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Citations for Thermo Fisher :
451 - 500 of 10000+ citations for 5 Bromo 2 Tert Butyldimethylsilyl 4 Methylthiazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... DAPI (4’,6-diamidino-2-phenylindole) (D1306) was purchased from Invitrogen. XMU-MP1 (#22083 ...
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bioRxiv - Neuroscience 2024Quote: ... counterstained with 4′,6-diamidino-2-phenylindole (DAPI; Thermo Fisher Scientific) and mounted on to glass slides in FluorSave™(Millipore) ...
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bioRxiv - Microbiology 2024Quote: For cultures stained with 4′,6-diamidino-2-phenylindole (DAPI, Thermofisher), the dye was added to 100 μl of cells at a final concentration of 1 μg/ml and the mixture was incubated at 30°C with shaking at 800 rpm for 5 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... neurons were loaded with 4 µM Fura-2 AM (Molecular Probes) for 30 min at 37°C in HBSS (consisting of in mM ...
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bioRxiv - Neuroscience 2024Quote: ... DAPI (4′,6-diamidino-2-phenylindole, 3 uM final) (Invitrogen, D1306) was used to stain the DNA content of cells so that doublets and debris could be removed by sorting on the DAPI height vs DAPI area ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer (Gibco) and 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... Counterstaining was done using 4’,6-diamidino-2-phenylindole (Invitrogen, #D1306). All Images were captured using a Leica Stellaris 8 Confocal Microscope and LAS X software (Leica Microsystems ...
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bioRxiv - Immunology 2020Quote: ... then labelled with 2’,7’-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluoresceinacetoxymethyl ester (Life Technologies, UK). Neutrophils were then added to wells under normoxia or hypoxia ...
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bioRxiv - Cell Biology 2023Quote: ... Equal volume of SYTOX Green (5 μM final in HBSS without Ca+2/Mg+2, Invitrogen) was added to each sample and incubated in the dark for 10 min ...
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bioRxiv - Microbiology 2022Quote: ... 2 mL malt extract (2% w/w) in 4 mL screw neck vials (Fisher Scientific, Loughborough, UK) were inoculated with an agar piece of the G ...
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bioRxiv - Cell Biology 2024Quote: ... adipose tissue (2∼4 cm3) was washed twice with PBS solution (AQ) containing 2% penicillin-streptomycin (Invitrogen), and then cut to 1∼2 mm3 with scissors ...
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bioRxiv - Neuroscience 2023Quote: ... Nuclear stain: 4′,6′-diamidino-2-phenylindole dihydrochloride (DAPI, blue; 2 ng ml−1; Molecular Probes, USA). Sections were cover-slipped using ProLong Gold anti-fade reagent (Invitrogen ...
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bioRxiv - Biophysics 2023Quote: ... Cell nuclei were stained with 4’,6’-diamidino-2-phenylindole dihydrochloride (DAPI; 2 ng/ml; Molecular Probes). Stained sections were imaged using epifluorescence and deconvolution epifluorescence microscopy on an Olympus IX83 ...
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bioRxiv - Neuroscience 2024Quote: ... Cell nuclei were stained with 4’,6’-diamidino-2-phenylindole dihydrochloride (DAPI; 2 ng/mL; Molecular Probes) for 15 minutes ...
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bioRxiv - Microbiology 2022Quote: ... or BA.4/5 spike protein (4 μg/mL) was immobilized on 96-well Maxisorp ELISA plates (Thermo Fisher) overnight at 4°C in coating buffer (1X PBS supplemented with 0.05% Tween-20 ...
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bioRxiv - Molecular Biology 2020Quote: ... siRNA (4): s35234 and siRNA (5): s35235 (Silencer Select, ThermoFisher Scientific, USA); EMC2 s18670 and EMC5 s41131 (Silencer Select ...
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bioRxiv - Immunology 2021Quote: ... 2.5-5 µg was loaded onto a NuPAGE 4-12% gel (ThermoFisher) and run at 100V for 2-3 h ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and passaged every 4-5 days using StemPro Accutase (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2022Quote: ... and passaged every 4-5 days with 1 mg/ml dispase (Gibco).
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bioRxiv - Cancer Biology 2022Quote: ... 5 ng/ml IL-4 (Thermo Fisher Scientific, Cat#14-8041-62) and 1 ng/ml TGF-β1 (Cell Signaling Technology ...
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Upregulated GIRK2 counteracts ethanol-induced changes in excitability & respiration in human neuronsbioRxiv - Neuroscience 2024Quote: ... After 4-5 minutes 30 µM SCH-23390 (Fisher Scientific Cat#: 092550) in ACSF was applied for 1 minute ...
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bioRxiv - Neuroscience 2024Quote: ... and passaged every 4-5 days with 1 mg/ml Dispase (Gibco). The human FUSWT line ...
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bioRxiv - Bioengineering 2023Quote: Samples were loaded with 5 μM Fluo-4 AM (ThermoFisher Scientific, Germany) in CM for 30 min in a cell culture incubator (37 °C and humidified 5% CO2) ...
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bioRxiv - Cancer Biology 2024Quote: ... and incubated overnight at 4° C with 5 mM MANT-GDP (Invitrogen). Excess nucleotide was removed using a GE FPLC using a HighPrep 26/10 column into a buffer containing 20 mM HEPES (pH 8.0) ...
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bioRxiv - Cell Biology 2024Quote: ... 3-4 or 5-6 and Phusion DNA polymerase (Thermo Fisher Scientific). The resulting PCR mix was DpnI digested and 4 μl of the mix was used for transformation of E ...
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bioRxiv - Cell Biology 2024Quote: ... 4-5×106 cells were resuspended in 100 μL of DMEM (Invitrogen) and 4 μg of plasmid DNA ...
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bioRxiv - Plant Biology 2021Quote: ... The supernatant was discarded and the pellet was resuspended in 5 mL liquid KNOP supplemented with 2% (w/v) sucrose (#S/8600/60, Fisher) and 100 μM 3’,5’-dimethoxy-4’-hydroxyacetophenone (acetosyringone) (#115540050, Acros Organics, dissolved in dimethyl sulfoxide (DMSO) (#D8418 ...
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bioRxiv - Bioengineering 2022Quote: ... 4-Chlorobenzenesulfonate Salt (DID) and 4′,6-diamidino-2-phenylindole (DAPI) were purchased from Invitrogen (Carlsbad, CA, USA). Ammonium bicarbonate ...
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bioRxiv - Microbiology 2023Quote: ... and counterstained with 4′,6-diamidin-2-phenylindole (DAPI, 4 μg mL-1, Thermo Fisher Scientific, MA, USA). From the 18 biofilms from each year ...
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bioRxiv - Bioengineering 2023Quote: ... The cells were washed 4 times with PBST and counterstained with 4′,6-diamidino-2-phenylindole (DAPI; Invitrogen) at 1:1000 dilution ...
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bioRxiv - Developmental Biology 2023Quote: ... ATs and TTs were fixed in 4% PFA and stained with 4′,6- diamidino-2-phenylindole (DAPI) (Invitrogen) to identify cell nuclei ...
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bioRxiv - Cell Biology 2020Quote: ... 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxy-d-glucose (2-NBDG) was purchased from Invitrogen (Carlsbad, CA, USA). Trypsin-EDTA solution was purchased from GIBCO BRL (Grand Island ...
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bioRxiv - Cancer Biology 2024Quote: ... Above cells were split every 4-5 days using 5-10-minute incubation with 0.05% trypsin-EDTA (Thermo Fisher) at 37 °C ...
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bioRxiv - Microbiology 2024Quote: ... centrifuged at 5 000×g for 5 min at 4°C in SpinX 0,2 µm tubes (Corning, Fisher Scientific) for cell debris and bacterial removal ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were resuspended in MACS buffer containing 2 µg/mL 4’,6-diamidino-2-phenylindole (DAPI; Life Technologies). A BD FACSAria III Cell Sorter (BD Biosciences ...
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bioRxiv - Genetics 2020Quote: ... Cells were passaged 1:2-1:6 every 2-4 days by being rinsed once with DPBS (Gibco) and dissociated using 0.5 mM EDTA (75 µl/cm2 ...
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bioRxiv - Neuroscience 2024Quote: ... Nuclear staining was performed using 4′,6′-diamidino-2-phenylindole dihydrochloride (DAPI; 2 ng ml−1; Molecular Probes). Sections were cover-slipped using ProLong Glass mounting agent (ThermoFisher) ...
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bioRxiv - Bioengineering 2024Quote: ... Cell nuclei were counter stained with 4’,6’-diamidino-2-phenylindole dihydrochloride (DAPI; 2 ng/ml; Molecular Probes) prior to mounting onto glass slides and then cover slipped with ProLong Gold (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... slices were incubated in secondary antibody (Table 2) with 4’,6-diamidino-2-phenylindolefor (DAPI, 1:1000, Invitrogen) 45 minutes at room temperature in a rocking platform ...
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bioRxiv - Cancer Biology 2022Quote: ... in 500 μL HEPES (25 mM)/PBS were mixed overnight at 4 °C with 5 μL of 4 μm aldehyde-activated latex beads (A37304, Invitrogen). The beads were blocked with 200 μL of 1% BSA for 1 h at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... Supernatant containing P1 virus was harvested ∼4 days post transfection and stored at 4°C after supplementing 5% FBS (Gibco). Expression of the Sec complex was carried out by adding 0.5 mL P1 virus to 0.7 L of Sf9 cells at density of ∼1.5 M/ml that were prepared in a 2-L baffled flask ...
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bioRxiv - Cell Biology 2023Quote: ... with NaOH) and were loaded with 5 μM Ca2+ dye Fluo-4 acetomethyl ester (Fluo-4 AM) (Invitrogen Life Technologies) for 20 minutes at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... with NaOH) and were loaded with 5 μM Ca2+ dye Fluo-4 acetomethyl ester (Fluo-4 AM) (Invitrogen Life Technologies) for 20 minutes at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... N,N-dimethyl formamide (DMF) and water and HPLC grade methyl tert-butyl ether (MTBE) were obtained from Thermo Fisher used throughout ...
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bioRxiv - Molecular Biology 2021Quote: Tert-immortalized keratinocytes (kindly provided by Jerry Shay, UT-Southwestern) were cultured in EpiLife Medium (catalogue # MEPICFPRF500, Thermo Fisher Scientific) supplemented with human keratinocyte growth supplement (catalogue #S-001-5 ...
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bioRxiv - Genetics 2024Quote: ... Wild type N/TERT-2G cell line was cultured using EpiLife medium with 60 µM calcium (Gibco™, cat. #MEPI500CA), supplemented with human keratinocyte growth supplement (HKGS ...
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bioRxiv - Microbiology 2024Quote: ... The Na+-specific ionophore monensin (Solarbo, China) and the protonophore 3,5-di-tert-butyl4-hydroxybenzaldehyde (DTHB, Thermo Fisher Scientific, USA) were used to test which pathway drives ATP synthesis ...
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bioRxiv - Bioengineering 2023Quote: ... HA-tetrazine was synthesized as described previously using 79 kDa HA with molar equivalents of 1:1:0.25 of HA-repeat units to 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMTMM) (Thermo Fisher Scientific) to tetrazine-amine (Chem-Impex).[16] 1H NMR shifts of pendant tetrazine groups at δ8.5 (2H ...
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bioRxiv - Immunology 2024Quote: ... The cells were cultured for 2 weeks and then washed and fixed in 4% paraformaldehyde (4% PFA; Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2022Quote: ... discoideum clones on SM/5 plates (2 g glucose (Fisher Scientific), 2 g Bacto Peptone (Oxoid) ...