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• PP6 regulation of Aurora A-TPX2 limits NDC80 phosphorylation and mitotic spindle size

  Tomoaki Sobajima, et al., bioRxiv - Cell Biology 2023

  Quote: ... Cells lysates were either mixed with 3 × Laemmli buffer for immunoblotting analysis or subject to pre-clearing and NDC80 complexes were isolated from 1 mg of cell lysate by 2-h incubation at 4°C with anti-NDC80 antibody cocktail (ProteinTech, 18932-1-AP ...
• Tumor cell-adipocyte gap junctions activate lipolysis in breast cancer

  Roman Camarda, et al., bioRxiv - Cancer Biology 2023

  Quote: ... and incubated with primary antibodies (Cx31, 12880, Proteintech, 1:50 and pan-cytokeratin ...
• Self-organization, quality control, and preclinical studies of human iPSC-derived retinal sheets for tissue-transplantation therapy

  Kenji Watari, et al., bioRxiv - Neuroscience 2023

  Quote: ... anti-Recoverin (rabbit; 1:500; Proteintech), anti-RXRG (mouse ...
• Single nucleus multiome analysis of the prefrontal cortex from C9orf72 ALS/FTD patients illuminates pathways affected during disease progression

  Hsiao-Lin V. Wang, et al., bioRxiv - Neuroscience 2023

  Quote: ... and the detection antibody was a sulfo-tagged rabbit polyclonal C-terminal TDP-43 antibody (2 μg/mL, 12892-1-AP, Proteintech). Lysates were diluted in 8 M urea such that all samples of a given type were made up to the same concentration and an equal amount of protein for samples was tested in duplicate wells ...
• VAP spatially stabilizes dendritic mitochondria to locally fuel synaptic plasticity

  Ojasee Bapat, et al., bioRxiv - Neuroscience 2023

  Quote: ... anti-VAPB rb (Proteintech 144771AP, 1:100) for VAP immunoamplification ...
• An axon – T cell feedback loop enhances inflammation and axon degeneration

  Tingting Liu, et al., bioRxiv - Neuroscience 2023

  Quote: ... The primary antibodies used in this study were rabbit anti-PGP9.5 (ProteinTech Group, RRID:AB_2210497), and rabbit anti-TH (Millipore ...
• Neuronal activity-driven O-GlcNAcylation promotes mitochondrial plasticity

  Seungyoon B. Yu, et al., bioRxiv - Neuroscience 2023

  Quote: ... rabbit anti-OGA (MGEA5) (Proteintech), mouse anti-tubulin (Sigma-Aldrich) ...
• Lack of Fzd6 in Ciliated Cells Suppresses Ferroptotic Pulmonary Alveolar Cell Death Induced by LPS and Coronavirus

  Qianying Yuan, et al., bioRxiv - Pathology 2023

  Quote: ... IL-1b and TNF-a were determined by using enzyme-linked immunosorbent assay (ELISA) kits specific for the cytokines according to the manufacturer’s instructions (Proteintech).
• LIFR recruits HGF-producing neutrophils to promote liver injury repair and regeneration

  Yalan Deng, et al., bioRxiv - Cell Biology 2023

  Quote: ... mouse TGF-β (Proteintech, KE10005), and mouse Cxcl1 (Sigma-Aldrich ...
• LIFR recruits HGF-producing neutrophils to promote liver injury repair and regeneration

  Yalan Deng, et al., bioRxiv - Cell Biology 2023

  Quote: ... cyclin A2 (1:1000, Proteintech, 18202-1-AP, RRID: AB_10597084), phospho- Stat3 (Tyr705 ...
• LIFR recruits HGF-producing neutrophils to promote liver injury repair and regeneration

  Yalan Deng, et al., bioRxiv - Cell Biology 2023

  Quote: ... Gapdh (1:2,000, Proteintech, 60004-1- IG, RRID: AB_2107436), cyclin D1 (1:1,000 ...
• LIFR recruits HGF-producing neutrophils to promote liver injury repair and regeneration

  Yalan Deng, et al., bioRxiv - Cell Biology 2023

  Quote: ... Primary antibodies used are as follows: antibodies against Lifr (1:1,000, Proteintech, 22779-1-AP, RRID: AB_2879165), Gapdh (1:2,000 ...
• Tetracycline-dependent inhibition of mitoribosome protein elongation in mitochondrial disease mutant cells suppresses IRE1α to promote cell survival

  Conor T. Ronayne, et al., bioRxiv - Cell Biology 2023

  Quote: ... SRP14 (Proteintech, 11528-1-AP,1:1000), Calnexin (Cell Signaling ...
• RNA scaffolds the Golgi ribbon by forming condensates with GM130

  Yijiun Zhang, Joachim Seemann, bioRxiv - Cell Biology 2023

  Quote: Endogenous GM130 was immunoprecipitated by incubation of cleared SV589 cell lysates for 1 h at 4°C with either 1 µg rabbit anti-GM130 antibodies (Proteintech), 5 µl rabbit polyclonal anti- GM130 serum (Wei and Seemann ...
• ODF2 negatively regulates CP110 levels at centrioles/basal bodies to control biogenesis of primary cilia

  Madeline Otto, Sigrid Hoyer-Fender, bioRxiv - Cell Biology 2023

  Quote: ... anti-CP110 (#12780-1-AP, diluted 1:100, Proteintech, St. Leon-Rot, Germany), or anti-GFP (either monoclonal mouse IgG2a (3E6) ...
• ODF2 negatively regulates CP110 levels at centrioles/basal bodies to control biogenesis of primary cilia

  Madeline Otto, Sigrid Hoyer-Fender, bioRxiv - Cell Biology 2023

  Quote: ... cells were incubated with anti-CP110 antibodies (#12780-1-AP, diluted 1:100, Proteintech, St. Leon-Rot, Germany) followed by goat anti-rabbit-IgG-AlexaFluor-647 (#A-21245 ...
• Depletion of LONP2 unmasks differential requirements for peroxisomal function between cell types and in cholesterol metabolism

  Akihiro Yamashita, et al., bioRxiv - Cell Biology 2023

  Quote: ... RRS1 (Proteintech, 15329-1-AP), Thiolase (Sigma ...
• Depletion of LONP2 unmasks differential requirements for peroxisomal function between cell types and in cholesterol metabolism

  Akihiro Yamashita, et al., bioRxiv - Cell Biology 2023

  Quote: ... CRABP2 (Proteintech, 10225-1-AP), GAPDH (Abcam ...
• Depletion of LONP2 unmasks differential requirements for peroxisomal function between cell types and in cholesterol metabolism

  Akihiro Yamashita, et al., bioRxiv - Cell Biology 2023

  Quote: ... LONP1 (Proteintech, 15440-1-AP), NF-κB (Cell Signaling ...
• Efferocytosis-Induced Lactate Enables the Proliferation of Pro-Resolving Macrophages to Mediate Tissue Repair

  David Ngai, Maaike Schilperoort, Ira Tabas, bioRxiv - Immunology 2023

  Quote: ... rabbit anti-LDHA (ProteinTech 19987-1-AP; 1:1000), rabbit anti-pAMPK (CST 2535 ...
• Efferocytosis-Induced Lactate Enables the Proliferation of Pro-Resolving Macrophages to Mediate Tissue Repair

  David Ngai, Maaike Schilperoort, Ira Tabas, bioRxiv - Immunology 2023

  Quote: ... rabbit anti-LDHA (ProteinTech 19987-1-AP; 1:100), rabbit anti-Ki67 (ab16667 ...
• Neuroimmune characterization of optineurin insufficiency mouse model during ageing

  Nikolina Mohovic, et al., bioRxiv - Immunology 2023

  Quote: ... rabbit N-terminal anti-TDP-43 (#10782-2-AP) and rabbit C-terminal anti-TDP-43 (#12892-1-AP) from Proteintech (Manchester, UK); goat anti-ChAT (#AB144P) ...
• ROS-dependent palmitoylation is an obligate licensing modification for GSDMD pore formation

  Gang Du, et al., bioRxiv - Immunology 2023

  Quote: ... 1:500 rabbit anti-zDHHC5 polyclonal antibody (Proteintech, 21324-1-AP), 1:500 rabbit anti-zDHHC9 polyclonal Antibody (ABclonal ...
• Tetracycline-dependent inhibition of mitoribosome protein elongation in mitochondrial disease mutant cells suppresses IRE1α to promote cell survival

  Conor T. Ronayne, et al., bioRxiv - Cell Biology 2023

  Quote: ... MRPL4 (Proteintech, 27484-1-AP, 1:1000), MRPS16 (Proteintech ...
• Vitamin D inhibits osteosarcoma by reprogramming nonsense-mediated RNA decay and SNAI2-mediated epithelial-to-mesenchymal transition

  Enrico Capobianco, et al., bioRxiv - Cancer Biology 2023

  Quote: ... The primary antibodies were diluted in a buffer of 0.5% milk/1X TBST (CD44 antibody; 15675-1-AP, Proteintech). The Li-COR system was used to detect antibody binding.
• Signaling protein abundance modulates the strength of the Spindle Assembly Checkpoint

  Chu Chen, et al., bioRxiv - Cell Biology 2023

  Quote: ... anti-GAPDH (Proteintech 60004-1-Ig, 1 : 5000).
• Mice with renal-specific alterations of stem cell-associated signaling develop symptoms of chronic kidney disease but surprisingly no tumors

  Adam Myszczyszyn, et al., bioRxiv - Cell Biology 2023

  Quote: ... Sections were incubated overnight with rabbit anti-Pax8 primary antibody (1:2000, Proteintech, 10336-1-AP), followed by incubation with peroxidase-conjugated donkey anti-rabbit secondary antibody from a kit (Dako ...
• Tau tubulin kinase 1 and 2 regulate ciliogenesis and human pluripotent stem cells–derived neural rosettes

  Lucia Binó, Lukáš Čajánek, bioRxiv - Cell Biology 2023

  Quote: ... α-TUBULIN (mouse, 6631-1 Proteintech, 1:2000) was used as a loading control ...
• eIF2B localisation and its regulation during the integrated stress response is cell type specific

  Filipe M. Hanson, et al., bioRxiv - Cell Biology 2023

  Quote: ... as following: eIF2Bα (1:25, 18010-1-688 AP, Proteintech), eIF2Bβ (1:25 ...
• eIF2B localisation and its regulation during the integrated stress response is cell type specific

  Filipe M. Hanson, et al., bioRxiv - Cell Biology 2023

  Quote: ... eIF2Bβ (1:25, 11034-1-AP, Proteintech), eIF2Bγ (1:50 ...
• Whole mouse body histology using standard IgG antibodies

  Hongcheng Mai, et al., bioRxiv - Biochemistry 2023

  Quote: ... PGP9.5 (Proteintech, 14730-1-AP), LYVE1 (Thermo Fisher Scientific ...
• Co-chaperone BAG3 directly target autophagic degradation via its LC3-interacting regions

  Hagen Körschgen, et al., bioRxiv - Biochemistry 2023

  Quote: ... incubated with 2 μl anti-BAG3 antibody (Proteintech, 10599-1-AP) at 4°C and precipitated with fresh beads ...
• Rigosertib promotes anti-tumor immunity via autophagic degradation of PD-L1 in colorectal cancer cells

  Xinyi Zhou, et al., bioRxiv - Cancer Biology 2023

  Quote: ... anti-PD-L1 antibody (1:500; Proteintech, 66248-1-Ig), and anti-P62 antibody (1:500 ...
• YAP-controlled MHC-I and CXCL10 expression potentiates antitumor immunity independently of IFNγ signaling

  Linyuan Peng, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Actin (Proteintech, 66009-1-1g)) ...
• ZC3H11A interacts with PABPN1 and alters polyadenylation of viral transcripts

  Katharina Kases, et al., bioRxiv - Molecular Biology 2023

  Quote: ... anti-GAPDH (mouse, Proteintech, 60004-1-Ig), anti-PABP (rabbit ...
• A chemical probe to modulate human GID4 Pro/N-degron interactions

  Dominic D.G Owens, et al., bioRxiv - Molecular Biology 2023

  Quote: ... and DDX50 (Proteintech Cat# 10358-I-AP, 1:400). Cells were washed three times with 1× PBS ...
• Development of Multiomics in situ Pairwise Sequencing (MiP-Seq) for Single-cell Resolution Multidimensional Spatial Omics

  Xiaofeng Wu, et al., bioRxiv - Molecular Biology 2023

  Quote: ... Flag (Proteintech, 66008-3-Ig), CSFV E2 (generous gift by Wuhan Keqian Biological) ...
• Ribonucleoprotein condensation driven by retrotransposon LINE-1 sustains RNA integrity and translation in mouse spermatocytes

  Chiara De Luca, Anuj Gupta, Alex Bortvin, bioRxiv - Molecular Biology 2023

  Quote: ... rabbit polyclonal anti-CNOT1 (Proteintech, 14276-1-AP) diluted 1:200 for immunofluorescence ...
• Antagonism among DUX family members evolved from an ancestral toxic single homeodomain protein

  Darko Bosnakovski, et al., bioRxiv - Molecular Biology 2023

  Quote: ... Antibodies used in the study: GAPDH-HRP (1:5000, 60004, Proteintech), rabbit anti-Histone H3K18Ac (1:500 ...
• DiPRO1 dependent transcriptional and epigenetic regulation distinctly controls the fate of muscle and mesenchymal cancer cells

  Jeremy Rich, et al., bioRxiv - Molecular Biology 2023

  Quote: Polyclonal rabbit anti-MCM6 (Proteintech, 13347-2-AP) and polyclonal rabbit anti-TIF1B/KAP1 (Merck Millipore ...
• Pathway choice in the alternative telomere lengthening in neoplasia is dictated by replication fork processing mediated by EXD2’s nuclease activity

  Ronan Broderick, et al., bioRxiv - Molecular Biology 2023

  Quote: ... RAD52 (28045-1-AP, Proteintech, 1:2000), SMARCAL1 (Santa Cruz ...
• Differential effects of pre-mRNA splicing inhibitors on RNA polymerase II transcription

  Michael Tellier, Gilbert Ansa, Shona Murphy, bioRxiv - Molecular Biology 2023

  Quote: ... Proteintech SUMO1 Monoclonal antibody (67559-1-IG-20UL, Proteintech Europe), Proteintech SUMO2/3 Polyclonal antibody (11251-1-AP-20UL ...
• Differential effects of pre-mRNA splicing inhibitors on RNA polymerase II transcription

  Michael Tellier, Gilbert Ansa, Shona Murphy, bioRxiv - Molecular Biology 2023

  Quote: ... Proteintech SUMO2/3 Polyclonal antibody (11251-1-AP-20UL, Proteintech Europe), and Histone H3 (ab1791 ...
• MRE11A: a novel negative regulator of human DNA mismatch repair

  Demin Du, et al., bioRxiv - Molecular Biology 2023

  Quote: ... PMS2 (Proteintech, 66075-1-Ig), MSH6 (Proteintech ...
• MRE11A: a novel negative regulator of human DNA mismatch repair

  Demin Du, et al., bioRxiv - Molecular Biology 2023

  Quote: ... actin (Proteintech, 20536-1-AP), P-CHK2-Thr68 (CST ...
• MRE11A: a novel negative regulator of human DNA mismatch repair

  Demin Du, et al., bioRxiv - Molecular Biology 2023

  Quote: ... MSH2 (Proteintech, 15520-1-AP) MRE11A (Proteintech ...
• Amino acid substitutions in norovirus VP1 dictate cell tropism via an attachment process dependent on membrane mobility

  Jake T. Mills, et al., bioRxiv - Microbiology 2023

  Quote: ... and anti-BSA monoclonal antibody (66201-1, ProteinTech). Polyclonal anti-mouse (PA1-84388 ...
• Amino acid substitutions in norovirus VP1 dictate cell tropism via an attachment process dependent on membrane mobility

  Jake T. Mills, et al., bioRxiv - Microbiology 2023

  Quote: ... anti-GAPDH monocolonal (60004-1, ProteinTech) and anti-BSA monoclonal antibody (66201-1 ...
• Single-residue mutation in protein kinase C toggles between cancer and neurodegeneration

  Alexander C. Jones, et al., bioRxiv - Molecular Biology 2023

  Quote: ... GFP-Trap® Agarose or control agarose (Proteintech, product code ...
• An O-GlcNAc transferase pathogenic variant that affects pluripotent stem cell self-renewal

  Michaela Omelková, et al., bioRxiv - Molecular Biology 2023

  Quote: ... and α-Tubulin (Proteintech, catalogue number 11224-1-AP, 1:5000). After incubation with the corresponding LI-COR secondary antibodies (1:10 000) ...