Labshake search
Citations for Peprotech :
251 - 300 of 2243 citations for IL 7 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2022Quote: ... 1 x glutamax and 50 IU/ml human recombinant IL-2 (Peprotech, Cranbury, NJ). Enriched CD4+ and CD8+ T cells were activated with ImmunoCult NHP CD2/CD3/CD28 T-cell activator (STEMCELL Technologies ...
-
bioRxiv - Genomics 2023Quote: ... and 15 ng/mL of recombinant human interleukin 2 (IL-2) (Peprotech, 200-02). Cells were kept in a humidified 5% CO2 atmosphere at 37°C.
-
bioRxiv - Immunology 2024Quote: ... The complex was freshly prepared by incubating recombinant human IL-15 (Peprotech, 200-15) with IL-15Rα-Fc (R&D Systems ...
-
bioRxiv - Bioengineering 2024Quote: ... supplemented with 5% FBS (MilliporeSigma) and 50 U/mL of human IL-2 (Peprotech). T cells were activated at 1:1 ratio of cells to dynabeads ...
-
bioRxiv - Biochemistry 2023Quote: ... 40,000 cells were seeded per cavity of a 96-well plate and incubated for 20 h with serial dilutions (1000 – 0.076 pM) of the fusion protein Af2-IL-2 and the reference recombinant human IL-2 (PeproTech, Rocky Hill NJ, USA), respectively ...
-
bioRxiv - Cancer Biology 2023Quote: ... Measurement of IL-6 levels was performed by sandwich ELISA using the human IL-6 mini ABTS ELISA development kit (Peprotech-catalog no 900-M16).
-
bioRxiv - Immunology 2020Quote: 250 000 sorted DN2 and DN3 thymocytes from either IL-7rα+/+ or IL-7rα+/- mice were cultured in 96 well plates for 24 hours onto OP9-Dll4 stromal cells with 100μl of supplemented IMDM medium containing 5ng/ml murine IL-7 and 5ng/ml murine Flt3-Ligand (Peprotech). The medium was stored at -80°C until analysis ...
-
bioRxiv - Immunology 2020Quote: ... The homeostatic stimuli were provided by 2.104 DC purified from autologous C57BL/6 (H-2b) mice and IL-7 (20 ng/ml, Peprotech). Each 4-5 days of culture ...
-
bioRxiv - Synthetic Biology 2020Quote: ... cells were spun down and supernatants were collected and analyzed by ELISA for IL-7 following the manufacturer’s recommended protocol (Peprotech).
-
bioRxiv - Cancer Biology 2022Quote: ... 1 µM of each synthetic peptide (>90% purity, DGPeptidesCo Ltd.) was added to the culture along with 0.5 ng/ml Interleukin (IL)-7 (Peprotech), 50 ng/ml Tumor necrosis factor (TNF)-α (Peprotech ...
-
bioRxiv - Immunology 2022Quote: ... followed by addition of 100,000 sorted CD5- or CD71- DN3 thymocytes per well together with 10 ng/ml recombinant mouse IL-7 (PeproTech).
-
bioRxiv - Immunology 2024Quote: ... T cells were removed from stimulation and cultured in complete medium supplemented with10ng/ml recombinant mouse IL-7 (Peprotech) for 3-5 days as described (69).
-
bioRxiv - Bioengineering 2020Quote: ... Primary human T cells were cultured in complete RPMI 1640 supplemented with 100 U/mL recombinant human IL-2 (PeproTech, 200-02). Cells were cultured at 37°C in a humidified 5% CO2 incubator.
-
bioRxiv - Microbiology 2019Quote: Concentration of IL-8 and CXCL16 were measured in the simplified inflammation model with the Human IL-8 (CXCL-8) and Human CXCL16 Mini ABTS ELISA Development Tests (PeproTech, London, UK) and the ABTS ELISA Buffer Kit (PeproTech ...
-
bioRxiv - Bioengineering 2023Quote: ... Primary human T cells were cultured in complete RPMI 1640 supplemented with 100□U/ml recombinant human IL-2 (PeproTech, 200-02). All mammalian cells were cultured at 37□°C in a humidified 5% CO2 incubator.
-
bioRxiv - Immunology 2019Quote: ... cells were stimulated with recombinant human IL-2 (100 U/ml) (Peprotech, Rocky Hill, NJ) for 45 min at 37°C ...
-
bioRxiv - Microbiology 2020Quote: ... and a Human IL-8 Standard TMB ELISA Development Kit (PeproTech, catalog number 900-T18), according to manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2021Quote: ... murine IL-3 (213-13) and Recombinant Human EPO (100-64) were obtained from PeproTech.
-
bioRxiv - Microbiology 2020Quote: Dividing THP-1 cells were treated with 10 ng/mL recombinant human IL-1β (Peprotech) for the times indicated ...
-
bioRxiv - Genomics 2021Quote: ... in a 3:1 beads:cells ratio and 100 U/mL recombinant human IL-2 (Peprotech).
-
bioRxiv - Immunology 2020Quote: ... M2c cells with 25 ng/mL recombinant human IL-10 (PeproTech; Rock Hill, NJ, USA); and M2d MΦs from stimulation with and 50 ng/mL recombinant human IL-6 (R&D Systems ...
-
bioRxiv - Immunology 2022Quote: ... cells were cultured in complete RPMI containing recombinant human IL-2 (30 U/ml, Peprotech) and stimulated with anti-CD3/anti-CD28 Dynabeads (Invitrogen ...
-
bioRxiv - Immunology 2022Quote: ... Monocytes were cultured in cRPMI supplemented with recombinant human IL-4 and GM-CSF (Peprotech) to generate immature monocyte-derived dendritic cells (Mo-DCs) ...
-
bioRxiv - Immunology 2022Quote: ... in the absence or presence of human recombinant (hr) IL-1β (10 ng/mL, Peprotech) and hrIL-23 (20 ng/mL ...
-
bioRxiv - Immunology 2022Quote: ... We further supplemented the media with 5 ng/mL recombinant human (rh) IL-2 (Peprotech) and 10 ng/mL rhIL-7 ...
-
bioRxiv - Systems Biology 2023Quote: ... and subsequently cultured in complete culture media supplemented with 5 nM human IL-2 (Peprotech) for up to 10 days ...
-
bioRxiv - Physiology 2023Quote: ... Recombinant Human TNF-α (300-01A) and IL-1β (200-01B) were acquired from PeproTech. DAPI (4’,6-diamidino-2-phenylindole ...
-
bioRxiv - Cell Biology 2024Quote: ... BMP-2/4) (ERB medium)3 or adding 10 ng/ml human IL-22 (Peprotech) in WENRA4 (Wnt/ R-spondin1 ...
-
bioRxiv - Immunology 2023Quote: ... purified monocytes were treated for 7 days with 50 ng/ml human recombinant M-CSF (PeproTech) in RPMI medium supplemented with 10% heat inactivated fetal bovine serum ...
-
bioRxiv - Immunology 2021Quote: ... with 2% FBS (HyClone Thermo Fischer Scientific) for 15 minutes with or without murine IL-7 (50 ng/mL, PeproTech). Following stimuli ...
-
bioRxiv - Systems Biology 2019Quote: ... All in vitro T cell generation cultures were supplemented with 5ng/mL Flt3-L and 5 ng/mL IL-7 (Peprotech), and were sorted after 6 or 7 total days of culture before transducing with retroviral vectors or treating with small molecule inhibitors ...
-
bioRxiv - Immunology 2022Quote: ... OT-I T cells were maintained inactivated in vitro (‘No TCR’ condition) with recombinant mouse IL-7 (Peprotech, 10ng/ml). For IL-2 replenishment experiments ...
-
bioRxiv - Immunology 2022Quote: ... The culture conditions used in this study were as follows: HSP condition – cell cultured with IL-15 and -7 (10 μg/ml, PeproTech); HSP+TCR condition – cells cultured with IL-15 and -7 and activated by anti-CD3 (5 μg/ml ...
-
bioRxiv - Immunology 2023Quote: Isolated CD8+ T cells from OT-I mice were maintained in the naive status after CRISPR-Cas9 gene targeting by resting them overnight in complete medium added with IL-7 (Peprotech) at 10 ng/ml ...
-
bioRxiv - Immunology 2023Quote: ... 4-6 x 106 P14 CD8 T cells were plated in 6 well plates and pre-cultured in IL-7 (10ng/ml, PeproTech) at 37°C ...
-
bioRxiv - Physiology 2024Quote: ... the digested cells were first incubated in RPMI 1640 with 10% FBS and 10 ng/ml recombinant murine IL-7 (PeproTech), and stimulated with 50 ng/ml phorbol 12-myristate 13-acetate ...
-
bioRxiv - Systems Biology 2020Quote: ... at concentrations 0-10 ng/mL or recombinant human interleukin 10 (IL-10, PeproTech, #200-10) at concentrations 0-1000 ng/mL for 30 min ...
-
bioRxiv - Immunology 2022Quote: ... The monocytes were cultured in cRPMI supplemented with recombinant human IL-4 and GM-CSF (Peprotech) to induce DC differentiation ...
-
bioRxiv - Bioengineering 2019Quote: ... IL-15 and IL-2 (Peprotech).
-
bioRxiv - Cancer Biology 2020Quote: ... IL-3 and IL-6 (PeproTech 300-07 ...
-
bioRxiv - Genomics 2021Quote: ... IL-3 and IL-6 (PeproTech). After 48 hours culture ...
-
bioRxiv - Immunology 2021Quote: Both Lin-CD25+CD44- DN3 cells and Lin-CD25-CD44- DN4 cells were sorted from the thymi of LckCre+Zfp335+/+ and LckCre+Zfp335fl/fl mice and co-cultured with OP9-DL1 feeder cells in α-MEM medium in the presence of IL-7 (1 ng/ml, PeproTech) and Flt3-L (5 ng/ml ...
-
bioRxiv - Immunology 2024Quote: ... A single round of co-sedimentation with retroviral stock was performed in medium containing 5% WEHI-3B-conditioned medium and 10 ng/mL IL-7 (Peprotech, USA). After transduction ...
-
bioRxiv - Immunology 2019Quote: ... 100 IU/mL recombinant human (rh) IL-2 (Proleukin, Novartis) and 10 ng/mL rhIL-15 (Peprotech), and were cultured in a humidified incubator at 37 °C + 5% CO2 ...
-
bioRxiv - Biochemistry 2020Quote: scFv-ribosome-mRNA complexes were subjected to selection in solution using recombinant biotinylated human IL-13 (Peprotech) to allow capture using streptavidin-coated paramagnetic beads (Dynabeads M-280) ...
-
bioRxiv - Immunology 2022Quote: ... 14 d IL-2/IL-15 activated human NK cells were plated with K562 leukemia cells at an effector:target (E:T) ratio of 2.5:1 in addition to human IL-2 (100 IU/mL, Peprotech), human IL-15 (20 ng/mL ...
-
bioRxiv - Immunology 2019Quote: ... and 100 μm non-essential amino acids) with 100 U/ml of recombinant human IL-2 (PeproTech), which was replaced every 2 days keeping the cells at a concentration of 1.5 × 106 cells/ml ...
-
bioRxiv - Cancer Biology 2022Quote: ... recombinant human IL-2 (rhIL-2, final concentration of 20 ng/mL; Peprotech, Cat# 200-02-1mg), and 2-mercaptoethanol to a final concentration of 0.05mM (Gibco ...
-
bioRxiv - Immunology 2021Quote: ... cells were incubated for the indicated time in the absence or presence of IL-7 (10 ng/ml, Peprotech, Cat#: 200-07) or stem cell factor (20 ng/ml ...
-
bioRxiv - Immunology 2022Quote: ... Cells were transdifferentiation for 7 days at 60,000 cells/cm2 in medium containing 50 ng/ml IL-3 (200-03 B, Peprotech, New Jersey, USA), 50 ng/ml M-CSF (300-25 B ...