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Citations for Thermo Fisher :
451 - 500 of 10000+ citations for EGF Mouse His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... were conjugated at a molar ratio of 1:1 with biotinylated EGF (ThermoFisher) at a concentration of 0.66 nM ...
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bioRxiv - Cancer Biology 2020Quote: ... 250μl of small intestinal organoid growth media (Basal Media containing 50 ng/mL EGF (Invitrogen PMG8043), 100ng/ml Noggin (Peprotech 250-38) ...
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bioRxiv - Cancer Biology 2020Quote: ... EGF and FGF growth factors (10ng/ml each, final;ThermoFisher #PHG0266 and #PHG0315), and Gentamicin (1:1,000;Fisher #BW17 518Z) ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant human EGF and PDGF-BB were purchased from Gibco (Catalog No. PHG0311) and Peprotech (Catalog No ...
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bioRxiv - Developmental Biology 2022Quote: ... Complete growth medium was prepared by adding murine EGF (50ng/ml) (Gibco, TX), R-spondin-1 (conditioned medium 1ml/50ml ...
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bioRxiv - Cancer Biology 2023Quote: Measurement of EGFR internalization was performed by using pHrodo Red EGF (Invitrogen P35374) at a concentration of 0.5 ug/ml ...
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bioRxiv - Cell Biology 2024Quote: ... incubated with 100 ng/ml EGF-Alexa 555 (Thermo Fisher Scientific, MA, USA) for 5 min ...
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bioRxiv - Biochemistry 2019Quote: ... SAMS peptide substrate (His-Met-Arg-Ser-Ala-Met-Ser-Gly-Leu-His-Leu-Val-Lys-Arg-Arg, obtained from Invitrogen, USA) was used for the assay ...
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bioRxiv - Biochemistry 2020Quote: ... the magnetic beads were resuspended in 10.0625 μL ROX/Hi-Di (0.0625 μL of ROX 350 ladder, Applied Biosystems, in 10 μL of Hi-Di formamide, Applied Biosystems) and incubated at room temperature for 20 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... the magnetic beads were resuspended in 10.0625 μL ROX/Hi-Di (0.0625 μL of ROX 350 ladder [Applied Biosystems] in 10 μL of Hi-Di formamide [Applied Biosystems]) and incubated at room temperature for 20 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... Cry1 pcDNA4A-myc-his and Cry2 pcDNA4A-myc-his plasmids were double digested with NotI and XhoI FastDigest enzymes (Thermo Scientific) and used as host plasmids ...
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bioRxiv - Biochemistry 2023Quote: Plasmids of pET28a-his TEV-glnR or pET28a-his TEV-glnR derivative was firstly transformed into expression strain BL21(DE3) (Invitrogen, Inc.). then single colonies of the positive transformants were inoculated and amplified with 5 L LB broth supplemented with 50 μg/ml kanamycin at 37 °C with shaking ...
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bioRxiv - Microbiology 2019Quote: ... His-FruB or His-HPr in the cell extract was purified using HisPurTM-Ni-NTA Superflow Agarose (from Thermo Scientific, Cat # 25214) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: Recombinant SARS-CoV-2 S-RBD fused with Fc/His tag (S-RBD-His) was produced in 293T cells and purified with Protein A Agarose (Thermo Fisher Scientific). Recombinant human ACE2-ECD fused with Flag/His tag was produced in 293T cells and purified with anti-DYKDDDDK G1 Affinity Resin according to the manufacturer’s instructions (GenScript).
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bioRxiv - Microbiology 2019Quote: ... and 5 μg.L−1 of epidermal growth factor (EGF) human recombinant (Thermo Fisher Scientific) (Wu et al ...
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bioRxiv - Microbiology 2020Quote: ... EGF (20 ng/mL) and StemPro Neural Supplement (2% [vol/vol]) (Thermo Fisher Scientific). NSCs were differentiated into HMNs utilizing a neuronal differentiation medium prepared in Neurobasal Medium with B-27 Serum-Free supplement (2% [vol/vol] ...
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bioRxiv - Systems Biology 2020Quote: ... 500 μl crypt culture medium containing growth factors EGF (50 ng/ml, Life Technologies), Noggin (100 ng/ml ...
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated with A647-complexed EGF (1:300; Molecular Probes, Cat# E-35351) for 15 min ...
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bioRxiv - Genomics 2022Quote: ... and grown in basal media containing 50 ng/μL EGF (Invitrogen Waltham, MA; PMG8043), 100 ng/μL Noggin (PeproTech ...
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bioRxiv - Neuroscience 2022Quote: ... complexed to Alexa Fluor 488 streptavidin (Alexa FluorTM 488 EGF complex) (1:100, Invitrogen), APC-Cy7 rat anti-mCD45 (1:200 ...
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bioRxiv - Cell Biology 2022Quote: ... 4h prior treatment with recombinant human epidermal growth factor (EGF) (Cat# RP-10927, Invitrogen) at 5 ...
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bioRxiv - Neuroscience 2023Quote: ... with murine natural EGF (10 or 20 ng/ml, Thermofisher Scientific, Waltham, MA, USA) and human recombinant FGF-2 (10 ng/ml ...
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bioRxiv - Neuroscience 2023Quote: ... the medium was also supplemented with EGF and α (both 10 ng/ml; Gibco) every 3-4 days and primary neurospheres were counted and imaged on day 5 and 10 of the culture to measure their diameter ...
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bioRxiv - Neuroscience 2023Quote: ... and Alexa Fluor 647-conjugated recombinant human EGF (Invitrogen, #35351, Lot# 1700382, 1:1000) to label EGFR.
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bioRxiv - Immunology 2023Quote: ... supplemented with 294.4 ng/l human recombinant Epidermal Growth Factor (EGF) (Gibco, 10450-013), 25 mg/l Bovine Pituitary Extract (Gibco ...
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bioRxiv - Genetics 2020Quote: ... pAc5.1/V5-His version C (V411020, Thermo Fisher Scientific). In order to test the transferring ability of gypsy ...
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bioRxiv - Developmental Biology 2021Quote: ... and inserted into pMT/V5-His A (Thermo Scientific) using XhoI and SpeI target sites ...
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bioRxiv - Cell Biology 2022Quote: pAc5.1/V5-His vector (Thermo Fisher Scientific, Cat #V411020) was used to construct plasmids for expressing proteins or miRNAs in Drosophila S2 cells ...
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bioRxiv - Molecular Biology 2019Quote: ... and Ion PGM Hi-Q Sequencing Kit (Life Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... with 10% heat-inactivated FBS (HI-FBS; Life Technologies)) ...
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bioRxiv - Biochemistry 2020Quote: ... the pBAD/His B vector (Invitrogen-Thermo Fisher, USA) was linearized by PCR with appropriate primers that contained an introduced ribosomal binding site and SalI and HindIII restriction sites to generate pBAD30_SalRBS ...
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bioRxiv - Biochemistry 2020Quote: ... the pBAD/His B vector (Invitrogen-Thermo Fisher, USA) was linearized by PCR with appropriate primers that contained an introduced ribosomal binding site and SalI and HindIII restriction sites to generate pBAD30_SalRBS ...
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bioRxiv - Neuroscience 2020Quote: ... heat-inactivated 10% fetal bovine serum (FBS HI, Gibco), 100 U/ml penicillin and 100 μg/ml streptomycin (Gibco) ...
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bioRxiv - Microbiology 2020Quote: ... and 9.9 μl of Hi-Di formamide (Applied Biosystems). These were then incubated for 5 min at 95°C and placed immediately on ice ...
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bioRxiv - Microbiology 2021Quote: ... Indicated amounts of recombinant GFP-His (Thermo Fisher # A42613) or SARS-CoV-2 N-His (Sino Biological # 40588-V08B ...
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bioRxiv - Immunology 2020Quote: ... and ligated into pcDNA3.1D/V5-His-TOPO vector (Invitrogen). Pseudoviruses were prepared by co-transfecting 1.25 µg of HIV-1 envelope containing plasmid with 2.5 µg of an envelope deficient HIV-1 backbone (PSG3Δenv ...
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bioRxiv - Molecular Biology 2020Quote: ... resuspended in Hi-Di formamide (Applied Biosystems/Life Technologies) and run on an Applied Biosystems 3730xl Genetic Analyzer ...
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bioRxiv - Molecular Biology 2020Quote: ... resuspended in Hi-Di formamide (Applied Biosystems/Life Technologies) and run on an Applied Biosystems 3730xl Genetic Analyzer ...
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bioRxiv - Molecular Biology 2022Quote: ... Pre-washed 25 µl His-Tag Dynabeads (ThermoFisher Scientific) were added ...
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bioRxiv - Neuroscience 2019Quote: ... heat-inactivated 10% fetal bovine serum (FBS HI, Gibco), 100 U/ml penicillin and 100 μg/ml streptomycin (Gibco) ...
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bioRxiv - Immunology 2020Quote: ... Primary antibodies against the His-tag (1:2000, Invitrogen) were followed by secondary HRP conjugated antibody (1:2000 ...
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bioRxiv - Microbiology 2021Quote: ... Primary antibodies against the His-tag (1:2000, Invitrogen) were followed by secondary HRP conjugated antibodies (1:2000 ...
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bioRxiv - Biophysics 2019Quote: ... 1 nM LanthaScreen Elite Tb-anti-His Antibody (ThermoFisher), and 400 TRAP220 peptide in a buffer containing 20 mM potassium phosphate (pH 7.4) ...
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bioRxiv - Molecular Biology 2019Quote: ... All proteins were expressed in Hi-Five cells (Invitrogen) from Trichoplusia ni using Baculovirus and the cultures were harvested 60 hours after infection ...
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bioRxiv - Biochemistry 2021Quote: ... Hi-5 cells (BTI-TN-5B1-4) (Gibco #B85502) were cultured in Express Five™ SFM (Serum-Free Media ...
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bioRxiv - Immunology 2021Quote: 100 µL of Invitrogen His-Tag Dynabeads (ThermoFisher 10104D) were used for prefusion S2 depletion ...
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bioRxiv - Developmental Biology 2020Quote: ... and cloned into the pAc5.1-V5/His vector (Invitrogen). Immunoprecipitations between Bdbt-EGFP and Myc-DAnkrd49 used GFP rabbit serum (ab290 ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were cloned into pcDNA-V5/His (Invitrogen) using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) ...
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bioRxiv - Molecular Biology 2021Quote: ... The pcDNA4-TO-myc/his B backbone vector (Invitrogen) was chosen for overexpression ...
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bioRxiv - Molecular Biology 2022Quote: ... 15 mL 6x His-Tag Monoclonal antibody (MA121315; Invitrogen) diluted 1:1500 in blocking solution supplemented with 0.1% NaN3 was added to the membrane and incubated at 4°C on an orbital shaker at 55 rpm for 14-16 h ...