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Citations for Peprotech :
151 - 200 of 1790 citations for Human Protein Patched Homolog 2 PTCH2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... 2 ng ml−1 recombinant human TGFβ1 (112 amino acid, HEK293-derived, Peprotech, 100-21). Cells were routinely maintained in E8 medium on 1:800 diluted growth factor reduced Matrigel (see below) ...
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bioRxiv - Immunology 2019Quote: ... cells were stimulated with recombinant human IL-2 (100 U/ml) (Peprotech, Rocky Hill, NJ) for 45 min at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... and 20 ng/ml recombinant human Fibroblast Growth Factor-basic (FGF-2, 100-18B, Peprotech) onto poly-Lysine D coated cell culture plates (Corning) ...
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bioRxiv - Genomics 2021Quote: ... in a 3:1 beads:cells ratio and 100 U/mL recombinant human IIL-2 (Peprotech). Non-tissue culture treated 12-well plates were prepared by incubating 1 mL PBS + 25 μg/mL Retronectin (Takara ...
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bioRxiv - Genomics 2021Quote: ... in a 3:1 beads:cells ratio and 100 U/mL recombinant human IL-2 (Peprotech).
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bioRxiv - Immunology 2022Quote: ... cells were cultured in complete RPMI containing recombinant human IL-2 (30 U/ml, Peprotech) and stimulated with anti-CD3/anti-CD28 Dynabeads (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... We further supplemented the media with 5 ng/mL recombinant human (rh) IL-2 (Peprotech) and 10 ng/mL rhIL-7 ...
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bioRxiv - Systems Biology 2023Quote: ... and subsequently cultured in complete culture media supplemented with 5 nM human IL-2 (Peprotech) for up to 10 days ...
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bioRxiv - Bioengineering 2022Quote: ... were coated with 2 μg ml-1 of recombinant human PD-L1ECDFc (Peprotech 310-35), ZNRF3ECDFc (R&D systems 7994-RF-025 ...
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bioRxiv - Cell Biology 2023Quote: ... and 2 ng/mL Recombinant Human Transforming Growth Factor-β1 (TGF-β1, PeproTech, 100-21). Cells were passaged when reaching 70% confluency until day 18 ...
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bioRxiv - Cell Biology 2024Quote: ... BMP-2/4) (ERB medium)3 or adding 10 ng/ml human IL-22 (Peprotech) in WENRA4 (Wnt/ R-spondin1 ...
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bioRxiv - Cancer Biology 2024Quote: ... EGM-2 medium supplemented with VEGF (50 ng/ ml, Recombinant Human VEGF, Peprotech 100-20A) was then added in all the inlets and an interstitial flow was created by having different volumes of medium in the inlets (90ul in the inlets of the HUVEC channel and 110ul in the inlets of the opposite channel) ...
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bioRxiv - Microbiology 2019Quote: ... using samples from the basal compartment diluted ten times with PBS (ABTS ELISA Buffer Kit, PeproTech, London, UK), and measured as indicated above.
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bioRxiv - Cancer Biology 2023Quote: ... or c) supplemented with recombinant human IGFBP5 protein (500 ng/ml; Peprotech, Rocky Hill, NJ, USA). Cell lysates were obtained using RIPA buffer with the Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher ...
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bioRxiv - Developmental Biology 2021Quote: ... Bone morphogenetic protein 2 (BMP2, 100-200 ng/mL; Peprotech #120-02), Bone morphogenetic protein 4 (BMP4 ...
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bioRxiv - Genomics 2019Quote: ... recombinant human IFN-γ (specific activity 2×107 U/mg; AF-300-02, Peprotech, London, UK) at 1000 U/ml as described3 ...
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bioRxiv - Neuroscience 2023Quote: ... The final medium was comprised of basal media containing human TGF-β2 (2 ng/mL, Peprotech), murine IL-34 (100 ng/mL ...
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bioRxiv - Cell Biology 2023Quote: ... Recombinant HCC-1 protein (20ng/ml, PP-300-38B-2, Peprotech, United States) was added to cell culture medium ...
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bioRxiv - Microbiology 2020Quote: ... TNFα in the supernatants was determined using a Murine TNFα Standard TMB ELISA Development Kit (PeproTech, catalog number 900-T54) and a Human IL-8 Standard TMB ELISA Development Kit (PeproTech ...
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bioRxiv - Microbiology 2021Quote: ... IL-10 (#900-K53) and IP-10 (CXCL10) (#250-16) in the supernatants were quantified using ABTS ELISA Development Kit (PeproTech) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... 100 IU/mL recombinant human (rh) IL-2 (Proleukin, Novartis) and 10 ng/mL rhIL-15 (Peprotech), and were cultured in a humidified incubator at 37 °C + 5% CO2 ...
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bioRxiv - Developmental Biology 2020Quote: ... These transfected cells were mixed with 2 ml culture medium (20 ng/ml human basic FGF (Peprotech), 1XB27 RA-(Gibco) ...
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bioRxiv - Immunology 2022Quote: ... 14 d IL-2/IL-15 activated human NK cells were plated with K562 leukemia cells at an effector:target (E:T) ratio of 2.5:1 in addition to human IL-2 (100 IU/mL, Peprotech), human IL-15 (20 ng/mL ...
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bioRxiv - Immunology 2019Quote: ... and 100 μm non-essential amino acids) with 100 U/ml of recombinant human IL-2 (PeproTech), which was replaced every 2 days keeping the cells at a concentration of 1.5 × 106 cells/ml ...
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bioRxiv - Immunology 2021Quote: ... and IL-4 and IL-10 (Th2 immune markers)cytokines secreted to the supernatant were determined using specific ELISA kits according to the manufacturer’s instructions (Peprotech, Rehovot, Israel).
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bioRxiv - Immunology 2021Quote: ... in the presence of human IL-2 (30 U/ml) and murine IL-7 (10 ng/ml; PeproTech) in RPMI 1640 media supplemented with 10% heat-inactivated FBS ...
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bioRxiv - Immunology 2021Quote: ... diluted in complete RPMI (cRPMI; RPMI supplemented with 10% FBS plus recombinant human IL-2 (20IU/ml; Peprotech), as previously described.65,66 Activated cells were incubated for six days with a media change on day three.
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bioRxiv - Immunology 2021Quote: ... whole bone marrow was plated at 1M cells/mL in cRPMI + 400U/mL Proleukin human IL-2 (Peprotech). Fresh IL-2 was added every 3rd day ...
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bioRxiv - Immunology 2023Quote: ... and expanded in RPMI 1640 containing 10% FBS and 100 IU/mL of recombinant human IL-2 (Peprotech). Human blood samples were enriched for T cells using negative bead selection (StemCell ...
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bioRxiv - Cell Biology 2021Quote: ... human FGF-basic and human EGF (animal free, Peprotech) at 20 ng/mL ...
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bioRxiv - Biochemistry 2023Quote: ... The expression of IL-6 in culture supernatants was detected using the Murine IL-6 Mini ABTS ELISA Development Kit (PeproTech, 900-M50) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2023Quote: ... Supernatants were harvested and used in triplicate to measure the concentration of VEGF-A by ELISA using the kit from PeproTech (Cranbury, NJ). In another experiment ...
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bioRxiv - Biochemistry 2020Quote: ... according to the manufacturer’s instructions and cultured in RPMI supplemented with 10% FBS and 30 U/ml recombinant human IL-2 (PeproTech) at 37°C in 5% CO2 ...
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bioRxiv - Immunology 2021Quote: ... Switzerland) for 11 days in the human plasma serum-containing medium with 3000 IU/ml of IL-2 (PeproTech). The cell cultures were supplemented with fresh media and IL-2 every 2–4 days ...
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bioRxiv - Immunology 2021Quote: ... at a bead to cell ratio of 3:1 and 1,000 U/mL of recombinant human IL-2 (Peprotech). Fresh IL-2 containing media was added every 48 h and Human T-activator CD3/CD28 Dynabeads were added again at day 7 and removed at day 15 of expansion ...
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bioRxiv - Genetics 2022Quote: ... clone 145-2C11, WEHI antibody facility), anti-CD28 (2μg/ml, clone 3751, WEHI antibody facility) and human IL-2 (Peprotech). CD8 T cell cultures contained 25μg/ml anti-mouse IL2 (clone S4B6 ...
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bioRxiv - Immunology 2023Quote: ... in the presence of 2mg/mlaCD28 (clone 37.51, WEHI antibody facility) and 100U/ml recombinant human IL-2 (Peprotech), in 96 well flat-bottom plates at 104 cells per well ...
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bioRxiv - Immunology 2024Quote: CD8 T cells from WT p14 or Irf5-/- p14 mice were isolated as described above and cultured with or without 1µg/ml gp33 peptide in complete RPMI medium supplemented with 2ng/ml recombinant human IL-2 (Peprotech). Cells were then collected at 6 or 24 hours after incubation and washed with PBS ...
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bioRxiv - Immunology 2023Quote: ... at a density of 7.5×105 cells/ml in 2 ml complete media supplemented with 20 ng/ml recombinant human GM-CSF (Peprotech). The plates were incubated at 37°C in 5% CO2 ...
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bioRxiv - Immunology 2022Quote: ... mice were repeatedly immunized every 14 days (5 times) subcutaneously (s.c.) into the flank with 10μg of human TNFR2 protein (Peprotech, 310-12) and 20μg MPLA (Sigma ...
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bioRxiv - Immunology 2020Quote: Human PBMCs were stimulated with recombinant human IL-12 (Peprotech) and IL-18 (R&D Systems ...
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bioRxiv - Cancer Biology 2023Quote: ... human EGF (Peprotech), and FGF (Peprotech) ...
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bioRxiv - Microbiology 2020Quote: ... the PBMCs were cultured with the synthetic peptide mixture of SARS-CoV-2 at a concentration of 1μg/ml or DMSO in RPMI 1640 medium containing 10% FCS and 20 U/ml recombinant human IL-2 (Peprotech) for 7 days ...
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bioRxiv - Cancer Biology 2021Quote: ... 106 anti-CD19 CD8 CAR T cells were expanded in presence of 7 x 106 irradiated (8000 cGy) TM-LCL feeder cells in RPMI1640 containing 50 IU/mL human recombinant IL-2 (Peprotech), 10 ng/ml recombinant human lL-15 (Peprotech) ...
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bioRxiv - Molecular Biology 2022Quote: ... The isolated cells were cultured in RPMI medium supplemented with 20% (v/v) FBS and 100 ng/mL human recombinant IL-2 (Peprotech). Two days prior to transduction ...
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bioRxiv - Immunology 2022Quote: ... cells were maintained in 24-well G-Rex plates (Wilson Wolf) in NK MACS media (Miltenyi Biotech) supplemented with human IL-2 (100 IU/mL, Peprotech) and human IL-15 (20 ng/mL ...
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bioRxiv - Immunology 2022Quote: ... After a one hour resting period cells were transferred to 24-well plates and incubated for three days in medium containing 10U/mL recombinant human IL-2 (Peprotech) to allow establishment of the knockout ...
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bioRxiv - Cancer Biology 2021Quote: ... and then cultured in CTS T Cell Expansion medium (Thermo) containing 10% FBS and 100 IU/ml human IL-2 (PeproTech). The CellTiter 96 MTS assay (Promega ...
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bioRxiv - Immunology 2021Quote: ... 1-5 × 105 PBMCs were stimulated with 1 μg/ml of S864-882 for 10 days and recombinant human IL-2 (1 ng/ml, Peprotech) was added at day 4 and day 7 ...
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bioRxiv - Cell Biology 2020Quote: ... After 2 days of culture with 20 ng/ml M-CSF and 20 ng/ml recombinant human RANKL (PeproTech, UK), the wells were rinsed twice with PBS and incubated with 10% bleach solution for 30 min at room temperature ...