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Citations for Peprotech :
101 - 150 of 1725 citations for Siglec 2 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... Primary human T cells were cultured in complete RPMI 1640 supplemented with 100 U/mL recombinant human IL-2 (PeproTech, 200-02). Cells were cultured at 37°C in a humidified 5% CO2 incubator.
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bioRxiv - Bioengineering 2023Quote: ... Primary human T cells were cultured in complete RPMI 1640 supplemented with 100□U/ml recombinant human IL-2 (PeproTech, 200-02). All mammalian cells were cultured at 37□°C in a humidified 5% CO2 incubator.
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bioRxiv - Microbiology 2020Quote: ... were cocultured with 2×105 peptide-pulsed autologous DCs in a 24-well plate in the presence of 10 ng/mL recombinant human interleukin-2 (IL-2; Peprotech). The next day ...
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bioRxiv - Cancer Biology 2021Quote: ... emerging hematopoietic cells and EBs were cultivated in SFM ((Liu et al. 2015) supplemented with recombinant human 10 ng fiblroblast growth factor type 2 (rhFGF-2, Peprotech), recombinant human 50 ng/mL rhSCF and 30 ng/mL rhIL-3 ...
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bioRxiv - Molecular Biology 2021Quote: ... 2-day cultured activated cells were washed and then cultured for 2 days in culture media supplemented with 20ng/mL recombinant human IL-2 (PeproTech) in the presence or absence of 10μM GC7 (Merck Chemicals) ...
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bioRxiv - Immunology 2024Quote: ... splenocytes were restimulated for 5 hours at 37°C with 1 μg/ml of MHC class I-restricted LCMV peptide gp33-41 in the presence of 2 ng/ml recombinant human IL-2 (Peprotech), 10% fetal bovine serum (premium FBS ...
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bioRxiv - Immunology 2021Quote: ... 100– 200 U/mL recombinant human IL-2 (PeproTech, Rocky Hill, New Jersey, USA), and a final concentration of 12.5% horse serum and 12.5% fetal bovine serum.
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bioRxiv - Genetics 2020Quote: ... Stimulation was performed for 4 days using recombinant human IL-2 (10ng/ml, Peprotech) and Anti-Biotin MACSiBead Particles loaded with CD2 ...
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bioRxiv - Synthetic Biology 2021Quote: ... and 2 ng/mL recombinant human granulocyte macrophage colony-stimulating factor (GM-CSF; PeproTech) unless specified otherwise ...
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bioRxiv - Bioengineering 2021Quote: ... injection and mice were dosed with 1×104 units recombinant human IL-2 (Peprotech) twice daily for 3 days (6 total injections) ...
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bioRxiv - Microbiology 2021Quote: ... Glutamine (2 mM) and supplemented with 100 ng/mL human M-CSF cytokine (Peprotech). Three days post-isolation ...
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bioRxiv - Immunology 2022Quote: ... 1 x glutamax and 50 IU/ml human recombinant IL-2 (Peprotech, Cranbury, NJ). Enriched CD4+ and CD8+ T cells were activated with ImmunoCult NHP CD2/CD3/CD28 T-cell activator (STEMCELL Technologies ...
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bioRxiv - Bioengineering 2024Quote: ... supplemented with 5% FBS (MilliporeSigma) and 50 U/mL of human IL-2 (Peprotech). T cells were activated at 1:1 ratio of cells to dynabeads ...
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bioRxiv - Bioengineering 2024Quote: Analysis of anti-microbial peptide human β-defensin 2 (PeproTech, Cranbury, NJ - 900-K172) was performed in OTMs via ELISA ...
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bioRxiv - Immunology 2023Quote: ... 10 million cells were resuspended in 10 ml medium and stimulated with 25ng/ml recombinant human interleukin-2 (IL-2) (Peprotech #AF200-02). Vγ9+Vδ2+ T cells were kept overnight at 37°C and 5% CO2.
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bioRxiv - Immunology 2019Quote: ... cells were stimulated with recombinant human IL-2 (100 U/ml) (Peprotech, Rocky Hill, NJ) for 45 min at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... and 20 ng/ml recombinant human Fibroblast Growth Factor-basic (FGF-2, 100-18B, Peprotech) onto poly-Lysine D coated cell culture plates (Corning) ...
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bioRxiv - Genomics 2021Quote: ... in a 3:1 beads:cells ratio and 100 U/mL recombinant human IIL-2 (Peprotech). Non-tissue culture treated 12-well plates were prepared by incubating 1 mL PBS + 25 μg/mL Retronectin (Takara ...
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bioRxiv - Genomics 2021Quote: ... in a 3:1 beads:cells ratio and 100 U/mL recombinant human IL-2 (Peprotech).
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bioRxiv - Immunology 2022Quote: ... cells were cultured in complete RPMI containing recombinant human IL-2 (30 U/ml, Peprotech) and stimulated with anti-CD3/anti-CD28 Dynabeads (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... We further supplemented the media with 5 ng/mL recombinant human (rh) IL-2 (Peprotech) and 10 ng/mL rhIL-7 ...
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bioRxiv - Systems Biology 2023Quote: ... and subsequently cultured in complete culture media supplemented with 5 nM human IL-2 (Peprotech) for up to 10 days ...
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bioRxiv - Bioengineering 2022Quote: ... were coated with 2 μg ml-1 of recombinant human PD-L1ECDFc (Peprotech 310-35), ZNRF3ECDFc (R&D systems 7994-RF-025 ...
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bioRxiv - Cell Biology 2023Quote: ... and 2 ng/mL Recombinant Human Transforming Growth Factor-β1 (TGF-β1, PeproTech, 100-21). Cells were passaged when reaching 70% confluency until day 18 ...
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bioRxiv - Cell Biology 2024Quote: ... BMP-2/4) (ERB medium)3 or adding 10 ng/ml human IL-22 (Peprotech) in WENRA4 (Wnt/ R-spondin1 ...
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bioRxiv - Cancer Biology 2024Quote: ... EGM-2 medium supplemented with VEGF (50 ng/ ml, Recombinant Human VEGF, Peprotech 100-20A) was then added in all the inlets and an interstitial flow was created by having different volumes of medium in the inlets (90ul in the inlets of the HUVEC channel and 110ul in the inlets of the opposite channel) ...
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bioRxiv - Biochemistry 2023Quote: ... 40,000 cells were seeded per cavity of a 96-well plate and incubated for 20 h with serial dilutions (1000 – 0.076 pM) of the fusion protein Af2-IL-2 and the reference recombinant human IL-2 (PeproTech, Rocky Hill NJ, USA), respectively ...
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bioRxiv - Genomics 2019Quote: ... recombinant human IFN-γ (specific activity 2×107 U/mg; AF-300-02, Peprotech, London, UK) at 1000 U/ml as described3 ...
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bioRxiv - Neuroscience 2023Quote: ... The final medium was comprised of basal media containing human TGF-β2 (2 ng/mL, Peprotech), murine IL-34 (100 ng/mL ...
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bioRxiv - Immunology 2019Quote: ... 100 IU/mL recombinant human (rh) IL-2 (Proleukin, Novartis) and 10 ng/mL rhIL-15 (Peprotech), and were cultured in a humidified incubator at 37 °C + 5% CO2 ...
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bioRxiv - Developmental Biology 2020Quote: ... These transfected cells were mixed with 2 ml culture medium (20 ng/ml human basic FGF (Peprotech), 1XB27 RA-(Gibco) ...
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bioRxiv - Immunology 2022Quote: ... 14 d IL-2/IL-15 activated human NK cells were plated with K562 leukemia cells at an effector:target (E:T) ratio of 2.5:1 in addition to human IL-2 (100 IU/mL, Peprotech), human IL-15 (20 ng/mL ...
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bioRxiv - Immunology 2019Quote: ... and 100 μm non-essential amino acids) with 100 U/ml of recombinant human IL-2 (PeproTech), which was replaced every 2 days keeping the cells at a concentration of 1.5 × 106 cells/ml ...
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bioRxiv - Immunology 2021Quote: ... in the presence of human IL-2 (30 U/ml) and murine IL-7 (10 ng/ml; PeproTech) in RPMI 1640 media supplemented with 10% heat-inactivated FBS ...
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bioRxiv - Immunology 2021Quote: ... diluted in complete RPMI (cRPMI; RPMI supplemented with 10% FBS plus recombinant human IL-2 (20IU/ml; Peprotech), as previously described.65,66 Activated cells were incubated for six days with a media change on day three.
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bioRxiv - Immunology 2021Quote: ... whole bone marrow was plated at 1M cells/mL in cRPMI + 400U/mL Proleukin human IL-2 (Peprotech). Fresh IL-2 was added every 3rd day ...
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bioRxiv - Immunology 2023Quote: ... and expanded in RPMI 1640 containing 10% FBS and 100 IU/mL of recombinant human IL-2 (Peprotech). Human blood samples were enriched for T cells using negative bead selection (StemCell ...
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bioRxiv - Cell Biology 2021Quote: ... human FGF-basic and human EGF (animal free, Peprotech) at 20 ng/mL ...
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bioRxiv - Biochemistry 2020Quote: ... according to the manufacturer’s instructions and cultured in RPMI supplemented with 10% FBS and 30 U/ml recombinant human IL-2 (PeproTech) at 37°C in 5% CO2 ...
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bioRxiv - Immunology 2021Quote: ... Switzerland) for 11 days in the human plasma serum-containing medium with 3000 IU/ml of IL-2 (PeproTech). The cell cultures were supplemented with fresh media and IL-2 every 2–4 days ...
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bioRxiv - Immunology 2021Quote: ... at a bead to cell ratio of 3:1 and 1,000 U/mL of recombinant human IL-2 (Peprotech). Fresh IL-2 containing media was added every 48 h and Human T-activator CD3/CD28 Dynabeads were added again at day 7 and removed at day 15 of expansion ...
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bioRxiv - Genetics 2022Quote: ... clone 145-2C11, WEHI antibody facility), anti-CD28 (2μg/ml, clone 3751, WEHI antibody facility) and human IL-2 (Peprotech). CD8 T cell cultures contained 25μg/ml anti-mouse IL2 (clone S4B6 ...
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bioRxiv - Immunology 2023Quote: ... in the presence of 2mg/mlaCD28 (clone 37.51, WEHI antibody facility) and 100U/ml recombinant human IL-2 (Peprotech), in 96 well flat-bottom plates at 104 cells per well ...
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bioRxiv - Immunology 2024Quote: CD8 T cells from WT p14 or Irf5-/- p14 mice were isolated as described above and cultured with or without 1µg/ml gp33 peptide in complete RPMI medium supplemented with 2ng/ml recombinant human IL-2 (Peprotech). Cells were then collected at 6 or 24 hours after incubation and washed with PBS ...
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bioRxiv - Immunology 2023Quote: ... at a density of 7.5×105 cells/ml in 2 ml complete media supplemented with 20 ng/ml recombinant human GM-CSF (Peprotech). The plates were incubated at 37°C in 5% CO2 ...
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bioRxiv - Immunology 2020Quote: Human PBMCs were stimulated with recombinant human IL-12 (Peprotech) and IL-18 (R&D Systems ...
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bioRxiv - Cancer Biology 2023Quote: ... human EGF (Peprotech), and FGF (Peprotech) ...
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bioRxiv - Microbiology 2020Quote: ... the PBMCs were cultured with the synthetic peptide mixture of SARS-CoV-2 at a concentration of 1μg/ml or DMSO in RPMI 1640 medium containing 10% FCS and 20 U/ml recombinant human IL-2 (Peprotech) for 7 days ...
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bioRxiv - Cancer Biology 2021Quote: ... 106 anti-CD19 CD8 CAR T cells were expanded in presence of 7 x 106 irradiated (8000 cGy) TM-LCL feeder cells in RPMI1640 containing 50 IU/mL human recombinant IL-2 (Peprotech), 10 ng/ml recombinant human lL-15 (Peprotech) ...
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bioRxiv - Molecular Biology 2022Quote: ... The isolated cells were cultured in RPMI medium supplemented with 20% (v/v) FBS and 100 ng/mL human recombinant IL-2 (Peprotech). Two days prior to transduction ...