Labshake search
Citations for Thermo Fisher :
451 - 500 of 10000+ citations for Adenovirus Type 5 Particles CMV β galatosidase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2021Quote: ... DRGs were enzymatically dissociated at 37°C for 2 × 20min in the HBSS solution supplemented with calcium (CaCl2 5 mM) and containing collagenase type II (Gibco, France) and dispase (Gibco ...
-
bioRxiv - Neuroscience 2019Quote: Cultured DRG neurons from wild-type and TRESK KO mice were loaded with 5 μM fura-2/AM (Invitrogen, Carlsbad, CA) for 45-60 min at 37°C in culture medium ...
-
bioRxiv - Cell Biology 2021Quote: ... 15 × 104 HEK 293 cells were seeded on collagen-coated coverslips (Cellmatrix, Type IV, Nitta gelatin, Osaka, Japan) and transfected with 5 nM siRNA using RNAiMax (Thermo Fisher) using a reverse-transfection protocol ...
-
bioRxiv - Cell Biology 2020Quote: ... a 4 hour enzymatic digestion into single cells was performed at 37°C in 35 mls HBSS containing 5 mg/ml collagenase type I (Life Technologies), 10 μM ROCK inhibitor Y-27632 (StemRD) ...
-
bioRxiv - Genomics 2022Quote: ... two-thirds of the cells were collected for single-cell sequencing and the remaining cells were re-plated on 6-well plates coated with 5 μg/cm2 Type I rat collagen (Gibco, A1048301). Two additional samples were collected on Days 11 and 21 for single-cell sequencing ...
-
bioRxiv - Biophysics 2023Quote: 12-well cultures of Hep-2 cells at 70% confluence were transfected with 0.5 μg of wild-type or mutant UL34 or UL31-FLAG plasmid using Lipofectamine as described by the manufacturer (Gibco-BRL) and incubated at 37 °C overnight ...
-
bioRxiv - Cell Biology 2023Quote: ... Assorted tissues from third instar larvae (L3 stage) of the wild type Canton-S strain were dissected during 2-5 min in PBS (Thermo Fisher) with or without 10 % glycerol after turning larvae inside out with the help of fine tip forceps ...
-
bioRxiv - Biophysics 2020Quote: The engineered Cys residue in β or PCNA mutants was covalently modified with tetramethylrhodamine-5-maleimide or Alexa Fluor 546 C5 maleimide (ThermoFisher) and purified from the free fluorophore as described previously (9,10) ...
-
bioRxiv - Biochemistry 2020Quote: ... Samples were analyzed by SDS-PAGE under reducing conditions (5% β-mercaptoethanol) on 4-12% Bis-Tris NuPage Gels (Thermo Fisher) and cleavage products were detected using mouse monoclonal BC-3 antibody which detects aggrecan cleavage at the Glu392↓Ala393 bond (Cat n. ...
-
SARS-CoV2 associated secretion of nanoLuciferase reports on virus and Virus-Like Particle productionbioRxiv - Molecular Biology 2022Quote: ... with 5% β-mercaptoethanol at 50°C for 10mins and loaded onto a 4-20% Tris-glycine SDS-PAGE gel (Invitrogen). The proteins were transferred to a nitrocellulose membrane with an iBlot (ThermoFischer) ...
-
bioRxiv - Cell Biology 2020Quote: ... Synthetic oligos were designed to clone into pKGG (small cargo) and pRC/CMV (Invitrogen) with myc-pyruvate kinase (large cargo) ...
-
bioRxiv - Molecular Biology 2021Quote: ... 3.2 µg of pFlag-CMV-Ago2 plasmid were transfected using Lipofectamine 2000 (Invitrogen, #11668019). Cells were harvested three days after transfection.
-
bioRxiv - Biochemistry 2023Quote: ... and 1 ng of CMV-Rluc reporter plasmids (78) using Lipofectamine 3000 (Invitrogen L3000015).
-
bioRxiv - Genetics 2019Quote: ... Total RNA was extracted from the heads of 5 bees of each type (Scharlaken et al., 2008) using 500 μL of TRIzol® Reagent (Life Technologies) for each sample according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2019Quote: ... immortalized wild type MEFs (Heale et al., 2006) and 293T cells were cultured at 37°C and 5% CO2 in DMEM (Gibco 31600-034) supplemented with 10% fetal bovine serum and penicillin-streptomycin (50U/mL).
-
bioRxiv - Cancer Biology 2020Quote: ... Then 100 mg of tumor was collected and isolated in single cells by the buffer containing 5 mg/ml collagenase type IV (Gibco, 17104-019) and 1 mg/ml dispase II (Gibco ...
-
bioRxiv - Neuroscience 2023Quote: Primary microglial cultures were prepared using whole brains of P0-P1 C57BL/6J wild-type or SorLA-KO newborns as described (5) and cultured in DMEM w/Glutamax (Gibco, #10569-010) with 10% FBS (Pan Biotech ...
-
bioRxiv - Microbiology 2020Quote: ... The viral particle concentration was determined by Qubit fluorometric quantification (ThermoFisher) against a DNA standard (1 µg = 2.34E+10 virions) ...
-
bioRxiv - Biochemistry 2019Quote: ... 3 µm particle size (Thermo Fisher Scientific Inc., Waltham, MA U.S.A.) and separated using a 130 min gradient (100 min from 0% −35% Buffer B ...
-
bioRxiv - Immunology 2021Quote: ... Virus particles in 2% FBS/DMEM/1mM Pen/Strep (Thermofisher, 15140122) were incubated with cells for 2 hours at room temperature ...
-
bioRxiv - Microbiology 2021Quote: ... cells and purified virus particles were lysed with RIPA (ThermoFisher Scientific) buffer supplemented with complete protease inhibitor cocktail (Roche ...
-
bioRxiv - Microbiology 2022Quote: ... Equal MV particles were labelled with R18 (R18, ThermoFisher Scientific O246) and bound to HeLa cells or blebs and the pH maintained at 7.4 or lowered to 5.0 by the addition of 100 mM MES. ...
-
bioRxiv - Microbiology 2023Quote: ... on the KingFisher Flex Magnetic Particle Processor (ThermoFisher Scientific, Waltham, MA), according to manufacturer’s protocols ...
-
bioRxiv - Cancer Biology 2023Quote: Lentiviral particles were produced by transfecting 293FT cells (Thermo Fisher, R70007) with the pBA439_UMI20 library ...
-
bioRxiv - Molecular Biology 2023Quote: ... 30 µl of Dynabeads Protein G magnetic particles (Thermo Fisher Scientific) per ml of total lysate were washed twice with 1 mL of PBS-Tween (0.02%) ...
-
bioRxiv - Molecular Biology 2023Quote: Virus particles were fixed in 1 % glutaraldehyde (Cat# 233281000, Thermo Scientific). A 4 µL aliquot of sample was adsorbed onto holey carbon-coated grid (Lacey ...
-
bioRxiv - Biochemistry 2024Quote: ... column (Betasil C18, 100 × 2.1 mm, 3 µm particle; Thermo Scientific) was washed with 100 mL of Solvent B (MeOH ...
-
bioRxiv - Microbiology 2023Quote: ... and hydrophobic (Sera-Mag Speedbead carboxylated-modified particles, Thermo Fisher Scientific) magnetic beads ...
-
bioRxiv - Bioengineering 2023Quote: ... 2.4 μm particle size (Thermo Scientific, Waltham, MA, USA, 28102–152130). A mobile phase of A ...
-
bioRxiv - Cell Biology 2020Quote: ... and 0.1% type II collagenase (Invitrogen) in Dulbecco’s modified Eagles medium (DMEM ...
-
bioRxiv - Developmental Biology 2022Quote: ... (Type IV, Thermo Fisher Scientific, 17104019) and 0.05% Trypsine EDTA (Thermo Fisher Scientific ...
-
bioRxiv - Developmental Biology 2022Quote: ... (Type IV, Thermo Fisher Scientific, 17104019) and 0.05% Trypsine EDTA (Thermo Fisher Scientific ...
-
bioRxiv - Developmental Biology 2022Quote: ... (Type IV, Thermo Fisher Scientific, 17104019) and 0.05% Trypsine EDTA (Thermo Fisher Scientific ...
-
bioRxiv - Developmental Biology 2022Quote: ... (Type IV, Thermo Fisher Scientific, 17104019) and 0.05% Trypsine EDTA (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2022Quote: ... 1.2mg/mL collagenase type II (Gibco), and calcium chloride (Sigma ...
-
bioRxiv - Bioengineering 2021Quote: ... Type I agarose (Fisher scientific, UK) was added to distilled water and autoclaved at 121 °C to make 2% ...
-
bioRxiv - Immunology 2020Quote: ... Type IV Collagenase was from Gibco. DNase I was from Applichem ...
-
bioRxiv - Physiology 2019Quote: ... containing 0.2% type II collagenase (Gibco) for one hour at 37°C with constant agitation ...
-
bioRxiv - Immunology 2022Quote: ... 37.5 mg collagenase type II (Gibco), 1 mL PBS ...
-
bioRxiv - Neuroscience 2023Quote: ... and Dispase type II (ThermoFisher Scientific) for 15 minutes at 37°C ...
-
bioRxiv - Cancer Biology 2023Quote: ... 1.2mg/mL collagenase type II (Gibco), and calcium chloride (Sigma ...
-
bioRxiv - Immunology 2024Quote: ... 37.5 mg collagenase type II (Gibco), 1 mL PBS ...
-
bioRxiv - Developmental Biology 2021Quote: ... normalized to β-galactosidase activity using Galacto-Star β-Galactosidase Reporter (T1012, Invitrogen, Carlsbad, CA, USA) as described (Chen et al. ...
-
bioRxiv - Developmental Biology 2020Quote: ... normalized to β-galactosidase activity using Galacto-Star β-Galactosidase Reporter (T1012, Invitrogen, Carlsbad, CA, USA) as previous described (Chen et al. ...
-
bioRxiv - Neuroscience 2021Quote: ... and β-actin (Mouse Monoclonal anti-β-actin antibody Ambion, Thermo Fisher Scientific; Waltham, MA, USA).
-
bioRxiv - Systems Biology 2020Quote: ... isopropyl β-D-thiogalactopyranoside (IPTG) and ortho-nitrophenyl-β-galactoside (ONPG) were obtained from Fisher Scientific; methyl-β-D-thiogalactoside (TMG ...
-
Turanose induced WOX5 restores symbiosis in the Medicago truncatula cytokinin perception mutant cre1bioRxiv - Plant Biology 2020Quote: ... and immersed and incubated in the dark in staining solution 1 mM 5-bromo-4-chloro-3-indolyl-β-D-glucuronicacid (X-Gluc, Thermo Scientific), 50mM sodium phosphate buffer ...
-
bioRxiv - Genomics 2019Quote: ... boiled in 2X NuPAGE LDS Sample Buffer (including 1.8% β-Mercaptoethanol) for 5 min and separated in NuPAGE 4-12% Bis-Tris Protein Gels (Thermo Fisher Scientific).
-
bioRxiv - Molecular Biology 2023Quote: ... 7H11 plates contained 50 μg/mL hygromycin and 50 μM 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-gal) (Thermo Scientific). Unless specified ...
-
bioRxiv - Immunology 2021Quote: ... and 0.05 mM β-mercaptoethanol (Gibco) before co-culture with thymocytes ...