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Citations for Thermo Fisher :
4951 - 5000 of 6821 citations for Recombinant Human IL1RL1 None tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Cells were washed twice with FACS buffer and stained with goat anti-Human IgG Alexa Fluor 647 (Invitrogen) or goat anti-Mouse IgG Alexa Fluor 647 (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... and F(ab’)2-Goat anti-Human IgG-Fc secondary antibody conjugated with R-phycoerythrin (ThermoFisher Catalog # H10104). Labeled cells were acquired using a Thermo-Fisher Attune NxT flow cytometer and analyzed using Flowjo Software.
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bioRxiv - Microbiology 2020Quote: ... Human embryonic kidney (HEK) 293T cells (ATCC CRL-3216) were cultured in DMEM+GlutaMAX-I (Thermo Fisher Scientific) supplemented with heat-inactivated 8% FBS.
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bioRxiv - Immunology 2021Quote: ... Intracellular staining was performed using buffer sets of Human Regulatory T-cell Staining Kit (eBioscience/Thermo Fisher Scientific), following the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... and TLR4 on the beads were stained with mouse monoclonal APC anti-human TLR4 antibody (clone HTA125; ThermoFisher) for 1 h on ice prior to flow cytometric analysis ...
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bioRxiv - Immunology 2020Quote: ... 1 mM EDTA.) with the following anti human antibodies: anti CD3-APC-eFluor 780 (Invitrogen, 47-0037-41), anti CD14-APC-eFluor 780 (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... HEK-293T overexpressing the human ACE2 were kindly provided by Integral Molecular Company and maintained in DMEM (Invitrogen) with 10% fetal bovine serum ...
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bioRxiv - Molecular Biology 2020Quote: SV40-transformed normal (MRC5-V1) and XPV (XP30RO) human fibroblasts were grown in Minimum Eagle Medium (MEM, Gibco) supplemented with 10% fetal calf serum ...
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bioRxiv - Microbiology 2021Quote: The human hepatocellular carcinoma Huh7 cell line (CLS 300156) was cultured in Dulbecco’s modified eagle medium (DMEM, Gibco) with glutamax supplemented with 10% FBS ...
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bioRxiv - Immunology 2020Quote: ... Labelled cDNA was hybridised to an Affymetrix GeneChip® Human Transcriptome Array 2.0 (Applied Biosystems, Thermo Fisher Scientific). Raw intensity files (CEL files ...
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bioRxiv - Immunology 2020Quote: ... Labelled cDNA was hybridised to an Affymetrix GeneChip® Human Transcriptome Array 2.0 (Applied Biosystems, Thermo Fisher Scientific). Raw intensity files (CEL files ...
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bioRxiv - Microbiology 2021Quote: Human primary fibroblasts (HFFs, ATCC® CCL-171™) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Invitrogen) supplemented with 10% heat inactivated Fetal Bovine Serum (FBS ...
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bioRxiv - Immunology 2020Quote: ... Positive proliferation control wells were set up by plating 1 Dynabeads Human T-Activator CD3/CD28 (ThermoFisher Scientific) for every 200 T-cells ...
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bioRxiv - Immunology 2021Quote: Isolation of CD3+ T-cells was performed using Dynabeads® Untouched™ Human T-cells Kit (11344D, Invitrogen). CD3+ T-cells were stained with CellTrace Violet Cell Proliferation Kit (C34571 ...
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bioRxiv - Immunology 2020Quote: ... BM14 (ECACC 88052033) human HLA-Typed Lymphoblastoid Cell Line was obtained from ECACC and maintained in RPMI (ThermoFisher) media supplemented with 10% FBS in the absence of antibiotics ...
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bioRxiv - Immunology 2021Quote: ... The human multiple myeloma cell line XG-1 was cultured in Iscove modified Dulbecco medium (Invitrogen Life Technologies) containing 10% fetal bovine serum ...
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bioRxiv - Immunology 2021Quote: ... The human multiple myeloma cell line XG-1 was cultured in Iscove modified Dulbecco medium (Invitrogen Life Technologies) containing 10% fetal bovine serum ...
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bioRxiv - Genomics 2021Quote: ... Primary human T cells were cultured for up to 20 days in ATCC-modified RPMI (Thermo Fisher, #A1049101) supplemented with 10% FBS ...
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bioRxiv - Immunology 2021Quote: ... T cells were activated using a 1:1 ratio of DynaBeads Human T-Activator CD3/CD28 (Thermo Fisher) for 24 hours ...
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bioRxiv - Microbiology 2021Quote: ... Human foreskin fibroblast (HFF) cells were grown and maintained in DMEM containing 4.5g/L glucose (Gibco 11965-092), supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Molecular Biology 2021Quote: HEK293F is a female human embryonic kidney cell line transformed and adapted to grow in suspension (Life Technologies). HEK293F cells were grown in 293FreeStyle expression medium (Life Technologies) ...
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bioRxiv - Cell Biology 2021Quote: ... Alpha-actinin-1-M-sstFRET containing the sstFRET module inserted between 300aa and 301aa within the first spectrin repeat of human alpha-actinin-1 (P12814.2) was in the neomyocin selectable pcDNA3.1 expression vector (Invitrogen/ThermoFisher). Actinin-C-sstFRET containing the sstFRET module added to the C-terminus of human alpha-actinin-1 (P12814.2 ...
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bioRxiv - Immunology 2022Quote: ... Human Raji (Burkitt’s lymphoma) and SKOV3 (ovarian carcinoma) were cultured in complete RMPI (Roswell Park Memorial Institute, Gibco) and DMEM (Dulbecco’s Modified Eagle’s Medium ...
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bioRxiv - Immunology 2022Quote: ... podocytes were treated with patient sera in the presence of a control antibody (human IgG, Invitrogen #02-7102) or anti uPAR antibody (2G10 ...
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bioRxiv - Biophysics 2022Quote: ... Poly-L-lysine coated chambers were then incubated with anti-human CD3 antibody (HIT3a clone, Thermo Fisher Scientific) in PBS at a concentration of 10 µg/mL for 2 hours at 37°C or overnight at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... cells and MDA-MB-231 human breast cancer cells were obtained from ATCC and cultured in Dulbecco’s Modified Eagle’s Medium (DMEM; Gibco) with reduced glucose (1 g/L) ...
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bioRxiv - Cancer Biology 2022Quote: ... T cells were isolated from PBMCs using the Dynabeads® Untouched Human T Cells Kit (Invitrogen, Karlsruhe, Germany) following the manufacturer’s protocol.
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bioRxiv - Biochemistry 2022Quote: ... labeled with Alexa Fluor 488 from the Zenon™ Human IgG Labeling kit (Molecular Probes, Eugene, OR, USA) and 15 μg/mL of 7-amino-actinomycin D (7-AAD ...
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bioRxiv - Biophysics 2022Quote: ... designated as ‘Cav1.2(ΔC)’) construct was obtained from a full-length human Cav1.2 α1 (Uniprot Q13936-20) template in pcDNA3.1 (Invitrogen) using a Gibson Assembly kit (NEB) ...
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bioRxiv - Biochemistry 2022Quote: ... Histidine rich glycoprotein (HRG) was purified from human plasma with a cobalt-loaded resin (Thermo Scientific, Waltham, America) using IMAC-based enrichment (56) ...
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bioRxiv - Cancer Biology 2022Quote: ... 50 μl each) using the Immune Monitoring 65-Plex Human ProcartaPlex Panel (ThermoFisher, Catalog number: EPX650-16500-901), as per manufacturer instructions ...
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bioRxiv - Cell Biology 2022Quote: ... and aged (>60 years) human primary keratinocytes (HPK) were isolated with trypsin (Gibco, Life Technologies, Carlsbad, CA, USA) and dispase (Dispase II ...
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bioRxiv - Cell Biology 2022Quote: ... and aged (>60 years) human primary keratinocytes (HPK) were isolated with trypsin (Gibco, Life Technologies, Carlsbad, CA, USA) and dispase (Dispase II ...
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bioRxiv - Cell Biology 2022Quote: Human acute monocytic leukemia THP-1 (ATCC, TIB-202™) cells were cultured in RPMI-1640 medium (Gibco) supplemented with heat-inactivated 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2022Quote: ... Human IFNg and IL-13 cytokine secretion was measured by ELISA using eBioscience Ready-SET-Go! (now ThermoFisher) ELISA kits according to the manufacturer’s protocol.
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bioRxiv - Immunology 2022Quote: ... Gene expression levels were obtained using Human Clariom S arrays on GeneTitan MC Instrument International (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2022Quote: ... and the human epithelial cell line A549 (ATCC No. CCL-185) were cultivated in Dulbecco’s modified Eagle’s medium (DMEM; Gibco BRL Life Technologies ...
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bioRxiv - Microbiology 2022Quote: ... falciparum was maintained at 4% haematocrit in human RBCs in AlbumaxII media (RPMI-1640, 25 mM HEPES (Gibco), 367 μM hypoxanthine (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2022Quote: ... human embryonic kidney (HEK) 293T and Vero cells (ATCC) were maintained with Dulbecco’s Modified Eagle’s medium (DMEM) (Gibco), supplemented with 10% fetal calf sera (FCS ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... cells stably expressing human M4 mAChR or mutant constructs were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Invitrogen) containing 5% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2022Quote: Human BDNF-biotin (50 nM) was incubated with 50 nM Qdot-605 Streptavidin conjugate (Thermo Fisher Scientific; Q10101MP) on ice for 1 h in Neurobasal medium to facilitate the biotin-streptavidin interaction ...
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bioRxiv - Molecular Biology 2023Quote: Human retinal epithelial cells (hTERT-RPE1, ATCC) were grown in 10% (v/v) FBS (Atlas) / DMEM (Thermo Scientific). Media was supplemented with 1 mM L-glutamine (Gibco ...
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bioRxiv - Microbiology 2023Quote: Human primary fibroblasts (HFFs, ATCC® CCL-171™) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2022Quote: ... and human cervical cancer cells (HeLa) were maintained in Dulbecco’s modified Eagle’s (DMEM, 11965092, GIBCO, Carlsbad, CA, USA) medium ...
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bioRxiv - Immunology 2022Quote: ... and CD4+ and CD8+ T cells were further isolated with Dynabeads Human CD4+ or CD8+ Isolation Kits (ThermoFisher), following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... the membrane was incubated with primary antibody against aSyn (Monoclonal anti-human mouse α-synuclein Syn211 Invitrogen™), against A11 oligomers (Polyclonal Rabbit Anti-amyloid oligomer A11 AB9234 Millipore™ ...
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bioRxiv - Neuroscience 2022Quote: ... cells were changed to EC medium consisting of human endothelial serum-free media (hESFM; Thermo Fisher Scientific #11111044), 0.25X B27 supplement (Fisher Scientific #17-504-044) ...
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bioRxiv - Microbiology 2022Quote: ... mouse fibroblast cell lines and HEK293T human cell lines (ATCC CRL-3216) were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Gibco) with 10% fetal calf serum (FBS ...
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bioRxiv - Cell Biology 2022Quote: Two separate human ASAH1-siRNAs (50 nM) (Dharmacon,) were transfected into 621-101 cells using Lipofectamine RNAiMAX (Invitrogen) according to the manufacturer’s protocols ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Total RNA was extracted from mouse skin tissue or mouse/human EpSCs using Trizol reagent (Invitrogen, Massachusetts, USA), and used as a template for cDNA synthesize using HiScript II reverse transcriptase (Vazyme Biotech ...