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Citations for Proteintech :
3251 - 3300 of 3383 citations for Goat Anti Human IgG Fc Alkaline Phosphatase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... and incubated with the following primary antibodies diluted in Odyssey Antibody Diluent (Li-COR): anti-IFITM1 (60074-1-Ig; Proteintech), anti-IFITM2 (66137-1-Ig ...
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bioRxiv - Microbiology 2022Quote: ... Whole cell lysate and supernatant of two biological replicates were probed by western blotting using a 1:1000 dilution anti-mCherry antibody (Proteintech) as a primary antibody and a 1:30000 dilution goat anti-rabbit antibody as a secondary antibody ...
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bioRxiv - Cell Biology 2024Quote: ... anti-NME3 (Cat#15136-1-AP, 1:500), and anti-CHOP (Cat#15204-1-AP, 1:1000) antibodies was obtained from Proteintech Group ...
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bioRxiv - Molecular Biology 2024Quote: ... incubate overnight at 4°C using the following primary antibody incubation protocol: rabbit anti-Collagen I (Proteintech,14695-1-AP), rabbit anti-Collagen IV (Proteintech,55131-1) ...
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bioRxiv - Cell Biology 2023Quote: ... Rabbit anti-MYH6 and MYH7 for western blotting (1:500) were purchased from ProteinTech (22281-1-AP, 22280-1-AP).
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bioRxiv - Cell Biology 2024Quote: ... indicated cells were washed with phosphate-buffered saline (PBS) and stained with mouse anti-ACE2 (# 66699-1-Ig, Proteintech, China) at a 1:50 dilution for 30 min at 4 °C ...
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bioRxiv - Developmental Biology 2024Quote: ... Slides were blocked in 10% normal donkey serum and then stained with monoclonal anti-FN1 antibody (1:200; 15613-1-AP, Proteintech), followed by Alexa Fluor 488–conjugated goat anti-rabbit antibody (1:500 ...
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bioRxiv - Microbiology 2024Quote: ... The cells were then lysed with SDS sample loading buffer before being analyzed by SDS-PAGE and immunoblotting with anti-His tag monoclonal antibody (1:1000; Cat# HRP-66005, Proteintech).
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bioRxiv - Microbiology 2024Quote: ... Cells were harvested and lysed overnight with SDS sample loading buffer before being subjected to SDS-PAGE and immunoblot analysis using anti-His tag monoclonal antibody (1:1000; Cat# HRP-66005, Proteintech).
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bioRxiv - Cell Biology 2023Quote: ... Cells lysates were either mixed with 3 × Laemmli buffer for immunoblotting analysis or subject to pre-clearing and NDC80 complexes were isolated from 1 mg of cell lysate by 2-h incubation at 4°C with anti-NDC80 antibody cocktail (ProteinTech, 18932-1-AP ...
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bioRxiv - Cell Biology 2023Quote: Endogenous GM130 was immunoprecipitated by incubation of cleared SV589 cell lysates for 1 h at 4°C with either 1 µg rabbit anti-GM130 antibodies (Proteintech), 5 µl rabbit polyclonal anti- GM130 serum (Wei and Seemann ...
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bioRxiv - Molecular Biology 2023Quote: ... Superase-in (1 U/ml) (Thermo) in nuclease free H2O) were tumbled with 5 μg anti-NOP58 antibody (#14409-1-AP, Proteintech) or 5 μg anti-IgG antibody (#30000-0-AP ...
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bioRxiv - Neuroscience 2023Quote: ... which were blocked in 5% dry milk powder in TBS-T for 1 hour and then incubated in primary antibody solutions (Rabbit anti NUDT6, Proteintech 1:800 ...
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bioRxiv - Cancer Biology 2023Quote: ... Equal amount of chromatin was incubated overnight at 4°C with 2 µg of anti-MondoA (Proteintech, 13614-1-AP) or IgG (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2023Quote: ... Scaffolds were blocked for 1 hr in 1% BSA/10% FBS and then co-stained with anti-desmin (1:100; cat# 16520-1-AP; Proteintech) and anti-Laminin-2 (1:100 ...
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bioRxiv - Cancer Biology 2023Quote: ... the membranes were incubated overnight at 4 °C with primary antibodies either rabbit anti-ASNS antibody (Proteintech, 14681-1-AP) diluted 1:1,000 in 5% BSA (Sigma) ...
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bioRxiv - Biochemistry 2023Quote: ... 0.1 % Tween in PBS) followed by a 2 h incubation at 37 °C with the respective primary antibody (anti-G3BP2, 1:600 [Proteintech® ...
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bioRxiv - Biochemistry 2023Quote: ... complexes containing GFP-tagged PPP1R15A were recovered on a resin coated with anti-GFP nanobodies (GFP-Trap, Proteintech, Planegg, Germany), following lysis in the manufacturer’s recommended buffer supplemented with 100 µM TCEP ...
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bioRxiv - Neuroscience 2023Quote: ... blocked with 5% FBS and stained overnight at 4°C with primary antibodies for immunocytochemical analysis (rabbit anti-OCT4, 1:100, Proteintech, Cat ...
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bioRxiv - Molecular Biology 2023Quote: ... The membranes were then incubated overnight at 4°C with anti-DYKDDDDK antibody (66008-3-Ig, 1:2000 dilution, Proteintech) in Western Primary Antibody Dilution Solution (Beyotime Biotech ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... followed by overnight incubation at 4°C with primary antibodies diluted in blocking solution: mouse anti-p62 (Proteintech; 1:100), Thereafter ...
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Aberrant regulation of serine metabolism drives extracellular vesicle release and cancer progressionbioRxiv - Cancer Biology 2024Quote: ... anti-PHGDH antibody (14719-1-AP, dilution 1:1000) and anti-PSPH antibody (14513-1-AP, dilution 1:1000) were purchased from Proteintech Group ...
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bioRxiv - Cancer Biology 2024Quote: ... heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 min following incubation with 1:100 dilution of the primary antibody anti- Piezo1 (28511- 1-AP, Proteintech). Samples were scored in five levels of PIEZO1 expression in the tumor (0 to 4) ...
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bioRxiv - Neuroscience 2024Quote: ... For overnight incubation at +4°C the following primary antibodies were used: anti-TDP-43 (1:100, 10782-2-AP, Proteintech), anti-phospho-TDP-43 (1:200 ...
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bioRxiv - Genetics 2021Quote: ... in PBS with 0.05% Tween 20 (PBST) for 1 h and then incubated with anti-Collagen I-α2 (Proteintech, 1:1000), anti-α-SMA (Proteintech ...
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bioRxiv - Cancer Biology 2022Quote: ... For ZBTB18 co-IP in BTSC3082 and BTSC268 cells,lysates were incubated overnight with rabbit anti-ZBTB18 (Proteintech #12714-1-AP). For mass spectrometry (MS ...
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bioRxiv - Molecular Biology 2021Quote: ... Membrane was saturated with 5% low-fat milk in Tris-buffered saline containing tween 0.1% during 1 hour at RT and then probed with primary antibodies: anti-Piezo1 (rabbit, Proteintech, 1/1000), anti-KCNMA1 (rabbit ...
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bioRxiv - Molecular Biology 2020Quote: ... Immunoblotting was performed using rabbit polyclonal anti-SM(SQLE) (Proteintech 12544-1-AP; 1:2,500 at 4°C for 16 h), rabbit monoclonal anti-GAPDH (Cell Signaling Technology 2118 ...
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bioRxiv - Molecular Biology 2020Quote: ... The primary antibodies were as follows: anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH; cat. no. 60004-1-lg; Proteintech, Rosemont, IL, USA), mouse monoclonal anti-β-actin (cat ...
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bioRxiv - Biochemistry 2019Quote: ... His-tagged RmtC proteins were detected by immunoblotting with a rabbit anti-6×His antibody (α6×His; Proteintech; 10001-0-AP) overnight at 4 °C ...
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bioRxiv - Cell Biology 2019Quote: ... The amount of motors in COS-7 lysates was determined by Dot Blot using an anti-GFP antibody (1:10,000, ProteinTech, #66002-1). Digital images of the blots were analyzed by ImageJ ...
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bioRxiv - Cell Biology 2019Quote: ... The primary antibodies were incubated overnight at 4°C and are as follows: mouse anti-MCL-1 (Proteintech # 66026-1-Ig), rabbit anti-DRP-1 (Cell Signaling Technologies #8570S) ...
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bioRxiv - Biophysics 2022Quote: ... Equal quantities of protein were subjected to 15% SDS-PAGE followed by immunoblotting with anti-SN25 antibody (Proteintech; 14903-1-AP) (Figure 3A) ...
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bioRxiv - Microbiology 2021Quote: ... Lysed samples were centrifuged and supernatants were immunoprecipitated with 5µg/ml mouse monoclonal anti-IFITM2/3 antibody (Proteintech, 66081-1-Ig) for 1.5 h at 4°C ...
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bioRxiv - Systems Biology 2022Quote: ... anti-TIMM44 and TOMM70 (1:500, 1:1000, 1:500, rabbit, ProteinTech, 16139-1-AP, 13859-1-AP, 14528-1-AP); anti-TIMM23 (1:1000 ...
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bioRxiv - Immunology 2022Quote: ... The membrane containing the total cell extract was incubated with HRP-conjugated anti-GAPDH mouse monoclonal antibody (HRP-60004, ProteinTech, USA). The HRP signal was revealed by ChemiFast Chemiluminescent Substrate (CH-FAST/20 ...
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bioRxiv - Cell Biology 2021Quote: ... 800-μg lysates were suspended in 400-μl lysis buffer and immunoprecipitated with 1-μg anti–INPP5E antibody (Proteintech, Rosemont, IL) overnight ...
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bioRxiv - Cell Biology 2021Quote: ... Anti-fibronectin 1 antibody (15613-1-AP) and anti-ERGIC-53 (13364-1-AP, RRID:AB_2135994) were purchased from Proteintech (Rosemont, IL, USA). Anti-SEC24B antibody (A304-876A ...
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bioRxiv - Cancer Biology 2021Quote: ... membranes were probed with primary antibodies overnight at 4°C at dilutions recommended by the suppliers as follows: anti-Alix (Proteintech, 12422), TSG101 (Proteintech ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated overnight at 4°C with the primary rabbit anti-ataxin-3 (1:200; ProteinTech, Rosemont, IL, USA), mouse SMI-32 against non-phosphorylated heavy and medium neurofilament subunits (1:1000 ...
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bioRxiv - Cell Biology 2022Quote: ... PVDF membranes were incubated overnight at 4°C with the following primary antibodies: rabbit anti-G-6-PD (1:5000, Proteintech, China), rabbit anti-GLUT-2 (1:1000 ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was mixed with 0.1 μg/μL RNase A or 1 unit/μL RNasin Plus and incubated with 1 μg of anti-ribosomal protein P0 antibody (isotype: mouse IgG2a κ, clone:9D5) (RN004M, MBL) or isotype control antibody (65208-1-IG, ProteinTech) at 4ºC for 60 min ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was used for a western-blot experiment using a mouse-anti-Phospho-eIF2α (Ser 51, 1:5,000, Proteintech 68023-1). To detect primary Ab ...
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bioRxiv - Cancer Biology 2024Quote: Mitochondria were imaged using BacMam CellLightTM Mitochondria-RFP (fused construct of E1 alpha pyruvate dehydrogenase leader sequence and TagRFP) (ThermoFischer, C10601) and anti-TOM20-GFP antibodies (Proteintech, #CL48811802). Endosomes ...
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bioRxiv - Molecular Biology 2024Quote: ... anti-CD11B/integrin alpha M (rabbit polyclonal, #21851-1-AP, RRID: AB_2878927, Proteintech; 1:200 for IHC 1:2000 for WB), anti-RAB5A (rabbit polyclonal ...
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bioRxiv - Genomics 2024Quote: ... Membranes were blocked with 5% milk in TBST for 1 hour at room temperature followed by incubation with 1:500 anti-UPF1 antibody (Proteintech #66898) or 1:5000 anti-Tubulin (Sigma-Aldrich #T6199 ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then incubated overnight at 4°C in rabbit anti-Rab35 (1:100; Cat. 11329-2-AP, Proteintech, Rosemont, IL) or for 1 h at room temperature (RT ...
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bioRxiv - Cancer Biology 2023Quote: ... were performed as previously reported.43 The primary antibodies were anti-topoisomerase 2β) (TOP2β, 1:1000 dilution, #20549-1-AP, Proteintech, USA), anti-fibroblast activation protein alpha (FAP ...
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bioRxiv - Cell Biology 2023Quote: ... anti-TNFR2 (28746-1-AP, 1:200 dilution) and anti-GAPDH (60004-4-I-Ig, 1:5,000 dilution) were purchased from Proteintech (Wuhan, China). Anti-SARS-CoV/SARS-2-S (GTX632604 ...
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bioRxiv - Neuroscience 2023Quote: ... GAPDH-71.1).The following antibodies were used for i3Neuron stable cell lines immunoblot: Rabbit anti-TDP-43 (Proteintech 10782-2-AP 1:1000); Mouse anti-FLAG (Sigma F1804 ...