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Citations for Peprotech :
251 - 300 of 2216 citations for IL 17A Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... and then cultured in CTS T Cell Expansion medium (Thermo) containing 10% FBS and 100 IU/ml human IL-2 (PeproTech). The CellTiter 96 MTS assay (Promega ...
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bioRxiv - Immunology 2021Quote: ... 1-5 × 105 PBMCs were stimulated with 1 μg/ml of S864-882 for 10 days and recombinant human IL-2 (1 ng/ml, Peprotech) was added at day 4 and day 7 ...
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bioRxiv - Cancer Biology 2019Quote: Transformation assay was performed by removing IL-3 through centrifugation and adding 50 ng/mL human HGF (Peprotech 100-39H). For proliferation assays cells were seeded in 96-well plates at 5,000 cells/well and the following day were exposed to crizotinib (Selleck Chemicals ...
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bioRxiv - Microbiology 2019Quote: ... Purified CD14+ monocytes were cultured in complete RPMI supplemented with 100ng/mL each of recombinant human IL-4 and GM-CSF (PeproTech) at a cell density of 2e6 cells/mL ...
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bioRxiv - Molecular Biology 2021Quote: ... 2-day cultured activated cells were washed and then cultured for 2 days in culture media supplemented with 20ng/mL recombinant human IL-2 (PeproTech) in the presence or absence of 10μM GC7 (Merck Chemicals) ...
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bioRxiv - Immunology 2021Quote: ... Forty-eight hours later cells were lifted off the plates and cultures were supplemented with 100 U/ml recombinant human IL-2 (Peprotech). CFSE labeling for certain experiments was performed prior to seeding on tissue culture plates.
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bioRxiv - Immunology 2021Quote: ... 1×106 cells/chamber were subjected to chemotaxis in RPMI media with or without the chemoattractant (human IL-27 (500 ng/ml from PeproTech) or N-formyl-methionyl-leucyl-phenylalanine (fMLP ...
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bioRxiv - Microbiology 2021Quote: ... the viral suspension was removed and replaced via 100 μL of NDM or NDM supplemented with 100 ng/mL of recombinant human IL-1β (200-01B, Peprotech), IL-6 (200-06 ...
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bioRxiv - Cancer Biology 2022Quote: ... Enriched cells were taken into culture in T cell medium (TCM, see Methods – primary human material and cell lines) supplemented with 5 ng/mL IL-7 (Peprotech), 5 ng/mL IL-15 (Peprotech) ...
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bioRxiv - Microbiology 2023Quote: ... 1 x 106 BLaER1 cells per well of a 6-well tissue culture plate were treated with 10 ng/ml human recombinant M-CSF and IL-3 (PeproTech) and 100 nM β-estradiol (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... were cultured in six-well plates (2 ml per well) in complete RPMI 1640 medium + 10% FBS + 10 ng/ml human IL-4 (PeproTech) + 100 ng/ml human GM-CSF (PeproTech) ...
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bioRxiv - Immunology 2023Quote: ... cells were washed and then expanded and differentiated in complete IMDM (as above) containing 20 ng/mL recombinant human IL-2 (Peprotech) for a further 4 days ...
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bioRxiv - Molecular Biology 2024Quote: SP2/O-Ag14 myeloma and primary clones of hybridomas were cultured in GIT medium (catalog no. 63725715, Wako) supplemented with recombinant human interleukin-6 (IL-6) (catalog no. 20006, PeproTech). Cells were cultured in a humidified atmosphere containing 5% CO2 at 37°C and sub-cultured every day ...
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bioRxiv - Microbiology 2023Quote: ... Monocyte-derived DCs were differentiated by stimulating THP-1 cells with human GMCSF (1500 IU/ml) and IL-4 (1500 IU/ml) (Peprotech) for 5 days with half the medium replaced at day 3 ...
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bioRxiv - Cancer Biology 2024Quote: ... according to manufacturer’s specifications in the presence of 100 IU/mL recombinant human (rh) IL-2 and 10 ng/mL rhIL-7 (Peprotech). Spinoculation with neat 293 Galv9 retroviral supernatant was performed at 48 ...
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bioRxiv - Immunology 2024Quote: ... splenocytes were restimulated for 5 hours at 37°C with 1 μg/ml of MHC class I-restricted LCMV peptide gp33-41 in the presence of 2 ng/ml recombinant human IL-2 (Peprotech), 10% fetal bovine serum (premium FBS ...
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bioRxiv - Genetics 2020Quote: ... IL-6 (Peprotech) 10ng/ml ...
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bioRxiv - Immunology 2019Quote: ... IL-21 (PeproTech). Goat anti-human F(ab’)2 fragments (anti-IgM & IgG ...
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bioRxiv - Immunology 2020Quote: ... IL-1β (Peprotech) was resuspended in sterile D-PBS/0.2% BSA ...
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bioRxiv - Immunology 2023Quote: ... IL-21 (Peprotech) at 10 ng/ml and IL-10 (Peprotech ...
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bioRxiv - Immunology 2023Quote: ... IL-1β (PeproTech), PGE1 (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2023Quote: ... IL-1α (Peprotech), IL-1β (Peprotech) ...
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bioRxiv - Cancer Biology 2023Quote: ... IL-15 (Peprotech), IL-2 (Chiron) ...
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bioRxiv - Cancer Biology 2023Quote: ... IL-1β (Peprotech), IL-18 (MBL ...
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bioRxiv - Immunology 2023Quote: ... IL-1β (Peprotech) was used at 20ng/mL.
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bioRxiv - Immunology 2021Quote: ... with IL-4 (10ng/ml) and IL-21 (10ng/ml) (PeproTech: 210-21 ...
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bioRxiv - Immunology 2019Quote: ... IL-18 or IL-33 or (Peprotech®, Rocky Hill, NJ) were added to cultures for 6 to 16 hours ...
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bioRxiv - Pathology 2022Quote: ... recombinant murine IL-6 or IL-22 were purchased from Peprotech. Cells were stimulated at 37ºC/5% CO2 in 12-well plates for the indicated times with either IL-6 (100 ng/ml ...
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bioRxiv - Immunology 2023Quote: ... IL-2 (10ng/mL) and IL-7 were purchased from Peprotech. PMA (50ng/mL) ...
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bioRxiv - Immunology 2024Quote: ... or 10ng/mL IL-7 (BioTechne) + 100U/mL IL-2 (Peprotech) (IL-7/IL-2) ...
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bioRxiv - Biophysics 2021Quote: ... after 24 h recombinant human IL-4 (20 ng/ml) and hSCF (100 ng/ml) (both Peprotech, Rocky Hill, NJ, USA) were added ...
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bioRxiv - Immunology 2022Quote: ... X-Vivo 15 media was supplemented with 100 ng/ml human granulocyte-macrophage colony-stimulating factor (GM-CSF) and 20 ng/ml human interleukin 4 (IL-4) (both cytokines obtained from Peprotech, Germany), and cells were cultured with 5% CO2 ...
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bioRxiv - Cancer Biology 2019Quote: ... Cultures were maintained in complete RPMI with 200-300 U/ml human recombinant IL-2 (PeproTech, Rocky Hill, NJ #200-02) and media was refreshed every 3 days ...
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bioRxiv - Cell Biology 2021Quote: ... Macrophages were then skewed towards the M2 phenotype using recombinant human IL-4 (20 ng/ml) (Peprotech, Cat#AF-200-04), IL-13 (20 ng/ml ...
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bioRxiv - Immunology 2019Quote: IL-2 complex (IL-2C) was prepared as described(79) by incubating 1 μg recombinant mouse IL-2 (PeproTech) with 5 μg purified anti-mouse IL-2 antibody (JES6-1 ...
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bioRxiv - Cell Biology 2020Quote: ... 5 ng/ml IL-3, 10 ng/ml IL-11, 0.3 IU/ml Epo, 20 ng/ml IL-7, all from PeproTech). During the clonogenic expansion ...
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bioRxiv - Cancer Biology 2022Quote: ... IL-7 (10 U/ml) and IL-15 (23.8 U/ml) (Peprotech) were freshly added to culture media ...
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bioRxiv - Immunology 2023Quote: ... IL-9 (219-19) and IL-4 were procured from PeproTech (USA). Anti-CD3 and Anti-CD28 were purchased from BD (USA) ...
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bioRxiv - Cancer Biology 2019Quote: ... Samples were examined by ELISA capturing with Fc-iNKG2D and detecting with biotinylated rabbit-anti-human IL-2 polyclonal antibody (Peprotech #500-P22BT) followed by incubation with streptavidin-HRP ...
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bioRxiv - Microbiology 2020Quote: ... Frozen activated PBMC cells were thawed and resuspended in complete PBMC media containing RPMI supplemented with 21 ng/mL human IL-2 (Peprotech; #200-02), 10% fetal bovine serum and 1% penicillin-streptomycin ...
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bioRxiv - Microbiology 2020Quote: ... Pellets were resuspended in activation medium containing RPMI supplemented with 21 ng/mL human IL-2 (Peprotech, Rocky Hill, NJ; #200-02), 1.5 μg/mL PHA (Remel ...
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bioRxiv - Immunology 2020Quote: ... cells were washed and cultured for a further 4 days in media containing 20 ng/mL recombinant human IL-2 (Peprotech, London, UK). For analysis of effector cytokine production ...
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bioRxiv - Immunology 2020Quote: ... Alternately M2 MΦs were activated as follows: M2a MΦs from stimulation with 20 ng/mL recombinant human IL -4 (PeproTech; Rock Hill, NJ, USA) and 25 ng/mL recombinant human IL-13 (PeproTech ...
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bioRxiv - Molecular Biology 2022Quote: ... Cytokine stimulation of human articular chondrocytes was performed on cells at confluence by adding 10ng/ml interleukin-1β (IL-1β, Peprotech, London UK) to standard media and stimulating cells for up to 6 hours.
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bioRxiv - Immunology 2023Quote: ... 10 million cells were resuspended in 10 ml medium and stimulated with 25ng/ml recombinant human interleukin-2 (IL-2) (Peprotech #AF200-02). Vγ9+Vδ2+ T cells were kept overnight at 37°C and 5% CO2.
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bioRxiv - Biochemistry 2024Quote: ... Medium was replaced 24 h later with DMEM-10% FCS supplemented or not with 50 ng/ml human IL-1β (PeproTech, 200-01B). Luciferase activity was measured 5 h later using Bright-Glo Luciferase Assay System (Promega ...
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bioRxiv - Immunology 2021Quote: ... and IL-15 (Peprotech), and CEFX Ultra SuperStim peptide pool (JPT) ...
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bioRxiv - Cell Biology 2021Quote: ... IL-12 from Peprotech and IL-18 from R&D systems ...
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bioRxiv - Neuroscience 2021Quote: ... murine IL-2 (Peprotech) (days 1-3 ...
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bioRxiv - Immunology 2020Quote: ... Murine IL-4 (Peprotech), anti-CD40 (hm40-3 clone ...