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Citations for Peprotech :
551 - 597 of 597 citations for Rat Cell Lysates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... with cells in the third or subsequent passages defined as ‘late hCNCC’ and cultured with added 50 pg/ml BMP2 (Peprotech, 120-02) and 3 uM CHIR 99021 (Selleck ...
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bioRxiv - Cancer Biology 2023Quote: ... 6 ng/ml murine interleukin 3 (mIL3) and 20 ng/ml murine stem cell factor (mSCF) (all from Peprotech, Rocky Hill, NJ), and incubated at 5% CO2 and 37°C ...
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bioRxiv - Immunology 2023Quote: Jurkat and THP1 WT and ARPC5 KO cells were seeded in 96-well plate and stimulated with RPMI 1640 + 100 ng/ml CXCL12 (Peprotech, 300-28A) or RPMI 1640 for the indicated time points ...
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bioRxiv - Immunology 2023Quote: ... 10 million cells were resuspended in 10 ml medium and stimulated with 25ng/ml recombinant human interleukin-2 (IL-2) (Peprotech #AF200-02). Vγ9+Vδ2+ T cells were kept overnight at 37°C and 5% CO2.
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bioRxiv - Cancer Biology 2024Quote: ... according to the manufacturer’s instructions (20 μL of beads per 1 million T-cells) for 5 days and grown in the presence of recombinant interleukin-7 (PeproTech, 200-07) and interleukin-15 (PeproTech ...
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bioRxiv - Immunology 2023Quote: ... TIL or KSQ-001 cells were added to spheroid plate in 100μL REP media supplemented with 6000IU/mL IL-2 (Peprotech, Cat#200-02). Images were taken via Incucyte S3 (Sartorius ...
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bioRxiv - Cancer Biology 2024Quote: ... according to the manufacturer’s instructions (20 μL of beads per 1 million T-cells) for 5 days and grown in the presence of recombinant interleukin-7 (PeproTech, 200-07) and interleukin-15 (PeproTech ...
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bioRxiv - Immunology 2024Quote: ... cells were isolated from bone marrow of C57Bl/6 mice and cultured with granulocyte-macrophage stimulating factor (100ng or 2×103 units per 3×106 cells, Peprotech, Cranbury, NJ) in RPMI-1640 media containing 10% heat inactivated fetal bovine serum ...
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bioRxiv - Biophysics 2021Quote: ... EMT in all cells lines were induced by addition of 5ng/ml Targeted Growth Factor-β1 (TGFβ1) (Peprotech, catalog no. 100-21-10UG) for 48 hours62.
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... RBC-lysed murine bone marrow was seeded at 2 million cells/mL in complete RPMI media with 200 ng/mL human FLT3 ligand (PeproTech, Cat# 300-19) in a 6-well plate (10 million cells/well) ...
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bioRxiv - Immunology 2022Quote: ... The CD34 cell purity was checked by FACS and cells were cultured in IMDM with 10% HI FBS supplemented with human cytokine and growth factor cocktail from Peprotech (hTPO (#300-18) (10 ng/ml) ...
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bioRxiv - Cell Biology 2021Quote: The immature motor neurons were seeded on the cell imaging coverglasses in a low density (1.5×104 cells/cm2) and grown for 2 days in the fourth step medium supplemented with neurotrophic factor (NTF) cocktail: IGF1 (PeproTech, 10 ng/ml), CNTF (PeproTech ...
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bioRxiv - Cell Biology 2021Quote: ... collected in HBSS+/+ and used as controls (3×105 cells/ml) or were incubated with 10 ng/ml TNFα (PeproTech #AF-300-01A), 10 µg/ml recSema7A Fc (R&D 2068-S7) ...
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bioRxiv - Cell Biology 2021Quote: ... cells were treated for 4 days with 50 ng/mL of brain-derived growth factor (BDNF) (Peprotech, Rocky Hill, NJ, USA, #450-02B) in serum-depleted EMEM/F12 media supplemented with 1% pen/strep 88,89.
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bioRxiv - Cancer Biology 2022Quote: ... RRID:CVCL_0161) murine cell lines were grown in RPMI-1640 supplemented with 10% FBS and 10ng/ml murine IL-3 (Peprotech, Cat# 213-13). HEK 293T/17 (ATCC Cat# CRL-11268 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then cultured on untreated plates for 7 days adding 10 ng/ml recombinant murine M-CSF (Peprotech, AF-315-02-100) at day of isolation ...
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bioRxiv - Microbiology 2021Quote: ... control and CSP-stable transfected HepG2 cells were pre-treated with 1 U/mL recombinant human IFN-γ (Peprotech, Rocky Hill, NJ, USA) or equivoluminal medium for 6 h ...
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bioRxiv - Immunology 2019Quote: ... the suspension cells were removed and culture in a new flask along with 10ng/ml recombinant murine IL-3 (Peprotech, Rocky Hill, NJ). Cells were passaged twice per week ...
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bioRxiv - Cancer Biology 2021Quote: ... MCF7 cells were cultured in 2D in the absence or presence of 10 ng/mL human TGFβ (PeproTech®, Rocky Hill, NJ, USA) for 3 weeks where TGFβ was replenished every 2 days ...
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bioRxiv - Cancer Biology 2021Quote: ... or 2) subsequently stimulated with 5 μg/kg T cell chemokine – C-X-C motif chemokine ligand 10 (CXCL10, PeproTech, Rocky Hill, USA) (23) ...
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bioRxiv - Immunology 2020Quote: ... Mouse T cells with the addition of mercaptoethanol and patients’ T cells were incubated in the presence of 10ng/mL of hrIL2 (PeproTech, Rocky Hill, NJ). The identity of all cell lines was evaluated using short tandem repeat profiling performed in-house at the Genomic Core Facility ...
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bioRxiv - Immunology 2021Quote: ... were plated with the irradiated 40LB cells in R10 media with 1 ng/mL recombinant mouse interleukin-4 (rmIL-4; Peprotech, East Windsor, NJ). On day 4 ...
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bioRxiv - Immunology 2022Quote: ... and histone isolation procedures activated CD8+ Teff cells were re-cultured (5 × 106 cells/well in 6-well plates) for up to 48h in IMDM containing 50 U/mL IL-2 (PeproTech, Rocky Hill, NJ) prior to use in assays ...
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bioRxiv - Immunology 2022Quote: ... The following cytokines were added at the indicated concentrations for murine γδT cells: recombinant murine IL-12 (10ng/ml) (Peprotech, Rocky Hill, NJ), IL-15 (50ng/ml ...
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bioRxiv - Immunology 2022Quote: ... bone marrow was isolated from tibiae and femurs and single-cell suspensions were cultured at 10*106 cells per 100 mm bacteriological Falcon petri dish in 10 ml complete T cell medium supplemented with 20 ng/ml GM-CSF (PeproTech, Rocky Hill, NJ). At day 3 ...
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bioRxiv - Immunology 2022Quote: ... or CD11b+ enriched single cell suspensions of B16F10 tumors were cultured in the presence of 50 ng/mL recombinant M-CSF (Peprotech, Cranbury, NJ, USA) in RPMI (Wellgene ...
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bioRxiv - Cancer Biology 2023Quote: ... For cytokine treatment cells were stimulated with either 5 ng/mL of TGFβ1 or 50 ng/mL of BMP6 (both from PeproTech, Rocky Hill, NJ, US). Where indicated ...
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bioRxiv - Microbiology 2023Quote: ... for 48h at a concentration of 1 bead/cell in the presence of 100 U/ml IL-2 (PeproTech, Inc., Cat. #200-02) in RPMI +/+ and were subsequently cultured under hypoxia (HOX - 1% O2 ...
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bioRxiv - Cancer Biology 2023Quote: ... 1.5 x 106 cells/ml were seeded in a 96 well plate and treated with IFN-γ (20ng/ml, Cat#315-05, Peprotech, NJ, USA), IL-21 (20 ng/ml ...
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bioRxiv - Immunology 2023Quote: ... were differentiated from PBMCs by culturing the cells in RPMI complete medium supplemented with 10% AB-positive human serum and 15 ng/ml M-CSF (PeproTech, 300-25-100UG). After 2 days of culturing ...
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bioRxiv - Bioengineering 2024Quote: ... NK cells were allowed to recover at a density of 1E6 cells/mL in antibiotic-free medium containing 100 IU/mL IL-2 (PeproTech, Rocky Hill, NJ) for 72 hours at 37°C ...
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bioRxiv - Immunology 2024Quote: ... Matrices were placed into ImmunoCult™-XF T Cell Expansion Medium (StemCell, Cat: 10981) supplemented with rhIL-2 (200IU; Peprotech, Cat: 200-02), rhIL-7 (10ng/mL ...
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bioRxiv - Immunology 2021Quote: ... cells isolated from the femur and tibia were cultured at 1 × 106 cells/ml in 10 ml of complete DMEM (cDMEM) supplemented with 4% recombinant mouse GM-CSF (Peprotech, Rocky Hill, NJ, USA) at 37°C in 7.5% CO2 ...
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bioRxiv - Bioengineering 2021Quote: PTMs were differentiated from hiPSCs using the above protocol for hiPSC-derived Leydig cells with the modification that PDGF-AA is replaced by human recombinant Platelet-Derived Growth Factor-BB (PDGF-BB, Peprotech, 100-14B, Figure 1B). On day 25 the cells were expanded on PLL-coated plates in DMEM/F12 ...
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bioRxiv - Cancer Biology 2022Quote: Panc1 and 4039 cells grown to 80% confluency over 3 days were stimulated for 30 minutes with 30 ng/mL PDGF (Peprotech Cat. no. 100-00AB), then placed immediately on ice ...
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bioRxiv - Immunology 2022Quote: ... hCD34+ cells were either kept in culture at 37°C in RPMI medium with human recombinant Stem Cell Factor (SCF) at 50 ng/ml (PeproTech, Rocky Hill, NJ, USA), human recombinant thrombopoietin (TPO ...
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bioRxiv - Cancer Biology 2021Quote: Primary mouse or NMR fibroblasts were seeded at 1 × 104 cells/well onto 24-well plates and stimulated with TNF-α (PeproTech; 50 ng/mL), z-VAD-fmk (Abcam ...
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bioRxiv - Neuroscience 2022Quote: ... a small number of spheroids were triturated into a single cell suspension and plated on Matrigel coated glass cover-slips at ~10k cells/cm2 using ASM plus CNTF and BMP4 (Peprotech, 450-13 & 120-05ET). Media was changed every 48 hours ...
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bioRxiv - Neuroscience 2023Quote: ... was added at a final concentration of 3.4 mg/mL to prevent the growth of non-neuronal cells and supplemented with 50 ng/mL murine β-nerve growth factor (PeproTech, Cat# 450-34) with 3 mg/mL DNAse I grade II (Roche ...
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bioRxiv - Cell Biology 2023Quote: ... cells were plated in 1% FBS- containing DMEM for 72 hrs with or without 4ng/mL human recombinant TGF-β (Peprotech; cat #100-21).
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bioRxiv - Molecular Biology 2019Quote: ... After 7 days cells were detached using TryplE and seeded on non-gelatinized bacterial dishes for suspension culture at 3 million cells per 75cm2 and cultured in N2B27 containing 10ng/mL EGF and FGF (Peprotech 315-09 and 100-18B). After 3 days floating aggregates were seeded on gelatinized dishes ...
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bioRxiv - Immunology 2021Quote: ... Sorted cells were seeded in 10 cm tissue-culture dishes of 80 – 90% confluent OP9 cells containing 5 ng/ml Flt-3L (R&D, Catalogue no.-427-FL) and 1 ng/ml IL-7 (PeproTech, Catalogue no.- 217-17-10). For T-cell development assay ...
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bioRxiv - Developmental Biology 2021Quote: ... the SC solution was centrifuged at 300 g for 5 minutes to remove the supernatant and SFM containing 50 μg/ml stem cell factor (PeproTech, 300-07, Cranbury, NJ, USA), 100 μg/ml IL-3 (PeproTech ...
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bioRxiv - Genomics 2022Quote: ... Cell pellets were then resuspended (we used EBs from one plate) in differentiation medium containing 10 ng/ml EGF (Peprotech, Cat. No. AF-100-15) and 10 ng/ml Fgf2 (Peprotech ...
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bioRxiv - Immunology 2023Quote: ... at 1×106 cells/ml in T-175 flasks with 100 ng/ml of either GM-CSF or CSF-1 (PeproTech 300-23 or 300-25). Monocytes were incubated at 37°C/5% CO2 for up to 6 days to differentiate into macrophages ...
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bioRxiv - Cancer Biology 2019Quote: ... The methods of low or high-dose IFN-γ treatment for 6 days in A549 and H460 cells were as follows: recombinant human IFN-γ (Peprotech, Rocky Hill, NJ, USA) were added and cultured for 2 days ...
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bioRxiv - Cancer Biology 2023Quote: ... Activated T-cells were kept in medium supplemented with IL-7 and IL-15 (#200-07 and #200-15, PeproTech, 10 and 5 ng/mL, respectively). Gamma-retroviral transduction was facilitated by RetroNectin (#T100B ...