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Citations for Thermo Fisher :
451 - 500 of 10000+ citations for Carboxypeptidase Y from Baker's Yeast since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... to produce tandem mass spectra whose b and y ion series patterns were compared to Sequest (Thermo Fisher Scientific) to establish protein identity ...
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bioRxiv - Biochemistry 2022Quote: ... High-resolution data sets for y-mtRNAP IC2 and y-mtRNAP IC4 to IC6 were collected on the Glacios using EPU software version 2.9.0 (ThermoFisher Scientific).
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bioRxiv - Bioengineering 2023Quote: ... plates at a concentration of 1.5×106 cells/mL in CB media supplemented with 10 µM Y-27632 and 1× Pen/Strep (Gibco). The plates were incubated at 37 °C and 5% CO2 for 72 hours ...
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bioRxiv - Developmental Biology 2024Quote: ... plus DNase and 10 μM Y-27632) for 1 hour with live/dead staining dye (SYTOX, Thermo Fisher Scientific) to exclude dead cells ...
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bioRxiv - Developmental Biology 2022Quote: Yeast two-hybrid (Y2H) interactions were characterized by employing the ProQuest Two-Hybrid System (Invitrogen Life Technologies) as reported in Cruz-Ramirez et al. ...
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bioRxiv - Developmental Biology 2022Quote: Yeast two-hybrid (Y2H) interactions were characterized by employing the ProQuest Two-Hybrid System (Invitrogen Life Technologies) as reported in Cruz-Ramirez et al. ...
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bioRxiv - Plant Biology 2020Quote: ... At1g08710 was further subcloned in yeast two hybrid vector pDEST-GBKT7 using Gateway cloning technology (Invitrogen, CA). Cloning was confirmed by colony PCR and sequencing.
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bioRxiv - Plant Biology 2021Quote: ... Plasmids used for yeast two-hybrid or Co-IP were generated by gateway LR cloning (Life Technologies) using the ENTRY vectors described above and appropriate destination vectors listed in Suppl ...
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bioRxiv - Systems Biology 2020Quote: ... The hybridisation was performed in 50μL of Stellaris hybridisation buffer complemented with 250μg.mL-1 Yeast tRNA (Ambion) and 5mM Ribonucleoside Vanadyl Complex (New England Biolabs) ...
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bioRxiv - Microbiology 2020Quote: ... The sequence-verified open reading frames were subcloned into a pYES-DEST52 yeast expression vector (ThermoFisher Scientific) by the LR reaction ...
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bioRxiv - Cell Biology 2021Quote: ... or Quasar 570 probes targeting mad1 were mixed with 2 μL each of yeast tRNA (Life Technologies) and Salmon sperm DNA (Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... Mating-type switch was confirmed by PCR on ultrapure gDNA (Yeast DNA Extraction Kit 78870, ThermoFisher Scientific) with oligos PR_26_D7+PR_26_D8 ...
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bioRxiv - Neuroscience 2020Quote: ... The acetylated sections were washed and incubated in hybridization buffer (50% formamide, 0.25 mg/mL yeast RNA, 0.5 mg/mL herring sperm DNA, 5x Denhard’s, 5x SSC, Invitrogen) at room temperature for 1 hr ...
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bioRxiv - Immunology 2020Quote: The H99S strain [21] of Cn serotype A was grown in yeast peptone dextrose (YPD; ThermoFisher Scientific) broth ...
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bioRxiv - Molecular Biology 2021Quote: ... 150 ng of total RNA was incubated with 75 U of yeast poly(A) polymerase (PAP) (Affymetrix) in the presence of GTP/ITP mix at 37°C for 60 min ...
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bioRxiv - Immunology 2021Quote: ... Yeast codon-optimised sequences of the Tp1 and Tp2 gene were synthesized by GeneArt (Thermo Fisher Scientific) and codon-optimised sequences for expression of Tp2 and Tp9 gene in eukaryotic cells were kindly provided by Dr ...
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bioRxiv - Microbiology 2021Quote: ... Depletion of rRNA was accomplished using the Ribominus Yeast and Bacteria transcriptome isolation kit (Invitrogen, Waltham, MA) and included ethanol precipitation with glycogen to concentrate remaining mRNA ...
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bioRxiv - Molecular Biology 2023Quote: ... Yeast cells were then harvested for RNA extraction using a RiboPure RNA Purification Kit (Invitrogen, cat#AM1926), which involves mechanical cell wall disruption ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 µL 32P-phosphorylated target RNA (∼1000 cpm/µL) and 1 µg yeast tRNA (Thermo Fisher Scientific) was added to each reaction ...
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bioRxiv - Biophysics 2023Quote: ... live yeast cells were immobilized in 8-well microscope chamber slides (Nunc Lab-tek, Thermo Fisher Scientific) pre-coated with 1-2 mg/mL concanavalin-A (MP Biomedicals) ...
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bioRxiv - Biophysics 2023Quote: ... live yeast cells were immobilized in 8-well microscope chamber slides (Nunc Lab-tek, Thermo Fisher Scientific) pre-coated with 1-2 mg/mL concanavalin-A (MP Biomedicals) ...
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bioRxiv - Plant Biology 2023Quote: ... Yeast colony polymerase chain reaction (PCR) was done using the Phire polymerase following manufacturer’s instructions (Thermo Fisher).
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bioRxiv - Plant Biology 2022Quote: ... and subsequently cloned into pGILDA and pB42AD yeast-two-hybrid vectors (Clonetech) via LR Gateway Reactions (ThermoFisher). To introduce T653L and Y643* mutations into ARFd1 entry clone ...
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bioRxiv - Molecular Biology 2024Quote: ... 150 ng of total RNA was incubated with 75 U of yeast poly(A) polymerase (PAP) (Affymetrix) in the presence of GTP/ITP mixure at 37°C for 60 min ...
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bioRxiv - Synthetic Biology 2023Quote: ... and a rabbit polyclonal anti-eEF2 (yeast) antibody at a 1:25,000 dilution (sample loading control) (ThermoFisher), followed by a secondary anti-rabbit antibody at a 1:20,000 dilution (Sigma Aldrich).
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bioRxiv - Bioengineering 2024Quote: ... and 1.0 L tap water (Kgs. Lyngby, Denmark)) or ISP2 (yeast extract 4g/L (Thermo Fisher Scientific), malt extract 10g/L (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2022Quote: ... hybridization buffer had additional 50 µg/ml yeast tRNA and 1x Denhardt’s solution (Invitrogen, Waltham, Massachusetts. U.S.), all post-hybridization washes were carried out using 5x SSC ...
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bioRxiv - Molecular Biology 2024Quote: ... and the aqueous phase was mixed with 100 μL of 200 μg/μL total yeast RNA (Ambion).
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bioRxiv - Microbiology 2024Quote: ... followed by the addition of 1 μl of yeast RNA (1 mg/ml, #AM7118, Thermo Fisher Scientific), and 1 μl of 10× structure buffer (0.1 M Tris– HCl ...
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bioRxiv - Physiology 2021Quote: ... Crosslinking of Y-3 antibody and Protein A+G was done using sulfosuccinimidyl (4-iodoacetyl)aminobenzoate (Sulfo-SIAB, ThermoFisher 22327). For 1 mL of protein A+G agarose (ThermoFisher/Pierce #20421) ...
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bioRxiv - Microbiology 2021Quote: ... and were maintained and virally transduced in F-media with mitomycin C treated 3T3 cells and Rho Kinase inhibitor (Y-27632, ThermoFisher) as previously described (40).
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bioRxiv - Microbiology 2021Quote: ... Raji B lymphocyte and Raji DC-SIGN cell lines (kindly provided by Y. Van Kooyke) were maintained in Roswell Park Memorial Institute medium (RPMI; Invitrogen) or RPMI plus 1 mg/mL geneticin (Invitrogen) ...
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bioRxiv - Cell Biology 2022Quote: ... Epithelial cell culture medium containing Y-27632 consisted of DMEM/F12 in a 3:1 ratio containing 1x penicillin–streptomycin (Gibco), 5% fetal bovine serum (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... Y-A or YIY-AAA) and FLAG-tagged CDK-1 constructs were expressed in FreeStyle 293-F cells (ThermoFisher Scientific). Cells were transfected using FreeStyle MAX Reagent and OptiPRO SFM according to the manufacturer’s guidelines (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... subcloned into pENTR3C-HA-Bcl-xL, and then recombined into RCASBP-Y-DEST (Loftus et al, 2001) using LR Clonase (Invitrogen) according to the manufacture’s instruction ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 20 µM Rho-associated coiled-coil forming protein serine/threonine kinase inhibitor (ROCK-I, compound Y-27632) (ThermoFisher) and transferred to a 6-well ultra-low attachment plate (Corning ...
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bioRxiv - Biophysics 2024Quote: PICASSO30 was used for lateral single molecule localisation (X-Y) and lateral drift correction was applied using PICASSO via fiduciary markers (0.1 µM T7279 TetraSpeck Microspheres, Life Technologies). Pixel intensities were rescaled to a range of 0 to 1 by dividing by the maximum pixel intensity (65535 ...
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bioRxiv - Physiology 2024Quote: ... at 37°C and continuous stirring in a cuvette with 300 μL Buffer Y containing 1 μL Calcium Green-5N (Invitrogen) as a fluorescent probe specific to Ca2+ ...
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bioRxiv - Neuroscience 2024Quote: ... Correct image reconstruction and alignment for dual labeled images were assured using the X-Y coordinates of 3-6 100 nm fiducial beads (T7279, Thermofisher) as reference points to align signals with an affine/linear registration function using Localization Microscopy Analyzer (LAMA ...
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bioRxiv - Cell Biology 2020Quote: ... The chemiluminescent signal in yeast samples was generated by the SuperSignal West Pico Plus chemiluminescent substrate (Thermo Scientific) and detected on a G-box transilluminator (Syngene).
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bioRxiv - Bioengineering 2020Quote: ... All sequences were cloned into the entry vector pDRf1-GW for yeast expression (28) through Gateway cloning (Invitrogen).
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bioRxiv - Plant Biology 2021Quote: ... and cloned into the yeast expression vector pFL61-Gateway (Desbrosses-Fonrouge et al., 2005) using LR clonase (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... The hybridization buffer was composed of encoding wash buffer supplemented with 0.1% (wt/vol) yeast tRNA (Life Technologies), 1% (vol/vol ...
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bioRxiv - Genomics 2020Quote: ... SD media used for growth of yeast cultures contained: 2% w/v dextrose (Thermo Fisher Scientific, Waltham, MA), 13.4 g/L Yeast Nitrogen Base without Amino Acids (BD Biosciences ...
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bioRxiv - Molecular Biology 2022Quote: All plasmids used in yeast two-hybrid assays were cloned with the Gateway® Gene Cloning Technology (Invitrogen) and transformed in the AH109 and Y187 haploid yeast strains (Clontech) ...
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bioRxiv - Neuroscience 2022Quote: ... each slide was covered in 300 μl of a hybridization buffer containing yeast tRNA (Invitrogen; Carlsbad, CA, USA), salmon sperm DNA (Ambion) ...
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bioRxiv - Cancer Biology 2021Quote: The yeast display selection process was started with two rounds of selections using streptavidin-coated dynabeads (Thermo Fisher) loaded with PXDN-con4-biotin ...
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bioRxiv - Immunology 2021Quote: ... Tp2 and Tp9 were expressed for either surface display in the pYD1 vector (Yeast Display Vector kit, Invitrogen) or for intracellular expression using the pYES2/NTC vector (N-terminal Xpress and C-terminal V5 epitope ...
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bioRxiv - Molecular Biology 2023Quote: ... were blocked for 2 hr at 4 °C in lysis buffer containing 1 mg/ml yeast tRNA (Ambion) and washed twice with 1 ml lysis buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... ceSMSγ and ceSMSr cDNAs were cloned into yeast expression vector pYES2.1/V5-His-TOPO and mammalian expression vector pcDNA3.1/V5-His-TOPO (Invitrogen) as described previously(18).For expression studies in Drosophila S2 cells ...