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Citations for Thermo Fisher :
451 - 500 of 10000+ citations for Recombinant Mouse Erbb2 protein Fc tagged APC labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2020Quote: ... Membranes were then incubated with anti-mouse Dylight800-labeled secondary antibody (Invitrogen) diluted 1:5000 in casein buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... 4 × 104 mouse T cells were labeled with CFSE (Life Technologies, C34554) and cultured for 3 days at 37C with 1ul of αCD3/28 bead (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... and Alexa Fluor 568 labeled donkey anti-mouse (1:1000, Life Technologies) in the dark for 1 hour ...
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bioRxiv - Cell Biology 2023Quote: ... were cultured in complete RPMI (10% FCS, 1% Penicillin/Streptavidin, 1% HEPES) supplemented with recombinant human M-CSF (10 ng/mL, Life Technologies) for 7-10 days to differentiate monocyte-derived macrophages (MDM) ...
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bioRxiv - Genomics 2024Quote: rox2RNA probes at 100 nM and hrp38 RNA probes at 50nM were incubated with MBP-tagged FL CLAMPWT protein or MBP-tagged FL CLAMPdelPrLD protein in REMSA binding buffer provided with the LightShift Chemiluminescent RNA EMSA kit (Thermo Scientific, USA) at room temperature for 30 min according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... into single-cell suspension and labeled using double staining with anti-TRA-1-81 APC (#17-8883-42, Thermo Fisher Scientific) and SSEA4 Alexa Fluor 488 (#14-8843-80 ...
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bioRxiv - Systems Biology 2023Quote: ... The following primary antibodies were used: 10 ul of allophycocyanin (APC)-labeled anti-CD2 antibody (clone LT2; Invitrogen, Catalog # MA1-10132), 5 uL of APC-labeled anti-CD81 antibody (clone 1D6 ...
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bioRxiv - Microbiology 2019Quote: ... cells were activated by adding 50 ng/mL recombinant mouse IFN gamma (Invitrogen). On day 7 ...
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bioRxiv - Microbiology 2022Quote: ... and stimulated with 10 ng/mL (~100 U/mL) recombinant mouse IFNγ (Gibco) for 24 h prior to infection ...
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bioRxiv - Immunology 2023Quote: ... Clec12a-Fc or Fc only fusion proteins were isolated with Pierce™ Classic Magnetic IP/Co-IP Kit (Thermo Fisher Scientific, Catalog number: 88804), quantified with Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific Catalog number ...
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bioRxiv - Immunology 2024Quote: ... the cells were probed with donkey anti-mouse secondary antibody tagged with Alexa Fluor 568 (Invitrogen), for 1 h in dark ...
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bioRxiv - Immunology 2021Quote: ... then incubacted 3 h with fluorescent-conjugated secondary antibodies diluted in saturation buffer (Goat anti-mouse IgG1-APC and Goat anti-mouse IgG2a A555 from Invitrogen). PCLS were washed 4 times with 300 μL of PBS (2x 5 min ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were then washed and stained with FITC-labeled goat-anti-rabbit-IgG and/or Texas Red-labeled goat-anti-mouse-IgG (Molecular probes) secondary antibodies ...
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bioRxiv - Pathology 2020Quote: ... The secondary antibodies used to reveal LC3 and LAMP2 were Alexa 594-labeled anti-rabbit secondary antibody and Alexa 546-labeled anti-mouse secondary antibody (Invitrogen, USA), respectively ...
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bioRxiv - Cancer Biology 2023Quote: ... they were incubated in secondary antibody conjugated to different fluorophores (Alexa 555-labeled anti-rabbit and Alexa 488-labeled anti-mouse secondary antibody (Thermo Fisher)) and DAPI for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... The cells were then washed with cold 1X PBS three times for 3 min each and incubated with Alexa 555-labeled anti-rabbit and Alexa 488-labeled anti-mouse secondary antibody (Thermo Fisher) at room temperature for 1 hour ...
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bioRxiv - Bioengineering 2023Quote: Rhodamine-tagged dextran (Invitrogen) was encapsulated into AlgLung and S-AlgLung hydrogels at a concentration of 0.5 mg/ml ...
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bioRxiv - Molecular Biology 2021Quote: ... Fab and Fc regions were separated using a Nab Protein A column (Thermo Scientific). Fabs were concentrated up to ~1 mg/mL using an Amicon Ultra 0.5 filter (10k cut-off ...
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bioRxiv - Synthetic Biology 2023Quote: ... and Fc fusions were purified with Protein A agarose resin (Thermo Fisher Scientific; 20334) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... Fc-PRL-13 was purified with Protein A agarose resin (Thermo Fisher Scientific; 20334) according to the manufacturer’s instructions with the exception that all buffers were supplemented with 1tab/50mL of cOmplete Protease Inhibitor Cocktail and 10uM Pepstatin A ...
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bioRxiv - Immunology 2023Quote: ... Purified proteins were labeled with Alexa Fluor 488 fluorescent dye using Microscale Protein Labeling Kits from Thermo Fisher, following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: Recombinant Bd37 (rBd37) protein was expressed as thioredoxin fusion protein from the pBAD/Thio-TOPO vector (Thermo Fisher Scientific), which encodes thioredoxin and histidine-tag at N-terminus and C-terminus of multi cloning site ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... These recombinant cells expressing individual rod opsin variants were then surface immunostained with a Dylight 550-labeled anti-HA antibody (ThermoFisher) and then fractionated according to the immunostaining intensity of expressed variants using FACS ...
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Cbln1 directs axon targeting by corticospinal neurons specifically toward thoraco-lumbar spinal cordbioRxiv - Neuroscience 2022Quote: CSN that project to lumbar spinal segments were retrogradely labeled at P5 with an Alexa Fluor 555-conjugated cholera toxin subunit B (CTB-555) recombinant retrograde tracer (Invitrogen). For these injections ...
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bioRxiv - Cancer Biology 2019Quote: ... washed and resuspended in FC buffer with goat-anti-mouse Alexa488 (Invitrogen; 1:400) for an additional 15 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... Detection was performed with secondary goat anti-mouse IgG Fc HRP antibody (Invitrogen, A16084) and WesternBright Sirius chemiluminescent HRP conjugate (advansta ...
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bioRxiv - Biochemistry 2021Quote: ... supplemented with 10% FCS (FCS, Thermo Fisher Scientific), 100 U ml−1 penicillin ...
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bioRxiv - Immunology 2023Quote: ... The cells were washed three times with flow staining buffer and labeled with a PE-conjugated anti-Human IgG Fc antibody (Thermo Fisher) for 30 mins ...
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bioRxiv - Immunology 2023Quote: ... or 5 µg/ml mouse IgG2a kappa Isotype control (eBM2a) APC (Thermo Fisher Scientific, USA) as the isotype control ...
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bioRxiv - Cell Biology 2023Quote: ... followed by staining with an APC-fluorescent anti-mouse secondary antibody (Molecular Probes, Eugene, OR) or with matched isotype controls for 60 min at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... Protein samples were labeled with TMT10plex isobaric mass tag labeling reagents (Thermo Scientific), at a concentration of 20 μg/μL in dry acetonitrile ...
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bioRxiv - Molecular Biology 2022Quote: Tau proteins were fluorescently labeled using amine-reactive DyLight488-NHS ester (Thermo Scientific) following manufacturer instructions ...
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bioRxiv - Microbiology 2021Quote: Purified reovirus was labeled using the Alexa Fluor 488 Protein Labeling Kit (Invitrogen). Briefly ...
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bioRxiv - Immunology 2020Quote: ... OVA was labeled with Alexa Fluor 488 Protein Labeling Kit (A10235, Thermo Fisher) and injected (5 μg ...
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bioRxiv - Cancer Biology 2022Quote: ... B16F10 cells were transfected with labeled sgRNAs and Cas9 protein (Thermo Fisher Scientific) using CRISPRMax Cas9 transfection reagent (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2022Quote: ... N protein was visualized with a secondary antibody labeled with AlexaFlour-488 (ThermoFisher). SARS-CoV-2 nucleocapsid (N ...
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bioRxiv - Cell Biology 2023Quote: ... and then transfected with mCherry-labeled RH proteins using lipofectamine 3000 (Thermo Fisher). Cells were allowed to recover for 48 H before being depolarized using CCCP for 1 H or starved via incubation in EBSS ...
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bioRxiv - Biophysics 2024Quote: ... the purified proteins were labeled with DyLight 488 dye (DL488, Thermo Fisher Scientific) or Alexa Fluor 647 dye (AF647 ...
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bioRxiv - Cancer Biology 2023Quote: ... the labeled proteins were reduced with 10-mM TCEP (Thermo Scientific, cat#77720) for 30 min at 50 ◦C and followed by alkylation with iodoacetamide (Thermo Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... To detect V5-tagged proteins a 1: 5,000 dilution of the primary anti-V5 antibody (Invitrogen, #R96025) and an anti-mouse horseradish peroxidase-conjugated secondary antibody (1:10,000 dilution ...
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bioRxiv - Neuroscience 2021Quote: ... Epitope-tagged proteins were eluted from beads by boiling for 5 min in LDS sample buffer (ThermoFisher). Anti-D-cysteine immunoprecipitates were eluted by incubating beads in 1 mM D-cys in TBS for 30 min at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... His-tagged proteins were purified using HisPur™ Ni-NTA resin (Thermo Fisher Scientific Cat. Nr. 88222) according to manufacturer’s instructions (batch protocol) ...
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bioRxiv - Microbiology 2020Quote: ... His tagged proteins were isolated from the supernatant using Dynabeads™ His-Tag Isolation and Pulldown (Invitrogen) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The His-tagged proteins were blotted using anti-6x His tag HRP-conjugated monoclonal antibody (Invitrogen, USA) and detected using Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Molecular Biology 2021Quote: ... Flag tagged proteins were immunoprecipitated from 2-3mg cellular extracts with Pierce Anti-DYKDDDDK Magnetic Agarose (Invitrogen). Beads were washed 5x with 1ml IP lysis buffer end eluted with 50µl 1xLaemmli buffer ...
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bioRxiv - Genetics 2021Quote: Plasmids expressing GST- and His-tagged proteins were transformed into BL21(DE3) competent cells (Thermo Scientific, EC0114) and grown in LB media at 37°C ...
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bioRxiv - Immunology 2022Quote: ... The His-tagged proteins were purified with the HisPur Ni-NTA Resin (Thermo Fisher Scientific cat.# 88221). After washing by wash buffer (25 mM Imidazole ...
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bioRxiv - Immunology 2023Quote: ... Filtered supernatants containing His-tagged proteins were passed slowly through HisPur Ni-NTA Resin (Thermo Fisher 88221) beads in columns ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein A-tagged strains were immunoprecipitated with Rabbit IgG-conjugated (MP-Biomedicals, SKU 085594) Dynabeads (Invitrogen, 14301) (Vakiloroayaei et al. ...
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bioRxiv - Biochemistry 2023Quote: ... FLAG-tagged ARL15 proteins were immunoprecipitated using 50 μL of Pierce Anti-DYKDDDDK Magnetic Agarose (ThermoFisher Scientific) for 2 hours at 4 ºC ...