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Citations for Peprotech :
251 - 300 of 302 citations for Granzyme B GZMB Mouse CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: IL-2 complex (IL-2C) was prepared as described(79) by incubating 1 μg recombinant mouse IL-2 (PeproTech) with 5 μg purified anti-mouse IL-2 antibody (JES6-1 ...
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bioRxiv - Immunology 2022Quote: ... the IFNγ that had been captured by the plate-bound XMG1.2 was detected by the biotinylated rabbit anti-mouse IFNγ (PeproTech), streptavidin-HRP (Abcam) ...
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bioRxiv - Immunology 2021Quote: ... (2) treated with recombinant mouse IL-12 (5 ng/ml) and IL-18 (25 ng/ml, both from Peprotech), (3 ...
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bioRxiv - Cancer Biology 2023Quote: ... Three weeks after transplantation mice were treated with 500 ng/mouse/day of recombinant IL1β (Peprotech, Cranbury, New Jersey) intraperitoneally ...
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bioRxiv - Immunology 2022Quote: ... followed by addition of 100,000 sorted CD5- or CD71- DN3 thymocytes per well together with 10 ng/ml recombinant mouse IL-7 (PeproTech).
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bioRxiv - Immunology 2023Quote: ... The following anti-mouse primary antibodies were used: anti-RELM-beta (1:500, rabbit polyclonal antibody, Peprotech, 500-p215), anti-MUC2 (1:200 ...
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bioRxiv - Immunology 2023Quote: ... 6-week-old Foxp3IRES-GFP mice were injected intravenously with 10 μg of recombinant mouse IL2 (PeproTech, 212-12) or PBS vehicle control (in 110 μl volume) ...
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bioRxiv - Cancer Biology 2024Quote: ... Growth factors were also given to media surrounding basement membrane extract (Cultrex, Biotechne): 1% Mouse recombinant Noggin (Peprotech, UK), 1% mouse recombinant EGF (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... T cells were removed from stimulation and cultured in complete medium supplemented with10ng/ml recombinant mouse IL-7 (Peprotech) for 3-5 days as described (69).
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bioRxiv - Immunology 2021Quote: ... for 4 days at 37°C in complete RPMI medium supplemented with 10 ng/mL recombinant mouse IL-5 (PeproTech) and 20 ng/mL IL-2 (402-ML R&D) ...
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bioRxiv - Immunology 2021Quote: ... for 4 days at 37°C in complete RPMI medium supplemented with 10 ng/mL recombinant mouse IL-5 (PeproTech) and 20 ng/mL IL-2 (402-ML R&D) ...
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bioRxiv - Neuroscience 2020Quote: ... The final pellets were dissociated with pre-warmed 10% fetal bovine serum in DMEM supplemented with 20 ng mL-1 mouse M-CSF (PeproTech) and plated in culture dishes ...
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bioRxiv - Neuroscience 2020Quote: ... in the presence of freshly added 20 ng/ml mouse glial cell line-derived neurotrophic factor (GDNF, PeproTech, 450-44) as detailed elsewhere (Sleigh et al. ...
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bioRxiv - Cancer Biology 2020Quote: ... Ba/F3 cells and Ba/F7 cells were maintained in medium supplemented with 10 ng/ml recombinant mouse interleukin 3 (IL3) and interleukin 7 (IL7) (PeproTech), respectively ...
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bioRxiv - Immunology 2022Quote: ... OT-I T cells were maintained inactivated in vitro (‘No TCR’ condition) with recombinant mouse IL-7 (Peprotech, 10ng/ml). For IL-2 replenishment experiments ...
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bioRxiv - Immunology 2020Quote: ... cells were seeded in complete DMEM medium (10% FCS, 1000 U/ml Penicillin, 100 µg/ml Streptomycin) containing 30 ng/mL of mouse M-CSF (Peprotech). After two successive medium replacement (at D3 and D6) ...
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bioRxiv - Immunology 2021Quote: Bone marrow cells were isolated from C57BL/6J mouse tibia and femur and then cultured for 7–9 days in 10% FBS DMEM containing mouse GM-CSF (50 ng/mL, Peprotech).
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The skin environment controls local dendritic cell differentiation and function through innate IL-13bioRxiv - Immunology 2021Quote: ... were harvested and one million cells were resuspended in 1 mL of TCM in 24 wells plates and stimulated with recombinant mouse IL-13 (Peprotech) at a concentration of 100 ng/mL for 30 min at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... cells were treated with recombinant mouse IFNβ (1000 U/ml, Stratech) or IL-10 (250 ng/ml, catalogue 210-10 Peprotech) 3 h prior to collection for immunoblotting.
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bioRxiv - Immunology 2020Quote: ... the obtained bone marrow cells were cultured in RPMI-1640 containing 10% FBS and 10 ng mL−1 mouse macrophage colony-stimulating factor (M-CSF, Peprotech) in a humidified 5% CO2 atmosphere at 37 °C for six days ...
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bioRxiv - Immunology 2022Quote: ... or into bone marrow-derived dendritic cells (BMDC) in cRPMI supplemented with recombinant mouse IL-4 and GM-CSF (Peprotech). Influenza virus strains A/California/04/2009 (H1N1) ...
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bioRxiv - Immunology 2022Quote: ... Bone marrow cells isolated from the bones of adult C57BL/6J mice were differentiated into bone marrow-derived macrophages (BMM) in cDMEM supplemented with recombinant mouse M-CSF (Peprotech) or into bone marrow-derived dendritic cells (BMDC ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were immediately plated on Poly-D-lysine/Laminin coated cover slips with the addition of growth factors (mouse nerve growth factor (50 ng/ml; NGF, PeproTech) and 10 ng/ml glial-derived neurotrophic factor (GDNF ...
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bioRxiv - Immunology 2022Quote: ... splenocytes from control and Ncr1cre Mycfl/fl mice were left unstimulated or stimulated with 50 ng/mL recombinant mouse IL-15 (PeproTech) for 40 min at 37 °C and analyzed for intracellular protein phosphorylation by flow cytometry ...
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bioRxiv - Pathology 2023Quote: ... Bone marrow was collected and cultured in RPMI-1640 complete medium (CM) containing 20ng/ml recombinant mouse GM-CSF (Peprotech). Subsequently ...
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bioRxiv - Immunology 2023Quote: ... fixed cells were permeabilised with the Transcription Factor staining kit (eBioscience) for 20min at room temperature and stained for 45min with rabbit anti-mouse RELMα (Peprotech) and biotinylated goat anti-mouse Ym1 (R&D) ...
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bioRxiv - Cancer Biology 2023Quote: ... These tumoroids were then cultured in BEN selection media (basal medium with mouse recombinant EGF (50 ng/ml) and Noggin (100 ng/ml) (PeproTech) without R-spondin ...
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bioRxiv - Immunology 2024Quote: ... R&D), IL-27 (20 ng/mL, R&D) as well as with 2 μg/mL anti-mouse IL-4 (Peprotech). Alternatively ...
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bioRxiv - Immunology 2021Quote: Flow-cytometry-purified BM-eos (B6J) were conditioned overnight with colon supernatant and treated with recombinant mouse IFN-γ (10ng/ml, 315-05 PeproTech), as indicated ...
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bioRxiv - Immunology 2022Quote: ... Whole mouse splenocytes and human PBMCs were plated at a density of 5×106 cells per well in a Cepallet W-type 24 well microplate (DIC, Japan) and cultured for 4 days with or without mouse/human M-CSF (Peprotech, USA). Surviving macrophages adhered to 24-well plates were detached by lowering the temperature on ice ...
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bioRxiv - Immunology 2023Quote: ... single-cell suspensions of spleens from 10-week-old Foxp3Thy1.1 mice were stimulated for 15 minutes at 37°C with the indicated concentration of recombinant mouse IL2 (PeproTech, 212-12) in serum-free RPMI 1640 ...
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bioRxiv - Cancer Biology 2023Quote: ... with 100 μl of processed CM in the presence or absence of anti-LIF antibody (4 μg/ml) or recombinant mouse LIF (0.1 μg/ml; Peprotech, 250-02). Spheroids were quantified after 8 d culture.
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bioRxiv - Cancer Biology 2024Quote: ... 5 x 105 splenocytes from single cell suspensions were incubated with increasing concentrations of recombinant mouse IFNγ (0, 0.1, 1, 10, 100 ng/mL) for 48 hours (Peprotech, 315-05). Cells were then harvested and incubated with a panel of antibodies containing anti-mouse CD19 (Biolegend,115504) ...
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bioRxiv - Immunology 2024Quote: ... together with 10 ng/mL recombinant mouse IL-4 or 3 μg/mL LPS with 10 ng/mL recombinant murine IFN-ψ (Peprotech) and 20 ng/mL recombinant human BAFF or 5 μg/mL anti-CD40 (Bio X Cell ...
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bioRxiv - Cancer Biology 2019Quote: ... and recombinant mouse granulocyte macrophage-colony stimulating factor (GM-CSF) at a concentration of 20 ng/mL (murine rGM-CSF, Peprotech 315-03). MPM was replaced every three days until phase-bright microglia became visible in the adherent culture and floater cells became visible in the media ...
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bioRxiv - Cancer Biology 2021Quote: Bone marrow cells were obtained from both femur and tibia of the mouse and differentiated for five days in DMEM supplemented with 10ng of CSF1 (PeproTech, NJ, USA) for five days to generate BMDMs ...
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bioRxiv - Cell Biology 2021Quote: ... were flushed out and differentiated by culturing in DMEM-10% FCS supplemented with mouse colony stimulating factor (M-CSF, 25ng/ml, Peprotech, 315-02) for 5-6 days ...
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bioRxiv - Immunology 2019Quote: ... Samples were adjusted to 7.5×105 cells/mL with cold cRPMI_M supplemented with 25 ng/mL of recombinant mouse colony-stimulating factor (m-csf, Peprotech, Rocky Hill, NJ) and cultured at 37°C ...
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bioRxiv - Developmental Biology 2021Quote: ... Differences were that for mouse cells the medium for aNSCs was additionally supplemented with 20 ng/ml recombinant murine Epidermal Growth Factor (PeproTech 315-09) and qNSC induction was performed with 50 ng/ml BMP4 (R&D Systems 5020-BP-010) ...
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bioRxiv - Immunology 2022Quote: ... same number of magnetically sorted naive T cells were co-cultured with equal numbers of BMDCs supplemented with 20 ng/ml of recombinant mouse IL-2 (PEPROTECH: 212-12) for 24-72 hours ...
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bioRxiv - Microbiology 2023Quote: ... Plates were incubated for 15 min at 37°C to allow the Matrigel to polymerize and then overlaid with 300 µl of growth medium (basal medium supplemented with 12.5 ng/ml mouse recombinant epithelial growth factor (PeproTech, Rocky Hill, NJ), 25 ng/ml mouse recombinant Noggin (PeproTech) ...
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bioRxiv - Neuroscience 2023Quote: ... Each mouse in the GDNF group was injected sub-cutaneously with 50 µl of 2mg/ml of GDNF (Peprotech Catalogue #: 450-44) every other day ...
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bioRxiv - Neuroscience 2023Quote: ... Each mouse in the HGF group was injected sub-cutaneously with 100 µl of 2mg/ml of HGF (Peprotech Catalogue #:315-23) every other day ...
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bioRxiv - Neuroscience 2023Quote: ... cells were resuspended in 320 μl of fresh DRG media (F12, 10% hi-FBS, 1% Pen/Strep, 20 ng/ml mouse GDNF (Peprotech #450-44), 50 ng/ml mouse NGF (Peprotech #450-34) ...
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bioRxiv - Cell Biology 2024Quote: ... Evans blue was allowed to circulate for 10 min before intradermal injection in the back skin of recombinant mouse VEGFA165 (Peprotech, 450-32) (80 ng in 25 μl ...
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bioRxiv - Molecular Biology 2019Quote: ... 10 weeks old) were subcutaneously injected into the groin regions with 400 μL Matrigel containing recombinant mouse basic FGF (PeproTech, 250 ng/mL) and heparin (Sigma ...
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bioRxiv - Cancer Biology 2021Quote: ... The cells were incubated in RPMI1640 for THP-1 culture supplemented with human M-CSF at 20 ng/ml (cross-react with mouse, Peprotech, Cranbury, NJ, USA). After 2 days of culture ...
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bioRxiv - Developmental Biology 2020Quote: ... LepRb-Cre::tdTom and GCG-Cre::SynTom mice received intraperitoneal (i.p.) injections of recombinant mouse leptin (10mg/kg body weight; Peprotech Inc., Rocky Hill, NJ), or vehicle (0.9% sterile saline) ...
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bioRxiv - Immunology 2021Quote: ... were plated with the irradiated 40LB cells in R10 media with 1 ng/mL recombinant mouse interleukin-4 (rmIL-4; Peprotech, East Windsor, NJ). On day 4 ...
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bioRxiv - Immunology 2021Quote: ... cells isolated from the femur and tibia were cultured at 1 × 106 cells/ml in 10 ml of complete DMEM (cDMEM) supplemented with 4% recombinant mouse GM-CSF (Peprotech, Rocky Hill, NJ, USA) at 37°C in 7.5% CO2 ...