Labshake search
Citations for Peprotech :
501 - 550 of 790 citations for G CSF Mouse CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2021Quote: ... bone marrow cells were obtained from 8-10 weeks old BALB/c mice and cultured for 7 days in DMEM supplemented with 10% FBS and 20 μg/ml macrophage-colony stimulating factor (M-CSF, Peprotech). All medium contained 50 U/ml penicillin and 50 μg/ml streptomycin (Sigma-Aldrich).
-
bioRxiv - Immunology 2019Quote: ... 50μM 2-ME and 100ng/ml recombinant murine macrophage colony stimulating factor (M-CSF, PeproTech Inc., Rocky Hill, NJ, USA) for 6 days ...
-
bioRxiv - Genetics 2019Quote: ... monocytes were plated in a 100mm x 20mm dish and cells were treated with 800 U/ml M-CSF (PeproTech) for seven days ...
-
bioRxiv - Microbiology 2019Quote: ... Purified CD14+ monocytes were cultured in complete RPMI supplemented with 100ng/mL each of recombinant human IL-4 and GM-CSF (PeproTech) at a cell density of 2e6 cells/mL ...
-
bioRxiv - Neuroscience 2021Quote: ... In the absence of β-estradiol and the presence of 20 ng/mL Macrophage Colony-Stimulating Factor (M-CSF; Peprotech) MØP cells differentiate into cells phenotypically similar to bone marrow-derived macrophages22.
-
bioRxiv - Immunology 2020Quote: Adherent PBMC monolayer was washed twice with HBSS and monocytes were differentiated into hMDMs for 5 days in RPMI 1640 containing 100 ng/mL macrophage colony-stimulating factor (M-CSF, PeproTech) and 10% foetal calf serum ...
-
bioRxiv - Cell Biology 2020Quote: ... Adherent cells were then detached with a cell scrapper and seeded at a density of 5 × 104 cells/cm2 in the presence of 30 ng/mL M-CSF and 100 ng/mL receptor activator of nuclear factor kappa-B ligand (RANKL, PeproTech) (Bartell et al. ...
-
bioRxiv - Cell Biology 2020Quote: ... After 2 days of culture with 20 ng/ml M-CSF and 20 ng/ml recombinant human RANKL (PeproTech, UK), the wells were rinsed twice with PBS and incubated with 10% bleach solution for 30 min at room temperature ...
-
bioRxiv - Immunology 2021Quote: ... The lavage was RBC-lysed and resuspended in RPMI medium containing 10 ng/ml murine M-CSF (Peprotech 315-02) for co-culture with CAR or control transduced T cells.
-
bioRxiv - Immunology 2021Quote: The differentiation of isolated monocytes into macrophages was performed in RPMI 1640 GlutaMAx medium with 10% FCS and 1% P/S containing 100 ng/mL macrophage colony-stimulating factor (M-CSF) (PeproTech) for 7 days ...
-
bioRxiv - Immunology 2020Quote: ... the non-adherent cells were transferred to a new non-tissue culture treated petri dish and incubated with BMDM media supplemented with 20 ng/mL M-CSF (PeproTech). On day 3 after plating ...
-
bioRxiv - Immunology 2020Quote: ... These cells were cultured for 7 d in DMEM cell culture medium containing 40 ng/ml macrophage colony-stimulating factor (M-CSF) (PeproTech). For the generation of bone marrow-derived dendritic cells ...
-
bioRxiv - Immunology 2020Quote: ... in the presence of 50 ng/ml granulocyte-macrophage colony-stimulating factor (GM-CSF) and of 20 ng/ml interleukin-4 (IL-4) (PeproTech). DCs stimulation was performed 6 days post-isolation of monocytes ...
-
bioRxiv - Molecular Biology 2021Quote: ... with subsequent maintenance of adherent cells in RPMI 1640 medium supplemented with 10% human serum and M-CSF (10 ng/ml, Peprotech) for 3 days and then differentiated for a further 4 days in RPMI 1640 medium supplemented with 10% fetal calf sera without M-CSF.
-
bioRxiv - Immunology 2020Quote: ... approximately 2 x 106 cells were then seeded per 100 mm plate in 10 ml of media containing 20 ng/ml of recombinant murine (rm) GM-CSF (Peprotech). On day 3 ...
-
bioRxiv - Cell Biology 2021Quote: ... bone marrows were cultured and in vitro differentiated in bone marrow differentiation medium containing 40 ng/ml recombinant murine M-CSF (PeproTech) as described before (Sun et al. ...
-
bioRxiv - Immunology 2022Quote: ... bone marrow cells harvested from femurs and tibias of 6–10-week-old control or EROS-/- mice were grown in complete RPMI medium supplemented with 100 ng/mL of M-CSF (Peprotech) for 3 days ...
-
bioRxiv - Cell Biology 2022Quote: ... The monolayer was washed 3 times with HBSS to remove non-adherent cells and monocytes were differentiated into macrophages for 3 days in RPMI media + 10% FBS containing 30 ng/ml M-CSF (PeproTech) and for further 4 days in media containing 30 ng/ml RANKL (PeproTech ...
-
bioRxiv - Immunology 2022Quote: ... and resuspended in RPMI 1640 containing 20% FBS and 50 ng/ml human macrophage colony-stimulating factor (M-CSF) (300-25, Peprotech). Cells were seeded into sterile tissue-culture plates and differentiated for 7 days ...
-
bioRxiv - Microbiology 2022Quote: ... Cells were cultured in RPMI 1640 medium with 10% FBS containing GM-CSF (25 ng/ml; Peprotech, Rocky Hill, NJ) and IL-4 (10 ng/ml ...
-
bioRxiv - Immunology 2023Quote: ... supplemented with 5% heat-inactivated fetal bovine serum (Biosera FB-1001) and 50ng/ml recombinant M-CSF (Peprotech 300-25). Differentiated macrophages were plated at a density of 1 x 106ml in the appropriate cell culture plate at least 1 d prior to stimulation.
-
bioRxiv - Microbiology 2023Quote: ... 1 x 106 BLaER1 cells per well of a 6-well tissue culture plate were treated with 10 ng/ml human recombinant M-CSF and IL-3 (PeproTech) and 100 nM β-estradiol (Sigma-Aldrich ...
-
bioRxiv - Immunology 2023Quote: ... and cultured in complete RPMI 1640 media (10% fetal bovine serum, 1% penicillin-streptomycin, 1% L-glutamine) supplemented with 10 ng/mL M-CSF (PeproTech). Cells were cultured for 5 days at 37°C in a humidified 5% CO2 atmosphere under continuous high-dose 100 ng/mL lipopolysaccharide (LPS ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cells were passed through 70-micron cell strainer and subsequently cultured 10mL complete RPMI media supplemented with 20 ng/mL of recombinant murine M-CSF (576406; BioLegend, 315-02 PeproTech). On day 3 ...
-
bioRxiv - Immunology 2023Quote: ... The transfection mix was then added dropwise to 2.5×106 M0 macrophages (pre-cultured for 6 days in a 6-well plate in antibiotic-free RPMI1640 macrophage media containing M-CSF (50ng/ml, Peprotech) – with media change on d3) ...
-
bioRxiv - Immunology 2023Quote: ... HeLa-sia) at a ratio of 1:100 in complete RPMI medium supplemented with 10 ng/mL GM-CSF (PeproTech). Cancer cells were seeded at an initial concentration of 1×10e4 cells/mL and the same amount of medium supplemented with GM-CSF was added at day 4 of the experiment ...
-
bioRxiv - Immunology 2023Quote: ... bone marrow was taken from the tibias and femurs of 9-week-old C57BL/6 Lgals9 -/- mice or wild type litter mates and cultured in RPMI containing 10 % FBS and 3 % granulocyte macrophage colony-stimulating factor (mGM-CSF) (Peprotech). for 7 days ...
-
bioRxiv - Cancer Biology 2023Quote: ... and conditioned media containing approximately 100 ng/ml SCF for the generation of neutrophil-biased cells or GM-CSF 10 ng/ml (Peprotech) for the generation of macrophage progenitors ...
-
bioRxiv - Immunology 2023Quote: ... BM cells isolated from WT C57BL/6 mice and Tollip−/− mice were cultured in complete RPMI 1640 medium supplemented with 10 ng/mL M-CSF (Peprotech) in the presence of 1 mM 4-PBA (MilliporeSigma) ...
-
bioRxiv - Immunology 2023Quote: ... 3×105 CD14+ monocytes were seeded in a 12-well plate in 1 mL complete medium containing 20 ng/mL GM-CSF (#300-03; Peprotech) or 50 ng/mL M-CSF (#300-25 ...
-
bioRxiv - Immunology 2023Quote: CD11b+ mouse lung cells were seeded in 12-well dishes for 2 days in DMEM supplemented with M-Csf (315-02, Peprotech). Cells were stimulated with murine IL-6 for 72 hours ...
-
bioRxiv - Immunology 2023Quote: ... Bone marrow-derived dendritic cells (BMDCs) were generated in tissue culture using recombinant GM-CSF (Peprotech, Cat. AF-315-03). Class B CpG 1826 oligonucleotide was synthesized by Integrated DNA Technologies ...
-
bioRxiv - Cancer Biology 2023Quote: ... Differentiation of BM cells into DCs was carried out in low attachment 10 mm cell culture dish in presence of bone marrow-differentiation media in presence of recombinant murine Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) (Cat. 315-03, Peprotech) for 48 a ...
-
bioRxiv - Immunology 2024Quote: ... CD14+ monocytes were isolated from PBMCs using the EasySep Human CD14 Positive Selection Kit (Stem Cell) and cultured with 100U/mL GM-CSF (Peprotech) for 4 days prior to plating for stimulation ...
-
bioRxiv - Immunology 2023Quote: ... filtered and cultured for 7 days in complete RPMI medium (RPMI-1640 medium containing L-glutamine plus 10% fetal calf serum) containing 20ng/mL recombinant GM-CSF (Peprotech).
-
bioRxiv - Bioengineering 2024Quote: ... Cells were then filtered through 40 μm strainer and resuspended with complete RPMI supplemented with 25ng/ml murine GM-CSF (Peprotech) to a final concentration of 2 million cells/ml and plated in 24-well plates.
-
bioRxiv - Cell Biology 2021Quote: ... purified and cultured with 10% FBS containing GM-CSF (10 ng/mL) and IL 4 (10 ng/mL) (all PeproTech, USA). FBS (10% ...
-
bioRxiv - Biochemistry 2022Quote: ... purified cells were cultured in complete medium added with 100 ng/ml macrophage colony-stimulating factor (M-CSF) (Peprotech, Stockholm, Sweden) or 100 ng/ml granulocyte–macrophage colony-stimulating factor (GM-CSF ...
-
bioRxiv - Cell Biology 2020Quote: ... The monolayer was washed 3 times with HBSS to remove non-adherent cells and monocytes were differentiated for 3 days in RPMI media containing 20 ng/ml M-CSF and 20ng/ml recombinant human RANKL (PeproTech, UK). After 3 days of culture ...
-
bioRxiv - Immunology 2022Quote: ... and differentiated for 7 days in Iscove’s Modified Dulbecco’s Media (IMDM) supplemented with 20 ng/ml of M-CSF (Peprotech, Rocky Hill, NJ), 10% heat-inactivated fetal bovine serum (FBS ...
-
bioRxiv - Immunology 2022Quote: ... X-Vivo 15 media was supplemented with 100 ng/ml human granulocyte-macrophage colony-stimulating factor (GM-CSF) and 20 ng/ml human interleukin 4 (IL-4) (both cytokines obtained from Peprotech, Germany), and cells were cultured with 5% CO2 ...
-
bioRxiv - Immunology 2019Quote: ... 1ml of cell suspension was plated per well in tissue culture-treated 24-well plates and supplemented with 20ng/ml recombinant GM-CSF and 5ng/ml TGFβ (Peprotech, USA). Cells were cultured at 37°C ...
-
bioRxiv - Microbiology 2021Quote: ... and fresh 10% fetal bovine serum (10% FBS) with GM-CSF (10 ng/mL) and IL-4 (10 ng/mL) (all PeproTech, USA) were added ...
-
bioRxiv - Immunology 2019Quote: ... bone marrow cells were plated at a density of 5×105 cells/mL in RPMI-1640 + 10% FCS + 100 U/mL penicillin/streptomycin + 1 mM β-mercaptoethanol (BM medium) with 20 ng/mL murine GM-CSF (Peprotech) and cultures in a humidified environment at 37°C and 5% CO2 ...
-
bioRxiv - Immunology 2019Quote: ... Lin- bone marrow cells were grown in culture with complete IMDM medium supplemented with 10 ng/ml M-CSF (Peprotech, QC).
-
bioRxiv - Immunology 2021Quote: ... then the remaining negatively sorted cells were resuspended at 5×105 cells/ml in complete Iscove’s modified Dulbecco’ s medium supplemented with Granulocyte-macrophage colony-stimulating factor (GM-CSF) (PeproTech, #315-03), FLT-3L (PeproTech ...
-
bioRxiv - Cell Biology 2022Quote: ... were cultured at a ratio of red:green (1:1) in 8-well chamber slides (ThermoFischer Scientific, Cat # 154534) and stimulated with M-CSF (20 ng/ml, PeproTech, UK) for 3 days ...
-
bioRxiv - Cancer Biology 2022Quote: ... PBMCs were harvested from K3EDTA anti-coagulated whole blood by density gradient centrifugation with Lymphoprep and plated in 12 well plates at 2-4 × 106 cells per well in 10% RPMI + 10 ng/mL human CSF (300-25, Peprotech, UK). Medium was replaced every 2-3 days until adherent populations of macrophages had established ...
-
The loss-of-function of SOCS2 increases the inflammatory response to Staphylococcus aureus infectionbioRxiv - Immunology 2023Quote: ... The cells were collected and cultured in X-Vivo (BE02-060F, Ozyme, France) supplemented with 50 ng/mL M-CSF (Peprotech, France) at a density of 2 x105 cells/mL ...
-
bioRxiv - Cell Biology 2023Quote: ... at a density of 0.15×106 cell/well and cultured for 2 days in supplemented α-MEM with 30 ng/ml M-CSF (PeproTech, UK). Media were replaced with α-MEM supplemented with 30 ng/ml M-CSF and 30 ng/ml RANKL (PeproTech ...