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Citations for Thermo Fisher :
451 - 500 of 10000+ citations for Saint Louis Encephalitis Virus NS1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... 12 µL of SARS-CoV-2 stock virus (USA_WA1/2020; 8.3×104 pfu) in OptiMEM supplemented with 1x antibiotic/antimycotic (Gibco) was pipetted directly onto each surface being tested and spread with a pipette tip to facilitate efficient drying ...
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bioRxiv - Microbiology 2021Quote: ... Transmissible gastroenteritis virus [TGEV] and Swine deltacoronavirus [SDCoV] (VetMAX™ PEDV/TGEV/SDCoV, Thermo Fisher Scientific, MA, USA) and Senecavirus A [SVA] (EXOone Seneca Virus Valley ...
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bioRxiv - Microbiology 2021Quote: ... The spike of the SARS-CoV-2.SctΔ19 B.1.617.2 (delta) virus was generated (GeneArt Gene Synthesis, ThermoFisher Scientific) from the full protein sequence of the original SARS-CoV-2 isolate Wuhan-Hu-1 (WH1 ...
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bioRxiv - Immunology 2020Quote: ... Reverse transcription of isolated RNA was performed using a Moloney murine leukemia virus-reverse transcriptase kit (Life Technologies) and oligo(dT ...
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bioRxiv - Synthetic Biology 2022Quote: ... undiluted SINV-containing supernatants were combined with the TaqMan™ Fast Virus 1-Step Master Mix (ThermoFisher, #4444434) and the appropriate primer-probe sets in technical triplicates ...
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bioRxiv - Microbiology 2022Quote: ... Premixes were prepared for each amplification reaction using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific), according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2022Quote: ... C and S genes of the Hepatitis B virus (accession number: KY00323035) was ordered from GeneArt (ThermoFisher Scientific) and is available from Addgene (Addgene # 179155).
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bioRxiv - Neuroscience 2023Quote: ... C-Myc) introduced by Sendai virus according to the manufacturer’s instructions (Cytotune iPS 2.0 Sendai reprogramming kit, ThermoFisher) or by using an episomal method ...
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bioRxiv - Cell Biology 2023Quote: ... After a brief culture to expand the erythroblast population a Sendai virus based approach (CytoTune 2.0 Kit, Invitrogen) was used to express the Yamanaka factors without any genomic integration (27) ...
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bioRxiv - Microbiology 2023Quote: ... The cell and virus lysates were Western blotted and probed with a goat polyclonal anti-gp120 antibody (Invitrogen), the 4E10 anti-gp41 antibody (NIH HIV Reagent Program ...
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bioRxiv - Biochemistry 2023Quote: Bacmid preparation and virus production were performed according to the Bac-to-Bac baculovirus system manual (Gibco, Invitrogen). Spodoptera frugiperda Sf9 cells at a density of 3 × 106 cells/ml were co-infected with baculoviruses encoding receptor and Gi trimer at the ratio of 1:1 ...
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bioRxiv - Biochemistry 2023Quote: Bacmid preparation and virus production were performed according to the Bac-to-Bac baculovirus system manual (Gibco, Invitrogen). Spodoptera frugiperda Sf9 cells at a density of 3 × 106 cells/ml were co-infected with baculoviruses encoding receptor and Gi trimer at the ratio of 1:1 ...
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bioRxiv - Cancer Biology 2023Quote: ... Epstein–Barr virus-transformed lymphoblasts were obtained from Coriell Cell Repositories and were grown in RPMI 1640 (Gibco) supplemented with 10% FBS and penicillin/streptomycin (Gibco) ...
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bioRxiv - Microbiology 2023Quote: ... l ml of the seed virus was treated with 2 μl TURBO DNase (Thermo Fisher Scientific, Cat# AM2238) and incubated at 37°C for l h ...
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bioRxiv - Immunology 2023Quote: ... Stock virus was sub-passaged through Madin-Darby canine kidney (MDCK) cells in Dulbecco’s modified Eagle’s medium (DMEM; Gibco), harvested as tissue culture supernatant and viral titres determined by cytopathic effects on MDCK cells and expressed as the mean log10 tissue culture infective dose required to kill 50% of the cells (TCID50 ...
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bioRxiv - Neuroscience 2023Quote: ... cDNA synthesis and PCR were performed using TaqMan™ Fast Virus 1-Step Multiplex Master Mix (#4331182; ThermoFisher). Probes were used to detect the mouse transcripts of Htr2a and Gapdh (Mm00555764_m1 and Mm99999915_g1 4448892 ...
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bioRxiv - Cell Biology 2023Quote: ... infected with Sendai virus containing Yamanaka factors from the CytoTune™-iPS 2.0 Sendai Reprogramming Kit (ThermoFisher, A165167) according to manufacturer recommendations ...
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bioRxiv - Biochemistry 2023Quote: ... 8 ml P4 virus was used to infect 800 ml cultures of Expi293F GnTI-Cells (Thermo Fisher Scientific) cells at 2-3 × 106 cells supplemented with 1% Fetal Bovine Serum (FBS) ...
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bioRxiv - Immunology 2024Quote: ... in virus growth medium (1X MEM [Gibco 11095080], 5% FBS [Hyclone SH30070.03HI] and 1% Penn-Strep [Gibco 10378016]). Monoclonal antibody dilution plates were prepared similarly to serum dilution plates ...
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bioRxiv - Neuroscience 2024Quote: Human fibroblast cells were reprogrammed using the Sendai virus-based CytoTune iPS 2.0 kit (CytoTune 2.0, Invitrogen, A16517). Fibroblasts were plated in six-well plates two days before transduction to achieve a density of 2 × 105 to 3 × 105 cells per well ...
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bioRxiv - Microbiology 2024Quote: ... the cells were incubated with 10-fold serial dilutions of the virus for 1 h and overlaid with 0.55% methylcellulose or 1.0% cellulose in minimal essential medium (MEM) (Gibco) supplemented with 2% FBS (Corning ...
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bioRxiv - Microbiology 2024Quote: ... and 2 U/µl of M-MulV-RT (Reverse transcriptase of Moloney Murine Leukemia Virus; Thermo Scientific, USA).
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bioRxiv - Genomics 2024Quote: ... Virus was produced by transfecting HEK293T cells with the plasmid and the Lipofectamine 3000 kit (Thermo Fisher Scientific). U87MG were transduced with the supernatant containing the NY-ESO-1 virus ...
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bioRxiv - Microbiology 2024Quote: ... and CC81 (feline cell line transformed by mouse sarcoma virus ECACC 90031403) cells were grown in Dulbecco’s modified Eagle’s medium (DMEM)-high glucose (GlutaMAX, Gibco) supplemented with 1% penicillin/streptomycin and 10% fetal bovine serum (Gibco ...
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bioRxiv - Bioengineering 2024Quote: ... Virus production medium contained DMEM supplemented with L-glutamine and sodium pyruvate and 10% heat-inactivated FBS (Gibco). Viral supernatants collected roughly 48- and 72-hours post-transfection were sterile-filtered through a 0.45-micron filter and concentrated in 100kDa molecular weight cutoff filters.
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bioRxiv - Immunology 2022Quote: Protein-protein crosslinking using BMOE (Thermo Scientific) was carried out according to the product manual ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein A and Protein G Dynabeads (Invitrogen) were incubated for 6 hours at 4°C with 5 μg H3K27me3 (07-449 ...
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bioRxiv - Plant Biology 2023Quote: ... Protein A and Protein G Dynabeads (Invitrogen) were added and incubated at 4 °C for 2 hours with rotation ...
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bioRxiv - Immunology 2022Quote: ... Protein A or protein G Dynabeads (Invitrogen) were prepared according to manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... Protein A and Protein G Dynabeads (Invitrogen) premixed at 1:1 ratio were used for pre-clearing for 1 hour at 4°C ...
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bioRxiv - Biochemistry 2023Quote: Protein-protein crosslinking using BMOE (Thermo Scientific) was carried out according to the product manual ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Quality and quantity of recovered high molecular weight DNA was assessed by electrophoresis on agarose gel, by spectrophotometry (Nanodrop, Saint-Remy-lés-Chevreuse, France) and with the QuBit fluorometric quantitation system (Life Technologies, Villebon-sur-Yvette, France).
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bioRxiv - Plant Biology 2021Quote: ... The final concentration of DNA samples was measured using Qubit 2.0 Fluorometer dsDNA BR Assay kits (Life Technologies-Thermo Fisher Scientific, Saint Aubin, France) and the final fragment size distribution using an Agilent 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... and on a UPLC Waters Acquity (Waters Corp, Saint-Quentin-en-Yvelines, France) coupled to Q-Exactive mass spectrometer (Thermo Scientific, San Jose, CA). Experimental settings for global approach by LC-HRMS were carried out as detailed in the paper of Garali et al [19].
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bioRxiv - Neuroscience 2023Quote: Metabolite profiling analysis was carried out on an Acquity UPLC system (Waters Corp, Saint-Quentin-en-Yvelines, France) coupled to a hybrid Orbitrap- and Q Exactive-based instrument (Thermo Fisher Scientific, Illkirch, France). To analyze and compare the microglial cell metabolome under different inflammatory conditions ...
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bioRxiv - Immunology 2020Quote: ... Precipitated proteins were bound to 50:50 Protein A: Protein G Dynabeads (Invitrogen) for one to two hours and washed extensively with IP wash buffer (50 mM Tris pH 8 ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were incubated with fast-flow protein G- or protein A-Dynabeads (Invitrogen) for 2 hours and centrifuged to eliminate nonspecifically bound proteins ...
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bioRxiv - Microbiology 2020Quote: Concentrated live or inactivated virus samples were mixed with Novex™ Tris-Glycine SDS Sample Buffer (2X) (Thermofisher Scientific) with NuPAGE™ Sample Reducing Agent (10X ...
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bioRxiv - Neuroscience 2020Quote: ... patient-derived fibroblasts were infected with Sendai virus using the CytoTune-iPS 2.0 Sendai Reprogramming Kit (Invitrogen, CA, US) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2021Quote: ... RNA was reverse transcribed and amplified using TaqMan Fast Virus 1-Step Master Mix qRT-PCR Master Mix (Invitrogen) on the LightCycler 480 or LC96 instrument (Roche ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Virus titres were measured from a thawed aliquot by: (1) mixing 20 μl with 200 μl of TRIzol (Invitrogen); (2 ...
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bioRxiv - Neuroscience 2019Quote: ... PBMCs were transduced with 2.0 Sendai virus particles containing four Yamanaka factors using the CytoTune-iPS Sendai Reprogramming Kit (ThermoFisher). After seven days ...
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bioRxiv - Microbiology 2019Quote: ... Initial virus was recovered from T7 transcribed RNA using reverse transfection of Huh7.5.1 cells by Lipofectamine® 2000 (Invitrogen) (for virus rescue ...
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bioRxiv - Microbiology 2019Quote: ... Two microliters of extracted RNA was amplified using TaqMan™ Fast Virus 1-Step Master Mix (Thermo Fisher Scientific) according to the manufacturer’s protocol and primers specific for HCoV-229E [30] or RSV-B [31] ...
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bioRxiv - Microbiology 2021Quote: ... Virus prepared for purification by ultracentrifugation was grown in serum-free media (VP-SFM; Gibco, ThermoFisher Scientific, Waltham, MA) supplemented with 1% (v/v ...
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bioRxiv - Microbiology 2021Quote: ... Virus prepared for purification by ultracentrifugation was grown in serum-free media (VP-SFM; Gibco, ThermoFisher Scientific, Waltham, MA) supplemented with 1% (v/v ...
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bioRxiv - Microbiology 2021Quote: ... a homolog of the vaccinia virus H3L gene) [13] using the Path-ID™ qPCR Master Mix (Thermofisher Scientific). Nucleic acid from capripoxvirus positive samples was further analysed by a qPCR targeting LSDV011 designed to differentiate the three capripoxvirus species [14] and a qPCR targeting LSDV008 designed to differentiate LSDV wildtype strains from Neethling vaccine strains [15] ...
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bioRxiv - Immunology 2021Quote: ... RT-qPCR reactions were performed with Taq-Man™ Fast virus 1-step master mix (ThermoFisher Scientific, Cat. 4444436) and primer-probe sets targeting SARS-CoV-2 nucleocapsid (N1 set ...
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bioRxiv - Microbiology 2020Quote: ... Virus stocks were generated by transfecting the clone into Vero cells using Lipofectamine 2000 (Thermo Fisher, cat# 11668-019), and harvested at various times post-transfection.
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bioRxiv - Microbiology 2021Quote: ... Virus was quantified using real-time PCR using SYBR Premix Ex Taq™ (Applied Biosystems, Thermo Fisher Scientific, USA) according to the manufacturer’s instructions ...