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Citations for Proteintech :
501 - 550 of 639 citations for Primary Human Aortic Smooth Muscle Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... slides were incubated in Permeabilization/blocking buffer (0.3% Triton-X 100 in PBS [PBT] plus 5% normal donkey serum) for 20 min then primary antibodies (Pax8,1:50,10336-1-AP, Proteintech; AcTUB,1:500, T7451, Sigma) diluted in Permeabilization/block buffer were added and incubated at 4°C overnight ...
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bioRxiv - Cell Biology 2022Quote: ... or using Dynabeads-protein G (Thermo) magnetic beads with primary antibodies (anti-GFP Genetex No#GTX113717; anti-Sec5 Proteintech No#12751-1-AP) cross-linked using dimethyl pimelimidate (Life Technologies).
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bioRxiv - Cancer Biology 2022Quote: ... Tissue slides were blocked with 1.5% normal goat serum and incubated with primary antibodies against Ki-67 (1:200 dilution, CST, 9129S) and BUD31 (1:250 dilution, Proteintech, 11798-1-AP) overnight at 4°C ...
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bioRxiv - Biochemistry 2020Quote: Primary antibody incubations were performed for 18 h at 4 °C with the following antibodies: HIF-1α (Proteintech #20960-1-AP, 1/1000), and HIF-2α (Bethyl laboratories #A700-003 ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were probed overnight at 4°C with the following primary antibodies (in 5% BSA in TBS-T): rabbit anti-katanin (1:500, Proteintech, 17560-1-AP), mouse anti-spastin (1:500 ...
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bioRxiv - Bioengineering 2021Quote: ... membranes were probed with primary antibodies overnight at 4 °C at dilutions recommended by the suppliers as follows: anti-Alix (Proteintech, 12422-1-AP), anti-TSG101 (Proteintech ...
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bioRxiv - Cancer Biology 2022Quote: ... with 5% nonfat dry milk for 1 hour at room temperature and separately incubated with primary antibodies to DYNC1H1 (Proteintech, 12345-1-AP) at 1:500 and GAPDH (Thermo Fisher ...
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bioRxiv - Bioengineering 2022Quote: ... for 1 h at RT and then overnight at 4°C in primary antibody diluted in blocking buffer: anti-QSOX1 (1:1000, ProteinTech, 12713-1-AP), anti-HIF1α (1:500 ...
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bioRxiv - Microbiology 2022Quote: ... for 30 minutes at RT and then probed with primary antibodies against ACE2 (1:3000, Abcam, ab15348; 1:3000, Proteintech, 21115-1-AP), TMPRSS2 (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... samples were incubated only for 1h at room temperature with the primary antibody (rabbit anti-Arl13b, 1:1000, ProteinTech, 13967-1-AP, RRID:AB_2121979). After being washed 3 times 5 min with PBS at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... The samples were eluted with LDS sample buffer for Western blot analysis and processed as described in the Western blotting section with primary antibodies for FLAG (R&D MAB8529) and HTRA1 (ProteinTech 55011-1-AP).
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bioRxiv - Neuroscience 2023Quote: ... Cells were incubated in PBS with 3% donkey serum and the following primary antibodies overnight at 4 °C: rabbit anti-Perilipin 2 (1:200; Proteintech 15294-1-AP), rabbit anti-ACSL1 (1:100 ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were blocked with 5% BSA in TBS containing 0.1% Tween 20 (TBST) and incubated subsequently with primary antibodies including phospho-AKT (Thr308 from Signalway Antibody, College Park, MD and Ser473 from Proteintech, Rosemont, IL), AKT (Proteintech ...
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bioRxiv - Biophysics 2023Quote: ... Then retinas were incubated for 1h in 10% normal donkey serum (NDS) and subsequently in primary antibody solution (rabbit-GFP (Proteintech, 50430-2-AP), 1:666 in PBS with 3% NDS and 0.5% Triton-X ...
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bioRxiv - Cancer Biology 2024Quote: ... resuspended in HBSS containing 0.5% BSA and incubated for 30 min at 4 °C with 10 μg/ml anti-N-cad primary antibody (Proteintech, 22018-1-AP). Cells were washed twice in HBSS/0.5% BSA and incubated with Alexa Fluor 647-conjugated donkey anti-rabbit IgG (H+L ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were blocked for 1 h in 4 % milk/TBST and then incubated with the following primary antibodies (1:1000 dilution) at 4℃ overnight: mCherry (rabbit polyclonal, Proteintech, 26765-1-AP); GFP (mouse monoclonal ...
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bioRxiv - Biophysics 2021Quote: ... the samples were incubated with the primary antibody (monoclonal mouse anti-Caspase-3, 1:100, Santa Cruz, sc-56053; monoclonal mouse anti-MLKL, 1:100, Proteintech, 66675-1-Ig) diluted in blocking solution over night at 4 °C ...
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bioRxiv - Physiology 2020Quote: ... The following primary antibodies and dilutions were used and incubated in blocking buffer overnight at 4 °C with gentle rocking: BAG3 (Proteintech, 10599-1AP, 1:5,000), BAG3 (Santa Cruz Biotechnology ...
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bioRxiv - Molecular Biology 2022Quote: ... The following primary antibodies were diluted in 0.2% BSA and incubated overnight at 4 °C: anti-WFS1 (Proteintech, Cat# 1158-1-AP, 1:100), anti-Iba1 (Novus ...
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bioRxiv - Genetics 2022Quote: ... Blots were probed overnight at 4°C with the following primary antibodies: rabbit anti-vimentin at 1:2000 (Proteintech Group Inc, 10366-1-AP), rabbit anti-collagen I at 1:2000 (Proteintech Group Inc ...
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bioRxiv - Genomics 2021Quote: ... Primary antibodies used for immunofluorescence in this study were as follows: rabbit anti-PSAP antibody (1:50 dilution; Proteintech; Cat. No. 10801-1-AP), mouse anti-LAMP2 antibody (1:100 dilution ...
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bioRxiv - Cell Biology 2022Quote: The following primary antibodies were used for immunofluorescence (IF) and western blotting (WB): rabbit α-VAMP2 (1:200 IF & 1:150 WB, Proteintech, 10135-1-AP), rabbit α-VAMP3 (1:200 IF & 1:1000 WB ...
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bioRxiv - Neuroscience 2022Quote: ... the following primary antibodies were used according to the general protocol: pan-specific rabbit anti-TDP43 (1:1000; ProteinTech #10782-2-AP; RRID #AB_615042), goat anti-CXCL10 (1:150 ...
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bioRxiv - Cell Biology 2023Quote: ... The following primary antibodies were applied in the indicated dilutions and incubated overnight at 4 °C : GADD34 (Proteintech, #10449-I-AP; 1:1,000; milk), eIF2α (Cell Signaling ...
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bioRxiv - Genetics 2024Quote: ... They were incubated with primary antibodies (either anti-Nkx6.1 (Developmental studies hybridoma bank; F55A12-concentrate: 1:200) and Arl13b (Proteintech, 17711-1-AP, 1:200) in blocking buffer) ...
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bioRxiv - Systems Biology 2023Quote: ... Membranes were then washed 3 x 10 min in TBS-T and incubated overnight in primary antibodies against Pfkfb3 (Proteintech Group; 13763-1-AP) and α-tubulin (Cell Signalling Technologies #2125 ...
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bioRxiv - Neuroscience 2021Quote: ... Blots were probed with antibodies for goat anti-human TREM2 antibody, (1:1000, R&D, AF1828) and mouse anti-h-TDP-43 (1:1000, Proteintech, 60019-2-Ig).
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bioRxiv - Pathology 2020Quote: ... the cells were incubated with sheep anti-human collagen I alpha 1 antibody (1:200) or rabbit-anti-α-SMA antibody (1:200, Proteintech, United States) at 4°C overnight ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: H&E staining and IHC were performed on paraffin sections using the following antibodies: anti-human CD41 (Proteintech, 24552-2-AP, 1:200), anti-human CD68 (Thermo Fisher ...
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bioRxiv - Biochemistry 2020Quote: ... Probing was performed for 2 hours with the following primary antibodies at 1:1000 in blocking buffer: rabbit anti-gamma tubulin (Proteintech, Rosemont IL, 15176-1-AP) and mouse anti-ARL13B (Proteintech ...
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bioRxiv - Cell Biology 2020Quote: ... electrotransferred to nitrocellulose membranes blocked with 5% (w/v) nonfat dry milk and incubated with primary antibodies against CD81 (18250-1-AP, Proteintech Group, Wuhan, P. R. China)(diluted as 1:2500) ...
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bioRxiv - Neuroscience 2020Quote: ... 25~30ug of total protein was resolved in 10% Nupage Bis-Tris gel and probed with followed primary antibodies: mouse monoclonal anti-KCNQ2 antibody (Proteintech, 66774-1-Ig, 1: 1,000), rabbit polyclonal anti-KCNQ3 antibody (Proteintech ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were blocked in 5% milk in PBS with 0.1% Tween®20 (PBS-T) for 30 min prior to incubation with specific primary antibodies: mouse anti-IFITM1 (Proteintech, 60074-1-Ig, 1:5000), rabbit anti-IFITM2 (Proteintech ...
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bioRxiv - Cell Biology 2019Quote: ... Immunocytochemistry was performed as previously described (Scheibinger et al., 2018) using the following primary antibodies: rabbit anti-TMSB4X (1:250, Proteintech, Rosemont, IL, 19850-1-AP); rabbit anti-AGR3 (1:250 ...
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bioRxiv - Immunology 2022Quote: ... Membranes were blocked with TBS/0.05% Tween-20/5% skim milk and then incubated with primary antibodies against p53 (Proteintech, Cat.No. 10442-1-AP, 1:1000), p21 (Proteintech ...
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bioRxiv - Biochemistry 2023Quote: ... proteins were transferred to nitrocellulose membranes and probed with specific primary antibodies against the following proteins: β-ACTIN (dilution 1:2,000; Proteintech; Rosemont, IL; 60008-1- Ig), ATP5A (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... The membranes were blocked with 5% non-fat milk in PBST and then incubated with primary antibodies against the PARIS (Proteintech, #24542-1-AP, 1:2500), PGC-1α (Novus ...
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bioRxiv - Bioengineering 2023Quote: ... Tissue sections were blocked in 200 uL blocking solution (1X PBS/0.3% Triton X-100/5% BSA) at room temperature for 1 hr and then incubated in primary antibody (Proteintech rabbit anti-PGP9.5 (14730-1-ap), 1:1000 ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... for 1 hr at room temperature and then incubated in primary antibodies (rabbit anti-Iba1, Abcam, ab178847, 1:500; rabbit anti-GFAP, Abcam, ab7260, 1:500; rabbit anti-DCX, Proteintech, 13925-1-AP, 1:200) diluted in blocking buffer at 4°C for 24 h ...
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bioRxiv - Neuroscience 2023Quote: ... followed by incubation overnight at 4°C in primary antibodies diluted in 5% (w/v) BSA in Tris buffered saline: anti-cofilin 1G6A2 (Proteintech, 66057-I-Ig, 1:3000), anti-phospho-cofilin (Ser3 ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies were raised against TDP-43 (1:2000 pan-specific rabbit anti-TDP-43 ProteinTech #10782-2-AP; RRID # AB_615042 or 1:2000 human-specific mouse anti-TDP-43 ProteinTech #60019-2-Ig; RRID #AB_2200520), β-actin (1:2000 ...
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bioRxiv - Microbiology 2023Quote: ... containing 4% nonfat milk (blocking buffer) for 1 hr at room temperature and probed with primary antibodies against Rab5B (Proteintech, Cat# 27403-1-AP, 1:1,000), SNX1 (Proteintech ...
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bioRxiv - Physiology 2023Quote: ... The membranes were blocked in 5% skim milk/TBST for 60 min at room temperature and were then incubated overnight at 4 °C with the primary antibodies [anti-RBM3 antibody (Proteintech, Cat#14363-1-AP, 1:1000) and anti-Tubulin antibody (SIGMA ...
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bioRxiv - Neuroscience 2021Quote: ... human specific TDP43 (a mouse monoclonal against the C-terminus of TDP-43 that recognises human TDP-43 protein but not mouse TDP-43, Proteintech 60019-2-Ig, 1:1000), pan SOD1 (a custom-made goat polyclonal antibody recognising a C-terminal epitope of SOD1 that is identical between mouse and human proteins ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies used for immunoblotting were rabbit anti-TDP-43 polyclonal antibody (PAb) for detecting both human and mouse TDP-43 (Proteintech #10782-2-AP, 1:2000), rat anti-phospho-S409/410 TDP-43 monoclonal antibody (MAb ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies used for IF were rabbit anti-TDP-43 polyclonal antibody (PAb) for detecting pan-TDP-43 for both human and mouse TDP-43 (Proteintech #10782-2-AP, 1:1000), and rat anti-glial fibrillary acidic protein (GFAP ...
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bioRxiv - Neuroscience 2023Quote: ... Sections of 40 μm thickness were cut on a cryostat and incubated with primary antibodies to Rabbit anti-GFAP (Proteintech 16825-1-AP, used at 1:200) and Rat anti-Cd11b (ThermoFisher 12-0112-82 ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were stained using anti-ARL13B (Proteintech) at 1:6000 ...
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bioRxiv - Immunology 2021Quote: ... Gasdermin D (Proteintech and Cell Signaling Technologies), caspase-1 (AdipoGen Life Sciences) ...
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bioRxiv - Neuroscience 2023Quote: ... (Cell Signaling Technologies, R&D, Proteintech, Abcam). Only the single banding pattern between 100 kDa-75 kDa was present in ganglia and is therefore used in the validation blot for the antibody and to verify presence in ganglia ...