Labshake search
Citations for Peprotech :
551 - 600 of 5616 citations for PEGSSDA 2 x 0.5 mL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2022Quote: ... cells were transferred to 24-well plates and incubated for three days in medium containing 10 U/mL rhIL-2 (Peprotech) to allow establishment of the knockout ...
-
bioRxiv - Immunology 2022Quote: ... After a one hour resting period cells were transferred to 24-well plates and incubated for three days in medium containing 10U/mL recombinant human IL-2 (Peprotech) to allow establishment of the knockout ...
-
bioRxiv - Immunology 2019Quote: Human CD14+ monocytes were purified from leukocyte preparations purchased from the New York Blood Center and differentiated into blood-derived macrophages for 1–2 d in 10 ng/ml M-CSF (PeproTech) and RPMI 1640 medium (Life Technologies)/10% defined FBS (Hyclone) ...
-
bioRxiv - Cancer Biology 2021Quote: ... and then cultured in CTS T Cell Expansion medium (Thermo) containing 10% FBS and 100 IU/ml human IL-2 (PeproTech). The CellTiter 96 MTS assay (Promega ...
-
bioRxiv - Immunology 2019Quote: ... 50μM 2-ME and 100ng/ml recombinant murine macrophage colony stimulating factor (M-CSF, PeproTech Inc., Rocky Hill, NJ, USA) for 6 days ...
-
bioRxiv - Cancer Biology 2019Quote: ... and maintained in culture for a total of approximately 2-3 months in DMEM supplemented with 1 ng/mL IL-3 (Peprotech). Mycoplasma testing was not performed ...
-
bioRxiv - Immunology 2021Quote: ... Forty-eight hours later cells were lifted off the plates and cultures were supplemented with 100 U/ml recombinant human IL-2 (Peprotech). CFSE labeling for certain experiments was performed prior to seeding on tissue culture plates.
-
Neutralizing activity of broadly neutralizing anti-HIV-1 antibodies against primary African isolatesbioRxiv - Immunology 2020Quote: ... A total of 2×106 CD4+ T lymphocytes were activated using anti CD3/CD2/CD28 beads (Miltenyi) and cultured in the presence of 100 U/mL IL-2 (Peprotech) at 37 °C and 5% CO2 ...
-
bioRxiv - Cancer Biology 2022Quote: ... Quiescence was induced by plating cells at a density of 10 cells/mm2 in NSC media in the absence of EGF/FGF-2 and supplemented with BMP4 (10 ng/ml, Peprotech). Cells were treated for 1 day or 3 days ...
-
bioRxiv - Bioengineering 2023Quote: ... After gelation samples were submerged in IMR90 medium with/without 2 ng/mL TGF-β2 (100-35B; PeproTech, Cranbury, NJ). Cultures were monitored for loss of collagen adhesion and contraction of the gel for 21 d and survival was quantified as described above.
-
bioRxiv - Immunology 2023Quote: ... splenocytes were labelled with cell trace violet (CTV) (ThermoFischer Scientific) and cultured for 48 h with 10 ng/ml rhIL-2 (Peprotech) in vitro ...
-
bioRxiv - Cancer Biology 2023Quote: ... HSCL or fetal liver LSK cells were cultured for 2 weeks on OP9 or OP9-DL4 cell layer in HSCL medium supplemented with 10 ng/mL Flt-3 (PeproTech) and 10 ng/mL IL-7 (PeproTech).
-
bioRxiv - Immunology 2023Quote: ... mouse bone marrow was flushed and cultured for 2 days in stem cell medium (DMEM + 15% FCS + 25ng/ml SCF (PeproTech) + 10ng/ml IL-3 (PeproTech ...
-
bioRxiv - Neuroscience 2023Quote: ... Then NPCs were seeded on Matrigel®-coated flasks at 50 000 cells/cm2 and kept in Astrocyte Induction medium (DMEM/F-12 supplemented with 1x N2 supplement, 1x B27 supplement, 10ng/ml FGF-2 (PeproTech) and 10ng/ml EGF (Miltenyi) ...
-
bioRxiv - Immunology 2023Quote: ... cells were washed and then expanded and differentiated in complete IMDM (as above) containing 20 ng/mL recombinant human IL-2 (Peprotech) for a further 4 days ...
-
bioRxiv - Immunology 2023Quote: ... The cells were washed and replated in complete RPMI 1640 medium with 100 U/ml IL-2 (PeproTech, 200-02) 48 hours later ...
-
bioRxiv - Immunology 2024Quote: ... cells were thawed and rested in an 37°C / 5% CO2 incubator in the T cell medium with 50 U/mL rhIL-2 (Peprotech, Thermo Fisher Scientific ...
-
bioRxiv - Bioengineering 2024Quote: ... CD8 T cells were transferred to a new 96-well round bottom plate to remove activation and continued to be cultured in complete RPMI supplemented with a higher concentration of IL-2 (10ng/ml; Peprotech).
-
bioRxiv - Neuroscience 2024Quote: ... the medium was changed to NDM supplemented with 20 ng/ml fibroblast growth factor-2 (FGF2; Peprotech, Westlake Village, CA). Media was changed daily ...
-
bioRxiv - Cell Biology 2020Quote: ... 500 μg BMP-2 (rhBMP-2, Peprotech, Hamburg, Germany) was dissolved in 1 mL of ultrapure water to form BMP-2 solution (500 μg/mL) ...
-
bioRxiv - Developmental Biology 2020Quote: ... DFs cultured in DMEM/F12 with 2% FBS supplemented with TGFβ1 or TGFβ3 (10 ng/ml; PeproTech EC, Ltd., London, UK) or 5 mM citric acid (diluent for lyophilized TGFNβ ...
-
bioRxiv - Immunology 2019Quote: ... and maintained in HIPP™-T009 medium (Bioengine) in the presence of 2% autoserum and IL-21 (30 ng/ml, PeproTech) in a 37 °C 5% CO2 incubator for 12 days ...
-
bioRxiv - Bioengineering 2021Quote: ... and re-plated for 2 additional passages at a density of 5 × 103 cells/cm2 at 5% pO2 in expansion medium supplemented with 5 ng/ml of fibroblast growth factor (FGF)-2 (PeproTech Ltd). Medium change was performed three times per week.
-
bioRxiv - Developmental Biology 2019Quote: ... Cells were then washed 2 times in PBS and replaced with medium containing fresh SB431542 or 5ng/mL TGFβ1 (Peprotech, 100-21C) or 10ng/mL Activin A (Sigma Aldrich ...
-
bioRxiv - Cancer Biology 2019Quote: ... Cultures were maintained in complete RPMI with 200-300 U/ml human recombinant IL-2 (PeproTech, Rocky Hill, NJ #200-02) and media was refreshed every 3 days ...
-
bioRxiv - Cancer Biology 2022Quote: ... PBMCs were harvested from K3EDTA anti-coagulated whole blood by density gradient centrifugation with Lymphoprep and plated in 12 well plates at 2-4 × 106 cells per well in 10% RPMI + 10 ng/mL human CSF (300-25, Peprotech, UK). Medium was replaced every 2-3 days until adherent populations of macrophages had established ...
-
bioRxiv - Cell Biology 2023Quote: ... at a density of 0.15×106 cell/well and cultured for 2 days in supplemented α-MEM with 30 ng/ml M-CSF (PeproTech, UK). Media were replaced with α-MEM supplemented with 30 ng/ml M-CSF and 30 ng/ml RANKL (PeproTech ...
-
bioRxiv - Immunology 2024Quote: ... Transduced cells were incubated for 4 days with plate-bound anti-CD3/anti-CD28 in media supplemented with hIL-2 (0.0344 units/mL, Peprotech #200-02). Transduction efficiency was assessed for each experiment by flow cytometry.
-
bioRxiv - Cell Biology 2021Quote: ... mice were injected intraperitoneally with 0.5 μg IL-1β (Peprotech, 211-11B) in 100 μl PBS/0.2% BSA or vehicle (PBS/0.2% BSA ...
-
bioRxiv - Immunology 2021Quote: ... MD-2 (PeproTech) recombinant protein and anti-CD14 antibody (Beckman ...
-
bioRxiv - Neuroscience 2022Quote: ... EGF-2 (PeproTech), FGF (PeproTech ...
-
bioRxiv - Immunology 2020Quote: ... MD-2 (PeproTech) recombinant protein and CD14 antibody (Beckman ...
-
bioRxiv - Developmental Biology 2020Quote: ... the culture medium was replaced with fresh DMEM/F12 with 2% FBS supplemented with TGFβ1/TGFβ3 (10 ng/ml; PeproTech EC, Ltd., London, UK) or 5mM citric acid (diluent for lyophilized TGFβ ...
-
bioRxiv - Cell Biology 2019Quote: ... medium is made with DMEM/F12 together with the following components: 100 ng/mL Fetal Growth Factor-2 (FGF2 Peprotech #450-33), 100 ng/mL Epidermal Growth factor (EGF ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were deprived of serum for 2h and stimulated with 2 ng/ml of recombinant human EGF (Peprotech; Catalog #AF-100-15) for 2 ...
-
bioRxiv - Bioengineering 2020Quote: ... Primary human T cells were cultured in complete RPMI 1640 supplemented with 100 U/mL recombinant human IL-2 (PeproTech, 200-02). Cells were cultured at 37°C in a humidified 5% CO2 incubator.
-
bioRxiv - Systems Biology 2020Quote: ... 2 mM glutamine and 10% fetal calf serum in the presence of 50 ng/ml of M-CSF (Peprotech, cat# 300-25). 5 × 105 CD14+CD16− monocytes or × 105 resting macrophages were activated with 10 ng/ml of lipopolysaccharides (LPS ...
-
bioRxiv - Immunology 2021Quote: ... cells were starved for 6 hours in fresh media lacking cytokines and subsequently treated with 100 U/mL of either IL-2 or IL-15 (PeproTech 200-15).
-
bioRxiv - Microbiology 2020Quote: ... Frozen activated PBMC cells were thawed and resuspended in complete PBMC media containing RPMI supplemented with 21 ng/mL human IL-2 (Peprotech; #200-02), 10% fetal bovine serum and 1% penicillin-streptomycin ...
-
bioRxiv - Microbiology 2020Quote: ... Pellets were resuspended in activation medium containing RPMI supplemented with 21 ng/mL human IL-2 (Peprotech, Rocky Hill, NJ; #200-02), 1.5 μg/mL PHA (Remel ...
-
bioRxiv - Immunology 2020Quote: ... cells were washed and cultured for a further 4 days in media containing 20 ng/mL recombinant human IL-2 (Peprotech, London, UK). For analysis of effector cytokine production ...
-
bioRxiv - Developmental Biology 2022Quote: ... The cells were cultured on pre-coated plates with 0.5% growth factor-reduced Matrigel at a density of 2×104 cells/cm2 with MEF-conditioned medium supplemented with 10 ng/ml BMP4 (Peprotech, 120-05ET), 1µM A83-01 ...
-
bioRxiv - Systems Biology 2020Quote: ... on Day 1 we changed the medium to RPMI + 2% B-27 (minus insulin) + 5ng/ml BMP4 (Peprotech, cat. AF-120-05ET) + 1uM Chiron 99021 and incubated these cells for 48 hours ...
-
bioRxiv - Bioengineering 2019Quote: ... 1 ng/mL human fiberblast growth factor-2 (hFGF2) and 0.25 ng/mL transforming growth factor-β1 (TGF-β1; all growth factors from PeproTech, Rocky Hill, NJ). Cells were treated with fresh medium every two days.
-
bioRxiv - Immunology 2019Quote: ... 50μM 2-ME and 10 ng/ml of recombinant murine granulocyte– macrophage colonystimulating factor (GM-CSF, PeproTech Inc., Rocky Hill, NJ, USA) for 7 days ...
-
bioRxiv - Immunology 2022Quote: ... same number of magnetically sorted naive T cells were co-cultured with equal numbers of BMDCs supplemented with 20 ng/ml of recombinant mouse IL-2 (PEPROTECH: 212-12) for 24-72 hours ...
-
bioRxiv - Systems Biology 2023Quote: B2B1 cells were seeded on tissue culture plastic (6-well to 15-cm plates) at 12,500 cells/cm2 in assay medium + 2% matrigel + 5 ng/ml EGF (Peprotech, AF-100-15) and refed every four days ...
-
bioRxiv - Immunology 2023Quote: ... 55 uM 2-mercaptoethanol and 20 ng/ml of recombinant murine granulocyte-macrophage colony-stimulating factor (PeproTech, Rocky Hill, NJ, United States)] at 1:5 apoptotic cancer cell to cDC1 ratio ...
-
bioRxiv - Bioengineering 2023Quote: ... Primary human T cells were cultured in complete RPMI 1640 supplemented with 100□U/ml recombinant human IL-2 (PeproTech, 200-02). All mammalian cells were cultured at 37□°C in a humidified 5% CO2 incubator.
-
bioRxiv - Developmental Biology 2023Quote: ... cells were washed with PBS and further cultured for two days in S1/2 medium supplemented with FGF7 (50ng/ml) (PeproTech, 100-19) and vitamin C (0.25mM ...