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Citations for Peprotech :
351 - 400 of 474 citations for DNA Damage 8 OHdG ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... embryoid bodies are transferred to adherent conditions on Matrigel-coated plates and medium was supplemented with 25 ng/ml VEGF-A (Peprotech, 100-20 ...
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bioRxiv - Immunology 2020Quote: ... and were then cultured at 1-2 x 106/ml in 12-well plates with differentiation media containing 50 ng/ml Interleukin 4 (Peprotech) and 100 ng/ml granulocyte-monocyte colony stimulating factor (GM-CSF ...
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bioRxiv - Immunology 2021Quote: ... Forty-eight hours later cells were lifted off the plates and cultures were supplemented with 100 U/ml recombinant human IL-2 (Peprotech). CFSE labeling for certain experiments was performed prior to seeding on tissue culture plates.
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The skin environment controls local dendritic cell differentiation and function through innate IL-13bioRxiv - Immunology 2021Quote: ... were harvested and one million cells were resuspended in 1 mL of TCM in 24 wells plates and stimulated with recombinant mouse IL-13 (Peprotech) at a concentration of 100 ng/mL for 30 min at 37°C ...
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bioRxiv - Immunology 2020Quote: ... approximately 2 x 106 cells were then seeded per 100 mm plate in 10 ml of media containing 20 ng/ml of recombinant murine (rm) GM-CSF (Peprotech). On day 3 ...
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The logic of native enhancer-promoter compatibility and cell-type-specific gene expression variationbioRxiv - Genomics 2022Quote: ... and seeded to 0.1% gelatinized tissue culture plates in N2B27 supplemented with 10ng/ml FGF2 and 10 ng/ml Epithelial growth factor (EGF) (PeproTech). All cell lines were passaged regularly (every 2 to 4 days ...
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bioRxiv - Microbiology 2023Quote: ... 1 x 106 BLaER1 cells per well of a 6-well tissue culture plate were treated with 10 ng/ml human recombinant M-CSF and IL-3 (PeproTech) and 100 nM β-estradiol (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... 4-6 x 106 P14 CD8 T cells were plated in 6 well plates and pre-cultured in IL-7 (10ng/ml, PeproTech) at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... and plated onto 3 wells of a 6-well plate precoated with GFR Matrigel in N2B27 with 100ng/ml FGFb (100-18B, Peprotech) and 100ng/ml EGF (AF-100-15 ...
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bioRxiv - Immunology 2023Quote: ... The transfection mix was then added dropwise to 2.5×106 M0 macrophages (pre-cultured for 6 days in a 6-well plate in antibiotic-free RPMI1640 macrophage media containing M-CSF (50ng/ml, Peprotech) – with media change on d3) ...
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bioRxiv - Genetics 2023Quote: ... and seeded on a poly-D-lysine/laminin plate with N2B27 media supplemented with BDNF and GDNF (10 ng/ml each; Peprotech). Media were changed the next day to facilitate neuronal maturation and survival ...
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bioRxiv - Immunology 2023Quote: ... were cultured in six-well plates (2 ml per well) in complete RPMI 1640 medium + 10% FBS + 10 ng/ml human IL-4 (PeproTech) + 100 ng/ml human GM-CSF (PeproTech) ...
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bioRxiv - Neuroscience 2023Quote: ... Media was changed every 24h up to day 10 on which cells were mechanically clump passaged using 0.02% EDTA in PBS onto fibronectin coated plates and maintained in the same media without SMAD inhibitors and including 25ng/ml activin-A (Peprotech). All subsequent media changes were half volumes and every 48h before passaging again onto poly-l-ornithine and laminin coated 12w plates on day 20 ...
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bioRxiv - Immunology 2023Quote: ... 3×105 CD14+ monocytes were seeded in a 12-well plate in 1 mL complete medium containing 20 ng/mL GM-CSF (#300-03; Peprotech) or 50 ng/mL M-CSF (#300-25 ...
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bioRxiv - Genetics 2023Quote: ... An aliquot of 500,000 cells were plated in 200 µL of media in a 96-well plate and stimulated with 20 ng/mL of IL-6 (Peprotech) for 15 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... 1x106 cells were plated per well of 96-well round bottom plate and treated with 10ng/mL of TGF-β1 (Peprotech) or equivalent volume of DMSO for 20 minutes at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... cells were plated (60,000 cells/well in 24-well plates) two days before treatment in complete media supplemented with 25 ng/mL murine IL-4 (Peprotech) for M2-polarization or without any cytokines (M0) ...
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bioRxiv - Immunology 2023Quote: ... 2×105 BMDMs were seeded into 24-well plates and cultured for 24 h in the presence of 50 ng/mL IFNγ (Peprotech) and 100-1000 U/mL IL2 (Proleukin S ...
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bioRxiv - Developmental Biology 2021Quote: ... FACS-isolated Flk1+ mesodermal cells were cultured at 5 x104 cells/cm2 in SP34 plus SCF (Peprotech) and VEGF (R&D) ...
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bioRxiv - Immunology 2022Quote: ... and cells were plated at 5 × 105 cells/10 ml with 20 ng/ml M-CSF (PeproTech). On day 6 BMDM were replated at 1 × 106 cells per well in 24-well plates and stimulated with 10 µg/ml Imiquimod (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... and gentamycin supplemented with Flt3 (5 ng/ml; R&D) and IL-7 (1 ng/ml; Peprotech). For the scRNA-seq experiments ...
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bioRxiv - Cancer Biology 2022Quote: ... Non-enriched cells were cultured and expanded in TCM supplemented with 5 ng/mL IL-7 (Peprotech) and 5 ng/mL IL-15 (Peprotech ...
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bioRxiv - Cancer Biology 2023Quote: ... After 6 hours of serum starvation cells were stimulated with recombinant mouse LIF (5 ng/mL, Peprotech). Cells were lysed with Pierce RIPA buffer (Thermo Scientific ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 5% heat-inactivated FCS (LabTech #80837) and 50 ng/ml M-CSF (PeproTech #300-25) for 7 days.
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bioRxiv - Genomics 2019Quote: Day 24 G0 and G1 kidney organoids in identically plated wells of a 24-well plate were treated with interferon-gamma (IFN-γ, 25ng/mL, PeproTech) for 24 hours to induce APOL1 expression ...
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bioRxiv - Cell Biology 2022Quote: HUVECs were transfected 48 h before the assay and transferred to gelatin-coated black-walled 96-well plates (Falcon) and stimulated with TNF-α (10 ng/ml; Peprotech) or PBS (Gibco ...
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bioRxiv - Immunology 2019Quote: ... 1ml of cell suspension was plated per well in tissue culture-treated 24-well plates and supplemented with 20ng/ml recombinant GM-CSF and 5ng/ml TGFβ (Peprotech, USA). Cells were cultured at 37°C ...
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bioRxiv - Immunology 2020Quote: PBMCs were plated in 24-well plates at 2×106 cells per well with 100 IU/ml IL-2 (PeproTech, USA) in the complete medium ...
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bioRxiv - Cancer Biology 2022Quote: ... were plated onto 24-well plates in Matrigel and cultured for 3 days before treatment with either recombinant murine INF-y (PeproTech, UK) at 1 ng/mL or recombinant murine TGFβ 1 (R&D ...
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bioRxiv - Cancer Biology 2022Quote: ... PBMCs were harvested from K3EDTA anti-coagulated whole blood by density gradient centrifugation with Lymphoprep and plated in 12 well plates at 2-4 × 106 cells per well in 10% RPMI + 10 ng/mL human CSF (300-25, Peprotech, UK). Medium was replaced every 2-3 days until adherent populations of macrophages had established ...
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bioRxiv - Immunology 2024Quote: ... Transduced cells were incubated for 4 days with plate-bound anti-CD3/anti-CD28 in media supplemented with hIL-2 (0.0344 units/mL, Peprotech #200-02). Transduction efficiency was assessed for each experiment by flow cytometry.
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bioRxiv - Immunology 2021Quote: ... in antibiotic-free RPMI-1640 medium supplemented with 10% FBS and murine IL-5 (10 ng/mL, PeproTech). 108 bioluminescent C ...
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bioRxiv - Immunology 2019Quote: ... Dendritic cells (DCs) were generated by culturing monocytes for 5 days with IL-4 and GM-CSF (Peprotech). Immature DCs were harvested on day 5 of culture ...
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bioRxiv - Bioengineering 2019Quote: ... in mouse embryonic fibroblast (MEF)-conditioned media supplemented with 5 ng/ml basic fibroblast growth factor (bFGF) (Peprotech). Cardiomyocyte differentiation was performed as described previously using a combination of small molecules and growth factors 5 ...
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bioRxiv - Immunology 2019Quote: ... the PSC were resuspended at 100,000 cells/ml in the BDM supplemented with 5 ng/ml BMP4 (Peprotech) and plated at 20 ul/drop for inverted culture in 15 cm dishes ...
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bioRxiv - Cancer Biology 2020Quote: ... MVNs within large devices were treated with the enzymes or TNFα (5 ng mL-1, 300-01A, Peprotech) and fixed after six hours ...
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bioRxiv - Immunology 2021Quote: ... the PSCs were resuspended at 100,000 cells/mL in the BDM supplemented with 5 ng/mL BMP4 (Peprotech) and plated at 20 uL/drop for inverted culture in 15 cm dishes ...
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bioRxiv - Cell Biology 2022Quote: ... stem cell factor (SCF; 5% supernatant of murine SCF-secreting CHO transfectants) and IL4 (1 ng/ml, PeproTech) were added to the medium 25 ...
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bioRxiv - Immunology 2022Quote: ... and cultured at 37 °C in 5% CO2 with the indicated amounts of recombinant mouse IL-15 (PeproTech).
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bioRxiv - Immunology 2023Quote: ... The NK cells were counted and were resuspended in cRPMI supplemented with 5 ng/ml rhIL-15 (PeproTech) and rested overnight in a 96-well U-bottom plate at 37 °C and 5 % CO2.
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bioRxiv - Immunology 2023Quote: ... were incubated for 24 hours in RPMI-10 supplemented with IL-7 (5 ng/mL, Peprotech 217-17). T cells were then rinsed with PBS and 10 × 106 cells were resuspended in 20 µl of P4 primary cell nucleofection solution (P4 Primary Cell 4D-Nucleofector X Kit S ...
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bioRxiv - Cell Biology 2024Quote: ... MSCs were treated with 1 ng/mL or 5 ng/mL recombinant murine TNFα (PeproTech; cat. #315-01A), 50 ng/mL recombinant IL-6 (StemCell Technologies cat ...
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bioRxiv - Bioengineering 2021Quote: ... The specimens with the attached PBMCs were subsequently placed into 24 well plates and then differentiated by the addition of 20 ng/mL RANKL (Peprotech 310-01) and 10 ng/mL M-CSF (R&D ...
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bioRxiv - Cell Biology 2022Quote: ... cells were lifted using accutase and plated onto Laminin-111/ poly-L-ornithine-coated plates in NIM supplemented with 10 ng/ml BDNF (Peprotech, 450-02) and 10 ng/ml GDNF (Peprotech ...
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bioRxiv - Neuroscience 2020Quote: For the ADV transduction experiments human iPSC derived NSCs were plated at 700,000 cells per well of a 6-well plate in 2 ml NPM supplemented with 25 ng/ml βFGF (Peprotech, 100-18B) and 25 ng/ml Activin A (Peprotech ...
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bioRxiv - Pathology 2020Quote: ... HUVEC were plated at a density of 10,000 cells/96-well plate in EGM-2 supplemented with recombinant human TNF-α (300-01A, PeproTech EC Ltd), IL1-β (200-01B ...
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bioRxiv - Cell Biology 2022Quote: ... After 24 hours cells were re-plated at 30,000 cells/ml in gelatinized 24-well plates in DMEM complete medium supplemented with IL-3 (Peprotech, Cat#213-13) mCSF-1 (Peprotech ...
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bioRxiv - Immunology 2023Quote: Jurkat and THP1 WT and ARPC5 KO cells were seeded in 96-well plate and stimulated with RPMI 1640 + 100 ng/ml CXCL12 (Peprotech, 300-28A) or RPMI 1640 for the indicated time points ...
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bioRxiv - Immunology 2023Quote: ... TIL or KSQ-001 cells were added to spheroid plate in 100μL REP media supplemented with 6000IU/mL IL-2 (Peprotech, Cat#200-02). Images were taken via Incucyte S3 (Sartorius ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were then moved to a flat-bottom 96 well plate containing 160 uL of medium supplemented with 500 U/mL IL-2 (Peprotech, Cranbury, NJ) or 10 ng/mL of IL-7 (BioTechne ...