Labshake search
Citations for Peprotech :
501 - 550 of 794 citations for GM CSF Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: ... supplemented with 50 ng ml−1 of macrophage colony-stimulating factor (M-CSF, Peprotech, London, United Kingdom) (Murray 2014) ...
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bioRxiv - Immunology 2022Quote: ... and cells were plated at 5 × 105 cells/10 ml with 20 ng/ml M-CSF (PeproTech). On day 6 BMDM were replated at 1 × 106 cells per well in 24-well plates and stimulated with 10 µg/ml Imiquimod (Biolegend ...
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bioRxiv - Cancer Biology 2022Quote: ... with 10% fetal bovine serum (FBS; HyClone, SH30396.03) and 40 ng/ml M-CSF (Peprotech, 315-02). An additional 15 ml of media with M-CSF was added after day three ...
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bioRxiv - Biochemistry 2019Quote: ... 100 μg/ml streptomycin and 15 ng/ml recombinant human macrophage colony stimulating factor (hM-CSF) (Peprotech) on 10 mm square dishes (Sterilin) ...
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bioRxiv - Bioengineering 2023Quote: ... 10% fetal bovine serum (FBS) and 10 ng/mL murine-colony stimulating factor (M-CSF) (Peprotech, UK). Cells were cultured in non-treated cell culture dishes (Corning ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 5% heat-inactivated FCS (LabTech #80837) and 50 ng/ml M-CSF (PeproTech #300-25) for 7 days.
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bioRxiv - Molecular Biology 2024Quote: ... and plated at a density of 106 cells/ml supplemented with 20 ng/ml M-CSF (Peprotech). Cells were differentiated for 7 days ...
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bioRxiv - Pathology 2023Quote: ... we used the established in vivo Treg induction cocktail consisting of recombinant murine IL2 (0.01 ug/gm, Peprotech, Catalog 212-12) complexed to anti-IL2 antibody (0.05 ug/gm ...
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bioRxiv - Immunology 2022Quote: ... monocytes were treated for 7 days with 10 ng/mL human recombinant M-CSF (PeproTech, Rocky Hill, NJ) in R5 medium (RPMI 1640 (Gibco ...
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bioRxiv - Microbiology 2020Quote: ... Macrophages were generated from adherent cells in RPMI-1640 containing 10% FCS and M-CSF (Peprotech, 315-02) (30 ng/ml ...
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bioRxiv - Microbiology 2021Quote: ... Cells were pelleted and grown in complete RPMI 1640 media supplemented with 20 ng/ml mGM-CSF (Peprotech), antibiotics and 100 µM β-mercaptoethanol ...
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bioRxiv - Immunology 2019Quote: ... monocytes isolated by adherence were cultured in complete RPMI supplemented with 100ng/ml recombinant human M-CSF (Peprotech) in a 5% CO2 at 37°C for 7 days ...
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bioRxiv - Neuroscience 2022Quote: ... (2018) with modified maturation supplementation using 25 ng/mL hM-CSF and 100 ng/mL hIL-34 (PeproTech) (McQuade et al. ...
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bioRxiv - Bioengineering 2022Quote: ... 10 mM β-glycerophosphate) spiked with 50 ng/mL macrophage colony stimulating factor (M-CSF, 300-25, PeproTech). The cells were counted and 1.9 × 106 monocytes were resuspended in 20 μL coculture medium and per scaffold added on top of the scaffolds that had previously been seeded with MSCs as described above (chapter 2.4) ...
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bioRxiv - Bioengineering 2023Quote: ... 10 mM β-glycerophosphate) spiked with 50 ng/mL macrophage colony stimulating factor (M-CSF, 300-25, PeproTech). After 21 days of culturing the MSCs ...
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bioRxiv - Immunology 2023Quote: ... Cells were centrifugated and resuspended in media containing 10 ng/mL macrophage-colony stimulating factor (M-CSF) (Peprotech), and plated on 10-cm untreated petri dishes per mouse in 15 mL of media and cultured for 7 days without replenishing or changing media to derive BMDMs ...
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bioRxiv - Molecular Biology 2023Quote: ... macrophages were generated from adherent cells in RPMI-1640 containing 10% FCS and M-CSF (Peprotech, 315-02) (30 ng/ml ...
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bioRxiv - Bioengineering 2024Quote: ... 20 ng/mL interleukin-4 (IL-4) and 50 ng/mL recombinant human M-CSF (Peprotech EC Ltd.) were added to the culture media for 24 hours ...
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bioRxiv - Cancer Biology 2024Quote: ... Macrophages were generated from this CD14-positive population by activation with M-CSF (25 ng/ml, PeproTech, USA) over a 7-day period ...
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bioRxiv - Immunology 2021Quote: ... pooled plasma or depleted plasma from the study groups of interest were pretreated with anti-G-CSF Ab (0.2 μg/ml, Peprotech) for 30 minutes.
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bioRxiv - Molecular Biology 2020Quote: ... supplemented with 20% FBS and 10 ng/μL macrophage colony-stimulating factor (M-CSF) (PeproTech, Inc., Rocky Hill, NJ). Cells were passed through a 70 μm filter to clear any debris ...
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bioRxiv - Genetics 2022Quote: ... supplemented with 20% FBS and 10 ng/μL macrophage colony-stimulating factor (M-CSF) (PeproTech, Inc., Rocky Hill, NJ). Cells were passed through a 70 μm filter to clear debris ...
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bioRxiv - Neuroscience 2021Quote: ... and the pellet resuspended in 12 mL of DMEM media containing 10 ng/mL recombinant murine M-CSF (Peprotech). Cell suspensions were then pooled as required and placed in a 100 mm x 20 mm (diameter x height ...
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bioRxiv - Cell Biology 2022Quote: ... Monocytes (3.6 ×106 cells) were first seeded and stimulated with M-CSF for 3 days (20 ng/ml, PeproTech) in 1-well chamber slides (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Media were replaced with α-MEM supplemented with 30 ng/ml M-CSF and 30 ng/ml RANKL (PeproTech) for 3 days ...
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bioRxiv - Molecular Biology 2023Quote: ... and then differentiated the cells into macrophages by supplementing with M-CSF (20 ng/mL, Peprotech, Rocky Hill, NJ). Seahorse stress testing was performed to compare their metabolic glycolytic activity after 7 days of differentiation.
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bioRxiv - Cell Biology 2023Quote: ... received in addition to the base medium 50 ng/ml macrophage colony-stimulating factor (M-CSF, 300-25, PeproTech) and 50 ng/ml receptor activator of nuclear factor κB ligand (RANKL ...
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bioRxiv - Biophysics 2021Quote: ... the medium was replaced by RPMI containing 10% FCS and 20 ng/mL of Macrophage Colony-Stimulating Factor (M-CSF) (Peprotech). For experiments ...
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FOXO dictate initiation of B cell development and myeloid restriction in common lymphoid progenitorsbioRxiv - Immunology 2022Quote: ... 25ng/ml human FL and 10ng/ml murine IL3 with or without 25ng/ml murine M-CSF (all from PeproTech). No apparent difference was observed between cultures with or without M-CSF and data was combined ...
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bioRxiv - Immunology 2019Quote: Bone marrow-derived macrophages (BMDMs) were cultured in complete RPMI with recombinant human CSF-1 (50 ng/ml; PeproTech Inc.) for 6 days ...
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bioRxiv - Neuroscience 2021Quote: ... 1% penicillin/streptomycin) was changed every 3-5 days and M-CSF was supplemented (Peprotech 315-02, 5 ng/mL) for 2 weeks ...
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bioRxiv - Neuroscience 2021Quote: ... in the presence of 30 ng/mL M-CSF and 100 ng/mL receptor activator of nuclear factor kappa-B ligand (RANKL, PeproTech) (Sousa et al. ...
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bioRxiv - Neuroscience 2021Quote: ... Pre-osteoclasts were obtained after 3 days of stimulation with macrophage colony-stimulating factor (M-CSF 30 ng/mL, PeproTech). Adherent cells were then detached and seeded on top of bone slices (boneslices.com ...
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bioRxiv - Bioengineering 2022Quote: ... MCs were first cultured in priming medium (osteoclast control medium + 50 ng/ml macrophage colony-stimulating factor (M-CSF, 300-25, PeproTech)) ...
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bioRxiv - Microbiology 2022Quote: ... 1 × 106 BlaER1 cells per well of a 6-well tissue culture plate were treated with 10 ng/ml human recombinant M-CSF and IL-3 (PeproTech) and 100 nM β-estradiol (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 ng/mL IL-3 (Cat# 200-03) and 10 ng/mL G-CSF (Cat# 300-23; all cytokines from Peprotech), 100 IU/mL penicillin ...
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bioRxiv - Immunology 2020Quote: ... 100 U ml−1 penicillin-streptomycin and 10% FCS) with 20 ng ml−1 murine macrophage colony-stimulating factor 1 (CSF-1; Peprotech) for 7 days ...
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bioRxiv - Molecular Biology 2022Quote: ... 150 ng/ml Flt-3, PeproTech, Cat 300-19; 10 ng/ml IL-6, PeproTech, Cat 200-06; 25 ng/ml G-CSF, PeproTech Cat 300-23 ...
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bioRxiv - Immunology 2022Quote: ... 100 U ml−1 penicillin-streptomycin and 10% FCS) with 20 ng ml−1 murine macrophage colony-stimulating factor 1 (CSF-1; Peprotech) for 7 days ...
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bioRxiv - Cell Biology 2019Quote: ... and then cultured for 3 days with 40% dilution (vol/vol) of cmCTR or cmMTB supplemented with 20% FBS and M-CSF (20 ng/mL, Peprotech). Blocking IFNAR receptor was performed by pre-incubation with mouse anti-IFNAR antibody (20 μg/mL ...
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bioRxiv - Cell Biology 2019Quote: ... monocytes were also conditioned in presence of 20 ng/mL M-CSF and 10 ng/mL recombinant human IL-10 (PeproTech) or 10 U/mL of IFNβ (Peprotech) ...
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bioRxiv - Cancer Biology 2019Quote: Primary murine macrophages were generated by flushing the bone marrow from the femur and tibia of 6-8 week old C57BL/6 mice followed by incubation for 5 days in DMEM containing 10% FBS and 10 ng/mL murine M-CSF (Peprotech). Primary pancreatic stellate cells were isolated from the pancreas of C57BL/6 mice by density gradient centrifugation ...
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bioRxiv - Immunology 2019Quote: Human CD14+ monocytes were purified from leukocyte preparations purchased from the New York Blood Center and differentiated into blood-derived macrophages for 1–2 d in 10 ng/ml M-CSF (PeproTech) and RPMI 1640 medium (Life Technologies)/10% defined FBS (Hyclone) ...
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bioRxiv - Immunology 2020Quote: ... the non-adherent cells were transferred to a new petri dish and incubated with BMDM media + 20 ng/ml macrophage-colony stimulating factor (M-CSF; Peprotech). After 3 days ...
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bioRxiv - Cancer Biology 2021Quote: ... bone marrow cells were obtained from 8-10 weeks old BALB/c mice and cultured for 7 days in DMEM supplemented with 10% FBS and 20 μg/ml macrophage-colony stimulating factor (M-CSF, Peprotech). All medium contained 50 U/ml penicillin and 50 μg/ml streptomycin (Sigma-Aldrich).
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bioRxiv - Immunology 2019Quote: ... 50μM 2-ME and 100ng/ml recombinant murine macrophage colony stimulating factor (M-CSF, PeproTech Inc., Rocky Hill, NJ, USA) for 6 days ...
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bioRxiv - Genetics 2019Quote: ... monocytes were plated in a 100mm x 20mm dish and cells were treated with 800 U/ml M-CSF (PeproTech) for seven days ...
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bioRxiv - Neuroscience 2021Quote: ... In the absence of β-estradiol and the presence of 20 ng/mL Macrophage Colony-Stimulating Factor (M-CSF; Peprotech) MØP cells differentiate into cells phenotypically similar to bone marrow-derived macrophages22.
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bioRxiv - Immunology 2020Quote: Adherent PBMC monolayer was washed twice with HBSS and monocytes were differentiated into hMDMs for 5 days in RPMI 1640 containing 100 ng/mL macrophage colony-stimulating factor (M-CSF, PeproTech) and 10% foetal calf serum ...
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bioRxiv - Cell Biology 2020Quote: ... Adherent cells were then detached with a cell scrapper and seeded at a density of 5 × 104 cells/cm2 in the presence of 30 ng/mL M-CSF and 100 ng/mL receptor activator of nuclear factor kappa-B ligand (RANKL, PeproTech) (Bartell et al. ...