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Citations for Thermo Fisher :
451 - 500 of 10000+ citations for 4 Keto 13 cis retinoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... following which they were stained with anti-Myc FITC (Invitrogen, 13-2511) and anti-human IgG BV421 (BD Biosciences ...
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bioRxiv - Immunology 2024Quote: ... Cocktail of phorbol 12-myristate 13-acetate (PMA) and ionomycin (Thermo Scientific) was used as positive control and cell culture medium as negative control ...
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RNA Binding of GAPDH Controls Transcript Stability and Protein Translation in Acute Myeloid LeukemiabioRxiv - Cancer Biology 2024Quote: RNA was extracted from MOLM-13 cells using TRIzol (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: Filtros Corning SFCA 0,22 µm (Fisher Scientific, cat. n° 09-754-13)
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bioRxiv - Plant Biology 2021Quote: ... After measuring initial electrical conductivity (Ci) using a conductivity meter (Cyberscan CON 11, Eutech Instrument, Thermofisher, Singapore), the leaf samples were boiled for 20 min and measured for the conductivity of lysed cells (Cmax) ...
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bioRxiv - Plant Biology 2021Quote: ... The initial conductance reading (Ci) of the incubated solution was taken using a conductivity meter (Thermo Scientific). Then the leaf samples were autoclaved at 121 °C for 20 min and shaking for another 24 hr ...
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bioRxiv - Molecular Biology 2021Quote: ... or amino acids (MEM amino acids solution; Gibco, Waltham, MA, USA).
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bioRxiv - Biochemistry 2024Quote: ... Except for lauric acid (Henkel) and 1,18-Octadecanedicarboxylic acid (Thermofisher Scientific), lipids were from Merck ...
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bioRxiv - Plant Biology 2021Quote: ... the peptides were dissolved in 4 μL of 0.2% formic acid (FA) and injected into an Easy-nLC 1200 (Thermo Fisher Scientific). Peptides were separated on a 15 cm in-house packed column (360 μm OD × 75 μm ID ...
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bioRxiv - Neuroscience 2020Quote: ... Fluo-4 AM dye was dissolved in a mixture of dimethyl sulfoxide (DMSO) and 20% pluronic acid (Invitrogen, Burlington, Ontario, Canada) to a 0.5 mM stock solution and kept frozen until used ...
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bioRxiv - Immunology 2021Quote: ... Peptide and enzyme solutions were dissolved and diluted in 25 mM 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid (HEPES, Fisher Scientific). The peptide (100 μM in a total volume of 100 μL ...
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bioRxiv - Biochemistry 2020Quote: ... 500 mM 6-aminohexanoic acid) was added to lysates right before loading samples into a NativePAGE Bis-Tris gel 4-16% (Life Technologies). The proteins were separated according to Wittig et al.32 and transferred onto a PVDF membrane using a standard Tris-glycine transfer buffer with 0.05% SDS ...
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bioRxiv - Cancer Biology 2021Quote: ... slides were incubated overnight in a humidified chamber at 4 °C in TBS-T with 1% Fatty acid free-BSA (Fisher Scientific) with anti-mouse/human antibodies ...
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bioRxiv - Immunology 2021Quote: ... PBMCs were incubated for 20 min at 37°C in PBS containing 4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-hexadecanoic acid (BODIPY FL C16; 1 μM; Thermo Fisher Scientific). To determine neutral lipid content ...
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bioRxiv - Immunology 2022Quote: ... for 20 min before incubation overnight at 4°C in PBS-BR containing any of the following mAbs or reagents: DAPI nucleic acid (Molecular Probes), phalloidin Alexa fluor 488 (Molecular Probes) ...
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bioRxiv - Cancer Biology 2022Quote: ... The stock solutions of the agonists were made in 25 mM 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid (HEPES, Fisher Scientific) and the enzyme inhibitors were reconstituted according to manufacturers’ instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were cleared by centrifugation at 21,000 x g for 10 minutes at 4°C and protein concentration was determined by Bicinchoninic acid assay (Pierce, ThermoFisher Scientific). Proteins were reduced with 5 mM dithiothreitol for 30 minutes at 55°C ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were dissolved in 10 µL of reconstitution buffer (96:4 water: acetonitrile, 1% formic acid and analyzed by LC-MS/MS using QExactive (Thermo Fisher) in the proteomics core facility at EMBL Heidelberg (https://www.embl.org/groups/proteomics/).
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bioRxiv - Cell Biology 2024Quote: ... as described above and separated using a linear 12.5 min gradient of 4-32% aqueous acetonitrile versus 0.1% formic acid on a nanoflow chromatography system (Ultimate nanoRSLC, Thermo Fisher Scientific) using a reversed phase-C18 column (PepSep Fifteen ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Lot 134874), and 4-(2-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, purity ≥ 99.%, CAT #BP310-1, Lot 052975) were from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were dissolved in 10 µL of reconstitution buffer (96:4 water: acetonitrile, 1% formic acid and analyzed by LC-MS/MS using QExactive (Thermo Fisher) in the proteomics core facility at EMBL Heidelberg (https://www.embl.org/groups/proteomics/) ...
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bioRxiv - Cell Biology 2023Quote: Peptides were dissolved in 4 μL 0.2% (v/v) formic acid (FA) and injected into an Easy-nLC 1200 (Thermo Fisher Scientific). Peptides were separated on a 15-cm in-house packed column (360 μm OD × 75 μm ID ...
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bioRxiv - Immunology 2023Quote: ... The WIV was then dialyzed against 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES)-buffered saline (Thermo Fisher Scientific, Bleiswijk, the Netherlands) at 4°C overnight to remove β-propiolactone ...
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bioRxiv - Immunology 2023Quote: ... supplemented to a final concentration of 0.1% Pluronic Acid F-68 and 20% 4 mM L-glutamine using Expifectamine transfection reagent (Thermo Fisher Scientific) cultured for 4-7 days at 8% CO2 saturation and 37°C with shaking ...
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bioRxiv - Immunology 2023Quote: ... supplemented to a final concentration of 0.1% Pluronic Acid F-68 and 20% 4 mM L-glutamine using Expifectamine transfection reagent (Thermo Fisher Scientific). Briefly ...
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bioRxiv - Microbiology 2024Quote: ... 20% volume of 1M 2-morpholin-4-ylethanesulfonic acid (MES) pH 5 and Immobilized Protein G resin (Thermo Scientific Prod#20397) were added to supernatant and sample was incubated overnight at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... Total protein concentration was quantified by Bicinchoninic acid (BCA) assay and 15 μg separated on 4-12% Bolt Bis-Tris polyacrylamide gels (Thermo Scientific), transferred to nitrocellulose (iBlot ...
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bioRxiv - Bioengineering 2024Quote: ... Agarose (IB70040), alpha-Cyano-4-hydroxycinnamic acid (J67635.EXK), and activated alumina (Brockman I, basic) (6002042) were purchased from Thermo Scientific. Novozym 435 was received as a gift from Novozymes ...
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bioRxiv - Neuroscience 2024Quote: ... was dissolved in 4 µl of dimethyl sulfoxide (DMSO) containing 20% Pluronic F-127 acid (Invitrogen by Thermo Fisher Scientific, USA). The dye solution was diluted in an extracellular buffer consisting of 150 mM NaCl ...
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bioRxiv - Neuroscience 2024Quote: ... was dissolved in 4 µl of dimethyl sulfoxide (DMSO) containing 20% Pluronic F-127 acid (Invitrogen by Thermo Fisher Scientific, USA). The dye solution was diluted in an extracellular buffer consisting of 150 mM NaCl ...
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bioRxiv - Cancer Biology 2024Quote: ... further supplemented with 10% (v/v) heat-inactivated fetal bovine serum (FBS) and 25 mM 4-(2-hydroxyethyl)-1- piperazineethanesulfonic acid (HEPEs) buffer (Gibco, US), 2.5g/l Glucose (Merck ...
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bioRxiv - Cancer Biology 2024Quote: ... The lysates were then clarified by centrifugation at 15,000 × g for 30 minutes at 4 °C and quantified using a Bicinchoninic Acid assay (Pierce BCA, Thermo Fisher Scientific), following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... From day 13 onwards the cells were cultured in E8 life (Thermo Fisher) until day 20 ...
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bioRxiv - Neuroscience 2022Quote: ... Astrocyte cultures were transfected at day in vitro 13 using lipofectamine 2000 (Invitrogen) transfection following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... filtered through a 0.45 μm filter (Fisher Scientific, catalog number 13-1001-07), and used immediately or stored at −80°C ...
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The structure of insulin granule core determines secretory capacity being reduced in type-2 diabetesbioRxiv - Physiology 2022Quote: ... INS-1 832/13 cells were cultured in RPMI-1640 (Gibco BRL, NY) with 11.2 mmol/l glucose and 2 mmol/l l-glutamine ...
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bioRxiv - Cancer Biology 2022Quote: ... or Rat anti-GFAP monoclonal antibody (Invitrogen, #13-0300, 1:100 v/v) overnight at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... E-cadherin (Cell Signaling 3195, 1:200 or Invitrogen 13-1900, 1:200), Lef1 (Cell Signaling 2230 ...
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bioRxiv - Biophysics 2020Quote: 13) Alexa Fluor 647 NHS Ester (Succinimidyl Ester) (Thermo Fisher Scientific, Cat# A20106) ! CAUTION keep in dark at -20°C.
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bioRxiv - Molecular Biology 2020Quote: ... cells were treated with Phorbol 12-Myristate 13-Acétate (Fisher Scientific, Cat#10061403), Human EGF (Euromedex ...
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bioRxiv - Cell Biology 2020Quote: ... U2OS-CASP2-/- cells were seeded onto 13 mm glass coverslips (Thermo Fisher Scientific) and incubated overnight at 37 °C in a 10 % CO2 incubator ...
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bioRxiv - Microbiology 2021Quote: Biofilms were developed on plastic coverslips (diameter = 13 mm, thickness = 0.2 mm) (Nunc Thermanox TM ...
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bioRxiv - Developmental Biology 2021Quote: ... and rat anti-E-cadherin (1:1000, Thermo Fisher, Cat. No. 13-1900). Sections were washed in PBS with 0.1% Tween-20 four times for 5 minutes at room temperature and incubated in secondary antibodies and DAPI (1:500 dilution of 1 mg/ml stock ...
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bioRxiv - Genomics 2021Quote: ... the DNA quality was first confirmed by Nanodrop (ThermoFisher, catalog # 13-400-518) with 260/280 ratio at 1.95-2.0 (in 10 mM Tris·Cl ...
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bioRxiv - Immunology 2022Quote: ... and IL-13 Alexa Fluor 488 (ThermoFisher, cat. 53-7133-82, clone eBio13A). Finally ...
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bioRxiv - Neuroscience 2022Quote: ... Neurons were transfected at days in vitro (DIV) 13 using Lipofectamine 2000 (Invitrogen). The next day ...
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bioRxiv - Cell Biology 2022Quote: ... 13-0193-86) followed by staining with streptavidin-PE-Texas Red (Invitrogen, SA1017), rat anti-mouse cKit APC-Cy7 (eBioscience ...
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bioRxiv - Microbiology 2022Quote: ... A549 +ACE2 +TMPRSS2 cells were grown on 13-mm Thermanox (plastic) coverslips (Nunc) in the wells of a 24-well plate ...
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bioRxiv - Cell Biology 2023Quote: ... cells were seeded onto 13-mm-diameter round coverslips (cat. # 174950, Thermo Fisher,) pre-coated with 10 μg/mL laminin (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: PA-14-13 Patient-Derived Organoids were cultured in Advanced DMEM/F12 (Gibco), supplemented with 5% fetal bovine serum (FBS ...