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Citations for Thermo Fisher :
451 - 500 of 8732 citations for IL 17RA Human HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... and mouse anti-human α-synuclein 1:1000 (Thermo Scientific; Rockford, IL, USA) or goat anti-luciferase 1:250 (Novus Biological ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The ELISA was performed using Human IL-1β high sensitivity kit from Invitrogen. The sample of ELISA used in the present study consist of cell culture media (referred to as sample hereafter ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The ELISA was performed using Human IL-1b high sensitivity kit from Invitrogen. The samples for ELISA were media collected after treating the cells with drugs at 100uM ...
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bioRxiv - Immunology 2024Quote: ... at a 1:1 ratio and recombinant human IL-2 (Peprotech, ThermoFisher Scientific), and expanded in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Molecular Biology 2019Quote: ... The knottin Fc fusion proteins were expressed in human embryonic kidney (HEK293) cells following the manufacturer’s protocols in the FreeStyle MAX 293 Expression System (Invitrogen). Secreted knottin-Fc fusion proteins were purified using Protein A Sepharose (Sigma ...
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bioRxiv - Bioengineering 2023Quote: ... Protein expression was achieved by transient transfection of FreeStyle human embryonic kidney 293 (HEK293-F) cells (Thermo Fisher Scientific). Cells were grown in non-baffled shake flasks (Corning ...
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A quantitative analysis of the interplay of environment, neighborhood and cell state in 3D spheroidsbioRxiv - Systems Biology 2020Quote: T-REx-293 cells (Invitrogen) and DLD1 cells (Flp-In T-Rex DLD-1 ...
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bioRxiv - Microbiology 2021Quote: ... T-REx-293 cells (Invitrogen) were grown in DMEM supplemented with blasticidin (10 µg/mL ...
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bioRxiv - Microbiology 2021Quote: T-REx-293 cells (Invitrogen), which constitutively expresses the Tet repressor (TetR ...
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bioRxiv - Microbiology 2021Quote: FreeStyle 293-F (Thermo Fisher) cells were grown to a density of 1×106 cells/mL at 37°C with 8% CO2 with regular 135 rpm agitation ...
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bioRxiv - Systems Biology 2022Quote: ... ExpiFectamine 293 Reagent (Thermo Fisher) was diluted with Opti-MEM separately then combined with diluted plasmid DNA for 10 minutes at room temperature ...
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bioRxiv - Synthetic Biology 2020Quote: ... Flp-In 293 cells (Invitrogen) were cultured in DMEM media supplemented with fetal bovine serum (10% ...
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bioRxiv - Cancer Biology 2020Quote: ... GSE6532 ((n= 293, platform: Affymetrix Human Genome U133A and B Array ...
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bioRxiv - Molecular Biology 2022Quote: T-Rex-293 cells (Invitrogen) were cultured and maintained as previously described63 in DMEM (Gibco 11-965-092 ...
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bioRxiv - Physiology 2022Quote: ... ExpiFectamine 293 Reagent (Thermo Fisher) was diluted with Opti-MEM separately then combined with diluted plasmid DNA for 10 minutes at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... FreeStyle 293-F (Thermo Fisher) cells were transiently co-transfected using PEI MAX (Polysciences ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Colorimetric protein assays were conducted using commercial human IL-8 ELISA and mouse IL-6 ELISA kits (Invitrogen; 88-8086, 88-7064) according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2021Quote: ... Supernatant was harvested at the indicated timepoints and IL-2 levels in the supernatant were measured via IL-2 Human Instant ELISA kit (Thermo Fisher #BMS221INST). T-cell proliferation was also measured at the indicated timepoints using a BD FACSymphony Fortessa X-50.
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bioRxiv - Biochemistry 2023Quote: ... Detection of bound Af2-IL was performed using a rat anti-human IL-2 mAb Alexa Fluor488 conjugate (Invitrogen, Carlsbad CA, USA). Cell nuclei were counterstained with DAPI (Sigma Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... The level of IL-8 in cell culture supernatants was determined using a Human IL-8 Uncoated ELISA kit (Invitrogen, ThermoFisher Scientific Inc.).
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bioRxiv - Microbiology 2020Quote: ... ATCC CRL-1586) and HEK-293T (human embryonic kidney cells) cells were maintained in Dulbecco’s Modified Eagle Medium (Gibco). All media were supplemented with 10% fetal bovine serum (Hyclone) ...
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bioRxiv - Immunology 2020Quote: ... human embryonic kidney cells (HEK) 293T were grown in a T75 flask with Dulbecco’s modified eagle medium (DMEM) (Gibco) + 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2022Quote: ... human embryonic kidney 293T cells (HEK; CRL-3216; ATCC; RRID: CVCL_0063) were cultured in DMEM media (12430062; Gibco™) with 10% FBS (098-150 ...
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bioRxiv - Microbiology 2022Quote: Human HEK-293T adherent cells were seeded at 37°C with 5% CO2 in DMEM + GlutaMAX™ (Gibco, Germany) containing 10% heat inactivated fetal calf serum (dFCS ...
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bioRxiv - Microbiology 2023Quote: Human telomerase immortalized fibroblasts (MRC5-hT) and HEK 293T cells were cultured in Dulbecco’s Modified Eagle Media (DMEM; Gibco) supplemented with 10% (vol/vol ...
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bioRxiv - Neuroscience 2023Quote: ... and Gentamycin 40µg/mL and Human embryonic kidney (HEK) 293T cells were cultured in DMEM (1g/L glucose) (GIBCO) supplemented with 10% Fetal Calf Serum (FCS) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cDNA plasmids in pcDNA™4/TO Mammalian Expression Vector were transiently transfected into Expi293F™ cells stably expressing human FHF2b and human SCN1B subunit (polyclonal) background using ExpiFectamine™ 293 Transfection Kits (Gibco,Thermo Fisher Scientific CAT #: A14524). Induction was achieved using Tetracycline (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2019Quote: ... or an untransfected wild type HEK Flp-in cell line (HEK wt, Invitrogen, R75007) were cultured in high-glucose DMEM (HyClone ...
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bioRxiv - Cell Biology 2020Quote: ... HEK 293Trex ERGIC53 KO or HEK 293Trex SURF4 KO cells using lipofectamine 2000 (Invitrogen) or polyethyleneimine (PEI) ...
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bioRxiv - Immunology 2022Quote: ... HEK 293F cells were maintained in HEK 293F in 293FreeStyle expression medium (Life Technologies) and cultured at 37°C with 8% CO2 while shaking at 125 rpm ...
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bioRxiv - Neuroscience 2020Quote: FreeStyle 293-F cells grown in serum-free FreeStyle 293 Expression Medium (Gibco, 12338-018) were transfected with Sez6L2MH or His-DAF plasmids using Fectopro (Polyplus ...
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bioRxiv - Microbiology 2019Quote: ... 293 F cells were thawed and incubated with FreeStyleTM 293 Expression Medium (Life Technologies, CA) in a shaker incubator at 37°C ...
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bioRxiv - Synthetic Biology 2021Quote: ... HEK-293FT cells (Thermo Fisher), and HeLa cells (ATCC ...
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bioRxiv - Synthetic Biology 2020Quote: ... HEK-293FT cells (Thermo Fisher), HeLa cells (ATCC) ...
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bioRxiv - Microbiology 2021Quote: ... HEK-293T cells (Life Technologies) were maintained in Dulbecco’s modified Eagles medium (DMEM ...
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bioRxiv - Biochemistry 2022Quote: HEK Viral Production cells (ThermoFisher) were maintained in suspension in Freestyle F17 (ThermoFisher ...
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bioRxiv - Physiology 2023Quote: HeLa and HEK 293T (Invitrogen) cells were grown in high glucose Dulbecco’s Modified Eagle Medium with high glucose ...
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Immunogenic fusion proteins induce neutralizing SARS-CoV-2 antibodies in the serum and milk of sheepbioRxiv - Bioengineering 2022Quote: ... Expi293 HEK cells (Thermofisher, #A14527) were expanded and passaged in Expi293 expression media (Thermofisher ...
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bioRxiv - Neuroscience 2023Quote: ... HEK-293T cells (Life Technologies) were cultured in DMEM High Glucose (4.5 g/L ...
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bioRxiv - Biophysics 2023Quote: ... and HEK 293A (Thermo Fisher) cells were cultured at 37 °C in a 5% CO2(g ...
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bioRxiv - Cancer Biology 2024Quote: The HEK 293FT (Invitrogen, # R70007) cell line was transfected with either pBabe-puro-HA-KRASG12C (Addgene ...
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bioRxiv - Molecular Biology 2024Quote: HEK Viral Production cells (ThermoFisher) were maintained in suspension in Freestyle F17 (ThermoFisher ...
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bioRxiv - Biochemistry 2021Quote: The knock-out cell line (GTPBP5KO) was generated in the Flp-In T-Rex human embryonic kidney 293 (HEK293T) cell line (Invitrogen) using CRISPR/Cas9 technology targeted on exon 1 of MTG2 gene ...
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bioRxiv - Biochemistry 2021Quote: Human embryonic kidney 393T cells were adapted to suspension culture in Gibco Freestyle 293 medium (Thermo Fisher Scientific, Waltham USA) supplemented with 20 U/L penicillin ...
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bioRxiv - Biochemistry 2021Quote: Human embryonic kidney cells adapted to suspension culture (HEK293F) were grown in Free Style 293 Expression Medium (Thermo Fisher Scientific) and harvested at a density of 3 million cells/ml ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The ELISA was performed using Human IL-1ẞ high sensitivity kit sold by Invitrogen. The sample for our ELISA consisted of cell culture media (referred to as sample hereafter ...
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bioRxiv - Immunology 2022Quote: ... Human and mouse IL-1β secretion was quantified by ELISA kits (Thermo Fisher Scientific) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: ... ELISA was performed with Human IL-8 ELISA Ready-SET-Go kit (Affymetrix eBioscience) according to manufacturer’s protocol.
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bioRxiv - Cell Biology 2022Quote: ... and IL-17A Human Uncoated ELISA Kit (all Thermo Fisher Scientific, Waltham, MA, USA), respectively ...
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bioRxiv - Microbiology 2023Quote: ... The level of IL-8 in cell culture supernatants was determined using a Human IL-8 Uncoated ELISA kit (Invitrogen, ThermoFisher Scientific Inc.).