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Citations for Peprotech :
551 - 600 of 663 citations for Neural Precursor Cell Expressed Developmentally Down Regulated 9 NEDD9 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: ... the digested cells were first incubated in RPMI 1640 with 10% FBS and 10 ng/ml recombinant murine IL-7 (PeproTech), and stimulated with 50 ng/ml phorbol 12-myristate 13-acetate ...
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bioRxiv - Cancer Biology 2024Quote: ... at 3:1 bead: T-cell ratio together with human recombinant IL-15 and IL-7 (PeproTech; 5 ng/mL) in AIM-V medium supplemented with 5% FBS ...
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bioRxiv - Cell Biology 2024Quote: ... and cultivated in 4 mL of B-cell medium containing either 10 ng·mL−1 rIL-4 or 10 ng·mL−1 rIL-21 (Peprotech, 210-21). On day 8 ...
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bioRxiv - Immunology 2024Quote: ... CD14+ monocytes were isolated from PBMCs using the EasySep Human CD14 Positive Selection Kit (Stem Cell) and cultured with 100U/mL GM-CSF (Peprotech) for 4 days prior to plating for stimulation ...
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bioRxiv - Immunology 2024Quote: ... cells were thawed and rested in an 37°C / 5% CO2 incubator in the T cell medium with 50 U/mL rhIL-2 (Peprotech, Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... Hepatocyte-like cells were made by further incubating the cultures in Stage 3a media (HCM containing 20ng/mL Hepatocyte Growth Factor (Peprotech)) for 5 days followed by a further 15 days in Stage 2b media (HCM containing 10 𝜇g/mL Oncostatin M (R&D Systems Cat No ...
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bioRxiv - Developmental Biology 2024Quote: ... cells were replated as single cells onto poly-D-lysine-laminin-coated plates at 1×106 cells/cm2 for astrogenic switch and progenitor expansion in N2B27 media supplemented with 10 ng/mL FGF2 (Peprotech) and 10 ng/mL Human EGF (Peprotech ...
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bioRxiv - Immunology 2024Quote: ... Approximately 5 × 106 NK cells were co-cultured with 1 × 107 of the modified and irradiated K562 feeder cells in 40 mL of Complete NK MACS medium supplemented with 100 U/mL of recombinant IL-2 (Peprotech) in a T75 flask (Corning) ...
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bioRxiv - Biophysics 2021Quote: ... bone marrow progenitor cells were cultured in complete RPMI media supplemented with interleukin-4 (IL-4, 10 ng/mL, PeproTech, Canada) and granulocyte-macrophage colony-stimulating factor (GM-CSF ...
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bioRxiv - Developmental Biology 2021Quote: ... and treated with 25 ng/ml recombinant (r) human TGFβ1 derived from HEK293 cells (100-21, PeproTech, Rocky Hill, NJ, USA) for 24 hours.
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bioRxiv - Cell Biology 2022Quote: ... Cells were treated with either low-serum media or low-serum media supplemented with 20 ng/mL HGF (PeproTech, 100-39) or by standard culture media supplemented with Lomustine (Selleckchem ...
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bioRxiv - Neuroscience 2021Quote: ... NSCs were detached as described for cell maintenance and seeded at a density of 100,000 cells into precoated 24 wells in N2B27 medium supplemented with 20 ng/ml BMP4 (Peprotech, 315-27). After 3 days of differentiation in BMP4-supplemented N2B27 medium ...
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bioRxiv - Neuroscience 2021Quote: ... cells were detached with 0.05 % trypsin and re-plated on 0.1 % gelatin-coated flasks as single cells in N2B27 medium supplemented with 20 ng/ml FGF (Peprotech, 450-33) and 20 ng/ml EGF (Peprotech ...
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bioRxiv - Immunology 2021Quote: Both Lin-CD25+CD44- DN3 cells and Lin-CD25-CD44- DN4 cells were sorted from the thymi of LckCre+Zfp335+/+ and LckCre+Zfp335fl/fl mice and co-cultured with OP9-DL1 feeder cells in α-MEM medium in the presence of IL-7 (1 ng/ml, PeproTech) and Flt3-L (5 ng/ml ...
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bioRxiv - Bioengineering 2021Quote: ... and re-plated for 2 additional passages at a density of 5 × 103 cells/cm2 at 5% pO2 in expansion medium supplemented with 5 ng/ml of fibroblast growth factor (FGF)-2 (PeproTech Ltd). Medium change was performed three times per week.
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bioRxiv - Cell Biology 2021Quote: ... PMNs (2.5×106 cells/ml) were used as a control or stimulated with 10 ng/ml TNFα (PeproTech #AF-300-01A) for 15 min at 37°C ...
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bioRxiv - Neuroscience 2019Quote: ... The concentration of TNF in cell culture supernatants was quantified by enzyme-linked immunosorbent assay (ELISA) following the instructions provided by the manufacturer (Peprotech, UK). Absorbance at 405 nm ...
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bioRxiv - Cancer Biology 2020Quote: Cells were seeded in 96-well plates in 100 µl media per well at a density of 40,000 cells/ml one day prior to treatment with soluble recombinant TRAIL (rTRAIL; Peprotech-310-04) or MEDI3039 (Medimmune) ...
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bioRxiv - Biochemistry 2022Quote: ... purified cells were cultured in complete medium added with 100 ng/ml macrophage colony-stimulating factor (M-CSF) (Peprotech, Stockholm, Sweden) or 100 ng/ml granulocyte–macrophage colony-stimulating factor (GM-CSF ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: HBD1 and LL-37 peptide release was quantified in supernatants from 25 day long-term cultured A549 cells using a human BD-1 Standard ABTS ELISA Development Kit (PeproTech, Germany) and LL-37 human ELISA kit (HycultBiotech ...
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bioRxiv - Biochemistry 2019Quote: ... The cells were subject to cytokines stimulation in serum free medium containing 10 ng/ml rat IFN-γ (Peprotech, #400-20) and 10 ng/ml rat TNF-α (Peprotech ...
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Combination image flow cytometry reveals novel methods for isolating megakaryocyte progenitors cellsbioRxiv - Cell Biology 2019Quote: ... 1×107 cells were incubated in IMDM + 10% FBS supplemented with 50 ng/ml of recombinant murine TPO (PeproTech, #315-14) in a T75 flask at 37 °C in 5% CO2 for 72 h.
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bioRxiv - Immunology 2019Quote: ... Lin- bone marrow cells were grown in culture with complete IMDM medium supplemented with 10 ng/ml M-CSF (Peprotech, QC).
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bioRxiv - Immunology 2021Quote: ... then the remaining negatively sorted cells were resuspended at 5×105 cells/ml in complete Iscove’s modified Dulbecco’ s medium supplemented with Granulocyte-macrophage colony-stimulating factor (GM-CSF) (PeproTech, #315-03), FLT-3L (PeproTech ...
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bioRxiv - Immunology 2020Quote: PBMCs were plated in 24-well plates at 2×106 cells per well with 100 IU/ml IL-2 (PeproTech, USA) in the complete medium ...
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bioRxiv - Neuroscience 2022Quote: ... then 4 million cells were seeded per well in 2 ml of EB Medium: complete mTeSR + 0.05 ug/ml BMP-4 (PeproTech, #120-05) + 0.05 ug/ml VEGF (PeproTech ...
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bioRxiv - Cell Biology 2022Quote: ... Activated cells were incubated for 5 days in the presence of the following: IL-12 (25 ng/ml, PeproTech, London, UK) and neutralizing antibody anti–IL-4 (1 μg/ml ...
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bioRxiv - Neuroscience 2022Quote: ... 20 ng/ml glial cell line-derived neurotrophic factor and 20 ng/ml brain-derived neurotrophic factor (Peprotech, East Windsor NJ). Cells were plated on polyornithine/laminin-coated wells ...
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bioRxiv - Cancer Biology 2022Quote: ... PBMCs were harvested from K3EDTA anti-coagulated whole blood by density gradient centrifugation with Lymphoprep and plated in 12 well plates at 2-4 × 106 cells per well in 10% RPMI + 10 ng/mL human CSF (300-25, Peprotech, UK). Medium was replaced every 2-3 days until adherent populations of macrophages had established ...
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bioRxiv - Cell Biology 2023Quote: ... at a density of 0.15×106 cell/well and cultured for 2 days in supplemented α-MEM with 30 ng/ml M-CSF (PeproTech, UK). Media were replaced with α-MEM supplemented with 30 ng/ml M-CSF and 30 ng/ml RANKL (PeproTech ...
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bioRxiv - Immunology 2023Quote: ... ROSAKIT WT cells were seeded at 0.3 x 106 cells/ml and treated for 4 days with IL-4 (20 ng/ml, PeproTech, #200-04) and human IgE (1 µg/ml ...
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bioRxiv - Immunology 2023Quote: EndoC-βH1 cells and dispersed human islets were exposed or not to IFNα (2000 U/mL, PeproTech Inc., Rocky Hill, NJ), IFNγ (1000 U/mL ...
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The loss-of-function of SOCS2 increases the inflammatory response to Staphylococcus aureus infectionbioRxiv - Immunology 2023Quote: ... The cells were collected and cultured in X-Vivo (BE02-060F, Ozyme, France) supplemented with 50 ng/mL M-CSF (Peprotech, France) at a density of 2 x105 cells/mL ...
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bioRxiv - Immunology 2023Quote: ... Transfected cells were incubated for 24 hours before treatment with 50 ng/mL recombinant human IFN-γ (Peprotech; cat # 300-02) or cDMEM only control for 24 hours ...
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bioRxiv - Neuroscience 2023Quote: ... and plated on poly-L-ornithine/laminin (PLO)-coated dishes at a density of 10,000–15,000 cells/cm2 in N2B27 medium supplemented with 100 ng/ml FGF-8 (PeproTech, Cat.100-25), 200 ng/ml sonic hedgehog (PeproTech ...
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bioRxiv - Bioengineering 2023Quote: ... cells were differentiated at a concentration of 5 x 105 cells/mL with 100 nM phorbol 12-myristate 13-acetate (PMA, PeproTech, 1652981) in complete RPMI media for 24 hours followed by 3 days of resting in complete RPMI media ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated for the indicated time in the absence or presence of IL-7 (10 ng/ml, Peprotech, Cat#: 200-07) or stem cell factor (20 ng/ml ...
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bioRxiv - Cell Biology 2022Quote: ... The cell suspension was washed with PBS and plated in DMEM with 10 ng mL-1 M-CSF (Peprotech Inc, 315-02). Following 5 days of culture ...
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bioRxiv - Biochemistry 2019Quote: ... at 1 M cells per well in unconditioned medium with or without priming in 25 U IFN-γ (PeproTech, Rocky Hill, NJ). Bone-marrow-derived mast cells were prepared as described previously (55) ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were deprived of serum for 2h and stimulated with 2 ng/ml of recombinant human EGF (Peprotech; Catalog #AF-100-15) for 2 ...
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bioRxiv - Bioengineering 2020Quote: ... Primary human T cells were cultured in complete RPMI 1640 supplemented with 100 U/mL recombinant human IL-2 (PeproTech, 200-02). Cells were cultured at 37°C in a humidified 5% CO2 incubator.
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bioRxiv - Cell Biology 2020Quote: ... cells were plated in 10 cm diameter Petri dishes with 10 ng/mL macrophage colony-stimulating factor (M-CSF, PeproTech, London, UK) for 24 h ...
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bioRxiv - Microbiology 2020Quote: ... Frozen activated PBMC cells were thawed and resuspended in complete PBMC media containing RPMI supplemented with 21 ng/mL human IL-2 (Peprotech; #200-02), 10% fetal bovine serum and 1% penicillin-streptomycin ...
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bioRxiv - Cancer Biology 2020Quote: ... 6 ng/ml murine Interleukin 3 (mIL3) and 20 ng/ml murine stem cell factor (mSCF) (all from Peprotech, Rocky Hill, NJ), and incubated in 5% CO2 at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... bone marrow progenitor cells were collected in cRPMI and replated in cRPMI containing 20 ng/mL recombinant murine GM-CSF (Peprotech, 315-03) (DC media) ...
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bioRxiv - Immunology 2022Quote: ... Cells were transdifferentiation for 7 days at 60,000 cells/cm2 in medium containing 50 ng/ml IL-3 (200-03 B, Peprotech, New Jersey, USA), 50 ng/ml M-CSF (300-25 B ...
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bioRxiv - Cancer Biology 2022Quote: Single-cell suspensions of cell lines were suspended at a density of 2,000 cells/ml in DMEM/Ham’s F-12 (nacalai tesque, no. 11581-15) containing 20 ng/ml bFGF (Peprotech, no. 100-18B), 20 ng/ml EGF (Wako ...
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bioRxiv - Developmental Biology 2022Quote: ... The cells were cultured on pre-coated plates with 0.5% growth factor-reduced Matrigel at a density of 2×104 cells/cm2 with MEF-conditioned medium supplemented with 10 ng/ml BMP4 (Peprotech, 120-05ET), 1µM A83-01 ...
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A critical role for heme synthesis and succinate in the regulation of pluripotent states transitionsbioRxiv - Developmental Biology 2022Quote: ... 33010-018)-coated plates at a density of 30 000 cells/cm2 and by supplementing the N2B27 medium with 12 ng/ml of bFGF (Peprotech, 100-18B) and 20 ng/ml of activin A (Peprotech ...
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bioRxiv - Immunology 2019Quote: ... Samples were adjusted to 7.5×105 cells/mL with cold cRPMI_M supplemented with 25 ng/mL of recombinant mouse colony-stimulating factor (m-csf, Peprotech, Rocky Hill, NJ) and cultured at 37°C ...