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Citations for Proteintech :
5201 - 5250 of 5255 citations for 6 Methylimidazo 1 2 a pyridine HCl since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... The primary antibody for RBP3 (also known as IRBP) was rabbit anti-RBP3 polyclonal (Proteintech, Catalog No. 14352-1-AP, Illinois). The AdipoR1 RNAScope fluorescent assay was executed using an Automated Leica Bond RX ...
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bioRxiv - Cell Biology 2019Quote: ... The blots were blocked in 4% BSA in TBST solution for 30 min at room temperature and then incubated at 4-°C overnight with the primary antibody: HSP90α(Proteintech, 1:1000), HIF-1α(CST ...
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bioRxiv - Pathology 2019Quote: ... The membranes were incubated for 1hr in blocking buffer (Odyssey) at room temperature before incubation with primary MMP-13 rabbit polyclonal antibody (ProteinTech, 1:333), and monoclonal beta-actin antibody (Abcam ab8226 ...
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bioRxiv - Neuroscience 2021Quote: ... The membrane was blocked in 4% milk-PBST and proteins were stained using the following antibodies: anti-TDP-43 (rabbit, Proteintech, 1:1000), anti-GAPDH (rabbit ...
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bioRxiv - Physiology 2020Quote: ... The lysates were then centrifuged for 15 min at 16,000 x g and the supernatants were collected for immunoblot analysis with antibodies for TMPRSS2 (Abcam: ab92323 and Proteintech: 14437-1-AP), AR (EMD Millipore ...
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bioRxiv - Cell Biology 2022Quote: ... and equal amounts of protein were incubated with 25 uL RFP-Trap magnetic agarose rotating for 1 hour at 4°C (Proteintech, rta-10). The beads were washed three times and boiled in SDS sample buffer for 10 minutes at 95°C ...
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bioRxiv - Cancer Biology 2022Quote: ... incubated with primary antibodies overnight at 4 °C at dilutions recommended by the suppliers as follows: anti-TSG101 (14497, Proteintech, 1:1,000), anti-CD81 (66866 ...
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bioRxiv - Molecular Biology 2022Quote: ... first with AGAL and lysosome detected as described above and then with subsequent detection of TMED9 with rabbit polyclonal anti-TMED9 antibody (21620-1-AP, Proteintech, Manchester, UK) directly labeled with Daylight 488 Fast Conjugation Kit (Abcam ...
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Loss of the mitochondrial citrate carrier, Slc25a1/CIC disrupts embryogenesis via 2-HydroxyglutaratebioRxiv - Developmental Biology 2024Quote: ... sectioned in the mid sagittal plane and stained with hematoxylin and eosin (H&E) and anti-Slc25a1 antibody (Proteintech, 15235-1-AP), used at 1/150 dilution ...
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bioRxiv - Cell Biology 2024Quote: Antibodies directed against the following proteins were used in this study: SLC25A46 (Santa Cruz; G2-SC-515823 and Proteintech; 12277-1-AP), MFN2 (Cell Signaling ...
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bioRxiv - Neuroscience 2024Quote: ... cOmplete protease inhibitors) and subsequently incubated overnight at 4°C with 8 µg of either rabbit anti-Mef2c (Proteintech #18290-1-AP) or rabbit IgG isotype control (Abcam #ab27478) ...
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bioRxiv - Cancer Biology 2023Quote: ... Tween-20 PBS buffer) for three times and 100 μl of 1:5000 diluted HRP-conjugated anti-His antibody (Proteintech, HRP-66005) was added into each well and incubated at 4°C overnight or 2 h at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... Slides were blocked with 3% hydrogen peroxide and 5% horse serum and then incubated with the following primary antibodies: anti-METTL3 (1:100, Proteintech, Wuhan, China), anti-FTO (1:100 ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were blocked with 5% goat serum and then incubated with the following primary antibodies: anti-METTL3 (1:100, Proteintech, Wuhan, China), anti-FTO (1:100 ...
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bioRxiv - Molecular Biology 2023Quote: ... 1:1000 dilution), Anti-β Amyloid (D-11 Santacruz, Cat. Sc-374527, 1:1000) and anti-β-tubulin (Invitrogen Cat.32–2600 or Proteintech Europe ...
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bioRxiv - Pathology 2023Quote: ... membranes were blocked in 5% skimmed milk and proteins detected by primary antibodies overnight at 4℃: anti-αSMA (Proteintech, China,1:20000), anti-MCU (Proteintech ...
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bioRxiv - Molecular Biology 2024Quote: Lysate collection and immunoprecipitation were performed using the Magna RIP immunoprecipitation assay (MilliporeSigma) and 5 μg of rabbit anti-Caprin-1 (Proteintech, Rosemont, IL) or rabbit IgG control ...
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bioRxiv - Biochemistry 2024Quote: ... Membranes were blocked in Intercept PBS Blocking Buffer (Li-Cor Biotechnology, Cambridge, UK) before over-night incubation with anti-RNaseH1 (1:500, Proteintech, Manchester, UK) and anti-Vinculin (1:20,000 ...
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An mRNA processing pathway suppresses metastasis by governing translational control from the nucleusbioRxiv - Cancer Biology 2021Quote: ... Clarified lysates were pooled and RNA-protein complexes were then immunoprecipitated using protein A/G beads (Pierce) conjugated to anti-PABPC4 (Proteintech 14960-1-AP) for 2 hours at 4°C ...
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bioRxiv - Biochemistry 2019Quote: Native RNA immunoprecipitations were performed on one 150 mm dish of HEK293T cells as previously described45 using anti-DDX21 antibody (ProteinTech 15252-1-AP). RT-qPCR analysis was performed on Applied Biosystems StepOnePlus instrument with the RNA to Ct One-step RT-qPCR kit (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2019Quote: ... Efficiency of mRNA depletion was assessed 72 hours post-transfection using qPCR or western blot in the case of ACSL1 (Proteintech, 13989-1-AP). In experiments to determine suppression of cell toxicity resulting from αESA or ML162 by silencing expression of individual ACSL or ALOX genes ...
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bioRxiv - Molecular Biology 2022Quote: ... as a blocking buffer and then overnight at 4°C in the same blocking solution containing one of the following antibodies: anti-HAX1 (rabbit, Proteintech 11266-1-AP), anti-RPL26 (rabbit ...
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bioRxiv - Cell Biology 2022Quote: ... or using Dynabeads-protein G (Thermo) magnetic beads with primary antibodies (anti-GFP Genetex No#GTX113717; anti-Sec5 Proteintech No#12751-1-AP) cross-linked using dimethyl pimelimidate (Life Technologies).
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bioRxiv - Cancer Biology 2020Quote: ... coupled to 1 ml of Protein A agarose resin (Thermo-Fisher: 44893) or with 105 μg of normal rabbit IgG (Proteintech: 30000-0-A) coupled to 1 mL of Protein A agarose resin ...
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bioRxiv - Cancer Biology 2020Quote: ... The supernatant (500 μg of nuclear extract) was incubated for 16 hours at 4°C with 105 μg of PAX8-specific antibody (Proteintech: 10336-1-AP) coupled to 1 ml of Protein A agarose resin (Thermo-Fisher ...
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bioRxiv - Genetics 2022Quote: ... The cells were then fixed in 4%PFA and stained with rabbit monoclonal ARL13B primary antibody (17711-1-AP, Proteintech, Rosemont, Illinois, USA) and checked for ciliation before RNA extraction ...
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bioRxiv - Bioengineering 2022Quote: ... The nitrocellulose membrane was removed and placed in 1X Casein blocker for one hour on rotator followed by incubation with a 1:1000 dilution of his HRP-conjugated antibodies (Proteintech, Rosemount, IL, US) for 1 hour room temperature ...
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bioRxiv - Microbiology 2021Quote: ... We also purchased antibodies for cleaved caspase-1 (#AF4022) from Affinity Biosciences (Taizhou, China) and GAPDH and β-actin from Proteintech (Wuhan, China). An MTT Assay Kit was purchased from SunShineBio (Nanjing ...
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bioRxiv - Genomics 2021Quote: ... The 1mL lysate was divided into two tubes of 500µL and incubated overnight either with 5µL of rabbit IgG (Normal Rabbit IgG, #2729, 1mg/mL) or 5µL DDX55 (Bethy,l 1mg/mL, A303-027A) or 15µL TAF5L (Proteintech, 19274-1-AP, 0.333mg/mL) or 5µL TAF6L (ABclonal ...
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bioRxiv - Bioengineering 2021Quote: ... membranes were probed with primary antibodies overnight at 4 °C at dilutions recommended by the suppliers as follows: anti-Alix (Proteintech, 12422-1-AP), anti-TSG101 (Proteintech ...
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bioRxiv - Biophysics 2022Quote: A) Western blot of DMSO and dTAG-13 treated WT HeLa cells and TCOF1 degron cells using TCOF1 antibody (Proteintech, 11003-1-AP) and β-tubulin antibody as loading control ...
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bioRxiv - Cell Biology 2022Quote: ... and the resulting eluent and the first flow-through from the column were analyzed by Western blotting with antibodies against PSMC1 (Proteintech, 11196-1-AP), Ubiquilin 2 (Novus Biologicals ...
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bioRxiv - Biochemistry 2022Quote: ... The samples were eluted with LDS sample buffer for Western blot analysis and processed as described in the Western blotting section with primary antibodies for FLAG (R&D MAB8529) and HTRA1 (ProteinTech 55011-1-AP).
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bioRxiv - Biochemistry 2024Quote: ... During cell expansion a sample of each clone was processed into lysate and RNF126 knock-out clones were identified by anti-RNF126 immunoblotting (ProteinTech, #66647-1-Ig). Clone 2B8 was designated as the RNF126 knock-out.
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bioRxiv - Cancer Biology 2024Quote: ... incubated with Alexa-Fluor 594 goat anti-mouse IgG H&L or Alexa-Fluor 488 goat anti-rabbit IgG H&L (Proteintech, 1:400 dilution) at room temperature for 1 h in the dark ...
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bioRxiv - Cell Biology 2024Quote: Immunocytochemistry was performed as described elsewhere (Shelkovnikova et al, 2018) using the following commercially available antibodies: FBL (rabbit polyclonal, Proteintech, 16021-1-AP); NPM1 (mouse monoclonal ...
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bioRxiv - Physiology 2023Quote: ... Primary antibodies against phospho AKT(Ser473) (CST, 9271), AKT (AKT, 4685), HSP90 (CST, 4877), phospho IRF3(Ser396) (CST, 29047), IRF3 (CST, 11904) and AIG1 (Proteintech, 14468-1-AP), were used.
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bioRxiv - Cell Biology 2023Quote: ... HeLa cell lysates were mixed with mouse anti-NuSAP polyclonal antibody (provided by Dr. Zhaoxuan Deng) and rabbit anti-NuSAP polyclonal antibody (Proteintech, 12024-1-AP). For anti-Flag and anti-GFP IP assays ...
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bioRxiv - Cancer Biology 2024Quote: ... resuspended in HBSS containing 0.5% BSA and incubated for 30 min at 4 °C with 10 μg/ml anti-N-cad primary antibody (Proteintech, 22018-1-AP). Cells were washed twice in HBSS/0.5% BSA and incubated with Alexa Fluor 647-conjugated donkey anti-rabbit IgG (H+L ...
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bioRxiv - Cancer Biology 2024Quote: ... Coverslips were transferred to 4 °C and incubated in glioma migration medium containing 10 μg/ml rabbit anti-N-cad antibody (Proteintech, 22018-1-AP), which recognizes the N-cad extracellular domain ...
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bioRxiv - Neuroscience 2024Quote: ... 2024).To confirm the presence of Golgi-membrane proteins in the fractions, we used GM-130 (HuaBio, R1608-7) and CLINT1 (Proteintech, 10470-1-AP) as markers ...
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bioRxiv - Physiology 2020Quote: ... The following primary antibodies and dilutions were used and incubated in blocking buffer overnight at 4 °C with gentle rocking: BAG3 (Proteintech, 10599-1AP, 1:5,000), BAG3 (Santa Cruz Biotechnology ...
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bioRxiv - Microbiology 2020Quote: ... Protein gel was either stained with Coomassie G-250 (GelCode™ Blue Stain) or applied to western blotting of α1-AT with a polyclonal anti-α1-AT antibody (Proteintech 16382-1-AP). The primary antibody was detected with labeled anti-rabbit secondary antibody and imaged with Odysey Infrared Imager (Licor).
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bioRxiv - Cell Biology 2022Quote: ... The BiP plasmid was prepared in the pcDNAI vector as previously reported.25 Antibodies were purchased from commercial sources: anti-PDIA4 (Proteintech CAT# 14712-1-AP), anti-KDEL [10C3] (ENZO ...
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bioRxiv - Molecular Biology 2024Quote: ... Taking 4% of precleared chromatin set aside as input and the remainder was added Protein A/G beads pre-bound with specific antibodies against ZNF410 (Proteintech, Cat. # 14529-1-AP), H3K27ac (Abcam ...
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bioRxiv - Microbiology 2024Quote: Immunoblot analysis for the Transferrin content in 2µl volume from each serum was performed with a rabbit polyclonal α-Transferrin IgG (ProteinTech, cat# 17435-1-AP) after standard SDS-PAGE ...
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bioRxiv - Systems Biology 2023Quote: ... Membranes were then washed 3 x 10 min in TBS-T and incubated overnight in primary antibodies against Pfkfb3 (Proteintech Group; 13763-1-AP) and α-tubulin (Cell Signalling Technologies #2125 ...
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bioRxiv - Microbiology 2024Quote: ... the indicated antibodies and dyes were diluted in 5% BSA in PBS as below: mouse anti-Flag (ProteinTech, Cat#66008-4-Ig, 1:5,000), goat-anti-mouse Alexa Fluor-488 (Invitrogen ...
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bioRxiv - Physiology 2024Quote: ... and then each portion was hybridised with an antibody to the protein of interest or beta-ACTIN (Proteintech Group, Inc, China, 20536-1-AP) or GAPDH (Abcam AB8245) ...
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bioRxiv - Cell Biology 2020Quote: ... electrotransferred to nitrocellulose membranes blocked with 5% (w/v) nonfat dry milk and incubated with primary antibodies against CD81 (18250-1-AP, Proteintech Group, Wuhan, P. R. China)(diluted as 1:2500) ...