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Citations for Proteintech :
5001 - 5050 of 5095 citations for Rat TGF beta 1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... The primary antibodies were as follows: anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH; cat. no. 60004-1-lg; Proteintech, Rosemont, IL, USA), mouse monoclonal anti-β-actin (cat ...
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bioRxiv - Biophysics 2022Quote: ... Equal quantities of protein were subjected to 15% SDS-PAGE followed by immunoblotting with anti-SN25 antibody (Proteintech; 14903-1-AP) (Figure 3A) ...
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bioRxiv - Genetics 2022Quote: ... The membrane was blocked for 1 hour with constant rotation in 2.5% Milk-PBS 0.1% tween20 and incubated overnight at 4°C with the EXOSC2 antibody (Proteintech-66099-1-Ig) or GAPDH antibody (Cell Signaling-14C10 ...
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bioRxiv - Microbiology 2021Quote: ... Lysed samples were centrifuged and supernatants were immunoprecipitated with 5µg/ml mouse monoclonal anti-IFITM2/3 antibody (Proteintech, 66081-1-Ig) for 1.5 h at 4°C ...
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bioRxiv - Pathology 2021Quote: ... the membranes were washed and incubated with the corresponding HRP-conjugated secondary anti-mouse and anti-rabbit antibodies (SA00001-2, Proteintech, USA; SA00001-1, Proteintech, USA) for 2 h and then diluted 1:5000 in 5% milk/-PBST ...
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bioRxiv - Cell Biology 2021Quote: ... 800-μg lysates were suspended in 400-μl lysis buffer and immunoprecipitated with 1-μg anti–INPP5E antibody (Proteintech, Rosemont, IL) overnight ...
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bioRxiv - Cancer Biology 2021Quote: Whole cell lysates were made and subjected to SDS-PAGE/western blotting as previously described (26) using the following antibodies: BCL3 (1:2000, Proteintech #, 23959,); CHK2 (1:1000 ...
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bioRxiv - Immunology 2020Quote: ... Immunoblotting was performed according to the antibody manufacturers’ instructions (cGAS: Cell Signaling, 31659; STING: Proteintech, 19851-1-AP; Actin: Fisher, MS1295P1).
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated overnight at 4°C with the primary rabbit anti-ataxin-3 (1:200; ProteinTech, Rosemont, IL, USA), mouse SMI-32 against non-phosphorylated heavy and medium neurofilament subunits (1:1000 ...
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bioRxiv - Cell Biology 2022Quote: ... PVDF membranes were incubated overnight at 4°C with the following primary antibodies: rabbit anti-G-6-PD (1:5000, Proteintech, China), rabbit anti-GLUT-2 (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... they were incubated for 2h in primary antibodies diluted in 5% FCS in PBS at room temperature to stain primary cilia with either rabbit anti-Arl13b (1:500, Proteintech, RRID:AB_2060867) or rabbit anti-Adenylyl cyclase III (ACIII ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was mixed with 0.1 μg/μL RNase A or 1 unit/μL RNasin Plus and incubated with 1 μg of anti-ribosomal protein P0 antibody (isotype: mouse IgG2a κ, clone:9D5) (RN004M, MBL) or isotype control antibody (65208-1-IG, ProteinTech) at 4ºC for 60 min ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were transferred to a nitrocellulose membrane and probed with either a flag tag monoclonal antibody (1:1000; Proteintech, Rosemont, IL) or Alexa Fluor™ 488 streptavidin conjugate (1:3000 ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then incubated overnight at 4°C in rabbit anti-Rab35 (1:100; Cat. 11329-2-AP, Proteintech, Rosemont, IL) or for 1 h at room temperature (RT ...
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bioRxiv - Genetics 2023Quote: ... the membranes were incubated with goat anti-rabbit IgG conjugated with HRP (1:2000 in TBST-5% milk) (SA00001-2, Proteintech, USA) for 90 min at room temperature and developed using western blotting reagents (BL523B ...
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bioRxiv - Bioengineering 2023Quote: ... Quantification of proteins was carried out by densitometry analysis using ImageJ software (v1.54b) and normalised to loading control HRP-Conjugated GAPDH (1:5000; HRP-60004, Proteintech, RRID: AB_2737588).
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bioRxiv - Developmental Biology 2023Quote: ... Immunoprecipitation was performed overnight using 200μl of each lysate (∼chromatin from 10 million cells) and 5µg of antibody (Proteintech 15073-1-AP), following the iDeal ChIP-seq kit for Transcription Factors (Diagenode ...
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bioRxiv - Neuroscience 2023Quote: ... GAPDH-71.1).The following antibodies were used for i3Neuron stable cell lines immunoblot: Rabbit anti-TDP-43 (Proteintech 10782-2-AP 1:1000); Mouse anti-FLAG (Sigma F1804 ...
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bioRxiv - Pathology 2024Quote: ... The membranes were reacted with the following primary antibodies: rabbit anti-TAB1 (1:300, Q15750, polyclonal, rabbit; Proteintech, Rosemont, IL, USA), rabbit anti-IRAK4 (1:300 ...
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bioRxiv - Neuroscience 2024Quote: ... The primary antibody for RBP3 (also known as IRBP) was rabbit anti-RBP3 polyclonal (Proteintech, Catalog No. 14352-1-AP, Illinois). The AdipoR1 RNAScope fluorescent assay was executed using an Automated Leica Bond RX ...
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bioRxiv - Biophysics 2022Quote: ... Membranes were incubated overnight with primary antibodies (1:100 in 5% skim-milk powder, anti-TDP-43 (rb, Proteintech 10782-2-AP), anti-G3BP1 (ms ...
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bioRxiv - Cell Biology 2019Quote: ... The blots were blocked in 4% BSA in TBST solution for 30 min at room temperature and then incubated at 4-°C overnight with the primary antibody: HSP90α(Proteintech, 1:1000), HIF-1α(CST ...
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bioRxiv - Pathology 2019Quote: ... The membranes were incubated for 1hr in blocking buffer (Odyssey) at room temperature before incubation with primary MMP-13 rabbit polyclonal antibody (ProteinTech, 1:333), and monoclonal beta-actin antibody (Abcam ab8226 ...
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bioRxiv - Bioengineering 2021Quote: ... Primary antibodies were diluted in 2% BSA in PBS as recommended by the manufactuerer (mouse anti-human CD9 (Proteintech 60232-1-Ig), mouse anti-human CD63 (Thermofisher MEM-259) ...
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bioRxiv - Neuroscience 2021Quote: ... The membrane was blocked in 4% milk-PBST and proteins were stained using the following antibodies: anti-TDP-43 (rabbit, Proteintech, 1:1000), anti-GAPDH (rabbit ...
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bioRxiv - Physiology 2020Quote: ... The lysates were then centrifuged for 15 min at 16,000 x g and the supernatants were collected for immunoblot analysis with antibodies for TMPRSS2 (Abcam: ab92323 and Proteintech: 14437-1-AP), AR (EMD Millipore ...
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bioRxiv - Cell Biology 2022Quote: ... and equal amounts of protein were incubated with 25 uL RFP-Trap magnetic agarose rotating for 1 hour at 4°C (Proteintech, rta-10). The beads were washed three times and boiled in SDS sample buffer for 10 minutes at 95°C ...
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bioRxiv - Cancer Biology 2022Quote: ... incubated with primary antibodies overnight at 4 °C at dilutions recommended by the suppliers as follows: anti-TSG101 (14497, Proteintech, 1:1,000), anti-CD81 (66866 ...
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bioRxiv - Molecular Biology 2022Quote: ... first with AGAL and lysosome detected as described above and then with subsequent detection of TMED9 with rabbit polyclonal anti-TMED9 antibody (21620-1-AP, Proteintech, Manchester, UK) directly labeled with Daylight 488 Fast Conjugation Kit (Abcam ...
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Loss of the mitochondrial citrate carrier, Slc25a1/CIC disrupts embryogenesis via 2-HydroxyglutaratebioRxiv - Developmental Biology 2024Quote: ... sectioned in the mid sagittal plane and stained with hematoxylin and eosin (H&E) and anti-Slc25a1 antibody (Proteintech, 15235-1-AP), used at 1/150 dilution ...
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bioRxiv - Cell Biology 2024Quote: Antibodies directed against the following proteins were used in this study: SLC25A46 (Santa Cruz; G2-SC-515823 and Proteintech; 12277-1-AP), MFN2 (Cell Signaling ...
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bioRxiv - Neuroscience 2024Quote: ... followed by incubation for 2 hours at RT with primary antibodies in 5% milk in TBS-T at the following dilutions: anti-TDP-43 rabbit polyclonal antibody (1:1500; 10782-2-AP; Proteintech, Rosemont, IL) or anti-Gapdh mouse monoclonal antibody (1:12000 ...
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bioRxiv - Neuroscience 2024Quote: ... cOmplete protease inhibitors) and subsequently incubated overnight at 4°C with 8 µg of either rabbit anti-Mef2c (Proteintech #18290-1-AP) or rabbit IgG isotype control (Abcam #ab27478) ...
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bioRxiv - Cancer Biology 2023Quote: ... Tween-20 PBS buffer) for three times and 100 μl of 1:5000 diluted HRP-conjugated anti-His antibody (Proteintech, HRP-66005) was added into each well and incubated at 4°C overnight or 2 h at RT ...
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bioRxiv - Immunology 2023Quote: ... rabbit N-terminal anti-TDP-43 (#10782-2-AP) and rabbit C-terminal anti-TDP-43 (#12892-1-AP) from Proteintech (Manchester, UK); goat anti-ChAT (#AB144P) ...
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bioRxiv - Molecular Biology 2023Quote: ... Slides were blocked with 3% hydrogen peroxide and 5% horse serum and then incubated with the following primary antibodies: anti-METTL3 (1:100, Proteintech, Wuhan, China), anti-FTO (1:100 ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were blocked with 5% goat serum and then incubated with the following primary antibodies: anti-METTL3 (1:100, Proteintech, Wuhan, China), anti-FTO (1:100 ...
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bioRxiv - Cell Biology 2023Quote: ... and incubated with the following primary antibodies in blocking buffer containing 0.05% Saponin for 2 hours at RT: ARL13B (Proteintech, # 66739, 1:100), γ-tubulin (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... The following antibody was used for HEK-293 cells immunoblot: Rabbit anti-Mouse TDP-43 N-Term (Proteintech 10782-2, 1:2000).
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bioRxiv - Molecular Biology 2023Quote: ... 1:1000 dilution), Anti-β Amyloid (D-11 Santacruz, Cat. Sc-374527, 1:1000) and anti-β-tubulin (Invitrogen Cat.32–2600 or Proteintech Europe ...
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bioRxiv - Pathology 2023Quote: ... membranes were blocked in 5% skimmed milk and proteins detected by primary antibodies overnight at 4℃: anti-αSMA (Proteintech, China,1:20000), anti-MCU (Proteintech ...
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bioRxiv - Molecular Biology 2024Quote: Lysate collection and immunoprecipitation were performed using the Magna RIP immunoprecipitation assay (MilliporeSigma) and 5 μg of rabbit anti-Caprin-1 (Proteintech, Rosemont, IL) or rabbit IgG control ...
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bioRxiv - Biochemistry 2024Quote: ... Membranes were blocked in Intercept PBS Blocking Buffer (Li-Cor Biotechnology, Cambridge, UK) before over-night incubation with anti-RNaseH1 (1:500, Proteintech, Manchester, UK) and anti-Vinculin (1:20,000 ...
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An mRNA processing pathway suppresses metastasis by governing translational control from the nucleusbioRxiv - Cancer Biology 2021Quote: ... Clarified lysates were pooled and RNA-protein complexes were then immunoprecipitated using protein A/G beads (Pierce) conjugated to anti-PABPC4 (Proteintech 14960-1-AP) for 2 hours at 4°C ...
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bioRxiv - Biochemistry 2019Quote: Native RNA immunoprecipitations were performed on one 150 mm dish of HEK293T cells as previously described45 using anti-DDX21 antibody (ProteinTech 15252-1-AP). RT-qPCR analysis was performed on Applied Biosystems StepOnePlus instrument with the RNA to Ct One-step RT-qPCR kit (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2019Quote: ... Efficiency of mRNA depletion was assessed 72 hours post-transfection using qPCR or western blot in the case of ACSL1 (Proteintech, 13989-1-AP). In experiments to determine suppression of cell toxicity resulting from αESA or ML162 by silencing expression of individual ACSL or ALOX genes ...
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bioRxiv - Molecular Biology 2022Quote: ... as a blocking buffer and then overnight at 4°C in the same blocking solution containing one of the following antibodies: anti-HAX1 (rabbit, Proteintech 11266-1-AP), anti-RPL26 (rabbit ...
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bioRxiv - Cell Biology 2022Quote: ... or using Dynabeads-protein G (Thermo) magnetic beads with primary antibodies (anti-GFP Genetex No#GTX113717; anti-Sec5 Proteintech No#12751-1-AP) cross-linked using dimethyl pimelimidate (Life Technologies).
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bioRxiv - Cancer Biology 2020Quote: ... coupled to 1 ml of Protein A agarose resin (Thermo-Fisher: 44893) or with 105 μg of normal rabbit IgG (Proteintech: 30000-0-A) coupled to 1 mL of Protein A agarose resin ...
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bioRxiv - Cancer Biology 2020Quote: ... The supernatant (500 μg of nuclear extract) was incubated for 16 hours at 4°C with 105 μg of PAX8-specific antibody (Proteintech: 10336-1-AP) coupled to 1 ml of Protein A agarose resin (Thermo-Fisher ...