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Citations for Thermo Fisher :
451 - 500 of 10000+ citations for Calponin 1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Virus infected cells were detected by staining with a primary rabbit anti-VACV antibody (1:1000 in blocking buffer for 1 hour, Thermo Fisher PA1-7258) and a secondary horseradish peroxidase-conjugated goat anti-rabbit antibody (1:1000 in blocking buffer for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... cells were incubated for 1 h with 488 (FLAG) and 594 (LCMV NP) Alexa Fluor antibodies (Life Technologies; 1:500 in blocking buffer), then washed four times with PBS.
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bioRxiv - Cell Biology 2023Quote: ... and anti-N-WASP (Cell Signalling #4848S; 1:100) followed by Alexa Fluor 568 conjugated secondary antibodies (Invitrogen; 1:1000 in blocking buffer). Actin tails were labeled with Alexa Fluor 488 phalloidin (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated with a cocktail of secondary antibodies (1:500, Alexa Fluor 488, 546, 647) in blocking solution containing Hoechst 33342 (1:10,000, Life Technologies, Cat. No. H3570) overnight at room temperature followed by three final washes with PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 mL min-1) and identity of peptides confirmed by high-resolution mass (Q Exactive Orbitrap LC-MS/MS (Thermo Fisher Scientific).
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bioRxiv - Immunology 2024Quote: ... A polyclonal antibody against the ROTR-1 protein was raised using the peptide LDRSPPSDDGTQKV (ROTR-1 amino acids 288 to 301) in rabbit (Thermo Fisher Scientific). Anti-ROTR-1 sera was used at a dilution of 1:1000 ...
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bioRxiv - Cell Biology 2022Quote: ... Blocking Buffer (E-cadherin mAb #14472 Cell Signaling Technology 1:1000, β-catenin mAb #8480 Cell Signaling Technology 1:1000, Occludin mAb #33-1500 Invitrogen 1:500). On the next day the membranes were washed three times for tenminutes in Tris-buffered saline + 0.05% Tween (#91414 ...
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bioRxiv - Cell Biology 2021Quote: ... Fixed cell layers were washed gently with PBS once before addition of endogenous biotin blocking solutions (Endogenous Biotin Blocking kit, Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... Peptides were resuspended in 0.1% TFA 2% ACN and quantified using Pierce™ Quantitative Fluorometric Peptide Assay (Thermo Fisher Scientific). Equal portions of all samples were mixed together to make a reference sample to be run multiple times for chromatogram library runs.
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bioRxiv - Cancer Biology 2022Quote: ... samples were resuspended in 0.1% FA and peptides were quantified by Pierce™ Quantitative Fluorometric Peptide Assay (Thermo Fisher Scientific). 500 ng of peptides of each sample were analyzed by Liquid Chromatography-Mass Spectrometry (LC-MS).
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bioRxiv - Microbiology 2022Quote: Peptides were fractionated into eight fractions using the high pH reversed-phase peptide fractionation kit (Pierce™ Thermo Fisher Scientific) according to the manufacturer’s instructions and using self-made columns as previously described48 ...
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bioRxiv - Cancer Biology 2021Quote: Two-dimensional fractionation—Peptides were fractionated using a Pierce™ High pH Reversed-Phase Peptide Fractionation Kit (Thermo Scientific, 84868) according to the manufacturer’s instructions with slight modifications ...
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bioRxiv - Physiology 2021Quote: ... Peptide concentration was measured with a colorimetric assay (Pierce Quantitative Colorimetric Peptide Assay kit, Thermo Fisher Scientific, Rockford, IL, USA).
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bioRxiv - Immunology 2020Quote: ... the HLA-A*02:01-restricted KRASwt (KLVVVGAGGV) and KRASG12D (KLVVVGADGV) aa5-14 peptides were synthesized (Peptide 2.0) and analyzed on an Orbitrap Fusion (Thermo Scientific) mass spectrometer (MS ...
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bioRxiv - Immunology 2022Quote: ... The Hc gene with a N-terminal gp67 signal peptide and Lc gene with a N-terminal honeybee melittin signal peptide were subcloned into pFastBac Dual vector (Invitrogen) containing two multiple cloning sites ...
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bioRxiv - Immunology 2022Quote: ... MBP peptide NPVVHFFKNIVTPR and influenza hemagglutinin PRFVKQNTLRLAT peptide were labeled with Alexa Fluor 488 (Alexa488) tetrafluorophenyl ester (Invitrogen, Carlsbad, CA) and used as probe peptides for DP4.1 ...
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bioRxiv - Biochemistry 2023Quote: ... Tryptic peptide mixtures including the stable-isotope labelled standard peptides were analysed on a nano-HPLC (Easy nLC, Thermo Scientific) coupled to an Orbitrap mass spectrometer (LTQ-Orbitrap ...
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bioRxiv - Biochemistry 2023Quote: ... HM and CM peptides resulting from the previous step were desalted using peptide desalting spin columns (Thermofisher Scientific, Brisbane, Australia), according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... solubility of 19F peptide library was estimated by fluorescence spectroscopy using Pierce Quantitative Fluorometric Peptide Assay (Thermo Fisher Scientific, USA), and 0.5 mg/ml solution in DMSO was used as a standard ...
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Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelopebioRxiv - Cell Biology 2023Quote: ... The mixture of TMT-labeled peptides was acidified and fractionated using a high pH reversed-phase peptide fractionation kit (ThermoFisher) according to the manufacturer’s recommendation ...
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bioRxiv - Immunology 2023Quote: Phosphorylated peptides were enriched from 10uG total peptides using a High-Select Fe-NTA Phosphopeptide Enrichment Kit (Thermo Scientific A32992), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... The concentration of tryptic peptides was measured using the Pierce™ Quantitative Peptide Assays kit (Thermo Fisher Scientific cat# 23275), and 25 µg of peptides were separated on a home-made reverse-phase C18 column in a pipet tip as previously (38) ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were washed three times in 1X PBS and then incubated with the appropriate PLA-antibodies (PLAminus, PLAplus, Duolink, 1:10) diluted in blocking buffer and a secondary antibody to visualize MAP2 (A488, ThermoFisher, A-11073, 1:1000) for one hour in a pre-warmed humidified chamber at 37 °C ...
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bioRxiv - Developmental Biology 2023Quote: ... in 1x PBS and blocked for 1 hour (h) in blocking buffer made of washing buffer and 1% heat-inactivated goat serum (HIGS) (Life Technologies, Waltham, MA, USA). Skin sections were fixed in 4% PFA for 10 min before they were washed and blocked in blocking buffer containing 10% HIGS ...
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bioRxiv - Neuroscience 2023Quote: ... sections were blocked with Mouse-on-Mouse IgG Blocking Solution for 1 h at room temperature (1:30; Thermo Fisher Scientific, Catalog no. R37621). Sections were incubated overnight at 4°C with primary antibodies—rabbit anti-Iba1 (1:1000 ...
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bioRxiv - Microbiology 2020Quote: 3 ⨯106 cells were washed with FACS buffer (0.2% BSA, 1 mM in PBS) before blocking with Fc block (Invitrogen) and incubation with eF780 live/dead viability stain (eBioscience ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were then incubated in the blocking/0.5 % BSA solution containing 1:500 of a secondary antibody (goat anti-chicken, Alexa Fluor 488, Invitrogen, # A-11039 ...
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Dual functionality of the TasA amyloid protein in Bacillus physiology and fitness on the phylloplanebioRxiv - Microbiology 2019Quote: ... the membranes were probed with anti-TasA antibody (rabbit) used at a 1:20,000 dilution in Pierce Protein-Free (TBS) blocking buffer (ThermoFisher). For immunodtection of FloT-YFP ...
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bioRxiv - Microbiology 2020Quote: ... Cells were then rinsed for 5 min at RT (3X) with PBS and incubated with secondary antibody (Alexa Fluor 647 diluted 1:2,000 in blocking buffer; Invitrogen) for 45 min at RT ...
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bioRxiv - Developmental Biology 2021Quote: ... followed by incubation with secondary antibodies and conjugated antibodies in blocking buffer (SOX2-GFP, 1:500, eBioscience, cat.no.53-9811-82; Donkey anti-mouse, AF647, ThermoFisher, cat ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were then incubated with Alexa Fluor 647-conjugated donkey anti-rabbit antibody in blocking solution (1:500, Invitrogen) for 1 h in darkness and washed with PBS as described above.
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bioRxiv - Immunology 2021Quote: ... Blots were incubated overnight at 4°C with agitation in blocking buffer and anti-HisG-HRP (1:5000, Invitrogen), or anti-V5-HRP (1:5000 ...
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bioRxiv - Microbiology 2021Quote: ... the coverslips were then stained with an Alexafluor488 goat anti-rabbit antibody (Invitrogen, diluted 1:1000 in blocking buffer).
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bioRxiv - Cancer Biology 2022Quote: ... were diluted 1:5000 in 5 % BSA in TBST or in blocking buffer (Cat. no. 37538, Thermo Fisher Scientific) in PBS (1:1 ...
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bioRxiv - Microbiology 2023Quote: ... Secondary antibodies were prepared in 5% goat serum in blocking buffer: goat anti-mouse 594 (AlexaFluor Invitrogen, 1:2000) and goat anti-rabbit 488 (AlexaFluor Invitrogen ...
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bioRxiv - Biophysics 2023Quote: ... Secondary antibody staining was performed for 1 hour at RT in blocking solution (AF647 anti-rabbit #A21244, Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... The plates were washed again and 100 µL/well of Goat anti Rabbit IgG-HRP (1:9,000 in blocking buffer) (Invitrogen) was added and incubated for one hour at room temperature ...
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Regulation of Diseases-Associated Microglia in the Optic Nerve by Lipoxin B4 and Ocular HypertensionbioRxiv - Molecular Biology 2024Quote: Secondary antibodies were diluted in blocking buffer at 1:2000 dilution (Alexa Fluor 594 and Alexa Fluor 488, Invitrogen), and tissues were incubated with a secondary antibody cocktail overnight at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... sections were washed in TBS then incubated in blocking buffer containing secondary antibodies (Alexa Fluor conjugates, 1:400, Invitrogen) for 3-4 h at room temperature ...
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bioRxiv - Biophysics 2022Quote: ... the peptides were loaded onto a C8 analytical column (1 mm i.d. × 50 mm length, Thermo Fisher Scientific) and separated with Agilent 1290 Infinity II UHPLC system ...
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bioRxiv - Systems Biology 2020Quote: ... were resuspended in peptide loading buffer (0.1% FA, 0.03% TFA, 1% ACN) containing 0.2 pmol of isotopically labeled peptide calibrants (ThermoFisher 88321). Peptide mixtures were separated on a self-packed C18 (1.9 μm ...
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bioRxiv - Bioengineering 2021Quote: ... Celsus Laboratories) was reacted with peptide-hydrazides using 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride (EDC, ThermoFisher Scientific) in 0.1 M MES [2-(N-morpholino)ethanesulfonic acid] buffer with 8 M urea (Sigma ...
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bioRxiv - Molecular Biology 2019Quote: ... The resultant peptides were run for 30 minutes on a Dionex LC and Orbitrap Fusion 1 (Thermo Scientific) for LC-MS/MS ...
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bioRxiv - Molecular Biology 2022Quote: ... 1/2 peptide were separated and analyzed with a Nano-HPLC (EASY-nLC1200, Thermo Fisher Scientific, Inc., USA) coupled to Q-Exactive MS (Thermo-Fisher ...
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bioRxiv - Genomics 2024Quote: ... around 1 μg of total peptides were analyzed on an EASY-nLC 1000 UHPLC system (Thermo Fisher Scientific) coupled via a 20 cm C18 column ID picofrit column (New Objective ...
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bioRxiv - Neuroscience 2021Quote: ... and immersed in blocking buffer (5% goat serum (Gibco), 2% bovine serum albumin (Sigma ...
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bioRxiv - Immunology 2020Quote: ... ILT-2 (CD85J) blocking antibody (clone HP-F1, ThermoFisher), CD155 blocking antibody (clone D171 ...
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bioRxiv - Cancer Biology 2019Quote: ... saturated with the Protoarray-Blocking Buffer (PA055, Life Technologies) supplemented with Synthetic Block (PA017 ...
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bioRxiv - Biochemistry 2020Quote: ... Membranes were incubated in blocking buffer (Thermo Scientific #37539) for 1 hour at room temperature before applying antibodies ...
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bioRxiv - Biochemistry 2021Quote: ... After 15 minutes of blocking (Thermofisher, 14-9161-73), cells were stained with ACVR1 primary antibody (R&D systems ...