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Citations for Thermo Fisher :
451 - 500 of 588 citations for "PVDF Membrane" since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2020Quote: ... Western Blot: Samples were processed as described for SDS PAGE and transferred to PVDF membrane with iBlot2 (ThermoFisher). After transfer ...
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Sapogenin based self-assembly structures activating a non-apoptotic cell death via multiple pathwaysbioRxiv - Pharmacology and Toxicology 2021Quote: ... proteins were separated by SDS-PAGE electrophoresis and transferred to PVDF membranes (EMD Millipore, Thermo Fisher Scientific, US).
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bioRxiv - Neuroscience 2021Quote: ... Proteins were transferred from the gel to PVDF membranes in an iBlot2 device (Thermo Fisher, cat no. IB24001). Depending on the protein ...
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bioRxiv - Cell Biology 2022Quote: ... The protein was transferred from the gel onto PVDF membrane using an iBlot gel transfer system (ThermoFisher Scientific). Antibody incubations were carried out in 5% nonfat milk in TBS buffer containing 0.2% Tween-20 ...
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bioRxiv - Genomics 2022Quote: PVDF membranes containing transferred proteins were incubated in blocking buffer (1 x TBS-T (Thermo Fisher Scientific, 28360), 5% Blotting-Grade Blocker (BioRad ...
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bioRxiv - Plant Biology 2022Quote: ... Western blots using PVDF membranes were prepared and probed using HRP-conjugated antibodies in 0.2% I-Block (Invitrogen).
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bioRxiv - Neuroscience 2023Quote: ... A wet blotting method was used to transfer the proteins onto a PVDF membrane (Life Technologies, 0.45 µm), the membrane was then incubated with 8% acetic acid for 15min and washed with methanol and water before being blocked with 5% BSA in 20 mM Tris ...
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bioRxiv - Cancer Biology 2023Quote: ... and blotted onto a PVDF membrane using an iBlot transfer system and transfer stacks (Invitrogen, Thermo Scientific IB401001). Proteins were detected using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Thermo Scientific 34580) ...
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bioRxiv - Plant Biology 2023Quote: ... Western blots using PVDF membranes were prepared and probed using HRP-conjugated antibodies in 0.2% I-Block (Invitrogen). Detection was achieved using the Clarity™ MAX Western ECL Substrate (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... The gel was transferred to PVDF membrane using an iBLOT dry transfer system (Thermo Fisher Sci, Waltham, MA). The blots were cut into strips to give B6 and B6 and B6 ...
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bioRxiv - Cancer Biology 2023Quote: ... and blotted onto a PVDF membrane using an iBlot transfer system and transfer stacks (Invitrogen, Thermo Scientific IB401001). Proteins were detected using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Thermo Scientific 34580) ...
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bioRxiv - Cancer Biology 2023Quote: ... and blotted onto a PVDF membrane using an iBlot transfer system and transfer stacks (Invitrogen, Thermo Scientific IB401001). Proteins were detected using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Thermo Scientific 34580) ...
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bioRxiv - Cancer Biology 2023Quote: ... and blotted onto a PVDF membrane using an iBlot transfer system and transfer stacks (Invitrogen, Thermo Scientific IB401001). Proteins were detected using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Thermo Scientific 34580) ...
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bioRxiv - Cell Biology 2023Quote: ... total protein loading on the PVDF membrane was stained with No-StainTM Protein Labeling reagent (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... The gel was then removed and placed into a sandwich with a PVDF membrane (IPVH00010, Thermo Fisher Scientific), and sponge pads in a gel blotting cassette (A25977 ...
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bioRxiv - Physiology 2023Quote: ... Transfer was performed on the iBlot system from lnvitrogen with PVDF membranes and Tropix I-Block (Life Technologies) as a blocking agent ...
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bioRxiv - Cancer Biology 2023Quote: ... for 45 minutes and 80 mAmps and then transferred to a 45uM PVDF membrane (Thermo Scientific REF 88518). The transfer was run using the NuPage transfer buffer for 90 minutes at 20 volts ...
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bioRxiv - Cancer Biology 2021Quote: ... Equivalent amounts of protein were resolved on precast polyacrylamide denaturing gels and were then transferred onto 0.2 μm PVDF membranes using either dry transfer with an iBLOT2 system (ThermoFisher) or conventional wet transfer ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by the transferring of resolved proteins onto PVDF membranes via an iBlot 2 Dry Blotting System (ThermoFisher Scientific). Following blocking for 60 min with 1% BSA in TBST (10 mM Tris-HCl (pH7.4) ...
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bioRxiv - Cell Biology 2022Quote: ... or 10-20% Tris-glycine gel (for LC3) and then electro-transferred to a 0.45 μm (or 0.2 μm) PVDF membrane (Thermo Scientific). After blocking with 5% milk ...
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bioRxiv - Plant Biology 2020Quote: ... proteins were transferred to PVDF membranes (Roth) according to the manufacturer’s instructions (iBlot dry blotting system, Thermo Fisher Scientific); however ...
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bioRxiv - Cell Biology 2022Quote: ... transferred to PVDF membranes (Amersham Hybond) and probed with the following antibodies: mouse anti-V5 (1:1,000) (R960-25, Invitrogen), mouse anti-TRP (1:300 ...
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bioRxiv - Biochemistry 2022Quote: ... the separated proteins were transferred onto polyvinylidene difluoride (PVDF) membrane using iBlot™ 2 Dry Blotting System (ThermoFisher Scientific). The blot was blocked with 5% skim milk in 1xPBS and probed with the anti-mono-ADP-ribose binding reagent/antibody MABE1076 (α-MAR) ...
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bioRxiv - Neuroscience 2021Quote: ... The proteins were blotted onto a polyvinylidene fluoride (PVDF) membrane using the iBlot Dry Blotting System (Thermo Fisher Scientific). Membranes were incubated with anti-Thioredoxin1 rabbit antibody (CST#2298 ...
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bioRxiv - Neuroscience 2022Quote: ... The transfer conditions used were a 0.45-μm polyvinylidene fluoride (PVDF) membrane transferred in 5% Transfer Buffer (Invitrogen, NP00061) at 20 mA for 840 min ...
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bioRxiv - Neuroscience 2020Quote: ... and subsequently transferred to Polyvinylidene difluoride (PVDF) membranes using the iBlot 2 Dry Blotting System (Invitrogen, Carlbad, MA, USA). Unspecific antibody binding was prevented by incubating the blots in blocking solution containing 5% non-fat milk or 5% BSA (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2019Quote: ... electrophoresed with MES running buffer and then transferred onto 0.45 µm PVDF membrane by electroblot using a Mini Blot module (Invitrogen). 4-HNE protein adducts were detected by an anti 4-HNE protein adduct antibody (1:2000 ...
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bioRxiv - Microbiology 2021Quote: ... proteins separated by 12%non-reducing SDS-PAGE and transferred onto a polyvinylidene difluoride (PVDF) membrane (Thermo Fisher Scientific). The membrane was blocked with TBST buffer containing 3% milk powder and 1% BSA ...
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bioRxiv - Biochemistry 2020Quote: ... the separated proteins were transferred onto polyvinylidene difluoride (PVDF) membrane using iBlot™ 2 Dry Blotting System (ThermoFisher Scientific). The blot was blocked with 5% skim milk in PBS containing 0.05% Tween-20 and probed with anti-mono or poly ADP-ribose binding reagents/antibodies MABE1076 (α-MAR) ...
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bioRxiv - Molecular Biology 2021Quote: ... A total of 2 μg of protein per lane was loaded on a 4-12% graded Tris-glycine SDS-polyacrylamide gel and proteins were transferred to a 0.2μm PVDF membrane (Life Technologies). Membranes were blocked in 5% milk in PBS with 0.05% Tween-20 for one hour at room temperature with rocking ...
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bioRxiv - Microbiology 2022Quote: ... and the separated proteins were transferred by dry blotting onto PVDF membranes according to the manufacturer’s instructions (Life Technologies). Similarly ...
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bioRxiv - Cell Biology 2023Quote: ... the gel was transferred to an iBlot PVDF membrane using the iBlot™ 2 Gel Transfer Device (ThermoFisher Scientific) under standard conditions ...
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bioRxiv - Synthetic Biology 2023Quote: ... proteins were transferred onto a 0.2 μM PVDF membrane (Cytiva) using Power Blotter 1-Step™ Transfer Buffer (Invitrogen) for 10 minutes at 1.3 A ...
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bioRxiv - Plant Biology 2023Quote: ... Separated proteins were transferred to a PVDF membrane and probed with monoclonal mouse anti-Myc (1:3,000; R950-25, Thermofisher), polyclonal rabbit anti-GFP (1:3,000 ...
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bioRxiv - Plant Biology 2023Quote: ... Separated proteins were transferred to a PVDF membrane and probed with monoclonal mouse anti-Myc (1:3,000; R950-25, Thermofisher), polyclonal rabbit anti-GFP (1:3,000 ...
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bioRxiv - Microbiology 2024Quote: ... SDS-PAGE gel was transferred onto PVDF membrane using the iBlot™ Gel Transfer Device and Stacks (Thermo Fisher). Membranes were blocked for 1h at room temperature in TBS 1x/0.1% Tween-20/5% milk ...
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bioRxiv - Microbiology 2024Quote: ... the separated proteins were transferred onto polyvinylidene difluoride (PVDF) membrane using iBlot™ 2 Dry Blotting System (ThermoFisher Scientific). The blot was blocked with 5% skim milk in PBS containing 0.05% Tween-20 and probed with anti-mono ADP-ribose binding reagent MABE1076 (α-MAR ...
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bioRxiv - Biophysics 2024Quote: ... SDS-PAGE gel was transferred to PVDF membrane at 30 V for 45 min in NuPAGE Transfer Buffer (Invitrogen) and blocked with 4% BSA in TTBS [100 mM Tris/HCl pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... Denatured protein samples were then resolved on a 10% polyacrylamide gel and transferred to a PVDF membrane (Thermo Scientific, 88518). Blots were probed as previously described 37 ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were migrated on a SDS-PAGE gel (12% acrylamide) and transferred to Amersham Hybond P 0.2 PVDF membranes using a Pierce G2 Fast Blotter (Thermo Scientific). Membranes were blocked in 5% w/v milk or BSA ...
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bioRxiv - Genetics 2019Quote: ... the lysate fraction was run on a NuPAGE 4-12% Bis-Tris gels (Novex) and transferred to PVDF membranes in an iBlot (Invitrogen). Membranes were blocked with 5% milk powder in TBS-T and incubated overnight at 4°C with primary antibodies ...
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bioRxiv - Biochemistry 2020Quote: ... After transfer and blocking of polyvinylidene fluoride (PVDF) membranes (Immobilon) in 5% BSA TBS Tween-20 buffer (Thermo Fisher Scientific), primary antibody directed against FADS2 (PA5-87765 ...
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bioRxiv - Systems Biology 2020Quote: ... Protein lysates were separated by size on a 12% sodium dodecyl sulfatepolyacrylamid (SDS) gel and blotted onto a 0.2 µm polyvinylidene difluoride (PVDF) membrane (Thermo Scientific) by semi-dry blotting (BioRad) ...
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bioRxiv - Molecular Biology 2021Quote: ... The proteins were then transferred to polyvinylidene difluoride (PVDF) membranes (Immobilion) and the membranes probed with primary antibodies (anti-V5 epitope (Invitrogen), anti-HA epitope (Abcam) ...
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bioRxiv - Cancer Biology 2020Quote: ... The proteins were transferred onto a PVDF membrane and the membranes were probed overnight at 4 °C with the appropriate primary antibodies recognizing SLITRK6 (Invitrogen) or β-Actin (ACTB ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were separated by SDS-PAGE and transferred on a PVDF membrane from pre-casted gels (Invitrogen, Carlsbad, CA, USA). Primary antibodies were diluted in 5% non-fat milk ...
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bioRxiv - Immunology 2022Quote: ... Proteins were separated by size on a 12% sodium dodecyl sulfate polyacrylamide gel and blotted onto a 0.2 µm PVDF membrane (Thermo Scientific) by semi-dry blotting (BioRad) ...
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bioRxiv - Neuroscience 2020Quote: ... was separated on SDS-polyacrylamide gels (15% for PLP/DM20 and MBP) and blotted onto PVDF membranes (Hybond; Amersham) using the XCell II Blot Module (Invitrogen). Primary antibodies were incubated overnight at 4 °C in 5% milk in TBS with 0.1% Tween 20 ...
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bioRxiv - Immunology 2020Quote: ... Samples containing 30 µg/µl of cell lysate proteins per lane were resolved on SDS-PAGE (10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis) along with pre-stained protein ladder and transferred onto PVDF membranes (Invitrogen). The transferred membranes were blocked for 2 hours using 5% BSA in TBST (25 mm Tris-HCl ...
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bioRxiv - Microbiology 2020Quote: ... was separated on 4-12% Bis-tris SDS polyacrylamide gels (reducing) and then subjected to dry blot transfer onto PVDF membranes according to the manufacturer’s instructions (Life Technologies). The PVDF membrane was imaged using an Odyssey CLX system (Li-Cor Biosciences) ...