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Citations for Peprotech :
551 - 600 of 615 citations for GM CSF Rat CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... the non-adherent cells were transferred to a new non-tissue culture treated petri dish and incubated with BMDM media supplemented with 20 ng/mL M-CSF (PeproTech). On day 3 after plating ...
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bioRxiv - Immunology 2020Quote: ... These cells were cultured for 7 d in DMEM cell culture medium containing 40 ng/ml macrophage colony-stimulating factor (M-CSF) (PeproTech). For the generation of bone marrow-derived dendritic cells ...
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bioRxiv - Molecular Biology 2021Quote: ... with subsequent maintenance of adherent cells in RPMI 1640 medium supplemented with 10% human serum and M-CSF (10 ng/ml, Peprotech) for 3 days and then differentiated for a further 4 days in RPMI 1640 medium supplemented with 10% fetal calf sera without M-CSF.
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bioRxiv - Immunology 2020Quote: ... the obtained bone marrow cells were cultured in RPMI-1640 containing 10% FBS and 10 ng mL−1 mouse macrophage colony-stimulating factor (M-CSF, Peprotech) in a humidified 5% CO2 atmosphere at 37 °C for six days ...
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bioRxiv - Cell Biology 2021Quote: ... bone marrows were cultured and in vitro differentiated in bone marrow differentiation medium containing 40 ng/ml recombinant murine M-CSF (PeproTech) as described before (Sun et al. ...
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bioRxiv - Immunology 2022Quote: ... Bone marrow cells isolated from the bones of adult C57BL/6J mice were differentiated into bone marrow-derived macrophages (BMM) in cDMEM supplemented with recombinant mouse M-CSF (Peprotech) or into bone marrow-derived dendritic cells (BMDC ...
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bioRxiv - Immunology 2022Quote: ... bone marrow cells harvested from femurs and tibias of 6–10-week-old control or EROS-/- mice were grown in complete RPMI medium supplemented with 100 ng/mL of M-CSF (Peprotech) for 3 days ...
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bioRxiv - Cell Biology 2022Quote: ... The monolayer was washed 3 times with HBSS to remove non-adherent cells and monocytes were differentiated into macrophages for 3 days in RPMI media + 10% FBS containing 30 ng/ml M-CSF (PeproTech) and for further 4 days in media containing 30 ng/ml RANKL (PeproTech ...
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bioRxiv - Immunology 2022Quote: ... and resuspended in RPMI 1640 containing 20% FBS and 50 ng/ml human macrophage colony-stimulating factor (M-CSF) (300-25, Peprotech). Cells were seeded into sterile tissue-culture plates and differentiated for 7 days ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 5% heat-inactivated fetal bovine serum (Biosera FB-1001) and 50ng/ml recombinant M-CSF (Peprotech 300-25). Differentiated macrophages were plated at a density of 1 x 106ml in the appropriate cell culture plate at least 1 d prior to stimulation.
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bioRxiv - Cancer Biology 2023Quote: ... Cells were passed through 70-micron cell strainer and subsequently cultured 10mL complete RPMI media supplemented with 20 ng/mL of recombinant murine M-CSF (576406; BioLegend, 315-02 PeproTech). On day 3 ...
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bioRxiv - Microbiology 2023Quote: ... 1 x 106 BLaER1 cells per well of a 6-well tissue culture plate were treated with 10 ng/ml human recombinant M-CSF and IL-3 (PeproTech) and 100 nM β-estradiol (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... and cultured in complete RPMI 1640 media (10% fetal bovine serum, 1% penicillin-streptomycin, 1% L-glutamine) supplemented with 10 ng/mL M-CSF (PeproTech). Cells were cultured for 5 days at 37°C in a humidified 5% CO2 atmosphere under continuous high-dose 100 ng/mL lipopolysaccharide (LPS ...
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bioRxiv - Immunology 2023Quote: ... The transfection mix was then added dropwise to 2.5×106 M0 macrophages (pre-cultured for 6 days in a 6-well plate in antibiotic-free RPMI1640 macrophage media containing M-CSF (50ng/ml, Peprotech) – with media change on d3) ...
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bioRxiv - Immunology 2023Quote: CD11b+ mouse lung cells were seeded in 12-well dishes for 2 days in DMEM supplemented with M-Csf (315-02, Peprotech). Cells were stimulated with murine IL-6 for 72 hours ...
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bioRxiv - Immunology 2023Quote: ... bone marrow was taken from the tibias and femurs of 9-week-old C57BL/6 Lgals9 -/- mice or wild type litter mates and cultured in RPMI containing 10 % FBS and 3 % granulocyte macrophage colony-stimulating factor (mGM-CSF) (Peprotech). for 7 days ...
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bioRxiv - Immunology 2023Quote: ... BM cells isolated from WT C57BL/6 mice and Tollip−/− mice were cultured in complete RPMI 1640 medium supplemented with 10 ng/mL M-CSF (Peprotech) in the presence of 1 mM 4-PBA (MilliporeSigma) ...
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bioRxiv - Biochemistry 2022Quote: ... purified cells were cultured in complete medium added with 100 ng/ml macrophage colony-stimulating factor (M-CSF) (Peprotech, Stockholm, Sweden) or 100 ng/ml granulocyte–macrophage colony-stimulating factor (GM-CSF ...
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bioRxiv - Cell Biology 2020Quote: ... The monolayer was washed 3 times with HBSS to remove non-adherent cells and monocytes were differentiated for 3 days in RPMI media containing 20 ng/ml M-CSF and 20ng/ml recombinant human RANKL (PeproTech, UK). After 3 days of culture ...
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bioRxiv - Immunology 2022Quote: ... and differentiated for 7 days in Iscove’s Modified Dulbecco’s Media (IMDM) supplemented with 20 ng/ml of M-CSF (Peprotech, Rocky Hill, NJ), 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Immunology 2019Quote: ... Lin- bone marrow cells were grown in culture with complete IMDM medium supplemented with 10 ng/ml M-CSF (Peprotech, QC).
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bioRxiv - Cell Biology 2022Quote: ... were cultured at a ratio of red:green (1:1) in 8-well chamber slides (ThermoFischer Scientific, Cat # 154534) and stimulated with M-CSF (20 ng/ml, PeproTech, UK) for 3 days ...
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bioRxiv - Immunology 2022Quote: ... Whole mouse splenocytes and human PBMCs were plated at a density of 5×106 cells per well in a Cepallet W-type 24 well microplate (DIC, Japan) and cultured for 4 days with or without mouse/human M-CSF (Peprotech, USA). Surviving macrophages adhered to 24-well plates were detached by lowering the temperature on ice ...
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bioRxiv - Cancer Biology 2022Quote: ... PBMCs were harvested from K3EDTA anti-coagulated whole blood by density gradient centrifugation with Lymphoprep and plated in 12 well plates at 2-4 × 106 cells per well in 10% RPMI + 10 ng/mL human CSF (300-25, Peprotech, UK). Medium was replaced every 2-3 days until adherent populations of macrophages had established ...
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bioRxiv - Cell Biology 2023Quote: ... at a density of 0.15×106 cell/well and cultured for 2 days in supplemented α-MEM with 30 ng/ml M-CSF (PeproTech, UK). Media were replaced with α-MEM supplemented with 30 ng/ml M-CSF and 30 ng/ml RANKL (PeproTech ...
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The loss-of-function of SOCS2 increases the inflammatory response to Staphylococcus aureus infectionbioRxiv - Immunology 2023Quote: ... The cells were collected and cultured in X-Vivo (BE02-060F, Ozyme, France) supplemented with 50 ng/mL M-CSF (Peprotech, France) at a density of 2 x105 cells/mL ...
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bioRxiv - Cell Biology 2024Quote: ... Macrophage precursors were added to the NPC culture in 1:1 neural maintenance medium:microglia medium ((DMEM-F12+Glutamax, 100 ng/mL M-CSF (Peprotech, 300-25), 100 ng/mL IL-34 (Peprotech ...
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bioRxiv - Cell Biology 2022Quote: ... The cell suspension was washed with PBS and plated in DMEM with 10 ng mL-1 M-CSF (Peprotech Inc, 315-02). Following 5 days of culture ...
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bioRxiv - Systems Biology 2020Quote: ... 2 mM glutamine and 10% fetal calf serum in the presence of 50 ng/ml of M-CSF (Peprotech, cat# 300-25). 5 × 105 CD14+CD16− monocytes or × 105 resting macrophages were activated with 10 ng/ml of lipopolysaccharides (LPS ...
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bioRxiv - Cell Biology 2020Quote: ... cells were plated in 10 cm diameter Petri dishes with 10 ng/mL macrophage colony-stimulating factor (M-CSF, PeproTech, London, UK) for 24 h ...
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bioRxiv - Cell Biology 2021Quote: ... were flushed out and differentiated by culturing in DMEM-10% FCS supplemented with mouse colony stimulating factor (M-CSF, 25ng/ml, Peprotech, 315-02) for 5-6 days ...
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bioRxiv - Cancer Biology 2021Quote: ... 10% FBS) in the presence of macrophage colony stimulating factor (M-CSF) (30 ng/mL, #315-02, Peprotech, Rocky Hill, NJ, USA). After three days in culture ...
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bioRxiv - Cancer Biology 2021Quote: ... BMCs were cultured in DMEM/F-12 supplemented with 20 ng/mL murine M-CSF (315-02-10, Peprotech, Cranbury, NJ, USA) in a density of 4 × 106/mL ...
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bioRxiv - Immunology 2019Quote: ... Samples were adjusted to 7.5×105 cells/mL with cold cRPMI_M supplemented with 25 ng/mL of recombinant mouse colony-stimulating factor (m-csf, Peprotech, Rocky Hill, NJ) and cultured at 37°C ...
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bioRxiv - Bioengineering 2022Quote: ... macrophage colony stimulating factor (M-CSF) and receptor activator of nuclear factor kappa-B ligand (RANKL) were from PeproTech (London, United Kingdom). Bombyx mori L ...
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bioRxiv - Immunology 2023Quote: ... Bone marrow was plated into non-cell-culture treated 10cm petri dishes in 8mL supplemented RPMI medium containing either 20ng/mL recombinant M-CSF (Peprotech, #315-02) or 20ng/mL recombinant GM-CSF (Peprotech ...
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bioRxiv - Immunology 2023Quote: ... A total of 1.5×106/well CD14+ monocytes were seeded in a 12-well plate with 50 ng/mL of hM-CSF (Peprotech, 300-25). On day 6 ...
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bioRxiv - Cell Biology 2023Quote: ... BMMs were seeded at a density of 1.2 × 105 cells/well into 24-well culture plates and incubated in α-MEM (SH30265.01; Hyclone, Rockford, IL, USA) containing 20 ng/mL macrophage colony-stimulating factor (M-CSF) (300-25; PeproTech, Cranbury, NJ, USA). To induce osteoclast differentiation ...
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bioRxiv - Biochemistry 2019Quote: ... and 10 ng/ml rat TNF-α (Peprotech, 400-14) for 12 and 24 hours ...
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bioRxiv - Neuroscience 2023Quote: Some groups received recombinant rat CNTF (Peprotech; 200 ng/ml), either solely or combined with parthenolide ...
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bioRxiv - Microbiology 2021Quote: ... bone marrow cells were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS) in the presence of M-CSF (10 ng/ml, PeproTech, Cat# 315-02) for 7-9 days.
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bioRxiv - Cancer Biology 2021Quote: ... The cells were incubated in RPMI1640 for THP-1 culture supplemented with human M-CSF at 20 ng/ml (cross-react with mouse, Peprotech, Cranbury, NJ, USA). After 2 days of culture ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then cultured on untreated plates for 7 days adding 10 ng/ml recombinant murine M-CSF (Peprotech, AF-315-02-100) at day of isolation ...
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bioRxiv - Immunology 2019Quote: ... the cells were resuspended and cultured in macrophage-conditioned medium containing complete 1640 medium containing 10% (vol/vol) FBS and 100 U/ml penicillin and streptomycin and 20 ng/ml murine M-CSF (Peprotech, cat. # 315-02) for 7 days.
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bioRxiv - Cell Biology 2021Quote: ... For macrophage differentiation the bone marrow was resuspended in 25 ml of complete RPMI 1640 media supplemented with 20 ng/ml M-CSF (Peprotech #AF-315-02) and 50 ng/ml Amphotericin B ...
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bioRxiv - Molecular Biology 2021Quote: ... BMDM and AM were cultured in Dulbeccòs Modified Eagle’s Medium (DMEM) + 10% heat-inactivated fetal bovine serum (FBS) supplemented with 20 ng/ml recombinant murine M-CSF (PeproTech, Rocky Hill, NJ) and 20 ng/ml recombinant murine RANKL (PeproTech) ...
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bioRxiv - Immunology 2021Quote: ... Human monocyte-derived macrophages (HMDMs) were differentiated from purified CD14+ monocytes in the presence of recombinant human M-CSF (50 ng/ml; PeproTech, Cranbury, NJ, USA) for 6 days in RPMI 1640 medium supplemented with 10% FBS ...
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bioRxiv - Immunology 2020Quote: ... Isolated PBMC were washed with PBS and resuspended by RPMI-1640 which supplemented 50ng/ml recombinant human macrophage colony-stimulating factor (M-CSF) (AF-300-25-500, PeproTech, Cranbury, NJ, USA), then cultured at 37°C in 5% CO2 for 5 days to differentiate into human monocyte derived macrophages (hMDMs ...
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bioRxiv - Immunology 2022Quote: ... or CD11b+ enriched single cell suspensions of B16F10 tumors were cultured in the presence of 50 ng/mL recombinant M-CSF (Peprotech, Cranbury, NJ, USA) in RPMI (Wellgene ...
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bioRxiv - Immunology 2023Quote: ... were differentiated from PBMCs by culturing the cells in RPMI complete medium supplemented with 10% AB-positive human serum and 15 ng/ml M-CSF (PeproTech, 300-25-100UG). After 2 days of culturing ...