectrometer (Thermo Fisher Scientific).
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bioRxiv - Developmental Biology 2021Quote: ... Then embryos were incubated in the blocking solution for at least 4 hours and incubated overnight at 4°C with secondary antibodies anti-chicken-AF488 (Invitrogen, A11039, used at 1/800), anti-rabbit-HRP (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Coverslips were incubated in blocking buffer with fluorescent secondary antibodies (Donkey α-Rabbit 488 or Donkey α-Rabbit 546, ThermoFisher A-21206 or A-10040) for 4 hours at room temperature in the dark without agitation ...
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bioRxiv - Cancer Biology 2022Quote: ... and stained with secondary antibodies (donkey anti-rat-AF488 [A212008, Invitrogen] and donkey anti-goat AF647 [A21447, Invitrogen] diluted at 1:400 in blocking buffer) for 2 days at 4°C with shaking ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed with phosphate buffered Tween (PBS-T) and non-specific binding was blocked with TBS Startblock blocking buffer (Thermo Fisher Scientific, Rochester, NY, USA). Serum samples were serially diluted 3-fold starting with a 1:100 dilution and BAL and nasal wash samples were serially diluted 2-fold starting with a 1:2 dilution ...
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bioRxiv - Neuroscience 2019Quote: ... the neurons were incubated with goat anti-rabbit IgG antibody conjugated with Alexa Fluor 488 dye (A-11070, Life Technologies; 1000x dilution in blocking solution), and anti-mouse IgG antibody conjugated with Alexa Fluor 568 dye (A-11019 ...
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Evolutionarily divergent mTOR remodels the translatome to drive rapid wound closure and regenerationbioRxiv - Developmental Biology 2021Quote: ... Slides were washed three times 15 min in blocking buffer and incubated with secondary antibody conjugated with Alexa Fluor 488 or Alexa Fluor 568 (Thermo Fisher, A-11017 or A10037) at 1:500 dilution and DAPI at 1:1000 dilution in blocking buffer for 1h in the dark at RT ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were washed 3 times with blocking solution followed by incubation with Alexa 488-conjugated goat anti-rabbit IgG (H+L) (Life Technologies:# A-11034; 1:5000) for 2.5 h ...