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Citations for Thermo Fisher :
451 - 500 of 10000+ citations for 6 Methoxy 3 methyl 1H indazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Cells were pulsed for 1h with 10μM EdU (Click-It Plus EdU Flow Cytometry Assay kit, Invitrogen #C10632) prior to collection ...
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bioRxiv - Neuroscience 2024Quote: ... Then the membrane was incubated for 1h at RT with appropriate horseradish peroxidase (HRP)-conjugated secondary antibodies (Invitrogen). The visualization of the signal was conducted using Immobilon Forte Western HRP substrate (Millipore ...
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bioRxiv - Immunology 2024Quote: ... incubated for 1h with either horseradish peroxidase (HRP)-conjugated goat anti-human IgM or IgG (Invitrogen, Camarillo, CA), washed again ...
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bioRxiv - Molecular Biology 2024Quote: ... and incubated for 1h at RT with secondary antibodies (Alexa Fluor conjugated produced in goat by Thermo Fisher) diluted in the blocking solution ...
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bioRxiv - Cell Biology 2024Quote: ... After fixing for 1h at 4°C in 4% PFA (prepared in PBS; Thermo Fisher Scientific, catalog 043368.9M), embryos were rinsed with PBS and then cryoprotected in 30% sucrose in 0.1M phosphate buffer overnight at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... Prior to all measurements cells were starved in FCS and phenol red free medium for 1h (Gibco, 31053028). For sialidase treatment ...
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bioRxiv - Cell Biology 2020Quote: ... Harvested equine explants were allowed to recover in 6-well plates for 3-7 days in the following control medium: DMEM/F12 medium (11330, Thermo Fisher Scientific, Waltham, MA), 10% fetal bovine serum (Seradigm 1500-500 ...
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bioRxiv - Developmental Biology 2020Quote: ... (N-((6-(2,4-DNP)Amino)Hexanoyl)-1-(BODIPY® F C5)-2-Hexyl-Sn-Glycero-3-Phosphoethanolamine) was purchased from Molecular Probes (Melbourne, VIC, Australia). The TiO2 was collected from a disassembled column ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Cells were seeded in 6-well plates (5×105/well) were transfected with the indicated siRNA (3 μg) using Lipofectamine® 2000 transfection reagent (Life Technologies, 11668-019) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Peptides were loaded onto a C18 pre-column (3 μm, 75 mm i.d. × 2 cm, 100 Å, Thermo Fisher Scientific Cat# 16-494-6) then separated on a reverse-phase nano-spray column (2 μm ...
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bioRxiv - Genomics 2024Quote: ... HAECs at low passage (passage 3-6) were treated prior to harvest every 2 days for 7 days with either 10 ng/mL TGFB2 (ThermoFisher Scientific, cat. no. 302B2002CF), IL1B (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2021Quote: ... a 6 well plate containing 6 mL FACS Clean (Thermo Fisher, BD 340345), 6 mL FACS Rinse (Thermo Fisher ...
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bioRxiv - Cell Biology 2021Quote: ... and labelled with 0.7 μM 6-carboxyfluorescin dictate (6-CFDA; Thermo Fisher Scientific) for 30 minutes at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... high dose IL-6 stimulation received 100 ng/ml IL-6 (Gibco; PHC0066) or 100 pM acetic acid vehicle stimulation for either 3-or 24-hours and immediately collected for analysis ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were extracted and snap-frozen in chilled 2-methyl butane (Acros Organics, Fisher Scientific, Pittsburgh, PA). Tissue blocks were sectioned (20 μm ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were extracted and snap-frozen in chilled 2-methyl butane (Acros Organics, Fisher Scientific, Pittsburgh, PA). Tissue blocks were sectioned (20 μm ...
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bioRxiv - Cell Biology 2022Quote: ... A 1 ml aliquot was treated with 2 μM tetramethylrhodamine methyl ester perchlorate (TMRE) (Molecular Probes, USA) and incubated at 37°C for 30 min ...
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bioRxiv - Cell Biology 2021Quote: Mitochondrial membrane potential was tested using the fluorescent potentiometric compound tetramethylrhodamine methyl ester (TMRM, 20 nM; Invitrogen). Cells were incubated in DMEM without phenol red with 0.1% FBS and CsH (1 µM ...
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bioRxiv - Neuroscience 2020Quote: ... 1 mM EDTA plus PIC) with 20 mM thiol-reactive methyl-methane thiosulfonate (MMTS, Thermo Fisher Scientific) to block free cysteine residues ...
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bioRxiv - Cancer Biology 2020Quote: ... 100 μl of N-methyl-N-trimethylsilyl trifluoroacetamide (MSTFA, Pierce) with 1% trimethylchlorosilane (1% TMCS, Thermo Scientific) was added to react for 30 min in 60°C ...
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bioRxiv - Cell Biology 2023Quote: ... Mitochondria labeling was achieved by adding 2 µL of a tetramethyl rhodamine methyl ester (TMRM, ThermoFisher, T668) directly to the medium to achieve a final concentration of TMRM of 100 nM ...
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bioRxiv - Biochemistry 2023Quote: ... and 2) adding 25 μL of N-methyl-N-trimethylsilyltriAuoroacetamide (TMS) with 1% trimethylchlorosilane (Thermo Fisher Scientific) and incubated for 30 minutes at 60°C ...
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bioRxiv - Neuroscience 2022Quote: ... the brains were rapidly frozen in dry ice-cooled 2-methyl butane (Fisher Scientific, Hampton, NH, USA). Coronal sections of the VTA at 10⍰μm were obtained using a cryostat and mounted onto superfrost slides (Fisher Scientific Ltd ...
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bioRxiv - Physiology 2024Quote: ... samples were incubated with methyl methanethiosulfonate (MMTS, 1 mol/L, Cat. # 23011; Thermo Fisher Scientific, Dreieich, Germany) at 50°C for 30 minutes to block free thiols ...
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bioRxiv - Microbiology 2024Quote: ... then PEGylated in situ using the methyl-PEG-NHS-ester reagent MS(PEG)12 (#22685, ThermoFisher Scientific). PEGylation was performed to reduce preferred orientation ...
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bioRxiv - Microbiology 2024Quote: ... The extracted fatty acid methyl esters were analysed using gas chromatography (Trace GC Ultra, Thermo Scientific, Germany) coupled to a mass spectrometer (ISQ ...
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bioRxiv - Cell Biology 2024Quote: Cells were transfected and 48 later incubated for 40min with 100nM tetramethylrhodamine methyl ester (TMRM) (#T668, Thermofisher), a fluorescent lipophilic cation ...
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bioRxiv - Biophysics 2021Quote: ... at a ratio of 1:1.5:1.75:2 (FAM155A-3×FLAG:NALCN-1077-3×HA-GFP:UNC79-3×FLAG:UNC80-3×FLAG) using Lipofectamine 3000 (Thermo Fisher Scientific) and incubated for 40-48 hours ...
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bioRxiv - Microbiology 2022Quote: ... per well were distributed in Ibidi® µ-slide 8-well chambered coverslips and stained with 0.8 μM of the membrane specific fluorescent styryl dye N-(3-triethylammoniumpropyl)-4-(6-(4-(diethylamino) phenyl) hexatrienyl) pyridinium dibromide (FM4-64; Thermo Fisher Scientific, Waltham, MA, USA) in the presence of 0 μM (control) ...
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bioRxiv - Genomics 2021Quote: ... The embryoid medium was aspirated and 15 to 20 embryoid bodies were plated per well in 6-well plates and cultured in 3 mL hematopoetic medium (2 mM GlutaMax, 1x Antibiotic-Antimycotic (Thermo Fisher Scientific; Cat. No. 15240062), 55 mM 2-mercaptoethanol (BioRad ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Total RNA was extracted from 3 6-mm punches of dried blood spot per owl monkey using the mirVana microRNA isolation kit (Thermo Fisher Scientific, Cat No: AM1560) according to manufacturer”s instructions ...
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bioRxiv - Microbiology 2024Quote: ... DNA was isolated from all samples using both these lab types (n=6) and concentration was assessed using a Qubit 3 (Thermo Fisher Scientific, Wilmington, DE, USA). DNA was isolated from the sludge and carcass rinsate samples using the DNeasy Ultraclean Microbial extraction kit (Qiagen Inc. ...
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bioRxiv - Biophysics 2021Quote: ... HsTMEM120A was subjected to protease digestion with 1mg/ml proteinase K at 37°C for 1h (Thermo Scientific; EO0491). 0.2% SDS was added to the digestion solution to stimulate the activity of proteinase K ...
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bioRxiv - Neuroscience 2022Quote: ... following 1h incubation at RT with corresponding Alexa 488/Cy3/Cy5 coupled secondary antibodies (1:1000, Thermo Fisher Scientific). Cell nuclei were stained with DAPI ...
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bioRxiv - Microbiology 2022Quote: ... and incubated for 1h at RT with AlexaFluor488-conjugated donkey anti-mouse IgG secondary antibody (0.2 µg/ml; ThermoFisher) diluted PBS-HS ...
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bioRxiv - Biophysics 2022Quote: ... Samples were incubated for 1h with a filtered and sonicated solution of red fluorescent carboxylate-modified beads (FluoSpheres, Invitrogen) of 100nm (220nm filter ...
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Control of cortical cytoskeleton-membrane interaction by RhoA regulates peripheral nerve myelinationbioRxiv - Neuroscience 2021Quote: ... Teased fiber preparations were permeabilized in 0.25% TX-PBS for 30 min and blocked for 1h in 1% NGS/1% horse serum (Gibco)/1% HEPES/PBS ...
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bioRxiv - Neuroscience 2020Quote: ... the strips were incubated with goat anti-human IgG Fc HRP-conjugated secondary antibody for 1h (Thermo Fisher Scientific). The film was exposed following routine immunoblotting steps ...
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bioRxiv - Neuroscience 2022Quote: ... cells were exposed for 1h at RT to secondary antibodies: Goat anti-chicken AlexaFluor 488 (1:1000, ThermoFisher, A21131), Donkey anti-rabbit AlexaFluor 555 (1:1000 ...
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bioRxiv - Microbiology 2020Quote: ... for 1h at room temperature with gentle mixing followed by inactivation with 40U of SUPERase-In RNase inhibitor (Ambion). Cell extracts were passed through Sephacryl S400 spin columns (GE ...
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bioRxiv - Neuroscience 2021Quote: ... which were then blocked during 1h at RT with Superblock™ in PBS blocking buffer (Thermo fisher Scientific #37515) or PBS containing 0.1% Tween 20 and 3% BSA ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were washed three times within PBS and incubated for 1h at room temperature with the secondary antibody (Alexa-488 anti-mouse and anti-rabbit IgG, 1:2,000, Invitrogen; CY3 anti-rabbit ...
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bioRxiv - Immunology 2023Quote: ... Slides were washed twice in PBS-T for 5min and incubated for 1h with anti-rabbit AlexaFluor555 (Invitrogen, #A32794) or anti-rabbit AlexaFluor647 (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... Electrophoresis was carried out in MOPS buffer by applying a constant current of 50mA for 1h (Electrophoresis kit, Invitrogen). Tubulin concentrations were assessed either by using Coomassie staining or acetylated tubulin content ...
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bioRxiv - Neuroscience 2022Quote: ... eBiosciences) for 1h before sections were mounted in 0.1 M phosphate buffer and cover-slipped with Flouromount G (Invitrogen) mounting media ...
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bioRxiv - Physiology 2022Quote: ... Hearts were again washed trice in PBT (0.2% Triton X-100 in PBS) for 1h each and incubated in blocking solution (10% FBS (4133, Invitrogen), 1% BSA (A7030-10G ...
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bioRxiv - Neuroscience 2024Quote: ... eBiosciences) for 1h before sections were mounted in 0.1 M phosphat buffer and cover-slipped with Flouromount G (Invitrogen) mounting media ...
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bioRxiv - Neuroscience 2024Quote: ... The secondary antibodies were diluted according to the manufacturer’s instructions and incubated for 1h at RT (488-ms/rb; 555-ms/rt; 647-ms, 1:1000, Invitrogen). Nuclei were visualized using 0,1μg/ml DAPI (Sigma Aldrich) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... corresponding secondary antibodies were added for 1h at RT: (Alexa-594-conjugated goat anti-rabbit IgG (H+L) (Invitrogen), Alexa-594-conjugated goat anti-chicken IgG (H+L ...
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bioRxiv - Physiology 2024Quote: ... and incubated for 1h at RT with a chicken anti-mouse antibody conjugated to Alexa Fluor 647 (Life Technologies, Thermo Fisher ...