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Citations for Thermo Fisher :
451 - 500 of 10000+ citations for Anti Integrin beta 5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... or 5 μg anti-Foxp3 (eBioscience) antibody in the presence of DynaBeads IgG magnetic beads (50 μl/sample) (Thermo Fisher Scientific) overnight at 4℃ ...
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bioRxiv - Bioengineering 2023Quote: ... Slides were then rinsed three times for 5 min each with PBS-Tx and were incubated with goat anti-rabbit antibody conjugated with polymer HRP (Thermofisher B40962) for 45 minutes at room temperature ...
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bioRxiv - Immunology 2023Quote: ... or 5 μg anti-Foxp3 (eBioscience) antibody in the presence of DynaBeads IgG magnetic beads (50 μl/sample) (Thermo Fisher Scientific) overnight at 4℃ ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was blocked with PBS + 0.1% Tween 20 (PBS-T) and 5% nonfat milk prior to incubating in monoclonal anti-FLAG M2 antibody (1:40,000, Sigma, Thermo Scientific™). overnight at 4 °C followed by Goat-anti Mouse IgG-HRP secondary antibody (Invitrogen™ ...
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bioRxiv - Cell Biology 2023Quote: ... and followed the same procedure described above until the application of the secondary antibody solution (5% normal goat serum in PBST, Alexa Fluor 488 anti-guinea pig 1:200 (Thermo Scientific)) ...
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bioRxiv - Cell Biology 2023Quote: ... the transfected cells were blocked with 5% BSA at room temperature for 15 min and labeled with anti-FLAG antibody (1:100, Thermo Fisher) at 4°C for 1 h ...
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bioRxiv - Neuroscience 2024Quote: ... neurons were washed with PBS three times for 5 min and incubated with secondary antibodies (goat anti-rabbit-AlexaFluor488: 1:1000, Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... then with the following antibodies in 5% BSA for 1 h: donkey anti-rabbit IgG Alexa Fluor 488 (A21206, Thermo Fisher) and α-Bungarotoxin Alexa Fluor 594 (B13423 ...
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bioRxiv - Neuroscience 2024Quote: ... brains were then washed four times in 0.05% PBS-T and then incubated with 5% NGS and secondary antibody (1:400 Alexa 594 goat anti-mouse IgG, Invitrogen A11032) in 0.5% PBS-T for 2 days at 4 °C with nutation ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were then washed and incubated with the secondary antibody dilution (Horse anti-mouse, HRP-ligated, PI 2000, 1:5000 in 5%dry milk, Thermo Fisher) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... Blots were washed with 1xTBST or 1xTBS 3x quickly then 3x for 5 min before incubating with secondary antibodies Alexa Fluor 700 goat-anti-rabbit IgG (Invitrogen # A21038) or IRDye-800CW goat anti-mouse IgG (LI-COR # 926-32210 ...
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bioRxiv - Cancer Biology 2019Quote: ... Secondary antibodies were anti-mouse or anti-rabbit Alexa Fluor-conjugated antibodies (Invitrogen, Carlsbad, CA). Slides were counterstained with DAPI before visualization by fluorescence microscopy.
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bioRxiv - Neuroscience 2022Quote: ... Anti-phosphotau antibody AT8 and anti-tubulin antibody DM1A were purchased from ThermoFisher (Loughborough, UK) and used at 1/200 and 1/10,000 ...
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bioRxiv - Immunology 2022Quote: ... and anti-pp65 antibody (mouse, Argene) combined with goat anti-mouse antibody (AF488, Thermo Fisher), respectively.
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bioRxiv - Biochemistry 2020Quote: ... Goat-anti-mouse-Alexa 568 antibody and goat-anti-rabbit-Alexa 488 antibody (Molecular Probes) were generously provided by Dr ...
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bioRxiv - Cell Biology 2022Quote: ... anti-guineapig CENP-C antibody (MBL) followed by Alexa Flour 647 anti-guineapig antibody (Invitrogen). All antibodies were diluted at 1:500 dilution rate.
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bioRxiv - Microbiology 2023Quote: ... and a HRP-conjugated donkey anti-goat antibody anti-goat antibody (1:20,000) (Thermo Fisher). Loading controls were performed by using an anti-tubulin antibody (1:4.000 ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were then incubated in 5% normal donkey serum (NDS) for 1 hour before primary antibody incubation (mouse anti-GFP, 1:1500, Life Technologies; rabbit anti-TH, 1:2000, Millipore) overnight at room temperature with NDS and PBS-Tx ...
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bioRxiv - Genetics 2023Quote: ... cut horizontally to separate EZH2 and beta-actin and then incubated at 4°C with primary EZH2 antibody (ThermoFisher, Cat. No. #49-1043; 1: 1.000) and anti-beta actin antibody (Sigma-Aldrich-A2228-RRID:AB_476697 ...
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bioRxiv - Cell Biology 2020Quote: ... anti-mouse IgG2B or anti-rabbit antibodies (Life Technologies) were used for immunofluorescence ...
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bioRxiv - Immunology 2020Quote: ... A beta release of the Collibri 3’ mRNA Library Prep Kit (Invitrogen) was used to prepare libraries ...
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bioRxiv - Bioengineering 2023Quote: ... and housekeeping gene for beta tubulin (TUBB) (#Hs00742828_s1, ThermoFisher Scientific, Waltham, MA). TaqMan master mix (4444556 ...
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bioRxiv - Immunology 2022Quote: ... Pre-designed gene expression assay for beta-Actin (Applied Biosystems™, Mm01205647_g1) was used for normalization ...
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bioRxiv - Developmental Biology 2023Quote: ... 1% penicillin/streptomycin (Pen/Strep) and 0.5 μM beta-mercaptoethanol (Life Technologies). Medium was changed every second day and cells were passaged at 1:3 or 1:5 ratio ...
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bioRxiv - Bioengineering 2019Quote: ... cells were transfected with ThermoFisher Silencer Select siRNA targeting expression of β1 integrin (ThermoFisher, Catalog #4390824, siRNA ID s7575), or with a negative control (Silencer Select Negative Control No ...
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bioRxiv - Cancer Biology 2019Quote: shRNA sequences for ITGAV (integrin αv) and CD44 were obtained as glycerol stock in pTRIPZ inducible plasmids (Thermo Scientific). For transduction ...
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bioRxiv - Neuroscience 2023Quote: ... Cell suspension was incubated with Fc block antibodies for 5 minutes at 4°C and then stained with Thy1.2-FITC antibody and IgG2a isotype antibody (Thermo fisher) as a negative control for 30 minutes at 4 °C ...
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bioRxiv - Genetics 2020Quote: ... The antibodies used were anti-HA antibody (ThermoFisher #71-5500) in HUDEP-2 cells expressing an HA-tagged ZNF410 construct or anti-ZNF410 (Abcam #ab174204 ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary GR antibody (G-5, Santacruz sc-393232, 1:100) or MAP2 antibody (Invitrogen, 1:2000) incubations lasted for 1 h at RT followed by 3x washes and 1 h incubation in secondary antibody at RT (Cy3 Goat anti-mouse against GR ...
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bioRxiv - Cell Biology 2021Quote: ... anti-mouse-IgG antibodies and secondary Alexa-Fluor-680-conjugated goat anti-rabbit IgG antibodies (Thermofisher) were used ...
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bioRxiv - Neuroscience 2021Quote: ... the membranes were incubated with anti-Tyrosine Hydroxylase antibody (MilliporeSigma) or anti-GFP Polyclonal antibody (Thermofisher). After incubation with HRP-linked anti-rabbit IgG ...
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bioRxiv - Developmental Biology 2023Quote: ... Primary antibodies were detected using anti -rabbit- and anti-mouse-Alexa Fluor conjugated secondary antibodies (Invitrogen). Cell nuclei were stained with 4′ ...
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bioRxiv - Cancer Biology 2021Quote: ... Tubulin beta chain protein was detected with an anti-TUBB antibody (1:500 in 5% fat-free milk solution in TBST, Invitrogen MA5-16308) followed by an anti-mouse secondary antibody (Alexa Fluor™ Plus 800 ...
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bioRxiv - Immunology 2019Quote: ... lung tissues were incubated with primary antibodies diluted in PBS with 0.3% TritonX-100 on a rocker at 4° C overnight. Primary antibodies used (Fig. 5) were: rat anti-E-cadherin (E-Cad, 131900, Invitrogen; 1:1000), goat anti-CCSP (1:2,500 ...
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bioRxiv - Neuroscience 2019Quote: ... cells were washed three times with PBS for 5 min and further incubated with anti-mouse Alexa 555 antibody (1:500, Thermo Fisher Scientific). Finally ...
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bioRxiv - Molecular Biology 2021Quote: ... and then 25 μL of 20 mg/mL BSA and 5 μL of anti-V5 antibody (Invitrogen, Waltham, MA, Cat # R960-25) was added to the rest of the sample and rotated overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... the sections were washed with PBS and incubated with secondary antibody goat-anti-rabbit Alexa 488 (Mol. Probes, A32731) for 20 minutes and Hoechst 33342 for 5 minutes (Thermo Fisher, H3570). After washing with PBS ...
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bioRxiv - Plant Biology 2022Quote: ... or 5 μg of polyclonal anti-RpoB antibody (PhytoAB catalog number Phy1239) with 40 μl Protein G Dynabeads (Invitrogen catalog number 10004D) or 60 μl Protein A Dynabeads (Invitrogen catalog number 10002D ...
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bioRxiv - Cancer Biology 2022Quote: ... The CD4 and CD8 depleted cells were cultured in 24-well plates pre-coated with 5◻μg/ml anti-CD3 antibody (OKT3, eBioscience) for 3◻days in RPMI-1640 (Gibco) supplemented with 10% FBS (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... The specificity of this antibody has been previously validated by us and others by Western Blot, IF and IHC analyses (5, 6).The Anti-NeuN mouse monoclonal antibody (ThermoFisher MA5-33103) was used for IF ...
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bioRxiv - Cancer Biology 2019Quote: ... The blot was blocked for 1 h with 5% skimmed milk and the membrane was probed with anti-PLK1 primary monoclonal antibody (Invitrogen: 37-7000), anti-BCL2 primary monoclonal antibody (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were washed with 1X PBS 3 times for 5 minutes each and were incubated with Alexa Fluor 488 conjugated goat anti-rabbit antibodies (Catalog # A-11008, Invitrogen, Carlsbad, CA) diluted 1:1000 in antibody incubation buffer for 4 hours at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were washed again with PBS (3X, 5 min) and incubated with a goat anti-mouse Alexa 568 antibody (highly cross adsorbed) (Invitrogen, Carlsbad, CA) at a 1:1000 dilution in 1% BSA in PBS for 1 hr in the dark ...
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bioRxiv - Cell Biology 2022Quote: ... washed three times with PBS for 5 min and incubated with donkey anti-rabbit secondary antibody coupled to Alexa Fluor 488 (Invitrogen; 1:2,000). Coverslips were washed in PBS as above and mounted on glass slides using Fluoromount G Reagent (Southern Biotech) ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were washed three times in washing solution for 5 min at RT and incubated with secondary antibodies (donkey anti-rat Alexa Fluor 488 (1:200, A-21208, Thermo Fisher Scientific) and donkey anti-mouse Alexa Fluor 555 (1:200 ...
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bioRxiv - Immunology 2021Quote: ... Sections were washed 3 x 5 minutes in PBS and stained with secondary antibodies donkey anti-rabbit Alexa Fluor 555 (1/1000, Invitrogen, A-31572) and donkey anti-goat Alexa Fluor 488 (1/1000 ...
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bioRxiv - Developmental Biology 2022Quote: ... The membrane was washed 5 times 5 min in TBS-T prior to overnight incubation at 4°C with 1° anti-Flag antibody (1:500, ThermoFisher, PA1-984B). The next morning ...
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bioRxiv - Neuroscience 2022Quote: ... coverslips were washed three times with PBS for 5 minutes then stained with secondary antibody goat anti-rabbit AF488 (ThermoFisher, #A-11008) at 1:250 for 1 hour at room temperature in PBS + 0.1% Triton-X-100 ...
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bioRxiv - Plant Biology 2023Quote: ... The membranes were then blocked in 5% milk in PBS-T before being washed and probed with an anti-GFP11 antibody (Invitrogen: PA5-109258) (1:500 dilution in PBST with 3% BSA) ...
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bioRxiv - Neuroscience 2023Quote: ... The next day RGCs were washed 3 × 5 minutes with PBS and incubated with secondary antibodies: goat anti-mouse Alexa 488 (Thermo Fisher A11029) 1:1000 ...