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Citations for Thermo Fisher :
451 - 500 of 527 citations for Bordetella Pertussis Toxin Glycerol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Tubes had equal amounts of glycerol and 0.04 M phosphate buffer (pH 7.2–7.6) containing 1× antibiotic–antifungal mixture (Thermo-Fisher Scientific, Johannesburg, South Africa) (46,47) ...
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A human cancer cell line initiates DNA replication normally in the absence of ORC5 and ORC2 proteinsbioRxiv - Molecular Biology 2020Quote: ... Cells were resuspended in buffer A (10 mM HEPES [pH7.9], 10 % glycerol, 1 mM DTT, protease inhibitor cocktail [Thermo Fisher Scientific]). After adding 0.1% Triton X-100 ...
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bioRxiv - Molecular Biology 2021Quote: ... 25 μL of magnetic HA beads was washed twice in 1X PBS with 0.1% Tween 20 and then twice in binding buffer (50 mM Tris pH7.5, 150 mM NaCl, 1% IGEPAL CA-630, 5% glycerol, and protease inhibitors (Thermo Fisher #A32955): apoptin ...
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bioRxiv - Genomics 2020Quote: ... 1% Triton X-100 and 10% glycerol and resolved on 3–8% pre-cast gradient Tris-Acetate gels (Invitrogen, Waltham, MA). For mouse samples ...
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bioRxiv - Cell Biology 2020Quote: Cells were lysed in Pierce IP Lysis Buffer (25 mM Tris HCl pH 7.4, 150 mM NaCl, 1% NP-40, 1 mM EDTA, 5% glycerol) (Thermo Fisher Scientific) containing 1% protease and phosphatase inhibitors on ice and protein concentration was measured by BCA ...
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bioRxiv - Cell Biology 2021Quote: ... pH 7.5/ EDTA 2 mM/glycerol 10%/Triton X-100 1%/sodium deoxycholate 0.5%/SDS 0.2%) supplemented with protease inhibitor cocktail (Thermo Fisher Scientific, A32965) from 10-cm dishes ...
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bioRxiv - Molecular Biology 2022Quote: ... were washed three times with Buffer A (PBS pH 8.3, 5% glycerol, 1 mM DTT, 0.03% NP-40, Pierce™ Phosphatase Inhibitor Mini Tablets (Thermo Fisher Scientific)) and incubated with 2 µg of un ...
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bioRxiv - Molecular Biology 2022Quote: ... streptavidin particles with immobilized peptides were washed three times with buffer B (PBS pH 8.3, 5% glycerol, 1 mM DTT, 0.1% NP-40, Pierce™ Phosphatase Inhibitor Mini Tablets (Thermo Fisher Scientific), Roche Complete Mini EDTA-free protease inhibitor tablets (Sigma-Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... cells were treated with GLP for 48 h and harvested and lysed with assay buffer (Tris 62.5 mM, pH 6.8, DTT 50 mM, 10%SDS, glycerol; Thermo Fisher Scientific, USA). Proteins were extracted and quantified using Bradford assay and separated by 15% SDS-PAGE and transferred onto a polyvinylidene fluoride membrane at 4°C (100 V) ...
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bioRxiv - Molecular Biology 2022Quote: ... and dialyzed at 4°C overnight with dialysis buffer (20mM HEPES pH 8.0, 200mM NaCl, 10% Glycerol, 2mM MgCl2) using Slide-A-Lyzer Dialysis cassette (Thermo Scientific 66383). 0.05% was included in all buffers when purifying Dll ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were washed and mounted in media (0.5% N propyl gallate, 70% glycerol, 1M tris pH8.0) containing 4′,6-diamidino-2-phenylindole (DAPI) (Invitrogen, Cat. No. D1306).
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bioRxiv - Physiology 2022Quote: ... washed once with cold acetone and then dissolved in Laemmli buffer (Tris 10 mM pH 7.5, EDTA 1 mM [Fluka, Buchs, Switzerland], β-mercaptoethanol 5%, SDS 5%, glycerol 10% [ThermoFisher Scientific]). Sonication and centrifugation were repeated as above to pellet and eliminate possibly remaining cell debris ...
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bioRxiv - Biochemistry 2020Quote: ... and resuspended in 40 μL 1x SDS sample buffer (75 mM Tris-HCl pH6.8, 10% glycerol, 2.5% SDS, 10% (v/v) Bond-Breaker TCEP Solution (Thermo Fisher, Cat# 7772), and 0.02% (w/v ...
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bioRxiv - Microbiology 2021Quote: ... magnetically isolated for a final time and then boiled in Laemmli buffer (62.5 mM Tris-HCl, 10% glycerol, 2% sodium dodecyl sulfate and 0.01% bromophenol blue (B392, Fisher Scientific, Pittsburgh, PA) for 5 minutes to release antibody ...
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bioRxiv - Bioengineering 2020Quote: ... bacterial suspensions for each strain were prepared by diluting the starter culture in minimum medium M63 supplemented with 20% glycerol (J910500G, Fisher Scientific) to 1:10 dilutions for E ...
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bioRxiv - Bioengineering 2021Quote: Inoculated starter cultures of 6mL LB-kanamycin that were shaker incubated at 37C 250rpm overnight were used to make 25% glycerol stocks and inoculate in a 1:50 ratio TB-kanamycin in baffled 1L flasks (Thermo Fisher). Cultures were shaker incubated at 37C 250rpm until OD600 (Nanodrop ...
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bioRxiv - Cell Biology 2020Quote: ... ECs were lysed on ice with MLB (Sigma) (diluted to 1X with sterile water containing 10% glycerol and 1X HaltTM protease inhibitor cocktail (Thermo Scientific). EC lysates were cleared by centrifugation ...
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Metal transporter SLC39A14/ZIP14 modulates regulation between the gut microbiome and host metabolismbioRxiv - Physiology 2021Quote: ... the pellets were resuspended in lysis buffer (Tris-HCl, 137mM NaCl, 10% glycerol, 1% Triton X-100, 2 mM EDTA) with protease and phosphatase inhibitors (Thermo Scientific) added along with the PMSF (Sigma-Aldrich) ...
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bioRxiv - Biophysics 2021Quote: ... movies of frozenhydrated apoferritin and aldolase in the presence of 20% glycerol were collected using a Titan Krios TEM (Thermo Fisher) with a field emission gun operating at 300 keV ...
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bioRxiv - Biochemistry 2022Quote: ... Purified Complex I was concentrated to ~2 mg/mL following size exclusion chromatography and glycerol was removed with a Zeba Spin desalting column (ThermoFisher Scientific). Grids were glow-discharged for 2 min in air immediately prior to application of 2 μL of sample to each grid ...
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bioRxiv - Biochemistry 2022Quote: ... proteins stored in Tris buffer were dialyzed four times against methylation buffer (25 mM HEPES [pH 7.5], 200 mM NaCl, and 10% glycerol) using Slide-ALyzer™ Dialysis Cassettes (Thermo Scientific) with an appropriate cut-off ...
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bioRxiv - Synthetic Biology 2023Quote: ... The harvested yeast pellets were also resuspended with the sterile frozen competent cells (FCC) solution (5% v/v glycerol (Fisher Scientific) and 10% v/v DMSO (Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... and 10% glycerol by dialysis using Slide-A-Lyzer G2 Dialysis Cassettes with 20-kDa cutoff (Thermo Fisher Scientific, no. 87737) at 4 °C overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... For males and a portion of the female samples: plasma TG and cholesterol were measured using colorimetric kits (note that the TG measurements include free glycerol) (Inifinity, Thermo Scientific, TG catalog #TR22421 ...
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bioRxiv - Neuroscience 2023Quote: ... samples were allowed to sink in 50% glycerol and then mounted on a glass slide and coverslip with SlowFade™ Gold Antifade Mountant (ThermoFisher). Slides were stored at 4°C in the dark until imaging on a Leica SP8 confocal microscope for all images except those in Figure 4B ...
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bioRxiv - Pathology 2023Quote: ... Rhabdomyolysis-AKI (rhabdo-AKI) was induced in male mice aged 13-14wk were given intramuscular (IM) 50% glycerol (Invitrogen, Carlsbad, CA) in sterile water after water deprivation for 18hrs prior to injection ...
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bioRxiv - Cancer Biology 2023Quote: ... or 647 were stored at -20 °C diluted 1:1 in glycerol and used at 1:500 dilution in Abdil for 1 hour at room temperature (Invitrogen Molecular Probes A11029 ...
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bioRxiv - Biochemistry 2024Quote: ... 200 mM NaCl, 0.08% Triton X-100, 25% glycerol, 20 mM reduced glutathione, 1 mM DTT, and 1X synthetic block [Invitrogen cat# PA017]) for one hour with gentle rocking ...
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bioRxiv - Cancer Biology 2024Quote: ... Sample concentrations were normalized using 2X SDS sample buffer (62.5 mM Tris pH 6.8, 2% SDS, 10% glycerol (Fisher Scientific G33-500), bromophenol blue (Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... We set a wait time of 120 seconds and applied 3 µl of a solution containing 10% glycerol in HBSS (ThermoFisher, Germany). The grids were then backside blotted for 30 seconds with blot force 20 and subsequently plunged into liquid ethane at melting point ...
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bioRxiv - Biochemistry 2024Quote: ... 25% glycerol and 0.05% bromophenol blue] and were separated on 4 -16% Native PAGE Bis-Tris gradient gels (Thermo Fisher Scientific) (44) ...
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bioRxiv - Evolutionary Biology 2024Quote: Fluorescently labeled samples were mounted in glycerol mounting solution (80% glycerol, 0.1 M Tris, pH 8.0) on microscope slides coated with poly-L-lysine (Thermo Fisher Scientific #86010). Samples were imaged at ×40 on a Leica TCS SP8 confocal microscope ...
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bioRxiv - Developmental Biology 2024Quote: ... Tissue was mounted on glass slides in a small amount of 70% glycerol with a drop of SlowFade Diamond Antifade Mountant (ThermoFisher Scientific) and stored in the dark until imaging by confocal microscopy ...
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bioRxiv - Microbiology 2024Quote: ... As the local standard cryopreservative contains tryptone soya broth in addition to glycerol, samples of JKD8049 (prepared as shaking cultures and filtered, as described earlier) were tested using Veggietones (Oxoid, ThermoFisher Scientific) in glycerol ...
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bioRxiv - Cancer Biology 2024Quote: ... Sample concentrations were normalized using 6X SDS sample buffer (62.5 mM Tris pH 6.8, 5% SDS, 18% glycerol (Fisher Scientific G33-500), bromophenol blue (Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... isolates were revived from glycerol stocks into 500µL liquid LB-L media in wells of 2.0mL 96-well DeepWell™ blocks (ThermoFisher™ Nunc™). The blocks were incubated with shaking until the cultures reached stationary phase ...
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bioRxiv - Developmental Biology 2022Quote: ... DAB-developed samples were stored in 70% glycerol and counterstained with 4’,6-diamidino-2-phenylindole (DAPI, ThermoFisher Scientific, #D3571, 1:1000) nuclear marker ...
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bioRxiv - Physiology 2019Quote: ... to obtain images and then stored at 4°C in 5% acetic acid and 10% glycerol solution in a heat-sealable roll stock pouches (Fisher Scientific, #0181226E). Proteins in gels used for Western Blotting were then transferred to polyvinylidene difluoride membranes (Whatman) ...
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bioRxiv - Biochemistry 2021Quote: 10 uL of either Caspase-3 or −7 library glycerol stocks was used to inoculate 5 mL of auto-induction media (Invitrogen Magic Media) and allowed to incubate and express for 18 hours at 30 °C ...
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bioRxiv - Biochemistry 2020Quote: ... CryoEM specimens of ATP synthase without BDQ were prepared following removal of glycerol with a Zeba Spin desalting column (Thermo Fisher Scientific) with protein (2.5 μL ...
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bioRxiv - Biophysics 2021Quote: ... movies of frozen-hydrated aldolase and apoferritin in the presence of 20% glycerol were collected using a Talos Arctica TEM (Thermo Fisher Scientific) with a field emission gun operating at 200 keV ...
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bioRxiv - Microbiology 2020Quote: ... Bacterial stocks were maintained in 20% glycerol at −70°C and cultured on trypticase soy agar II with 5% sheep’s blood (SBA) (Fisher Scientific, Pittsburg, PA) at 35°C in ambient air for testing ...
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bioRxiv - Developmental Biology 2022Quote: ... All injection solutions were prepared in a 2.5 µl solution consisting of 20% glycerol and 0.4 μg/µl of 10,000 MW neutral non-fixable Texas Red dextran (Thermo Fisher Scientific, Waltham, MA). Approximately 1-2 picoliter was injected into each newly fertilized egg based on the size of the injection bolus at about one-fifth of the egg diameter ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... the cultured cells were lysed using SDS lysis buffer (100 mM Tris-HCl, pH 6.8, 10% glycerol, and 1% SDS) supplemented with a protease inhibitor cocktail (Thermo Fisher Scientific, 78441). The protein concentration was determined using the BCA protein assay kit (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... fillets were mounted in elvanol containing antifade (polyvinyl alcohol, Tris 8.5, glycerol and DABCO; DABCO, catalogue number 11247100, Fisher Scientific, Hampton, NH). The following antibodies were used on dissected larval fillets ...
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bioRxiv - Biochemistry 2021Quote: CryoEM grids of drug-free ATP synthase were prepared by removing glycerol from the sample with Zeba Spin Desalting Column (Thermo Fisher Scientific) immediately before freezing ...
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bioRxiv - Cell Biology 2021Quote: ... Elutions containing the highest concentration of purified nanobody were pooled and dialysed against 1xPBS + 20% glycerol using Slide-A-LyzerTM (Thermo Scientific B2162132) and stored in 1xPBS + 20% glycerol ...
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bioRxiv - Developmental Biology 2023Quote: Muscle injuries to the Tibialis Anterior (TA) were induced with 30 – 50µL of either 50% glycerol (GLY, Acros Organics, 56-81-5) or 30 – 50µL of 10µM cardiotoxin (CTX ...
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bioRxiv - Immunology 2023Quote: ... pH 6.8 buffer containing 2% SDS and 20% glycerol) with the addition of protease and phosphatase inhibitors (A32953, A32957 respectively, Thermo Fisher Scientific). Proteins were separated using SDS-PAGE under reducing conditions ...
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bioRxiv - Systems Biology 2024Quote: ... Cells were washed with 2x SSC and then switch to a photo-protective buffer containing 50% glycerol (Thermo Fisher Scientific, Waltham, MA), 75 μg/mL glucose oxidase (Sigma Aldrich ...