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Citations for Peprotech :
151 - 200 of 1819 citations for Human Butyrophilin Like Protein 2 BTNL2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... All media was supplemented with 100 U mL-1 human IL-2 (Peprotech). T cells were activated with a 3:1 ratio of CD3/CD28 dynabeads to T cells and split or expanded every 2 days to maintain T cells at a concentration of 1-2 x 106 per mL unless otherwise indicated.
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bioRxiv - Immunology 2023Quote: ... and recombinant human IL-2 (final concentration 50 U/mL, Peprotech, #200-02) were added at the start of the co-culture ...
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bioRxiv - Cancer Biology 2023Quote: ... 50 μM β-mercapoethanol and 50 U/ml of human IL-2 (Peprotech) for 48 hours.
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bioRxiv - Immunology 2024Quote: ... at a 1:1 ratio and recombinant human IL-2 (Peprotech, ThermoFisher Scientific), and expanded in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Bioengineering 2021Quote: ... 100 ng/mL human recombinant Bone Morphogenic Protein 4 (BMP4, Peprotech, AF-120-05ET), 100 ng/mL animal-free recombinant human Stem Cell Factor (SCF ...
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bioRxiv - Developmental Biology 2023Quote: ... 5 nM recombinant human bone morphogenetic protein 4 (BMP4; Peprotech, # AF-120-05ET, USA) and 2 µM Smoothened agonist (SAG ...
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bioRxiv - Immunology 2022Quote: ... we used murine ELISA kits according to the manufacturers’ recommendations for IL-1β (PeproTech, 900-K47), IL-6 (Biotest ...
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bioRxiv - Immunology 2023Quote: ... Supernatants were harvested and used in triplicate to quantitate IL-1β using ELISA kit from PeproTech.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and prostaglandin E2 (PGE2) enzyme-linked immunosorbent assay (ELISA) kit were purchased from Peprotech (NSW, Australia). Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Bioengineering 2020Quote: ... Primary human T cells were cultured in complete RPMI 1640 supplemented with 100 U/mL recombinant human IL-2 (PeproTech, 200-02). Cells were cultured at 37°C in a humidified 5% CO2 incubator.
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bioRxiv - Bioengineering 2023Quote: ... Primary human T cells were cultured in complete RPMI 1640 supplemented with 100□U/ml recombinant human IL-2 (PeproTech, 200-02). All mammalian cells were cultured at 37□°C in a humidified 5% CO2 incubator.
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bioRxiv - Microbiology 2020Quote: ... were cocultured with 2×105 peptide-pulsed autologous DCs in a 24-well plate in the presence of 10 ng/mL recombinant human interleukin-2 (IL-2; Peprotech). The next day ...
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bioRxiv - Cancer Biology 2021Quote: ... emerging hematopoietic cells and EBs were cultivated in SFM ((Liu et al. 2015) supplemented with recombinant human 10 ng fiblroblast growth factor type 2 (rhFGF-2, Peprotech), recombinant human 50 ng/mL rhSCF and 30 ng/mL rhIL-3 ...
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bioRxiv - Molecular Biology 2021Quote: ... 2-day cultured activated cells were washed and then cultured for 2 days in culture media supplemented with 20ng/mL recombinant human IL-2 (PeproTech) in the presence or absence of 10μM GC7 (Merck Chemicals) ...
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bioRxiv - Immunology 2024Quote: ... splenocytes were restimulated for 5 hours at 37°C with 1 μg/ml of MHC class I-restricted LCMV peptide gp33-41 in the presence of 2 ng/ml recombinant human IL-2 (Peprotech), 10% fetal bovine serum (premium FBS ...
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bioRxiv - Bioengineering 2023Quote: ... M2-like macrophages were activated by adding 25ng/mL interleukin 4 (IL-4; PeproTech: 200-04-50UG) and 25ng/mL IL-13 (PeproTech ...
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bioRxiv - Cancer Biology 2023Quote: ... and ligand for fms-like tyrosine kinase receptor-3 (10ng/ul) (all from Peprotech, Cranbury, New Jersey). OP-puro was immediately added to the medium (20uM final concentration ...
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bioRxiv - Bioengineering 2023Quote: ... M1-like macrophages were activated by adding 5ng/mL interferon gamma (IFN-γ; PeproTech: AF-300-02) and 10ng/mL lipopolysaccharide (LPS ...
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bioRxiv - Immunology 2021Quote: ... 100– 200 U/mL recombinant human IL-2 (PeproTech, Rocky Hill, New Jersey, USA), and a final concentration of 12.5% horse serum and 12.5% fetal bovine serum.
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bioRxiv - Genetics 2020Quote: ... Stimulation was performed for 4 days using recombinant human IL-2 (10ng/ml, Peprotech) and Anti-Biotin MACSiBead Particles loaded with CD2 ...
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bioRxiv - Synthetic Biology 2021Quote: ... and 2 ng/mL recombinant human granulocyte macrophage colony-stimulating factor (GM-CSF; PeproTech) unless specified otherwise ...
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bioRxiv - Bioengineering 2021Quote: ... injection and mice were dosed with 1×104 units recombinant human IL-2 (Peprotech) twice daily for 3 days (6 total injections) ...
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bioRxiv - Microbiology 2021Quote: ... Glutamine (2 mM) and supplemented with 100 ng/mL human M-CSF cytokine (Peprotech). Three days post-isolation ...
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bioRxiv - Immunology 2022Quote: ... 1 x glutamax and 50 IU/ml human recombinant IL-2 (Peprotech, Cranbury, NJ). Enriched CD4+ and CD8+ T cells were activated with ImmunoCult NHP CD2/CD3/CD28 T-cell activator (STEMCELL Technologies ...
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bioRxiv - Bioengineering 2024Quote: ... supplemented with 5% FBS (MilliporeSigma) and 50 U/mL of human IL-2 (Peprotech). T cells were activated at 1:1 ratio of cells to dynabeads ...
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bioRxiv - Bioengineering 2024Quote: Analysis of anti-microbial peptide human β-defensin 2 (PeproTech, Cranbury, NJ - 900-K172) was performed in OTMs via ELISA ...
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bioRxiv - Immunology 2019Quote: BAL supernatants were analysed for Ym1 using commercially available ELISA kits (R&D systems and Peprotech, respectively). Lung homogenates were assayed for IL-4 using standard sandwich ELISA protocols (eBioscience) ...
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bioRxiv - Immunology 2020Quote: ... cells were polarized to M1-like macrophages using 1 ug/mL LPS and 100 ng/mL IFNγ (Peprotech) or M2-like macrophages using 100 ng/mL IL-4 (Peprotech ...
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bioRxiv - Immunology 2023Quote: ... 10 million cells were resuspended in 10 ml medium and stimulated with 25ng/ml recombinant human interleukin-2 (IL-2) (Peprotech #AF200-02). Vγ9+Vδ2+ T cells were kept overnight at 37°C and 5% CO2.
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bioRxiv - Cell Biology 2019Quote: ... 2 ng ml−1 recombinant human TGFβ1 (112 amino acid, HEK293-derived, Peprotech, 100-21). Cells were routinely maintained in E8 medium on 1:800 diluted growth factor reduced Matrigel (see below) ...
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bioRxiv - Immunology 2019Quote: ... cells were stimulated with recombinant human IL-2 (100 U/ml) (Peprotech, Rocky Hill, NJ) for 45 min at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... and 20 ng/ml recombinant human Fibroblast Growth Factor-basic (FGF-2, 100-18B, Peprotech) onto poly-Lysine D coated cell culture plates (Corning) ...
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bioRxiv - Genomics 2021Quote: ... in a 3:1 beads:cells ratio and 100 U/mL recombinant human IIL-2 (Peprotech). Non-tissue culture treated 12-well plates were prepared by incubating 1 mL PBS + 25 μg/mL Retronectin (Takara ...
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bioRxiv - Genomics 2021Quote: ... in a 3:1 beads:cells ratio and 100 U/mL recombinant human IL-2 (Peprotech).
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bioRxiv - Immunology 2022Quote: ... cells were cultured in complete RPMI containing recombinant human IL-2 (30 U/ml, Peprotech) and stimulated with anti-CD3/anti-CD28 Dynabeads (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... We further supplemented the media with 5 ng/mL recombinant human (rh) IL-2 (Peprotech) and 10 ng/mL rhIL-7 ...
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bioRxiv - Systems Biology 2023Quote: ... and subsequently cultured in complete culture media supplemented with 5 nM human IL-2 (Peprotech) for up to 10 days ...
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bioRxiv - Bioengineering 2022Quote: ... were coated with 2 μg ml-1 of recombinant human PD-L1ECDFc (Peprotech 310-35), ZNRF3ECDFc (R&D systems 7994-RF-025 ...
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bioRxiv - Cell Biology 2023Quote: ... and 2 ng/mL Recombinant Human Transforming Growth Factor-β1 (TGF-β1, PeproTech, 100-21). Cells were passaged when reaching 70% confluency until day 18 ...
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bioRxiv - Cell Biology 2024Quote: ... BMP-2/4) (ERB medium)3 or adding 10 ng/ml human IL-22 (Peprotech) in WENRA4 (Wnt/ R-spondin1 ...
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bioRxiv - Cancer Biology 2024Quote: ... EGM-2 medium supplemented with VEGF (50 ng/ ml, Recombinant Human VEGF, Peprotech 100-20A) was then added in all the inlets and an interstitial flow was created by having different volumes of medium in the inlets (90ul in the inlets of the HUVEC channel and 110ul in the inlets of the opposite channel) ...
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bioRxiv - Microbiology 2019Quote: ... using samples from the basal compartment diluted ten times with PBS (ABTS ELISA Buffer Kit, PeproTech, London, UK), and measured as indicated above.
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bioRxiv - Cancer Biology 2023Quote: ... or c) supplemented with recombinant human IGFBP5 protein (500 ng/ml; Peprotech, Rocky Hill, NJ, USA). Cell lysates were obtained using RIPA buffer with the Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher ...
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bioRxiv - Developmental Biology 2021Quote: ... Bone morphogenetic protein 2 (BMP2, 100-200 ng/mL; Peprotech #120-02), Bone morphogenetic protein 4 (BMP4 ...
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bioRxiv - Genomics 2019Quote: ... recombinant human IFN-γ (specific activity 2×107 U/mg; AF-300-02, Peprotech, London, UK) at 1000 U/ml as described3 ...
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bioRxiv - Neuroscience 2023Quote: ... The final medium was comprised of basal media containing human TGF-β2 (2 ng/mL, Peprotech), murine IL-34 (100 ng/mL ...
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bioRxiv - Cell Biology 2023Quote: ... Recombinant HCC-1 protein (20ng/ml, PP-300-38B-2, Peprotech, United States) was added to cell culture medium ...
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bioRxiv - Cancer Biology 2020Quote: ... 100 ng/mL fms-like tyrosine kinase 3 ligand (Flt-3L) and 50 ng/mL thrombopoietin (TPO) (all from Peprotech). ES and IPS derived EB were cultured in ultra low attachment 6-well plates (Costar ...
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bioRxiv - Cancer Biology 2022Quote: ... 100 ng/mL fms-like tyrosine kinase 3 ligand (Flt-3L) and 50 ng/mL thrombopoietin (TPO) (all from Peprotech). ES and IPS derived EB were cultured in ultra-low attachment 6-well plates (Costar ...
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bioRxiv - Immunology 2023Quote: ... Cells were cultured with stem cell factor (SCF) and FMS-like tyrosine kinase 3 ligand (FLT3L) (Peprotech, Rocky Hill, USA) for the first 4 days ...