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Citations for Thermo Fisher :
451 - 500 of 10000+ citations for Recombinant Human TNFRSF4 Protein Fc Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... GFP-tagged viral proteins were immunoprecipitated with anti-GFP antibody (G10362, Life Technologies) overnight followed by a 2-hour incubation with Protein G Dynabeads (Invitrogen) ...
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bioRxiv - Microbiology 2021Quote: ... Tagged proteins were visualized using the SuperSignal™ West Femto detection kit (ThermoFisher).
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bioRxiv - Systems Biology 2021Quote: ... The His-tagged proteins were purified on Ni-NTA resin (Thermo Scientific #88222) and washed into a buffer containing 50 mM Tris (pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The His-tagged protein was purified using HisPur Cobalt Resin (Thermo Fisher Scientific) in 20 mM Hepes (pH 7.3) ...
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bioRxiv - Cell Biology 2022Quote: His8-tagged NR2B proteins were labelled with Alexa 647 NHS ester (Thermo Fisher) and PrLD-SAMWT/PrLD-SAMME/PrLD proteins labelled with Alexa 555 maleimide (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2024Quote: ... HTP-tagged proteins were immunoprecipitated with IgG-coupled Dynabeads (tosylactivated M280, Thermo Scientific), RNAPII with antibody against Rpb1 (8WG16 ...
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bioRxiv - Biochemistry 2024Quote: ... AncΔ5 were tagged with Alexa Fluor 488 Microscale Protein Labeling Kit (Thermo Fisher), followed by the manufacturer’s protocol.
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bioRxiv - Biochemistry 2023Quote: ... The His-tagged protein was purified using HisPur Cobalt Resin (Thermo Fisher Scientific) in 20 mM Hepes (pH 7.3) ...
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bioRxiv - Plant Biology 2023Quote: ... The GST-tagged proteins were resuspended in phosphate-buffered saline (PBS) (Thermo Fisher) containing 1 mM dithiothreitol (DTT ...
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bioRxiv - Plant Biology 2023Quote: ... Myc-tagged proteins were detected using SuperSignal West Femto: SuperSignal substrates (ThermoFisher Scientific) in a 1:1 ratio ...
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bioRxiv - Cell Biology 2024Quote: ... His-tagged proteins were subsequently purified using Ni-NTA–agarose beads (Thermo Scientific) and elution buffer (20 mM HEPES ...
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bioRxiv - Neuroscience 2022Quote: ... were incubated with 100 ng/μL of mouse anti-human CD171 (L1CAM, neural adhesion protein) biotinylated antibody (clone 5G3, eBioscience/Thermo Fisher Scientific; Catalog # 13-1719-82); mouse anti-human GLAST (ACSA-1 ...
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bioRxiv - Biochemistry 2021Quote: ... ACE2-Fc was purified over Protein A Agarose (Thermo Fisher; 15918014). Once the Protein A Agarose was equilibrated in PBS buffer ...
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bioRxiv - Immunology 2023Quote: ... KIR-Fc were conjugated to protein-A Alexa 647 (Invitrogen, #P21462) overnight at 1:4 (molar ...
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Emergence of RNA-guided transcription factors via domestication of transposon-encoded TnpB nucleasesbioRxiv - Genetics 2023Quote: Chromatin immunoprecipitation of FLAG-tagged TnpB and TldR proteins was performed using Dynabeads Protein G (Thermo Fisher Scientific) slurry (hereafter ...
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bioRxiv - Systems Biology 2019Quote: ... Biotinylated probes were hybridized to GeneChip Human Gene 1.0 ST Arrays (Affymetrix, Santa Clara, CA) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2024Quote: ... Briefly hiPSCs were plated on low-attachment U-bottom 96 well plate and cultured in ESCM supplemented with ROCKi (50µM) and 4ng/ml Human FGF-basic Recombinant Protein (Gibco, Thermo Fisher Scientific, Cat no. PHG0024). At day 6 ...
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bioRxiv - Cancer Biology 2022Quote: ... we delivered recombinant Streptococcus pyogenes Cas9 protein (TrueCut™ Cas9 Protein v2; Thermo Fisher Scientific) together with the crRNA and tracrRNA into in vitro fertilized eggs of C57BL/6J mice according to the institutional procedure of Kyoto university (Ishibashi et al. ...
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bioRxiv - Developmental Biology 2024Quote: Recombinant FGF10 protein was labeled using an Alexa Fluor 488 Microscale Protein Labeling Kit (Invitrogen). To reduce the loss of physiological activity of FGF10 ...
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bioRxiv - Immunology 2024Quote: ... corresponding to the concentration of the highest standard of recombinant human IFNγ (Human IFNγ Gamma Uncoated ELISA kit, Invitrogen). IFNγ concentrations below the level of detection by the ELISA standard curve were set to 0 pg/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... recombinant His-tagged Endocan (R&D) was immobilized on 50 μl of HisPur Ni-NTA Magnetic Beads (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: Recombinant His-tagged Endocan (R&D) was immobilized on 50 μl of HisPur Ni-NTA Magnetic Beads (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... The protein with the C-terminal Fc-fusion was affinity purified with protein-A sepharose (Invitrogen). Samples were eluted with 0.1 M glycine pH 3.0 directly to a neutralizing buffer ...
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bioRxiv - Microbiology 2024Quote: ... Proteins fused with Fc were captured using Pierce Protein A/G Plus Agarose (Thermo Scientific, 20424), eluted with pH 3.0 glycine (100 mM in H2O) ...
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bioRxiv - Molecular Biology 2021Quote: ... and 50 ng/ml human recombinant M-CSF (ThermoFisher Scientific, Rockford, IL, USA). After six days of PBMC culture ...
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bioRxiv - Microbiology 2021Quote: ... 10 ng/ml of recombinant human fibroblast growth factor 2 (FGF2, Life Technologies), 20 ng/ml of recombinant human epidermal growth factor (EGF ...
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bioRxiv - Cell Biology 2021Quote: ... and recombinant human full-length bFGF (PHG0261, Gibco; Thermo Fisher Scientific, MA, USA) at 10 ng/ml.
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bioRxiv - Cell Biology 2021Quote: ... and recombinant human full-length bFGF (PHG0261, Gibco; Thermo Fisher Scientific, MA, USA) at 10 ng/ml.
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bioRxiv - Neuroscience 2022Quote: ... iPSCs were expanded on human recombinant vitronectin in StemFlexTM medium (Thermo Fisher Scientific). iBEC differentiation was performed as previously described by us [18] ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant human EGF and PDGF-BB were purchased from Gibco (Catalog No. PHG0311) and Peprotech (Catalog No ...
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bioRxiv - Microbiology 2022Quote: ... human recombinant epidermal growth factor (0.2 ng/mL, all from Gibco, Schwerte, Germany), isoproterenol (1 nM ...
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bioRxiv - Bioengineering 2022Quote: ... or 1.5 μg/cm2 of truncated recombinant human vitronectin (Vtn-N; ThermoFisher #A14700) for serum-free cells ...
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bioRxiv - Neuroscience 2022Quote: ... 10 µg/mL human recombinant insulin (ThermoFisher Scientific #12585014; stock = 4 mg/mL), 1:100 Penicillin-Streptomycin (ThermoFisher Scientific #15140122) ...
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bioRxiv - Immunology 2024Quote: ... at a 1:1 ratio and recombinant human IL-2 (Peprotech, ThermoFisher Scientific), and expanded in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Cell Biology 2023Quote: ... and recombinant human full-length bFGF (PHG0261, Gibco; Thermo Fisher Scientific, MA, USA) at 50 ng/ml24.
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bioRxiv - Cell Biology 2023Quote: ... and recombinant human full-length bFGF (PHG0261, Gibco; Thermo Fisher Scientific, MA, USA) at 50 ng/ml24.
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bioRxiv - Microbiology 2021Quote: ... which is composed of two ACE2 ectodomains linked to the Fc portion of the human IgG (Anand et al., 2020).Alexa Fluor-647-conjugated goat anti-human Abs (Invitrogen) were used as secondary antibodies to detect ACE2-Fc and plasma binding in flow cytometry experiments.
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bioRxiv - Molecular Biology 2021Quote: ... Biotinylated proteins were pulled down with Pierce High Capacity Streptavidin Agarose (20357; ThermoFisher) and were separated by SDS-PAGE and visualized using a SilverXpress silver stain kit (LC6100 ...
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bioRxiv - Neuroscience 2019Quote: ... Immunoprecipitation (IP) of transcripts was achieved via biotinylated-protein L (ThermoFisher Scientific 29997)-coated streptavidin magnetic beads (ThermoFisher Scientific 65602 ...
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bioRxiv - Cell Biology 2021Quote: ... Biotinylated proteins were purified using 260μl MyOne Streptavidin T1 beads (Novex Life Technologies) per 5mg total protein as described previously (22) ...
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bioRxiv - Microbiology 2020Quote: ... S1 proteins were pre-biotinylated using EZ-Link NHS-PEG4-Biotin (ThermoFisher Scientific). Following 20 minutes of pre-hydration of SA biosensors and 1 minute of sensor check ...
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bioRxiv - Immunology 2021Quote: ... Biotinylated protein was purified using a Zeba spin desalting column (Thermo Fisher Scientific). ACE2-Fc was then diluted to 20-83.3 mg/mL in PBS and loaded onto streptavidin biosensors (Forte Bio) ...
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bioRxiv - Immunology 2020Quote: ... was biotinylated using a Fluoreporter Mini-biotin XX protein labeling kit (Life Technologies) and detected using streptavidin-Qdot 585 ...
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bioRxiv - Immunology 2020Quote: ... Biotinylated-CoV proteins were captured on streptavidin coated 96-well plates (Thermo Scientific) by incubating with 100 µL of 2 µg/mL protein solution in TBS overnight at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... L-AHA labelled proteins were biotinylated using PEG4 carboxamide-Propargyl Biotin (ThermoFisher, B10185) and biotin-labelled proteins were isolated by immunoprecipitation using streptavidin magnetic beads (NEB ...
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bioRxiv - Cell Biology 2022Quote: ... Biotinylated proteins were captured using Pierce Streptavidin Magnetic Beads (Thermo Fisher Scientific, 88817) at 4°C on a rotator for 3 h ...
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bioRxiv - Cancer Biology 2024Quote: ... Biotinylated proteins were detected with NeutrAvidin DyLight 800 (Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Cell Biology 2023Quote: ... Biotinylated proteins were detected with streptavidin coupled to Alexa Fluor 594 (Thermo Fisher).
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bioRxiv - Immunology 2023Quote: ... The biotinylated proteins were tetramerized by adding APC-conjugated streptavidin (Thermo Fisher Scientific) at an allergen ...
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bioRxiv - Molecular Biology 2023Quote: ... Biotinylated proteins were enriched using magnetic streptavidin beads (Dynabeads M-280, Invitrogen, USA). The beads were washed with a series of SDS buffer ...