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Citations for Thermo Fisher :
451 - 500 of 10000+ citations for Recombinant Mouse Map2k1 His & GST tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Trichoplusia ni (Hi-5, Thermo Fisher Scientific) and Spodoptera fugiperda (Sf9 ...
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bioRxiv - Microbiology 2024Quote: ... 10% HI-FBS (v/v, Gibco 10082147) 100 U/mL penicillin ...
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bioRxiv - Cell Biology 2021Quote: ... Alexa-Fluor-488- or Alexa-Fluor-568-tagged donkey anti-mouse- or anti-rabbit-immunoglobulin antibodies (A32766, A32790, A10042, A10037, Molecular Probes, 1:200) and Alexa-Fluor-633 goat anti mouse (A-21050 ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissues were further incubated for 2 h at room temperature with HRP-tagged secondary antibody of goat anti-mouse (1:1000) (Life Technologies, Thermo Scientific, USA). The sections were then washed with TBST three times ...
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bioRxiv - Microbiology 2024Quote: ... Detection of the murine-human chimera 72A1 detection was performed using goat anti-mouse IgG (H+L) cross-adsorbed secondary antibody Alexa Fluor™ 488 tagged (Invitrogen #A-11001).
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bioRxiv - Bioengineering 2021Quote: ... recombinant human Vitronectin (Gibco A14700) was used at a final concentration of 25ug/ml ...
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bioRxiv - Cell Biology 2021Quote: ... Recombinant (Invitrogen, no. 10342-020). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Cancer Biology 2020Quote: Human recombinant IL-1β (ThermoFisher) was reconstituted in deionised water and administered at 10 ng/ml.
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bioRxiv - Microbiology 2022Quote: Purified recombinant β-lactamase (ThermoFisher) in PBS (0.15 nM ...
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bioRxiv - Developmental Biology 2019Quote: ... and recombinant human FGF2 (Invitrogen) at 20 ng/ml ...
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bioRxiv - Physiology 2021Quote: ... Recombinant CSF1R (Invitrogen, Carlsbad, CA) was used at a concentration of 2.5 nM ...
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bioRxiv - Cancer Biology 2024Quote: ... recombinant (Thermo Fisher Scientific, #10342020), and specific oligonucleotides (Supplementary Table S4 ...
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bioRxiv - Biochemistry 2019Quote: ... and CMG2-GST was affinity purified with Glutathione Superflow Agarose (Thermo Fisher). Glutathione was removed and CMG2 exchanged into HBS-T with 50% glycerol using Sephadex G50 (GE Life Sciences ...
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bioRxiv - Cell Biology 2020Quote: ... Cell lysates were incubated with 200μl of GST-Sepharose beads (Thermo Scientific) and washed 6 times with CHAPS buffer containing 0.5 M NaCl and 3 times with CHAPS buffer containing 5 mM MgCl2 ...
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bioRxiv - Cell Biology 2020Quote: ... 1 μl (0.21 μg) of PKR(252-551)-GST enzyme (Thermo Scientific) was added ...
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bioRxiv - Biochemistry 2021Quote: ... The filtered lysate was then added to GST-beads (Thermo Fisher Scientific) pre-equilibrated in binding buffer and incubated for 1 hour at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... GST proteins were captured on Pierce glutathione magnetic beads (Thermo Fisher Scientific) for 1 hour ...
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bioRxiv - Biophysics 2022Quote: ... except for passage of the final prep through a GST column (ThermoFisher). Briefly ...
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bioRxiv - Biophysics 2022Quote: ... Fractions containing GST-Cdc6 were treated with 200 U PreScission protease (ThermoFisher) for 2 h on ice ...
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bioRxiv - Molecular Biology 2019Quote: ... GST fusion proteins were expressed in Escherichia coli BL21 Star (DE3) (Invitrogen) or C41 (86 ...
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bioRxiv - Microbiology 2020Quote: ... The vector only encoding GST was transformed into BL21 DE3 (ThermoFisher Scientific). Plasmids encoding GST-YidC1CT or GST-YidC2CT were transformed into BL21 Star™ (ThermoFisher) ...
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bioRxiv - Cancer Biology 2023Quote: ... GST-ISG15 was expressed in Escherichia coli BL21(DE3) (Thermo Fisher, EC0114) at OD600 0.6-0.8 with 0.1 mM IPTG and 20°C and lysed in the lysis buffer by sonication ...
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bioRxiv - Cancer Biology 2022Quote: ... bacterially expressed GST-p21 bound to glutathione Sepharose beads (Thermo Scientific, 16100) were incubated with His-RanBPM ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Then GST was removed using 20 U/mg protein of Actev (Invitrogen) according to the instructions of the manufacturer.
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bioRxiv - Cell Biology 2024Quote: ... To detect PI(4,5)P2 a GST Tag Antibody (MA4-004; Invitrogen) was used ...
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bioRxiv - Immunology 2020Quote: ... were coated overnight at 4°C with 2 ug/ml of mouse anti-His-tag antibody (Invitrogen cat. #MA1-21315-1MG, Thermo Fisher Scientific) in PBS ...
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bioRxiv - Synthetic Biology 2023Quote: ... Membranes were incubated overnight (50 rpm, 4°C) with either mouse anti-His monoclonal (Thermo Fisher; 1:3000 in 5% skimmed milk) or anti-IFNa2 primary antibody (abcam ...
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bioRxiv - Bioengineering 2023Quote: ... Yeast cells were incubated with 1:1000 dilution of mouse anti-Myc antibody and 1:1000 dilution of chicken anti-His (Invitrogen, PA1-9531) antibodies on ice for 20 min ...
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bioRxiv - Bioengineering 2023Quote: ... the cells were washed once with ice-cold PBSB and incubated with 1000x dilution of mouse anti-Myc and chicken anti-His (Invitrogen, PA1-9531) antibodies on ice for 20 min ...
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bioRxiv - Molecular Biology 2021Quote: ... fluorescence-labeled HIV-1JR-FL Env(-) carrying the (His)6 epitope tag was incubated with biotin-conjugated anti-(His)6 tag antibody (HIS.H8, Invitrogen) at 4° for two hours.
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bioRxiv - Cell Biology 2019Quote: ... The plasmids encoding for His-SEPT2 or His-SEPT2-mCherry and SEPT6/7-strep were co-transformed into E.coli BL21 (DE3) (Invitrogen). Bacterial cultures were grown to OD600 of 2-3 and induced with 1 mM IPTG for 1h at 37°C (His-SEPT2 ...
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bioRxiv - Immunology 2021Quote: ... RBD-His and Spike-his containing supernatants were batch purified using the HisPur™ Ni-NTA Resin (ThermoFisher Scientific). Supernatants were incubated with 6 ml of resin for 1h at RT ...
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bioRxiv - Cell Biology 2020Quote: To compare the presence of the nucleolar rim of mNG-tagged proteins in live and fixed cells we fixed mNG-tagged cells in 4% formaldehyde (Thermo Scientific, 28908).
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bioRxiv - Cell Biology 2021Quote: ... AnkB440 KO primary cortical neurons were transfected with Halo-tagged AnkB440 or GFP-tagged AnkB220 plasmids at DIV0 by lipofection (Lipofectamine 2000, Thermo Fisher Scientific). After brain dissociation ...
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bioRxiv - Immunology 2022Quote: ... of the different constructs were ordered and cloned into a pPPI4 avidin-tagged and/or hexahistidine-tagged vector by PstI-BamHI digestion and ligation with Gibson Assembly (Thermo Fisher Scientific). The recombinant human ACE-2 receptor was obtained in the same way after ordering the corresponding gBlock gene fragment (Integrated DNA Technologies) ...
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bioRxiv - Molecular Biology 2023Quote: ... The mCherry tagged SETD6 WT or Y285A and EGFP-tagged E2F1 WT or K117R were co-transfected into cells using polyethyleneimine (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: NK cells were enriched and incubated with 50 ng/ml recombinant mouse IL-15 (Preprotech) for four days in RPMI plus 10 % FBS with penicillin and streptomycin (Thermofisher) added in a U-bottom 96-well plate at the concentration of two million cells per ml ...
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bioRxiv - Microbiology 2023Quote: ... and intracellular staining was performed using recombinant hu-mouse anti SeV-NP antibody conjugated with AlexaFluor-647 (1:1000) (Invitrogen) in combination with either anti-Krt5 (1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... 100 pmol (77 nM) of recombinant dynein tail complex or recombinant protein A (ThermoFisher Scientific) were incubated with 100 μL IgG magnetic beads in 1300 μL IgG binding buffer with rotation for 1 h at 4°C ...
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bioRxiv - Bioengineering 2022Quote: ... The secondary antibodies tagged with Alexa Fluor 647 (Invitrogen) at 5 μg/mL were added to the slices and incubated for 1 h before three-times washing and imaging.
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bioRxiv - Molecular Biology 2021Quote: ... or Alexa647-tagged antisera (1:500; Invitrogen, Karlsruhe, Germany) in TBS ...
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bioRxiv - Neuroscience 2020Quote: ... tagged with Alexa Fluor 594 or 488 dyes (Invitrogen), and the signal enzymatically augmented with sequential tyramide signal amplification (TSA amplification kits ...
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bioRxiv - Physiology 2020Quote: ... Amp-3 and HRP-tagged probe (Thermo Fisher Inc), one drop each for 30 ...
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bioRxiv - Immunology 2021Quote: ... and Rhodamine or Alexa Fluor 488-tagged Phalloidin (Invitrogen) were used for counterstaining cells ...
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bioRxiv - Plant Biology 2023Quote: ... antibodies and detected using fluorescently tagged secondary antibodies (Invitrogen, goat raised anti-mouse 488 and Anti-rabbit 555) ...
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bioRxiv - Molecular Biology 2023Quote: ... Whi5 tagged with GFP was obtained from Thermo Fisher Scientific GFP yeast clone collection ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissues were further incubated for 2 h at room temperature with HRP-tagged secondary antibody of goat anti-mouse (1:1000) (Life Technologies, Thermo Scientific, USA). The sections were then washed with TBST three times ...
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bioRxiv - Genomics 2022Quote: ... the HA-tagged protein of interest was detected with 1:500 diluted HA Tag Alexa Fluor 647 conjugated mouse antibody (ThermoFisher, Catalog no. 26183-A647). After antibody incubation ...
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bioRxiv - Immunology 2021Quote: Hela cells stably-expressing EGFP-tagged NLRP3 and mApple-tagged RAB5A were seeded into Nunc™ Lab-Tek™ 8-well Chambered Coverglass (155411, ThermoFisher Scientific) on the day before experiments ...
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bioRxiv - Biochemistry 2020Quote: CHO-K1 cells in 10-cm dishes were transfected with complementary DNA encoding FLAG-tagged mLPCAT2 or one of several FLAG-tagged mLPCAT2 mutants using Lipofectamine 2000 (Thermo Fisher Scientific, USA). At 48 h post-transfection ...