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Citations for Thermo Fisher :
451 - 500 of 10000+ citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... slices were incubated in secondary antibody (Table 2) with 4’,6-diamidino-2-phenylindolefor (DAPI, 1:1000, Invitrogen) 45 minutes at room temperature in a rocking platform ...
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bioRxiv - Neuroscience 2022Quote: ... the medium was replaced every 2-3 days and cells passaged 1:2 or 1:3 weekly with 0.25% Trypsin/EDTA (Thermo Fisher Scientific, #25200-056) pre-warmed at 37°C.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Cells were grown exponentially in an incubator at 37°C under 5% CO2 and subcultured at ratios of 1:2-1:10 every 2 to 4 days using 0.05% trypsin-EDTA (Thermo Fisher Scientific, Cat# 25300120).
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bioRxiv - Neuroscience 2024Quote: ... The homogenate was diluted 1:1 with PBS with 2 x Protease/phosphatase inhibitors (2 tablets Thermo Scientific Pierce # A32961 per 10ml PBS). A portion of the resulting 10 % (w/v ...
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bioRxiv - Developmental Biology 2024Quote: ... lyz:DsRed2nz50double transgenic fish (2 males and 2 females) were removed with forceps and placed in dissociation media (1:1 DMEM:Liberase) (Gibco A1896701/ Sigma-Aldrich 05401119001). The tissue was slowly pipetted up and down at room temperature for 45 minutes with a 1000 µL pipette set to 500 µL to ensure gentle dissociation ...
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bioRxiv - Microbiology 2020Quote: ... Myoblat and myotubes viability was determined by 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT; Life Technologies) metabolization.
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bioRxiv - Cancer Biology 2020Quote: ... then assayed in a standard MTT assay: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (ThermoFisher, Waltham, MA) [39] ...
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bioRxiv - Immunology 2020Quote: Cell viability was estimated by a quantitative colorimetric assay described for human granulocytes which were based on metabolic reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Invitrogen) into coloured product formazan (Oez ...
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bioRxiv - Cell Biology 2023Quote: ... HSPCs were incubated with 20µM NBD C6-Ceramide (6-((N-(7-Nitrobenz-2-Oxa-1,3-Diazol-4-yl)amino)hexanoyl)Sphingosine) (Invitrogen) for 30 minutes at 4°C for Golgi staining or Cytopainter (Abcam ...
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bioRxiv - Cancer Biology 2024Quote: ... or using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reagent (Fisher Scientific, AC158992500, Waltham, MA, USA) at 570 nm ...
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bioRxiv - Cancer Biology 2023Quote: Cell proliferation was assayed by reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; Invitrogen, M6494). MTT was freshly dissolved into PBS at a stock concentration of 12 mM and diluted into phenol red-free DMEM with 10% FBS for a final MTT concentration of 2 mM ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Cell viability was measured with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Thermo Fisher Scientific, M6394). After cell exposure to AG490 and MeHg at specified times and concentrations ...
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bioRxiv - Cell Biology 2024Quote: ... and IPF fibroblasts underwent the 3-(4,5-Dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay (ThermoFisher Scientific, M6494) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... 100 μM 2-mercaptoethanol (2-ME, Gibco) and 1 × Antibiotic-Antimycotic (Gibco ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 mM of 4-(2-hydroxyethyl)-1-piperazine-1-ethanesulfonic acid (HEPES) (Gibco) and 1 ng/mL of human bFGF (Sigma-Aldrich) ...
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bioRxiv - Immunology 2022Quote: ... 1 - 2 μg ml-1 poly(dA:dT) (InvivoGen) (transfected with Lipofectamine 2000, Invitrogen), 100 µM imiquimod (R837 ...
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bioRxiv - Neuroscience 2022Quote: ... IL-6 (1:4-1:2 dilution, #KMC0061; ThermoFisher Scientific, Waltham, MA, USA), and NfL (1:4 dilution ...
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bioRxiv - Immunology 2024Quote: ... at a 1:1 ratio and recombinant human IL-2 (Peprotech, ThermoFisher Scientific), and expanded in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Neuroscience 2022Quote: ... 1:2000) and 4′,6-diamidino-2-phenylindole (DAPI, 1:1,000, Life Technologies). Following several washes ...
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bioRxiv - Neuroscience 2023Quote: ... containing 2% B27 and 1% L-glutamine and 1% Pen-Strep (Life Technologies). Cultured neurons were fed with 250 μl NB+ media on days in vitro (DIV ...
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bioRxiv - Evolutionary Biology 2023Quote: ... supplemented with 1/2 x N2 and 1 x B27 (Thermo Fisher Scientific), 1% Penicillin/Streptomycin (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer (1 M) (Invitrogen, cat.#15630080), CellMask membrane dye (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... metaphase II-arrested eggs were microinjected with 2-3 picolitres of Rec8 antiserum (13) (1:2 dilution) and Alexa Fluor 488 Dextran 10,000 MW (Molecular Probes, D22910; 1:40 dilution) in 0.05% (v/v ...
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bioRxiv - Bioengineering 2024Quote: ... PCs and ACs were stained with neural/glial antigen 2 (NG-2) antibody (eBioscience, 14-6504-80, 1:100) and glial fibrillary acidic protein (GFAP) antibody (Invitrogen, PA1-10019, 1:1000), respectively ...
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bioRxiv - Genetics 2024Quote: ... 2 µL of a 1:100 dilution of ERCC ExFold RNA Spike-In Mix 1 or Mix 2 (Thermo Fisher Scientific, #44-567-39) were added to 1 µg of total RNA ...
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bioRxiv - Physiology 2020Quote: ... The membrane was probed with primary antibodies for α-Tubulin (ThermoFisher 322588, clone B-5-1-2), acetylated (Sigma T7451 ...
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bioRxiv - Developmental Biology 2024Quote: ... was prepared and added to 426.5 μL 2:1 (vol:vol) 0.1x Tris (Fisher Scientific, cat.# BP152-5)-ethylenediaminetetraacetic acid (EDTA ...
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bioRxiv - Neuroscience 2024Quote: ... Nuclei were stained for 5 minutes with 1 µM 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI; Invitrogen), coverslips were mounted on glass slides with mounting medium (Dako) ...
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bioRxiv - Bioengineering 2024Quote: ... 2 µL mL-1 egg lecithin (5 mM in EtOH) and 1x chemical defined lipid concentrate (Gibco). After incubation for 2 days ...
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bioRxiv - Biochemistry 2020Quote: ... 1.2M sorbitol buffer (pH 7.5) and permeabilized with 1% Triton X-100 stained with 1 μg/ml DAPI (4’, 6-diamidino-2-phenylindole; Molecular Probes).
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bioRxiv - Synthetic Biology 2020Quote: ... Cells were subcultured at a 1:5 to 1:10 ratio every 2–3 d using Trypsin-EDTA (Gibco #25300-054). The HEK293FT cell line tested negative for mycoplasma with the MycoAlert Mycoplasma Detection Kit (Lonza #LT07-318).
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bioRxiv - Cell Biology 2022Quote: ... 1.2M sorbitol buffer (pH 7.5) and permeabilized with 1% Triton X-100 stained with 1 μg/ml DAPI (4’, 6-diamidino-2-phenylindole; Molecular Probes). Cells were imaged using a DeltaVision Ultra microscope with a 60X objective (NA = 1.42) ...
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bioRxiv - Genomics 2023Quote: ... Cells were blocked for 1-2 hrs using 2% (v/v) Bovine Serum Albumin (BSA, Thermo Scientific, Cat # 37525) + 5% FBS in HBSS ...
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bioRxiv - Molecular Biology 2024Quote: Vero E6 cells growing in P24 wells were infected with SARS-CoV-2 (Wuhan-1) at a moi = 2 pfu/cell in 0.5 mL OPTIMEM (Gibco) under biosafety level 3 conditions ...
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bioRxiv - Immunology 2024Quote: ... the inoculum was replaced with 150 μL of overlay medium (1:1 mix of Leibovitz’s L-15 medium supplemented with 0.1% pen/strep, 2% TPB, 1× NEAA, 2× Antibiotic-Antimycotic [Life Technologies] ...
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bioRxiv - Genomics 2020Quote: ... and 1 μL of 10 μM de-oxy-nucleoside triphosphates (Invitrogen, Carlsbad, CA, USA) were treated for 5 min at 65°C and added to 10 μL reverse transcription master mix containing 200 U/μL M-MLV RT enzyme (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by 4’,6diamidino-2-phenylindole (DAPI, 1:300, Invitrogen) staining for 10 min ...
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bioRxiv - Neuroscience 2021Quote: ... 2°: goat anti-mouse AF488 (1:500) (Invitrogen, A-11001), and donkey anti-rabbit Cy5 (1:200 ...
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bioRxiv - Neuroscience 2021Quote: ... and ethidium homodimer-1 (2 mM in DMSO, L3224, ThermoFisher) were added directly to the cell medium to a final concentration of 1 μM each ...
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bioRxiv - Neuroscience 2021Quote: ... stained with Neurotrace for 2 hours (1:500, N21479; Invitrogen), rinsed twice with 2x SSCT and mounted on a slide with Fluoromount-G ...
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bioRxiv - Genomics 2020Quote: ... 4’,6-diamidino-2-phenylindole (DAPI) (1:1000) (Life Technologies) was added to visualize cell nuclei ...
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bioRxiv - Molecular Biology 2020Quote: ... and HA tag (2-2.2.14; ThermoFisher Scientific 26183, 1:10,000), used with goat anti-mouse IgG AlexaFluor®568 (ThermoFisher Scientific A-11004 ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were loaded with 1 μM fura-2 AM (Invitrogen) for 40 min before the measurements at 37°C ...
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bioRxiv - Systems Biology 2021Quote: ... 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, Gibco), and 1× MEM non-essential amino acids solution (Gibco) ...
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bioRxiv - Bioengineering 2021Quote: ... 1-2 drops of CytoSeal mounting media (Thermofisher, 8312-4) was placed on each slide ...
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bioRxiv - Bioengineering 2021Quote: ... 1 ng/mL human FGF-2 (ThermoFisher #68-8785-63), and 1% Primocin (Invivogen #ant-pm-1 ...
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bioRxiv - Neuroscience 2020Quote: ... 1% L-glutamine and 2% penicillin/streptomycin (Invitrogen, Paisley, UK). All cells were incubated at 5% CO2 in a humidity-controlled environment (37°C ...
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bioRxiv - Microbiology 2020Quote: ... transfection reagent (1:2 µg:µL ratio) in Opti-MEM (Gibco), serial diluted in 4-fold increments (1µg to 0.016µg RNA final) ...
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bioRxiv - Neuroscience 2020Quote: ... 2) streptavidin conjugated with Alexa Fluor488 (1:1000) (Thermofisher Science), or 3 ...
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bioRxiv - Neuroscience 2022Quote: ... 1 μg GFP plasmid and 2 μl Lipofectamine 2000 (Thermofisher) with DNA:reagent ratio 1:2 were added into 25 μl of DMEM media in separate with DNA:reagent ratio 1:2 were added into 25 μl of DMEM media in separatetubes and incubated for 5 min at room temperature ...